Cytokine profile in tear and aqueous humor of primary open‐angle glaucoma patients as a prognostic factor for trabeculectomy outcome

Objective To evaluate the cytokine profile in tear and aqueous humor in primary open-angle glaucoma before trabeculectomy and correlate preoperative cytokine levels with the surgical outcome. Methods Prospective study. Twenty-nine patients with primary open-angle glaucoma undergoing primary trabeculectomy were included. Levels of 27 cytokines were measured in tear an aqueous humor using the Bio-Plex Pro Human Cytokine 27-Plex Immunoassay kit (Bio-Rad Laboratories, Hercules, CA, USA). Results 29 patients who underwent trabeculectomy were included and their first-year follow-up visits were recorded. Mean age was 76.0 ± 7.0 years (range 56–84), mean intraocular pressure was 18.2 ± 3.6 mmHg and mean number of topical medications was 2.3 ± 0.9. At the one-year visit, 5 patients were classified as surgical failure. In aqueous humor, preoperative cytokine levels of regulated on activation normal T cell expressed and secreted (RANTES) were significantly higher in those patients with surgical failure at one year. IL-8 in tear and interferon gamma-induced protein (IP-10) in aqueous humor correlated positively with one-year IOP reduction. No statistically significant correlations were found with changes in visual field mean defect or global peripapillary retinal nerve fiber layer thickness (all, p >0.05). Conclusions Preoperative RANTES levels in aqueous humor as well as other cytokines could serve as useful biomarkers for trabeculectomy outcome.

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Detection of specific proteins in the aqueous humor in primary open-angle glaucoma

Korean Journal of Ophthalmology ◽

10.3341/kjo.1990.4.1.1 ◽

1990 ◽

Vol 4 (1) ◽

pp. 1 ◽

Cited By ~ 5

Author(s):

In Seop Lee ◽

Young Suk Yu ◽

Dong Myung Kim ◽

Dong Ho Youn ◽

Jin Q Kim

Keyword(s):

Aqueous Humor ◽

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Open Angle ◽

Specific Proteins

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WITHDRAWN: Transforming growth factor-β2 levels in the aqueous humor of patients with primary open angle glaucoma and primary angle closure glaucoma

Clinica Chimica Acta ◽

10.1016/j.cca.2010.11.018 ◽

2010 ◽

Author(s):

Jasbir Kaur ◽

Parul Saxena ◽

Arpna Srivastava ◽

Ramanjit Sihota ◽

Reena Sharma

Keyword(s):

Growth Factor ◽

Aqueous Humor ◽

Transforming Growth Factor ◽

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Angle Closure ◽

Open Angle ◽

Primary Angle Closure ◽

Primary Angle Closure Glaucoma ◽

Transforming Growth Factor Β2

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Multiplex Cytokine Analysis of Aqueous Humor in Eyes with Primary Open-Angle Glaucoma, Exfoliation Glaucoma, and Cataract

Investigative Opthalmology & Visual Science ◽

10.1167/iovs.11-8434 ◽

2012 ◽

Vol 53 (1) ◽

pp. 241 ◽

Cited By ~ 93

Author(s):

Yasuyuki Takai ◽

Masaki Tanito ◽

Akihiro Ohira

Keyword(s):

Aqueous Humor ◽

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Open Angle ◽

Exfoliation Glaucoma ◽

Cytokine Analysis ◽

Multiplex Cytokine

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Profile of vascular and inflammatory aqueous humor cytokines in primary open angle glaucoma patients

10.21203/rs.2.164/v1 ◽

2019 ◽

Author(s):

Ruyi Zhai ◽

Huan Xu ◽

Xinghuai Sun ◽

Xiangmei Kong

Keyword(s):

