ORIGINAL ARTICLE: Follicular Fluid Levels of Interleukin-10 and Interferon-gamma do not Predict Outcome of Assisted Reproductive Technologies

2008 ◽  
Vol 59 (2) ◽  
pp. 118-126 ◽  
Author(s):  
Zivile Cerkiene ◽  
Audrone Eidukaite ◽  
Audrone Usoniene
Keyword(s):  
Interferon Gamma ◽  
Follicular Fluid ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Interleukin 10 ◽  
Fluid Levels

176 IN VITRO MATURATION OF DOG OOCYTES IN CANINE FOLLICULAR FLUID

2014 ◽  
Vol 26 (1) ◽  
pp. 202
Author(s):  
K. Reynaud ◽  
S. Canguilhem ◽  
S. Thoumire ◽  
S. Chastant-Maillard
Keyword(s):  
Follicular Fluid ◽  
In Vitro Maturation ◽  
Assisted Reproductive Technologies ◽  
Cumulus Cells ◽  
Reproductive Technologies ◽  
Control Group ◽  
Limiting Factor ◽  
Cytoplasmic Maturation

In the canine species, assisted reproductive technologies, especially in vitro maturation (IVM) and IVF, are still ineffective. The main limiting factor remains the immaturity of the oocytes collected from anestrus ovaries. The ability of an oocyte to reach the MII stage in vitro is linked to the diameter of its follicle and anestrus oocytes, collected from small (<1 mm) follicles, are profoundly immature (De Lesegno et al. 2008). The objective of this study was to improve cytoplasmic quality by mimicking in vivo conditions; that is, to test the effect of pure preovulatory follicular fluid (FF) on survival and IVM rates of anestrus dog oocytes, in order to improve the nuclear and cytoplasmic maturation of these immature oocytes. Follicular fluids samples were collected from 54 Beagle bitches at 2 stages: before the LH peak (n = 23 bitches) and after the LH peak (n = 31 bitches). Only follicular fluid samples from large (>4 mm) follicles were collected and pooled by stage. Control oocytes were matured in 20% FCS/M199 medium. Groups of 5 oocytes were in vitro matured in 30 μL of follicular fluid, in half-area 96-well plates (5% CO2, 38°C). After 72 h of IVM, oocytes were denuded, fixed, and stained for DNA and tubulin before observation by confocal microscopy, and nuclear stages were classified as GV-A to GV-E, MI, and MII (Reynaud et al. 2012). A total of 460 oocytes were collected from 13 anestrus bitches and allocated to either the control medium (n = 155), the Pre-LH FF (n = 145) or the Post-LH FF (n = 160) groups. After 72 h of IVM, the morphology of the cumulus–oocyte complexes (COC) in the post-LH group was different from that of the others: cumulus cells appeared more compact and darker. Analysis of the nuclear stages showed that the degeneration rate was significantly higher (P < 0.05) in the post-LH group (58.7%) than in the pre-LH (40.9%) or in the control group (34.4%). No significant differences (P > 0.05) were observed between the 3 groups in the rate of immature GVA-B oocytes (36.4, 28.5, and 25.3% in the control, Pre-LH, and Post-LH groups, respectively), in the rate of meiotic resumption (GV-C/D/E, MI, MII stages, 44.4, 51.9, and 38.7% in the control, Pre-LH, and Post-LH groups, respectively). Metaphase II rates were not significantly different (12.1, 8.6, and 4.8% in the control, Pre-LH, and Post-LH groups, respectively). In conclusion, canine COC may survive when exposed to IVM in pure follicular fluid, but the degeneration rate was higher in the post-LH group. The presence of follicular fluid did not inhibit meiosis resumption, but did not significantly improve IVM rates. To better mimic in vivo conditions, IVM in a sequence of media, such as IVM in follicular fluid followed by IVM in oviducal fluid remains to be tested.

scholarly journals The level of melatonin in the blood and follicular fluid in women with infertility in the program of assisted reproductive technologies and the effectiveness of its application

2021 ◽  
Vol 25 (2(98)) ◽  
pp. 119-124
Author(s):  
V. Yuzko
Keyword(s):  
Follicular Fluid ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Control Group ◽  
Healthy Children ◽  
Art Programs ◽  
The Third ◽  
Women In Art ◽  
Ovulation Stimulation ◽  
Female Donor

