Effect of Polysorbate (Tween) 80 on the resistance of Pseudomonas aeruginosa to chemical inactivation

1964 ◽  
Vol 16 (S1) ◽  
pp. 51T-55T ◽  
Author(s):  
M. R. W. BROWN ◽  
R. M. E. RICHARDS
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tween 80 ◽  
Chemical Inactivation

scholarly journals STUDI PERBANDINGAN BIOSURFAKTAN Pseudomonas aeruginosa IA7d DAN SURFAKTAN SINTETIK Tween-80 DALAM BIODEGRADASI SOLAR OLEH MIKROBA PERAIRAN PELABUHAN TANJUNG PERAK SURABAYA

2006 ◽  
Vol 12 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Ni’matuzahroh Ni’matuzahroh ◽  
Arif Yachya ◽  
Mulyadi Tanjung
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diesel Fuel ◽  
Room Temperature ◽  
Surfactant Solution ◽  
Tween 80 ◽  
Synthetic Surfactant ◽  
Microbial Count ◽  
Crude Product ◽  
And Control ◽  
Biodegradation Test

This research aimed to know the influence of addition crude product Pseudomonas aeruginosa IA7d biosurfactant from substrate hexadecane and diesel fuel in supporting activity of diesel fuel biodegradation and both potention to replace synthetic surfactant for hydrocarbon remediation. Biodegradation test was done by experimental in laboratory scale by Tween-80 as synthetic surfactant comparator. Growth culture was from seawater of Tanjung Perak Harbor Surabaya include various microbe types in its. 2 percent (w/v) of diesel fuel and three surfactants test (0.70 percent w/v crude product P. aeruginosa IA7d biosurfactant from hexadecane substrate; 60,69 percent w/v crude product P. aeruginosa IA7d biosurfactant from diesel fuel substrate; and 0.10 percent w/v synthetic surfactant Tween 80) at uniform surface tension of surfactant solution (54.03 mN/m) were added into 30 ml seawater sample. Cultures were incubated at room temperature (30 °C) during 14 days with shaking (90 rpm). Result of biodegradation test showed the third addition of surfactants test had an effect on acceleration of diesel fuel biodegradation activity that proved at the increased of microbial count (bacterium, yeast, and mould) and decreased of diesel fuel concentration was shown by chromatograms area of diesel fuel. Highest degradation was successively reached by culture got crude product P. aeruginosa IA7d biosurfactant from diesel fuel substrate (88.034 percent), crude product P. aeruginosa IA7d biosurfactant from hexadecane substrate (80.908 percent), synthetic surfactant Tween-80 (54.732 percent), and control that was without addition surfactant test (0.132 percent). Base on number of microbial count, percentage of decreasing chromatogram peaks of diesel fuel from each surfactant test, and price substrate produce (diesel fuel and hexadecane) among both crude product P. aeruginosa IA7d biosurfactant, known that the crude product P. aeruginosa IA7d biosurfactant from diesel fuel substrate had highest ability acceleration to biodegradation activity on diesel fuel and had a potency to replace synthetic surfactant for remediation hydrocarbon application in environment.

scholarly journals Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: the process comparison analysis by statistical approach

2013 ◽  
Vol 67 (4) ◽  
pp. 677-685 ◽  
Author(s):  
Sonja Jakovetic ◽  
Zorica Knezevic-Jugovic ◽  
Sanja Grbavcic ◽  
Dejan Bezbradica ◽  
Natasa Avramovic ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Lipase Activity ◽  
Sunflower Oil ◽  
Hydrolytic Enzymes ◽  
Tween 80 ◽  
Lipase Production ◽  
Fermentation Time ◽  
Process Comparison ◽  
Central Composite Experimental Design ◽  
Oil Concentration

Pseudomonas aeruginosa was repeatedly reported as powerful producer of rhamnolipid biosurfactants as well as producer of hydrolytic enzymes. In this study effects of four fermentation factors were evaluated using response surface methodology and experiments were performed in accordance with a four-factor and five-level central composite experimental design. Investigated factors were: fermentation temperature, time of fermentation, concentration of sunflower oil and concentration of Tween? 80. The most important finding was that regression coefficients of the highest values were those that describe interactions between factors and that they differ for lipase and rhamnolipid production, which were both investigated in this study. Production of both metabolites was optimized and response equations were obtained, making it possible to predict rhamnolipid concentration or lipase activity from known values of the four factors. The highest achieved rhamnolipid concentration and lipase activity were 138 mg dm-3 (sunflower oil concentration 0.8 %, Tween? 80 concentration 0.05 %, temperature 30?C, and fermentation time 72 h) and 11111 IU dm-3(sunflower concentration of 0.4 %, Tween? 80 concentration of 0.05 %, temperature of 30?C, and fermentation time of 120 h), respectively.

