Decreased insulin-like growth factor binding protein-4 (IGFBP-4) mRNA levels in the liver after hypophysectomy

Purpose. To assess the expression of insulin-like growth factor binding protein (IGFBP) family and its prognostic impact in ovarian cancer (OC) patients. Materials and Methods. The mRNA expression and protein expression of individual IGFBPs in healthy ovarian samples and OC tissues were explored through Oncomine, Gene Expression Profiling Interactive Analysis, and Human Protein Atlas database. Additionally, the prognostic values of the six IGFBP members in patients with OC were evaluated by Kaplan-Meier plotter. Results. IGFBP2 and IGFBP4 mRNA expression were remarkably upregulated in patients with OC. To be specific, the mRNA expression of IGFBP2 was upregulated in patients with serous ovarian cancer (SOC), while IGFBP1/3/4/5/6 mRNA levels were downregulated. In addition, the IGFBP4 protein expression was upregulated in SOC, and the IGFBP6 protein expression was upregulated in both of SOC and endometrioid ovarian cancer (EOC) tissues. High IGFBP1 mRNA levels showed favorable overall survival (OS) and progression-free survival (PFS) in all OC. Meanwhile, increased IGFBP5/6 mRNA levels revealed worsen OS and PFS in all OC patients. IGFBP4/6 mRNA levels predicted unfavorable OS and PFS only in SOC patients. Moreover, the aberrant mRNA expression of IGFBP1/2/4/5/6 was correlated with significantly prognosis in patients receiving different chemotherapeutic regimens. Conclusion. This study indicates that the IGFBP family reveals distinct prognosis in patients with OC. IGFBP1/2/4/5/6 are useful prognostic predictors for chemotherapeutic effect in OC patients, and IGFBP2/4 are potential tumor markers for the diagnosis of OC.

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Messenger RNA levels of growth factors, ligands, receptors, and proteins affecting lipid metabolism in pigs

Canadian Journal of Animal Science ◽

10.4141/a00-029 ◽

2000 ◽

Vol 80 (4) ◽

pp. 559-567 ◽

Cited By ~ 1

Author(s):

Claude Robert ◽

Marie-France Palin ◽

Frederick G. Silversides ◽

Robert M. Mckay ◽

Ghislain Pelletier

Keyword(s):

Growth Factor ◽

Binding Protein ◽

Low Density Lipoprotein ◽

Growth Factor Receptor ◽

Density Lipoprotein ◽

Backfat Thickness ◽

Mrna Levels ◽

Insulin Like Growth Factor ◽

Factor Binding ◽

Density Lipoprotein Receptor

The Northern blot technique was used for mRNA phenotyping of 19 growth factors, ligands, receptors, and proteins involved in lipid metabolism in two populations of pigs with different fat deposition capabilities. The mRNA levels were measured in backfat, liver, and muscle tissue at different slaughter weights, taking backfat thickness, gender and breed of the animals into consideration. Of all the RNA patterns measured in the Landrace population, only the mRNA transcript level of low density lipoprotein receptor-related protein (also called alpha 2-macroglobulin receptor) was associated with the pig's backfat thickness phenotype in muscle and backfat tissues. In the population composed of purebred Yorkshire and Hampshire, epidermal growth factor receptor, malic enzyme, platelet derived growth factor β and insulin-like growth factor binding protein 3 show different mRNA patterns associated with backfat thickness phenotypes. When analyzing the data using the gender or the breed as the main effect, the insulin receptor and insulin-like growth factor binding protein 1 were different between genders whereas insulin-like growth factor binding protein 3, malic enzyme, epidermal growth factor receptor and low density lipoprotein receptor-related protein were different between breeds. Analysis of this type should be helpful in understanding the regulation of fat deposition. Key words: mRNA levels, marker genes, backfat, pig

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Hypertension increases insulin-like growth factor binding protein-4 mRNA levels in rat aorta.