Objective was to investigate the level of melatonin in the blood and follicular fluid in women treated with infertility by ART method and evaluate the effectiveness of melatonin in their preparation for programs.Material and methods. 89 women were examined. The first (control) group included 13 healthy women oocyte donors who gave birth to their own healthy children, the second group - 33 women with infertility, who two weeks before and during ovulation stimulation were taken simultaneously at the same time before bedtime 3 mg of the drug "Vita-melatonin" produced by "Kyiv Vitamin Plant", the third group - 43 women with infertility who did not take the drug melatonin during ovulation stimulation. ELISA (Germany) reagent kits were used to determine melatonin levels. Melatonin levels were determined in blood plasma, and follicular fluid obtained during the puncture at 9:00 am.Results. The level of melatonin in the blood of female donor oocytes was 130.85 ± 16.91 pg/ml. This rate in the blood of women who used the drug melatonin before and during ovulation stimulation was significantly higher than in the blood of women who did not take the drug (respectively, 143.06 ± 14.87 pg/ml and 123.40 ± 12.65 pg / ml, p <0.05), and in the follicular fluid there was an inverse relationship: the level of melatonin in women of the first group was 97.15 ± 8.69 pg / ml, the second group - 39.46 ± 4.52 pg/ml, which is significantly less (p <0.05), the third group - 62.34 ± 3.94 pg / ml, which is almost twice more (p <0.05) compared with women who took melatonin, but less (p <0.05) compared with the first group. The frequency of pregnancy on transfer in patients of the first group was 80.0 ± 11.01%, in women of the second group probably less - 60.6 ± 8.25% (p <0.05), but also probably higher compared to women in the third group - 45.0 ± 7.62% (p <0.05). A similar pattern was observed by us on the onset of pregnancy in general: in the first group - 100.0%, in the second - 68.9 ± 8.05% (p <0.05), in the third - 60.5 ± 7.47% (p> 0.05).Conclusions. The low level of melatonin in the follicular fluid obtained by us in women who underwent ovulation stimulation in ART programs in the treatment of infertility requires further scientific interpretation. At the same time, the best results of pregnancy in women in ART programs who took melatonin in preparation are encouraging.

scholarly journals Predictive value of ovarian hormone-producing function assessment in poor responders undergoing assisted reproductive technologies

2020 ◽  
Vol 68 (6) ◽  
pp. 7-18
Author(s):  
Alexandra I. Merkulova ◽  
Lyailya Kh. Dzhemlikhanova ◽  
Dariko A. Niauri ◽  
Alexander M. Gzgzyan ◽  
Igor Yu. Kogan ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Ovarian Stimulation ◽  
Ovarian Function ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Fertility Treatment ◽  
Total Testosterone ◽  
Poor Responders ◽  
Aromatase Activity ◽  
Serum Estradiol

Hypothesis/aims of study. The management of poor responders to ovarian stimulation for in vitro fertilization (IVF) has always been a challenge. It is difficult for both doctors and patients to make decision to proceed to oocyte donation or abandon fertility treatment. More predictors of successful IVF treatment in poor responders are needed. The aim of this study was to assess hormonal ovarian function in poor responders undergoing ovarian stimulation and to identify predictors of the chance of clinical pregnancy after IVF cycle. Study design, materials and methods. The study included 45 infertile patients undergoing ovarian stimulation with poor ovarian response according to the Bologna criteria. All patients underwent standard IVF or IVF/ICSI protocol using gonadotropin releasing hormone antagonists. Letrozole (5 mg/day) was administered during the first 5 days of stimulation in standard antagonist FSH/hMG protocol to 13 of the patients included in the study, with standard antagonist FSH/hMG protocol being only administered to other 32 patients. Serum and follicular fluid were collected at the time of follicle aspiration, and the concentrations of total testosterone, estradiol and androstenedione were determined. Results. Follicular fluid concentrations of testosterone and androstenedione were higher and serum estradiol level was lower in the letrozole group. The serum concentrations of testosterone and androstenedione were comparable in both groups, while the serum estradiol/testosterone ratio was lower in the letrozole group. The threshold level of estradiol/testosterone ratio 1000 on the day of oocyte retrieval above which pregnancy and implantation rates were increased was 1532.68 (odds ratio 7.0 (95% CI 1.3935.35), р = 0.02). Conclusion. Evaluating of the serum estradiol / testosterone ratio has been shown to determine aromatase activity of ovarian preovulatory follicles and to predict IVF outcome in poor responders undergoing assisted reproductive technology.