scholarly journals Aktivitas Antibakteri Ekstrak Dan Fraksi Herba Kemangi (Ocimum Sanctum L) Terhadap Staphylococcus Aureus Dan Pseudomonas Aeruginosa

2019 ◽  
Vol 16 (2) ◽  
pp. 90-97
Author(s):  
Taufik Turahman
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Tween 80 ◽  
Ocimum Sanctum

Penyakit infeksi masih menjadi salah satu masalah kesehatan masyarakat khususnya di negara berkembang. Etiologi secara umum disebabkan oleh Staphylococcus aureus dan Pseudomonas aeruginosa. Herba kemangi (Ocimum sanctum L) merupakan tanaman yang dikenal sebagai lalapan ini memiliki khasiat sebagai antioksidan, antikanker dan antimikroba namun belum banyak informasi ilmiah sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui kandungan kimia dan aktivitas antibakteri dari ekstrak etanol, fraksi n-heksan, etil asetat dan air Herba kemangi terhadap pertumbuhan Staphylococcus aureus dan Pseudomonas aeruginosa secara difusi dan dilusi. Herba kemangi maserasi menggunakan etanol 96%. Ekstrak yang diperoleh kemudian disuspensi dengan air selanjutnya difraksinasi dengan n-heksan dan etil asetat. Ekstrak Herba kemangi beserta fraksi yang didapatkan selanjutnya diuji aktivitas antibakterinya dengan konsentrasi 12,5%, 25% dan 50% menggunakan metode difusi. KHM dan KBM dari fraksi teraktif bisa diketahui menggunakan uji dilusi dengan konsentrasi 0,78, 1,56, 3,125, 6,25%, 12,5%, 25% dan 50%. Kontrol positif ciprofloksasin 5μg/15μl dan kontrol negatif menggunakan tween 80 5% steril. Identifikasi golongan senyawa dilakukan dengan  metode uji tabung. Hasil uji aktifitas antibakteri menunjukan diameter zona hambatan pada bakteri gram negatif secara umum cendrung lebih besar dibandingkan bakteri gram positif. Diameter zona hambat terbesar pada bakteri Pseudomonas aeruginosa pada fraksi etil asetat Herba kemangi konsentrasi 50% dengan diameter zona hambat (10 mm), pada bakteri Staphylococcus aureus terdapat pada fraksi etil asetat pada konsentrasi 50 % memiliki daya hambat sebesar (8,6 mm). Hasil identifikasi kandungan kimia menunjukkan bahwa dalam ekstrak dan fraksi herba kemangi mengandung senyawa flavonoid, saponin dan steroid dan tanin   Kata kunci: Antibakteri, Herba kemangi (Ocimum sanctum L), Staphylococcus aureus, Pseudomonas aeruginosa

scholarly journals Review Analisis Teknologi Degradasi Limbah Minyak Bumi untuk Mengurangi Pencemaran Air Laut di Indonesia

2018 ◽  
Vol 3 (2) ◽  
pp. 80 ◽  
Author(s):  
Iffat Ganjar Fadhila Prakasita ◽  
Ria Wulansarie
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nonionic Surfactant ◽  
Oil Spills ◽  
Tween 80 ◽  
Chemical Degradation ◽  
Biological Degradation ◽  
Bacterial Concentration ◽  
Brij 35 ◽  
The Stability ◽  
Petroleum Emulsion

