Highly Porous Biphasic Calcium Phosphate (BCP) Ceramics with Large Interconnected Pores by Freezing Vigorously Foamed BCP Suspensions under Reduced Pressure

Nanocomposite structure of the bone can be mimicked by chitosan/hydroxyapatite (CS/HAp) composite scaffold. Biological hydroxyapatite (HAp) contains various ions, which have a crucial role in bone growth. The aim of the present work was to synthesize biomimetic hydroxyapatite and prepare composite scaffolds based on chitosan, where HAp was synthesised from hen eggshells, seashells and cuttlefish bone. The powders were composed of nano-structured calcium deficient HAp and amorphous calcium phosphate (ACP). In the as-prepared powders, Sr2+, Mg2+ and Na+ ions were detected as a result of using biogenic precursor of Ca2+ ions. Highly porous CS/HAp structures have been prepared by freeze-gelation technique. The CS/HAp scaffolds have shown highly porous structure with very well interconnected pores and homogeneously dispersed HAp particles. The MTT assay of CS/HAp scaffolds has shown no toxicity, and the live/dead assay has confirmed good viability and proliferation of seeded cells.

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Fully Interconnected Globular Porous Biphasic Calcium Phosphate Ceramic Scaffold Facilitates Osteogenic Repair

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.119 ◽

2007 ◽

Vol 361-363 ◽

pp. 119-122 ◽

Cited By ~ 4

Author(s):

J.H. Lim ◽

J.H. Park ◽

Eui Kyun Park ◽

Hae Jung Kim ◽

Il Kyu Park ◽

...

Keyword(s):

Tissue Engineering ◽

Calcium Phosphate ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Bone Substitute ◽

Biphasic Calcium Phosphate ◽

Bone Defects ◽

Calcium Phosphate Ceramic ◽

Ceramic Scaffold ◽

Porous Biphasic Calcium Phosphate

An appropriate scaffold, which provides structural support for transplanted cells and acts as a vehicle for the delivery of biologically active molecules, is critical for tissue engineering. We developed a fully interconnected globular porous biphasic calcium phosphate ceramic scaffold by adopting a foaming method, and evaluated its efficiency as a bone substitute and a scaffold for bone tissue engineering by in vitro and in vivo biocompatible analysis and its osteogenic healing capacity in rat tibial bone defects. They have spherical pores averaging 400um in diameter and interconnecting interpores averaging 70um in diameter with average 85% porosity. They elicited no cytotoxicity and noxious effect on cellular proliferation and osteoblastic differentiation during the cell-scaffold construct formation. Also the bone defects grafted with fully interconnected globular porous biphasic calcium phosphate ceramic blocks revealed excellent bone healing within 3 weeks. These findings suggest that the fully interconnected porous biphasic calcium phosphate scaffold formed by the foaming method can be a promising bone substitute and a scaffold for bone tissue engineering.

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Fabrication of Porous Structure of BCP Sintered Bodies Using Microwave Assisted Synthesized HAp Nano Powder

Materials Science Forum ◽

10.4028/www.scientific.net/msf.534-536.49 ◽

2007 ◽

Vol 534-536 ◽

pp. 49-52 ◽

Cited By ~ 13

Author(s):

Min Ho Youn ◽

Rajat Kanti Paul ◽

Ho Yeon Song ◽

Byong Taek Lee

Keyword(s):

Calcium Phosphate ◽

Porous Structure ◽

Relative Density ◽

Polymethyl Methacrylate ◽

Biphasic Calcium Phosphate ◽

Sintering Temperature ◽

Microwave Assisted ◽

Nano Powder ◽

Maximum Value ◽

Porous Biphasic Calcium Phosphate

Using microwave synthesized HAp nano powder and polymethyl methacrylate (PMMA) as a pore-forming agent, the porous biphasic calcium phosphate (BCP) ceramics were fabricated depending on the sintering temperature. The synthesized HAp powders was about 70-90 nm in diameter. In the porous sintered bodies, the pores having 150-180 μm were homogeneously dispersed in the BCP matrix. Some amounts of pores interconnected due the necking of PMMA powders which will increase the osteoconductivity and ingrowth of bone-tissues while using as a bone substrate. As the sintering temperature increased, the relative density increased and showed the maximum value of 79.6%. From the SBF experiment, the maximum resorption of Ca2+ ion was observed in the sample sintered at 1000°C.

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