Aqueous Humor ◽

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Antibody Array ◽

Open Angle ◽

Aqueous Samples ◽

Age Related ◽

Tnf Α ◽

Cytokine Antibody Array ◽

Angiopoietin 2

Abstract Purpose: This study was aimed to analyze the profile of vascular and inflammatory cytokines in aqueous humor of primary open angle glaucoma (POAG) patients. Methods: Aqueous humor samples were acquired from 6 POAG patients and 7 age-related cataract patients that constituted the control group. A human cytokine antibody array was used to detect 37 proteins related to inflammation and vascular regulation. Characteristics including age, gender, and intraocular pressure (IOP) of POAG and age-related cataract patients were compared. Correlation analyses between aqueous humor cytokines and characteristics were performed. Results: The cytokine antibody array results showed that the signal intensities of soluble vascular endothelial growth factor receptor 1 (sVEGFR-1), von Willebrand factor (vWF), tumor necrosis factor (TNF)-α, and angiopoietin-2 in POAG patients were significantly higher in aqueous samples compared to controls (p < 0.05, Student’s test or Mann-Whitney U-test). Among cytokines of all aqueous samples, preoperative IOP was positively associated with expression quantity of sVEGFR-1 (r = 0.647, p = 0.02) and VEGF-A (r = 0.602, p = 0.04) and negatively associated with plasminogen activator inhibitor 1 expression (r = -0.593, p = 0.04). Values for sVEGFR-1 were positively correlated with vWF (r = 0.646, p = 0.02) and TNF-α (r = 0.824, p < 0.001) with statistical significance. Conclusion: Pathogenesis of primary open angle glaucoma may be associated with both vascular dysfunction and inflammatory responses. The vascular factors may include endothelial dysfunction and damaged vascular permeability as indicated by abnormal expression of vWF, sVEGFR-1 and angiopoietin-2. Keywords: POAG, aqueous humor, cytokines, sVEGFR-1, vWF, angiopoietin-2

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Comparison of cytokine/chemokine levels in aqueous humor of primary open-angle glaucoma patients with positive or negative outcome following trabeculectomy

Bioscience Reports ◽

10.1042/bsr20181894 ◽

2019 ◽

Vol 39 (5) ◽

Cited By ~ 4

Author(s):

Beata Gajda-Deryło ◽

Thomas Stahnke ◽

Stephan Struckmann ◽

Gregor Warsow ◽

Kerstin Birke ◽

...

Keyword(s):

Growth Factor ◽

Aqueous Humor ◽

Transforming Growth Factor ◽

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Transforming Growth Factor Β ◽

Positive Outcome ◽

Open Angle ◽

Antifibrotic Therapy ◽

Cytokines And Chemokines

Abstract We aimed to identify differences in cytokine/chemokine levels in the aqueous humor (AH) of primary open-angle glaucoma (POAG) patients who suffered from scarring, compared with POAG patients with no scarring after trabeculectomy surgery. Identification of differently expressed cytokines and chemokines may help to understand scarring and fibrotic processes following trabeculectomy, and to make predictions for the outcome of fistulating surgery in the future. Furthermore, the identification of cell signaling pathways involved in fibrosis offers the opportunity for a more specific antifibrotic therapy with reduced side effects, and an improvement in long-term surgical outcome. Eight samples of AH were collected during trabeculectomy surgery and commercially available cytokine/chemokine arrays were used. Specific, differently expressed proteins (cytokines/chemokines) in AH samples from patients with positive and negative surgery outcomes were detected. These proteins were classified based on their known profibrotic, inflammatory, adhesive, and apoptotic properties. Transforming growth factor β (TGF-β) and vascular endothelial growth factor (VEGF) were among the most important profibrotic cytokines that we detected. Differences in the fold change of protein expression were highly significant between patients after successful and failed trabeculectomy surgery, and these were processed and visualized using ExprEssence software. This pilot study revealed differences in concentrations of cytokines/chemokines in AH between the two examined groups of patients. Our findings suggest that a positive outcome from trabeculectomy is strongly related to an inhibition of the fibrosis process.

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Aqueous humor metabolite profile of pseudoexfoliation glaucoma is distinctive

Molecular Omics ◽

10.1039/c9mo00192a ◽

2020 ◽

Vol 16 (5) ◽

pp. 425-435 ◽

Cited By ~ 1

Author(s):

Ciara Myer ◽

Leila Abdelrahman ◽

Santanu Banerjee ◽

Ram B. Khattri ◽

Matthew E. Merritt ◽

...

Keyword(s):

Machine Learning ◽

Aqueous Humor ◽

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Metabolite Profile ◽

Open Angle ◽

Pseudoexfoliation Glaucoma

We identified 298 metabolites in pseudoexfoliation (PEX) glaucoma, primary open angle glaucoma (POAG) and non-glaucomatous controls. Machine learning can classify aqueous humor into the three distinct categories and presents the opportunity for future predictions.

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