Reduced invasion to human epithelial cell lines of Salmonella enterica serovar Typhi carrying S. Typhimurium sopD2

bcl-2 gene expression confers a survival advantage by preventing cells from entering apoptosis. In contrast to the previously described cytoplasmic localization of Bcl-2 in epithelial cells in vivo, in this study we have demonstrated, in a series of human epithelial cell lines, that Bcl-2 also localizes to mitotic nuclei. Both immunocytochemical and immunoelectron microscopical examinations localize this protein to nuclei and in particular to chromosomes. Nuclear Bcl-2 expression in these cell lines is correlated with the cell cycle. There is relatively strong expression during mitosis, most intense during prophase and metaphase, declining in telophase and then the protein becomes undetectable soon after separation of the two daughter cells. The expression and distribution of Bcl-2 is influenced by treatment with excessive thymidine. These results indicate that Bcl-2 may protect the cells from apoptosis occurring during mitosis and suggest a possible role for the protein in cell immortalization.

Download Full-text

Interaction of prostate carcinoma-associated fibroblasts with human epithelial cell lines in vivo

Differentiation ◽

10.1016/j.diff.2017.07.002 ◽

2017 ◽

Vol 96 ◽

pp. 40-48 ◽

Cited By ~ 14

Author(s):

Takeshi Sasaki ◽

Omar E. Franco ◽

Simon W. Hayward

Keyword(s):

Epithelial Cell ◽

Cell Lines ◽

Prostate Carcinoma ◽

Human Epithelial Cell ◽

Epithelial Cell Lines ◽

Carcinoma Associated Fibroblasts

Download Full-text

Efficient gene transfer into human epithelial cell lines using glycosylated cationic carriers and neutral glycosylated co-lipids

Blood Cells Molecules and Diseases ◽

10.1016/j.bcmd.2003.11.004 ◽

2004 ◽

Vol 32 (2) ◽

pp. 271-282 ◽

Cited By ~ 15

Author(s):

T Montier

Keyword(s):

Gene Transfer ◽

Epithelial Cell ◽

Cell Lines ◽

Human Epithelial Cell ◽

Efficient Gene ◽

Epithelial Cell Lines

Download Full-text

Genes of non-typeable Haemophilus influenzae expressed during interaction with human epithelial cell lines

Molecular Microbiology ◽

10.1046/j.1365-2958.2002.03025.x ◽

2002 ◽

Vol 45 (2) ◽

pp. 485-500 ◽

Cited By ~ 12

Author(s):

Peter van Ulsen ◽

Muriel van schilfgaarde ◽

Jacob Dankert ◽

Henk Jansen ◽

Loek van Alphen

Keyword(s):

Epithelial Cell ◽

Haemophilus Influenzae ◽

Cell Lines ◽

Human Epithelial Cell ◽

Epithelial Cell Lines

Download Full-text

Cell- and inducer-specific accretion of human isometallothioneins

Biochemical Journal ◽

10.1042/bj2920551 ◽

1993 ◽

Vol 292 (2) ◽

pp. 551-554 ◽

Cited By ~ 16

Author(s):

M Cavigelli ◽

J H R Kägi ◽

P E Hunziker

Keyword(s):

Epithelial Cell ◽

Cell Lines ◽

Dose Response ◽

Zinc Ions ◽

Human Epithelial Cell ◽

Germ Layers ◽

Neutral Ph ◽

Epithelial Cell Lines

The synthesis of metallothionein (MT) was investigated in three different human epithelial cell lines, each derived from one of the embryonic germ layers. The accretion of different isoforms of the protein was monitored using a sensitive neutral-pH h.p.l.c. method. Induction of MT synthesis by zinc ions and dexamethasone revealed differences between the three cell lines, both with respect to the number and the amounts of the different isoMTs formed. Dose-response experiments showed that an increase in dexamethasone concentration enhances MT accretion asymptotically to a limit, whereas within the concentration range explored zinc produces an exponential augmentation.

Download Full-text