The effect of Polysorbate (Tween) 80 on the growth rate of Pseudomonas aeruginosa

1964 ◽  
Vol 16 (S1) ◽  
pp. 41T-45T ◽  
Author(s):  
M. R. W. BROWN ◽  
R. M. E. RICHARDS
Keyword(s):  
Growth Rate ◽  
Pseudomonas Aeruginosa ◽  
Tween 80

scholarly journals Factors Influencing the Chlorine Susceptibility of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum

2003 ◽  
Vol 69 (9) ◽  
pp. 5685-5689 ◽  
Author(s):  
Joseph O. Falkinham
Keyword(s):  
Growth Rate ◽  
Mycobacterium Avium ◽  
Growth Phase ◽  
Growth Rates ◽  
Growth Stage ◽  
Tween 80 ◽  
Culture Conditions ◽  
C Cells ◽  
Mycobacterium Intracellulare ◽  
Factors Influencing

ABSTRACT The susceptibility of representative strains of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (the MAIS group) to chlorine was studied to identify factors related to culture conditions and growth phase that influenced susceptibility. M. avium and M. intracellulare strains were more resistant to chlorine than were strains of M. scrofulaceum. Transparent and unpigmented colony variants were more resistant to chlorine than were their isogenic opaque and pigmented variants (respectively). Depending on growth stage and growth rate, MAIS strains differed in their chlorine susceptibilities. Cells from strains of all three species growing in early log phase at the highest growth rates were more susceptible than cells in log and stationary phase. Rapidly growing cells were more susceptible to chlorine than slowly growing cells. The chlorine susceptibility of M. avium cells grown at 30°C was increased when cells were exposed to chlorine at 40°C compared to susceptibility after exposure at 30°C. Cells of M. avium grown in 6% oxygen were significantly more chlorine susceptible than cells grown in air. Chlorine-resistant MAIS strains were more hydrophobic and resistant to Tween 80, para-nitrobenzoate, hydroxylamine, and nitrite than were the chlorine-sensitive strains.

scholarly journals STUDI PERBANDINGAN BIOSURFAKTAN Pseudomonas aeruginosa IA7d DAN SURFAKTAN SINTETIK Tween-80 DALAM BIODEGRADASI SOLAR OLEH MIKROBA PERAIRAN PELABUHAN TANJUNG PERAK SURABAYA

2006 ◽  
Vol 12 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Ni’matuzahroh Ni’matuzahroh ◽  
Arif Yachya ◽  
Mulyadi Tanjung
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diesel Fuel ◽  
Room Temperature ◽  
Surfactant Solution ◽  
Tween 80 ◽  
Synthetic Surfactant ◽  
Microbial Count ◽  
Crude Product ◽  
And Control ◽  
Biodegradation Test

This research aimed to know the influence of addition crude product Pseudomonas aeruginosa IA7d biosurfactant from substrate hexadecane and diesel fuel in supporting activity of diesel fuel biodegradation and both potention to replace synthetic surfactant for hydrocarbon remediation. Biodegradation test was done by experimental in laboratory scale by Tween-80 as synthetic surfactant comparator. Growth culture was from seawater of Tanjung Perak Harbor Surabaya include various microbe types in its. 2 percent (w/v) of diesel fuel and three surfactants test (0.70 percent w/v crude product P. aeruginosa IA7d biosurfactant from hexadecane substrate; 60,69 percent w/v crude product P. aeruginosa IA7d biosurfactant from diesel fuel substrate; and 0.10 percent w/v synthetic surfactant Tween 80) at uniform surface tension of surfactant solution (54.03 mN/m) were added into 30 ml seawater sample. Cultures were incubated at room temperature (30 °C) during 14 days with shaking (90 rpm). Result of biodegradation test showed the third addition of surfactants test had an effect on acceleration of diesel fuel biodegradation activity that proved at the increased of microbial count (bacterium, yeast, and mould) and decreased of diesel fuel concentration was shown by chromatograms area of diesel fuel. Highest degradation was successively reached by culture got crude product P. aeruginosa IA7d biosurfactant from diesel fuel substrate (88.034 percent), crude product P. aeruginosa IA7d biosurfactant from hexadecane substrate (80.908 percent), synthetic surfactant Tween-80 (54.732 percent), and control that was without addition surfactant test (0.132 percent). Base on number of microbial count, percentage of decreasing chromatogram peaks of diesel fuel from each surfactant test, and price substrate produce (diesel fuel and hexadecane) among both crude product P. aeruginosa IA7d biosurfactant, known that the crude product P. aeruginosa IA7d biosurfactant from diesel fuel substrate had highest ability acceleration to biodegradation activity on diesel fuel and had a potency to replace synthetic surfactant for remediation hydrocarbon application in environment.

scholarly journals Rhamnolipid and lipase production by Pseudomonas aeruginosa san-ai: the process comparison analysis by statistical approach

2013 ◽  
Vol 67 (4) ◽  
pp. 677-685 ◽  
Author(s):  
Sonja Jakovetic ◽  
Zorica Knezevic-Jugovic ◽  
Sanja Grbavcic ◽  
Dejan Bezbradica ◽  
Natasa Avramovic ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Lipase Activity ◽  
Sunflower Oil ◽  
Hydrolytic Enzymes ◽  
Tween 80 ◽  
Lipase Production ◽  
Fermentation Time ◽  
Process Comparison ◽  
Central Composite Experimental Design ◽  
Oil Concentration

