Characterization of the Photosynthetic Activity of Platinized Chloroplasts: A Study Using Fluorescence and Photoacoustic Techniques

There are few studies in the literature that have aimed at characterizing the physical, chemical, and microbial aspects of scum produced in UASB reactors. In addition, there is little information on the influence of operational conditions of UASB reactors on scum formation, and the present work addresses these issues. Three demo-scale UASB reactors, fed on domestic wastewater, were employed to monitor the formation and its characteristics. Scum production was periodically assessed during different operational phases, and its characterization involved analyses of BOD, COD, solids, sulfide, sulfate, microscopic observations, as well as biodegradability tests. The results show that the scum formed was physically, chemically, and microscopically similar in both geminated reactors, being comprised mainly of organic material of low biodegradability. Several bacterial morphotypes, mainly filaments and rods, with internal sulfur granules, were observed, and the aerobic microorganisms that developed at the scum layer as a result of photosynthetic activity of cyanobacteria, seemed to play an important role in sulfide removal and odour control. Scum production rates were similar in both reactors, but the imposed higher upflow velocities resulted in a higher production rate and in a reduced biodegradability of the scum.

Download Full-text

Noninvasive characterization of glucose aqueous solutions based on continuous-wave photoacoustic techniques: A phantom-based approach

2013 IEEE SENSORS ◽

10.1109/icsens.2013.6688257 ◽

2013 ◽

Author(s):

S. Camou ◽

D. Stevens ◽

Y. Higuchi ◽

H. Koizumi

Keyword(s):

Aqueous Solutions ◽

Continuous Wave ◽

Noninvasive Characterization ◽

Photoacoustic Techniques

Download Full-text

Characterization of light-absorbing aerosols from a laboratory combustion source with two different photoacoustic techniques

Aerosol Science and Technology ◽

10.1080/02786826.2020.1849537 ◽

2020 ◽

pp. 1-14

Author(s):

Zhenhong Yu ◽

Zezhen Cheng ◽

Gregory R. Magoon ◽

Omar El Hajj ◽

Rawad Saleh

Keyword(s):

Combustion Source ◽

Absorbing Aerosols ◽

Photoacoustic Techniques

Download Full-text

An isoform of the plastid RNA polymerase-associated protein FSD3 negatively regulates chloroplast development

BMC Plant Biology ◽

10.1186/s12870-019-2128-9 ◽

2019 ◽

Vol 19 (1) ◽

Author(s):

Sangyool Lee ◽

Young Hee Joung ◽

Ju-Kon Kim ◽

Yang Do Choi ◽

Geupil Jang

Keyword(s):

Rna Polymerase ◽

Photosynthetic Activity ◽

Chloroplast Development ◽

Transmembrane Domain ◽

Developmental Defects ◽

Expression Of Genes ◽

Associated Proteins ◽

Plastid Rna Polymerase ◽

Chloroplast Nucleoid

Abstract Background Plastid-encoded RNA polymerase (PEP) plays an essential role in chloroplast development by governing the expression of genes involved in photosynthesis. At least 12 PEP-associated proteins (PAPs), including FSD3/PAP4, regulate PEP activity and chloroplast development by modulating formation of the PEP complex. Results In this study, we identified FSD3S, a splicing variant of FSD3; the FSD3 and FSD3S transcripts encode proteins with identical N-termini, but different C-termini. Characterization of FSD3 and FSD3S proteins showed that the C-terminal region of FSD3S contains a transmembrane domain, which promotes FSD3S localization to the chloroplast membrane but not to nucleoids, in contrast to FSD3, which localizes to the chloroplast nucleoid. We also found that overexpression of FSD3S negatively affects photosynthetic activity and chloroplast development by reducing expression of genes involved in photosynthesis. In addition, FSD3S failed to complement the chloroplast developmental defects in the fsd3 mutant. Conclusion These results suggest FSD3 and FSD3S, with their distinct localization patterns, have different functions in chloroplast development, and FSD3S negatively regulates expression of PEP-dependent chloroplast genes, and development of chloroplasts.

Download Full-text

Characterization of Co-Cultivation of Cyanobacteria on Growth, Productions of Polysaccharides and Extracellular Proteins, Nitrogenase Activity, and Photosynthetic Activity

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-016-2215-4 ◽

2016 ◽

Vol 181 (1) ◽

pp. 340-349 ◽

Cited By ~ 3

Author(s):

Chuizhao Xue ◽

Libo Wang ◽

Tong Wu ◽

Shiping Zhang ◽

Tao Tang ◽

...

Keyword(s):

Photosynthetic Activity ◽

Nitrogenase Activity ◽

Extracellular Proteins

Download Full-text

Morphological Characterization of Mycobacteriophage R1

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051281 ◽

1974 ◽

Vol 32 ◽

pp. 254-255

Author(s):

B. L. Soloff ◽

T. A. Rado

Keyword(s):

Carbon Source ◽

Stationary Phase ◽

Growth Phase ◽

Minimal Medium ◽

Morphological Characterization ◽

Logarithmic Growth Phase ◽

Complete Lysis ◽

Lysogenic Culture ◽

Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

Download Full-text

AEM analysis of crystallization in Ti-based amorphous alloys

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100075142 ◽

1983 ◽

Vol 41 ◽

pp. 270-271

Author(s):

A.R. Pelton ◽

A.F. Marshall ◽

Y.S. Lee

Keyword(s):

Magnetic Properties ◽

Amorphous Alloys ◽

Amorphous Materials ◽

Isothermal Annealing ◽

Amorphous Matrix ◽

Nucleation And Growth ◽

Current Interest ◽

Amorphous Films ◽

Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Download Full-text