scholarly journals Assessment of Oxidative Stress Changes in Serum and Follicular Fluid in Relevance to GnRH rival Protocol in Iraqi Infertile Ladies Undergoing ICSI

2019 ◽  
Vol 1 (5) ◽  
Author(s):  
Fatimah Abdulrazaq Raheem ◽  
Hayder A. L. Mossa ◽  
Wasan A. Abdulhamed ◽  
Liqaa R. Altamimi
Keyword(s):  
Follicular Fluid ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Control Group ◽  
Oxidative Potential ◽  
Significant Difference ◽  
Stress Changes ◽  
Antagonist Group ◽  
Antagonist Protocol

Background: Literary works about the role of rival convention in relationship with oxidative pressure are opposing and there is no reasonable agreement in distributed ripeness articles about the role of opponent reproduction convention with this respect. In reality, a few creators support the presence of expanded oxidative initiated oocyte harm in ladies experiencing ART's; regardless, others have denied such a perception. Hence, the present investigation was arranged so as to think about the conceivable oxidative or hostile to oxidative potential controlled by foe convention. Aim of the study: The aim of the current study was to evaluate the serum and follicular ROS concentration in women undergoing ICSI contrasted between two groups, one receiving conventional antagonist protocol and the other receiving conventional agonist protocol. Patients and Methods: The current case control study included two groups of subfertile women. The study group included 57 women receiving antagonist protocol, whereas, the control group included 10 subfertile women receiving agonist protocol. The study was carried out at High Institute of Infertility Diagnosis and Assisted Reproductive Technologies at Al- Nahrain University. The study started on April 2018 and extended to March 2019. Women in both groups were subjected to ICSI procedure according to procedures and techniques adopted in High Institute of Infertility Diagnosis and Assisted Reproductive Technologies at Al- Nahrain University with a single basic difference concerning the ovarian stimulation protocol. Results: Overall clinical pregnancy rate was 44.7 % being significantly higher in antagonist group in comparison with agonist group 50.9 % versus 10.01 %, respectively (P = 0.019). There was no significant difference in the concentration of ROS at cycle day 2 between antagonist and agonistgroups (P = 0.561). There was highly significant difference in the serum concentration of ROS at day of ova pickup between antagonist and agonist groups (P = 0.009); being less in antagonistgroup and there was highly significant difference in the follicular fluid concentration of ROS between antagonist and agonist groups (P = 0.003); being less in antagonist group. Conclusions: Antagonist protocol is associated with significantly higher positive pregnancy outcome in comparison with agonist protocol because of the anti-oxidant effect attributed to antagonist use.

scholarly journals The problem of "thin" endometrium in reproductive medicine: experience of application of recombinant Interleukin-2 to prepare patients to assisted reproductive technologies programs

2017 ◽  
Vol 66 (4) ◽  
pp. 46-50 ◽  
Author(s):  
Olga V. Lysenko ◽  
Tatyana A. Rozhdzestvenskaya
Keyword(s):  
Assisted Reproductive Technologies ◽  
Interleukin 2 ◽  
Uterine Cavity ◽  
Reproductive Technologies ◽  
Interleukin 10 ◽  
Reproductive Age ◽  
Necrosis Factor Alpha ◽  
Thin Endometrium ◽  
Chronic Endometritis ◽  
Recombinant Interleukin 2