Hypertension ◽

10.1161/01.hyp.24.6.679 ◽

1994 ◽

Vol 24 (6) ◽

pp. 679-685 ◽

Cited By ~ 12

Author(s):

A Anwar ◽

P Delafontaine

Keyword(s):

Growth Factor ◽

Binding Protein ◽

Rat Aorta ◽

Mrna Levels ◽

Insulin Like Growth Factor ◽

Factor Binding

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Tri-iodothyronine increases insulin-like growth factor binding protein-2 expression in cultured hepatocytes from hypothyroid rats

Journal of Endocrinology ◽

10.1677/joe.0.1610465 ◽

1999 ◽

Vol 161 (3) ◽

pp. 465-474 ◽

Cited By ~ 2

Author(s):

I Demori ◽

C Bottazzi ◽

E Fugassa

Keyword(s):

Growth Factor ◽

Thyroid Hormone ◽

Binding Protein ◽

Mrna Levels ◽

Dependent Manner ◽

Insulin Like Growth Factor ◽

Cultured Hepatocytes ◽

Factor Binding

Previous evidence suggests the existence of a thyroid hormone-IGF axis in the liver and changes in hepatic insulin-like growth factor binding protein (IGFBP) expression in rats with altered thyroid status have been previously reported. The aim of this study was to check if the higher IGFBP-2 mRNA levels observed in liver of hypothyroid rats could be due to a direct effect of thyroid hormone on the IGFBP-2 gene. In our experiments, cultured hepatocytes isolated from normal and hypothyroid adult rats were used. Northern blot analysis revealed barely detectable IGFBP-2 mRNA in normal rat hepatocytes, but easily detectable signal in hypothyroid rat cells. Therefore, the effect of tri-iodothyronine (T3) was investigated using cultured hepatocytes from hypothyroid rats as an in vitro model. The IGFBP-2 message was increased in a dose-dependent manner in hepatocytes cultured for 12-24 h in the presence of T3. A similar increase occurred in accumulation of IGFBP-2 in the culture medium, as measured by RIA. The effect of T3 on IGFBP-2 transcript levels appeared to consist of enhanced gene transcription and was independent of ongoing protein synthesis, but it was completely abolished by the incubation of hepatocytes with insulin. The latter result confirmed the dominant role of insulin in regulating IGFBP-2 expression by cultured hepatocytes. In vivo experiments confirmed an increase in hepatic IGFBP-2 mRNA and serum IGFBP-2 levels in hypothyroid rats and demonstrated, in addition, a significant increase in these measures in T3-treated rats. Taken together, our in vitro and in vivo results support a role for a thyroid hormone-IGF axis in the liver and suggest that other factors, such as insulin, interact in vivo with thryoid hormone in regulating hepatic IGFBP-2 expression.

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Cadmium Cation Increases the Production and mRNA Levels of Insulin-Like Growth Factor-Binding Protein-1 in HepG2

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.60679 ◽

2007 ◽

Vol 71 (5) ◽

pp. 1334-1337 ◽

Cited By ~ 1

Author(s):

Yuichi OISHI ◽

Motoko OHNISHI ◽

Kazuo KOBAYASHI-HATTORI ◽

Toshichika TAKITA ◽

Tadashi NOGUCHI

Keyword(s):

Growth Factor ◽

Binding Protein ◽

Mrna Levels ◽

Insulin Like Growth Factor ◽

Factor Binding

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Differential regulation by TGF-beta 1 and insulin of insulin-like growth factor binding protein-2 in IEC-6 cells

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1995.268.6.e1199 ◽

1995 ◽

Vol 268 (6) ◽

pp. E1199-E1204

Author(s):

Y. S. Guo ◽

C. M. Townsend ◽

G. F. Jin ◽

R. D. Beauchamp ◽

J. C. Thompson

Keyword(s):

