The histone acetyl transferase LdHAT1 fromLeishmania donovaniis regulated by S-phase cell cycle kinase

ObjectiveAplasia Ras homolog member I (ARHI) is associated with human ovarian cancer (HOC) growth and proliferation; however, the mechanisms are unclear. The purpose of this study was to investigateARHIeffects in HOC SKOV3 cells.MethodsWe transfected SKOV3 cells with PIRES2-EGFP-ARHI and measured growth inhibition rates, cell cycle distribution, apoptosis rates, and expression of P-STAT3 (phosphorylated signal transduction and activators of transcription 3) and P-ERK (phosphorylated extracellular signal regulated protein kinase).ResultsOur data showed significant inhibition of growth, significantly increased S-phase arrest and apoptosis rates, and reduction of P-STAT3 and P-ERK1/2 expression levels.ConclusionsWe propose the mechanism may involveARHI-induced phosphorylation of ERK1/2 and STAT3 protein kinases, thereby blocking proliferation signaling pathways, to induce HOC SKOV3 apoptosis.

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Cdc5L interacts with ATR and is required for the S‐phase cell‐cycle checkpoint

EMBO Reports ◽

10.1038/embor.2009.122 ◽

2009 ◽

Vol 10 (9) ◽

pp. 1029-1035 ◽

Cited By ~ 51

Author(s):

Nianxiang Zhang ◽

Ramandeep Kaur ◽

Shamima Akhter ◽

Randy J Legerski

Keyword(s):

Cell Cycle ◽

S Phase ◽

Cell Cycle Checkpoint ◽

Phase Cell ◽

Cycle Checkpoint

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Xanthohumol Induces Growth Inhibition and Apoptosis in Ca Ski Human Cervical Cancer Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2015/921306 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 13

Author(s):

Wai Kuan Yong ◽

Sri Nurestri Abd Malek

Keyword(s):

Cell Cycle ◽

Cervical Cancer ◽

Cancer Cells ◽

Cancer Cell ◽

Morphological Changes ◽

S Phase ◽

Phase Cell ◽

Cervical Cancer Cell Line ◽

Dependent Manner ◽

Cervical Cancer Cells

We investigate induction of apoptosis by xanthohumol on Ca Ski cervical cancer cell line. Xanthohumol is a prenylated chalcone naturally found in hop plants, previously reported to be an effective anticancer agent in various cancer cell lines. The present study showed that xanthohumol was effective to inhibit proliferation of Ca Ski cells based on IC50values using sulforhodamine B (SRB) assay. Furthermore, cellular and nuclear morphological changes were observed in the cells using phase contrast microscopy and Hoechst/PI fluorescent staining. In addition, 48-hour long treatment with xanthohumol triggered externalization of phosphatidylserine, changes in mitochondrial membrane potential, and DNA fragmentation in the cells. Additionally, xanthohumol mediated S phase arrest in cell cycle analysis and increased activities of caspase-3, caspase-8, and caspase-9. On the other hand, Western blot analysis showed that the expression levels of cleaved PARP, p53, and AIF increased, while Bcl-2 and XIAP decreased in a dose-dependent manner. Taken together, these findings indicate that xanthohumol-induced cell death might involve intrinsic and extrinsic apoptotic pathways, as well as downregulation of XIAP, upregulation of p53 proteins, and S phase cell cycle arrest in Ca Ski cervical cancer cells. This work suggests that xanthohumol is a potent chemotherapeutic candidate for cervical cancer.

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Cloning of a human S-phase cell cycle gene: use of transient expression for screening

Molecular and Cellular Biology ◽

10.1128/mcb.7.2.775-779.1987 ◽

1987 ◽

Vol 7 (2) ◽

pp. 775-779

Author(s):

A Fainsod ◽

G Diamond ◽

M Marcus ◽

F H Ruddle

Keyword(s):

Cell Cycle ◽

Genomic Library ◽

Chinese Hamster ◽

Chinese Hamster Cell ◽

S Phase ◽

Phase Cell ◽

Cell Cycle Gene ◽

Temperature Sensitive ◽

Specific Cell ◽

Restrictive Temperature

We report here the cloning of a human cell cycle gene capable of complementing a temperature-sensitive (ts) S-phase cell cycle mutation in a Chinese hamster cell line. Cloning was performed as follows. A human genomic library in phage lambda containing 600,000 phages was screened with labeled cDNA synthesized from an mRNA fraction enriched for the specific cell cycle gene message. Plaques containing DNA inserts which hybridized to the cDNA were picked, and their DNAs were assayed for transient complementation in DNA transformation experiments. The transient complementation assay we developed is suitable for most cell cycle genes and indeed for many genes whose products are required for cell proliferation. Of 845 phages screened, 1 contained an insert active in transient complementation of the ts cell cycle mutation. Introduction of this phage into the ts cell cycle mutant also gave rise to stable transformants which grew normally at the restrictive temperature for the ts mutant cells.

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