Penentuan metode degradasi minyak bumi yang tepat sangat diperlukan untuk mengatasi pencemaran tumpahan minyak bumi khususnya di air laut Indonesia. Metode degradasi limbah minyak bumi yang digunakan adalah metode degradasi secara kimia dan biologi yaitu menggunakan surfaktan nonionik (Tween 80 dan Brij 35) dan bioremediasi menggunakan bakteri Pseudomonas aeruginosa.Variabel yang digunakan pada metode degradasi kimia dengan surfaktan nonionik adalah variasi konsentrasi 0.0000, 0.0025, 0.0050, 0.0075, 0.0100, 0.0125, 0.0150, 0.0175, 0.0200, 0.0225, 0.0250, 0.0275, 0.0300, 0.0350, 0.0400 % (v/v) dan variasi pengadukan untuk menguji stabilitas emulsi minyak bumi dengan laju 100, 120, dan 140 rpm selama 1 jam. Variabel yang digunakan pada metode bioremediasi adalah konsentrasi penambahan mikroba Pseudomonas aeruginosa sebanyak 0%; 1%; 3% (v/v), konsentrasi cemaran minyak bumi 1000 ppm dan 1500 ppm serta media aerasi dan media tanpa aerasi. Hasil degradasi minyak bumi terbaik didapatkan pada metode bioremeidasi menggunakan Pseudomonas aeruginosa dengan konsentrasi bakteri sebanyak 3% (v/v) pada media teraerasi dan konsentrasi cemaran minyak 1000 ppm dengan % biodegradasi TPH yang dicapai sebesar 100% dalam waktu 21 hari.ABSTRACTDetermination of appropriate petroleum degradation method is needed to overcome the contamination of oil spills, especially in Indonesia's seawater. The method of degradation of petroleum waste used is chemical and biological degradation method using nonionic surfactant (Tween 80 and Brij 35) and bioremediation using Pseudomonas aeruginosa bacteria. Variable used in chemical degradation method with nonionic surfactant is concentration variation 0.0000; 0.0025; 0.0050; 0.0075; 0.0100; 0.0125; 0.0150; 0.0175; 0.0200; 0.0225; 0.0250; 0.0275; 0.0300; 0.0350, 0.0400% (v / v) and stirring variations to test the stability of petroleum emulsion at rates 100, 120, and 140 rpm for 1 hour. The variable used in the bioremediation method is the concentration of Pseudomonas aeruginosa addition of 0%; 1%; 3% (v/v), the concentration of petroleum contamination 1000 ppm and 1500 ppm also aeration medium and aeration non medium. The best petroleum degradation results were obtained in bioremediation method using Pseudomonas aeruginosa with bacterial concentration of 3% (v / v) in aerated media and concentration of 1000 ppm oil contamination with TPH biodegradation achieved at 100% within 21 days.

scholarly journals Avaliação dos efeitos de biossurfactantes produzidos por Bacillus subtilis LBBMA 4914 e Pseudomonas aeruginosa LBBMA 4951E e surfactantes sintéticos sobre larvas de Culex quinquefaciatus

2016 ◽  
Vol 7 (2) ◽  
pp. 101-110
Author(s):  
Adriano Guimaraes Parreira ◽  
Stenio Nunes Alves ◽  
Marcos Rogerio Totola
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Tween 80 ◽  
Triton X

Mosquitos da família Culicidae estão presentes em todo o território nacional podendo agir como vetores de doenças diversas, a exemplo da filariose. transmitida por Culex quinquefasciatus. A evidência de resistência aos inseticidas tradicionais tem estimulado o desenvolvimento de pesquisas com novos compostos com potencial ação larvicida. No presente trabalho foram avaliados os efeitos de agentes tensoativos biológicos, os biosurfactantes ramnolipideos produzidos por Pseudomonas aeruginosa LBBMA 4951e lipopetídeos produzidos por Bacillus subtilis LBBMA 4914, além dos sintéticos SDS - Dodecil Sulfato de Sódio, Twen 80 e Triton X, sobre larvas de 3 e 4 instar de C. quinquefasciatus. Os resultados obtidos mostraram-se bastantes promissores indicando grande potencial de ação bioinseticida por parte dos biossurfactantes avaliados, destacando-se a surfactina com ação larvicida sobre 75% do total de espécimes de C. quinquefascitus expostos àquele tensoativo ao longo de 96h. Em relação aos surfactantes sintéticos, observou-se resultado mais pronunciado para Triton X 100, seguido de Tween 80 e SDS, com valores de CL50 de 1,32x10-4M, 2,52x10-4M e 2,84x10-4M, respectivamente. Com isso, a partir deste trabalho abrem-se novas perspectivas para o uso daqueles tensoativos no controle da proliferação de C. quinquefascitus, atuando como bioinseticidas ou aditivos aos já utilizados, assim como para novos testes envolvendo a avaliação de seu efeito sobre outras espécies de Culicidae, a exemplo de Aedes aegypti.

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