Growth Factor ◽

Inhibitory Action ◽

Binding Protein ◽

High Dose ◽

Mrna Levels ◽

Insulin Like Growth Factor ◽

Tgf Beta ◽

Factor Binding ◽

Growth Inhibitory ◽

Igf I

The purposes of this study were to determine the regulation of insulin-like growth factor binding protein-2 (IGFBP-2) in IEC-6 cells by transforming growth factor-beta 1 (TGF-beta 1) and insulin and to determine whether IGFBP-2 mediated the growth-inhibitory action on the cells. Utilizing Western ligand blot analysis, we found that TGF-beta 1 at concentrations of 0.5, 1.0, and 2 ng/ml significantly increased levels of 32-kDa IGFBP in the conditioned medium (CM) of IEC-6 cells in a dose-dependent fashion and that low doses of insulin (1.0 and 5.0 microgram/ml) also increased IGFBP levels in the CM of IEC-6 cells, but a high dose of insulin (10 micrograms/ml) depressed IGFBP release in the CM. Immunoblotting has shown that the IGFBP of 32 kDa was IGFBP-2 and further confirmed the above results. IGFBP-2 mRNA levels were stimulated by TGF-beta 1 (2.0 ng/ml) and suppressed by insulin (5.0 micrograms/ml). In addition, des (1–3) IGF-I (50 ng/ml) and insulin stimulated the proliferation of IEC-6 cells. Anti-IGFBP-2 antibodies partially blocked the inhibitory role in IEC-6 cell growth evoked by des (1–3) IGF-I. These findings suggest that the upregulation of IGFBP-2 by TGF-beta 1 occurs, at least in part, at the level of mRNA, whereas the regulation by insulin appears to be at a posttranslational level, and that the TGF-beta 1-stimulated production of IGFBP may contribute to the growth-inhibitory action in intestinal epithelial cells.x

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The Regulatory Role of Apelin on the Appetite and Growth of Common Carp (Cyprinus Carpio L.)

Animals ◽

10.3390/ani10112163 ◽

2020 ◽

Vol 10 (11) ◽

pp. 2163

Author(s):

Xiao Yan ◽

Chaobin Qin ◽

Guokun Yang ◽

Dapeng Deng ◽

Liping Yang ◽

...

Keyword(s):

Growth Factor ◽

Food Intake ◽

Common Carp ◽

Binding Protein ◽

Mrna Levels ◽

Insulin Like Growth Factor ◽

Factor Binding ◽

Common Carp Cyprinus Carpio ◽

Carp Cyprinus Carpio

Apelin, a kind of active polypeptide, has many biological functions, such as promoting food intake, enhancing immunity, and regulating energy balance. In mammals, studies have indicated that apelin is involved in regulating food intake. However, there are relatively few studies about the regulatory effect of apelin on fish feeding, and the specific mechanism is not clear. Therefore, the purpose of this study was to preliminarily investigate the regulatory effects of apelin on key genes of feeding and growth in common carp (Cyprinus Carpio L.) through in vitro and in vivo experiments. In the present study, after incubation with different concentrations of Pyr-apelin-13 (0, 10, 100, and 1000 nM) in hypothalamic fragments, the expressions of Neuropeptide Y (NPY) and Agouti related peptide (AgRP) mRNA were significantly up-regulated at 12 and 3 h, respectively, and the significant down-regulation of Cocaine and amphetamine-related transcript (CART) mRNA expression was observed at 1 and 3 h. In vivo, after Pyr-apelin-13 oral administration (0, 1, 10, and 100 pmol/g), the orexin mRNA level in the hypothalamus of common carp was significantly increased at 1, 6, and 12 h, while CART/(Proopiomelanocortin) POMC mRNA levels in the hypothalamus of common carp were significantly down-regulated. Following incubation with different concentrations of Pyr-apelin-13 (0, 10, 100, and 1000 nM) in primary hepatocytes, GHR (Growth hormone receptor), IGF2 (Insulin-like growth factor 2), IGFBP2 (Insulin like growth factor binding protein 2), and IGFBP3 (Insulin like growth factor binding protein 3) mRNA levels were significantly increased at 3 h. In vivo, the levels of IGF1 (Insulin-like growth factor 1), IGF2, IGFBP2 (Insulin like growth factor binding protein 2), and IGFBP3 mRNA were significantly increased after the oral administration of Pyr-apelin-13 in the hepatopancreas, in a time and dose-dependent manner. These results support the hypothesis that Pyr-apelin-13 might regulate the feeding and growth of common carp through mediating the expressions of appetite- and growth-related genes. Overall, apelin, which is an orexigenic peptide, improves food intake and is involved in the growth of common carp.

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