Introduction. “Thin” endometrium is known affect negatively to reproductive function. Treatment of “thin” endometrium remains a problem and subject of future major studies. Aim. To justify the possibility of applying of recombinant interleikina-2 person late reproductive age patients with “thin” endometrium before using assisted reproductive technologies. Matherials and Methods. We examined a total of 64 late reproductive age women. Three groups of patients were analyzed. The first investigated group included 25 women with histologically proven chronic endometritis; the comparison group included 20 patients with endometrium of the early proliferative phase; the second investigated group included 19 patients with “thin” endometrium, with indications for the conduct of in vitro fertilization. Interleukin 2, interleukin-4, tumor necrosis factor alpha, interleukin-1β, Interleukin-6, interleukin-8, interleukin-10, IFN-γ concentrations were determined by enzyme linked immunosorbent assay in serum and aspirates from the uterine cavity in first investigated group and comparison group. Vaginal ultrasonic examination was made for women of second investigated group in first and second phase of menstrual cycle before and after recombinant human interlejkinom-2 treatment. Statistical data processing was performed using application software package Statistica 6.0 (StatSoft, Ink. 1994–2001), adapted for biomedical research. P < 0.05 was considered significant. Results. Were found that concentrations of interleukin 2, interleukin-10 significantly reduced and concentrations of tumor necrosis factor alpha, interleukin-1β, Interleukin-6 and interleukin-8 significantly increased in aspirates from the uterine cavity in women of late reproductive age with chronic endometritis. All changes occur at the local level in chronic endometritis. Endometrial thickness a statistically significant increase after use of recombinant human interlejkinom-2 in patients with infertility and chronic endometritis. Conclusions. Application of recombinant interleukin-2 in patients with “thin” endometrium can become optimal schema therapy, which requires further study.

scholarly journals Serum and follicular fluid leptin levels are correlated with human embryo quality

Reproduction ◽  
2005 ◽  
Vol 130 (6) ◽  
pp. 917-921 ◽  
Author(s):  
Georgios Anifandis ◽  
Eleni Koutselini ◽  
Ioannis Stefanidis ◽  
Vassilios Liakopoulos ◽  
Constantinos Leivaditis ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Embryo Quality ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Serum Samples ◽  
Serum Leptin ◽  
Follicle Maturation ◽  
Sensitive Marker ◽  
Ivf Et

This prospective study was undertaken to reassess the prognostic value of leptin during critical stages of in vitro fertilization-embryo transfer (IVF-ET) and address its role in the functional staging of assisted reproductive technologies at the level of embryo quality. Serum and follicular fluid samples of 100 selected women undergoing the long IVF-ET protocol were collected for leptin and embryo quality determination. The highest serum leptin concentration (52.11 ± 4.27 ng/ml) was observed on ovum pick up day, while follicular fluid leptin was higher than all serum samples examined (62.59 ± 5.73 ng/ml). Serum leptin above 59.48 ± 7.6 ng/ml was associated with ‘poor’ embryo quality and above 56.87 ± 5.52 ng/ml with pregnancy failure. Elevated leptin concentrations were associated with reduced ovarian stimulation and response, follicle maturation, embryo quality and pregnancy success. Our findings suggest that leptin modulates embryo quality and may serve as a sensitive marker of IVF outcome.

Re-imagining Reproduction: Unsettling Metaphors in the History of Imperial Science and Commercial Surrogacy in India

Somatechnics ◽  
2015 ◽  
Vol 5 (1) ◽  
pp. 88-103 ◽  
Author(s):  
Kalindi Vora
Keyword(s):  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
The Body ◽  
Body Parts ◽  
Colonial History ◽  
Women's Bodies ◽  
Commercial Surrogacy ◽  
Art Practices ◽  
History Of ◽  
Travel Industry

This paper provides an analysis of how cultural notions of the body and kinship conveyed through Western medical technologies and practices in Assisted Reproductive Technologies (ART) bring together India's colonial history and its economic development through outsourcing, globalisation and instrumentalised notions of the reproductive body in transnational commercial surrogacy. Essential to this industry is the concept of the disembodied uterus that has arisen in scientific and medical practice, which allows for the logic of the ‘gestational carrier’ as a functional role in ART practices, and therefore in transnational medical fertility travel to India. Highlighting the instrumentalisation of the uterus as an alienable component of a body and subject – and therefore of women's bodies in surrogacy – helps elucidate some of the material and political stakes that accompany the growth of the fertility travel industry in India, where histories of privilege and difference converge. I conclude that the metaphors we use to structure our understanding of bodies and body parts impact how we imagine appropriate roles for people and their bodies in ways that are still deeply entangled with imperial histories of science, and these histories shape the contemporary disparities found in access to medical and legal protections among participants in transnational surrogacy arrangements.

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