Pseudomonas aeruginosa was repeatedly reported as powerful producer of rhamnolipid biosurfactants as well as producer of hydrolytic enzymes. In this study effects of four fermentation factors were evaluated using response surface methodology and experiments were performed in accordance with a four-factor and five-level central composite experimental design. Investigated factors were: fermentation temperature, time of fermentation, concentration of sunflower oil and concentration of Tween? 80. The most important finding was that regression coefficients of the highest values were those that describe interactions between factors and that they differ for lipase and rhamnolipid production, which were both investigated in this study. Production of both metabolites was optimized and response equations were obtained, making it possible to predict rhamnolipid concentration or lipase activity from known values of the four factors. The highest achieved rhamnolipid concentration and lipase activity were 138 mg dm-3 (sunflower oil concentration 0.8 %, Tween? 80 concentration 0.05 %, temperature 30?C, and fermentation time 72 h) and 11111 IU dm-3(sunflower concentration of 0.4 %, Tween? 80 concentration of 0.05 %, temperature of 30?C, and fermentation time of 120 h), respectively.

scholarly journals Aktivitas Antibakteri Ekstrak Dan Fraksi Herba Kemangi (Ocimum Sanctum L) Terhadap Staphylococcus Aureus Dan Pseudomonas Aeruginosa

2019 ◽  
Vol 16 (2) ◽  
pp. 90-97
Author(s):  
Taufik Turahman
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Tween 80 ◽  
Ocimum Sanctum

Penyakit infeksi masih menjadi salah satu masalah kesehatan masyarakat khususnya di negara berkembang. Etiologi secara umum disebabkan oleh Staphylococcus aureus dan Pseudomonas aeruginosa. Herba kemangi (Ocimum sanctum L) merupakan tanaman yang dikenal sebagai lalapan ini memiliki khasiat sebagai antioksidan, antikanker dan antimikroba namun belum banyak informasi ilmiah sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui kandungan kimia dan aktivitas antibakteri dari ekstrak etanol, fraksi n-heksan, etil asetat dan air Herba kemangi terhadap pertumbuhan Staphylococcus aureus dan Pseudomonas aeruginosa secara difusi dan dilusi. Herba kemangi maserasi menggunakan etanol 96%. Ekstrak yang diperoleh kemudian disuspensi dengan air selanjutnya difraksinasi dengan n-heksan dan etil asetat. Ekstrak Herba kemangi beserta fraksi yang didapatkan selanjutnya diuji aktivitas antibakterinya dengan konsentrasi 12,5%, 25% dan 50% menggunakan metode difusi. KHM dan KBM dari fraksi teraktif bisa diketahui menggunakan uji dilusi dengan konsentrasi 0,78, 1,56, 3,125, 6,25%, 12,5%, 25% dan 50%. Kontrol positif ciprofloksasin 5μg/15μl dan kontrol negatif menggunakan tween 80 5% steril. Identifikasi golongan senyawa dilakukan dengan  metode uji tabung. Hasil uji aktifitas antibakteri menunjukan diameter zona hambatan pada bakteri gram negatif secara umum cendrung lebih besar dibandingkan bakteri gram positif. Diameter zona hambat terbesar pada bakteri Pseudomonas aeruginosa pada fraksi etil asetat Herba kemangi konsentrasi 50% dengan diameter zona hambat (10 mm), pada bakteri Staphylococcus aureus terdapat pada fraksi etil asetat pada konsentrasi 50 % memiliki daya hambat sebesar (8,6 mm). Hasil identifikasi kandungan kimia menunjukkan bahwa dalam ekstrak dan fraksi herba kemangi mengandung senyawa flavonoid, saponin dan steroid dan tanin   Kata kunci: Antibakteri, Herba kemangi (Ocimum sanctum L), Staphylococcus aureus, Pseudomonas aeruginosa

scholarly journals Influence of a Putative ECF Sigma Factor on Expression of the Major Outer Membrane Protein, OprF, in Pseudomonas aeruginosa and Pseudomonas fluorescens

1999 ◽  
Vol 181 (16) ◽  
pp. 4746-4754 ◽  
Author(s):  
Fiona S. L. Brinkman ◽  
Geert Schoofs ◽  
Robert E. W. Hancock ◽  
René De Mot
Keyword(s):  
Growth Rate ◽  
Pseudomonas Aeruginosa ◽  
Membrane Protein ◽  
Outer Membrane Protein ◽  
Sigma Factor ◽  
Logarithmic Phase ◽  
Rich Medium ◽  
Phase Growth ◽  
Ecf Sigma Factor ◽  
Sigma 70

ABSTRACT The gene encoding OprF, a major outer membrane protein inPseudomonas species (formerly known as type 1 pseudomonads), was thought to be constitutively transcribed from a single sigma 70 promoter immediately upstream of the gene. We now report the identification of a novel putative ECF (extracytoplasmic function) sigma factor gene, sigX, located immediately upstream of oprF in both Pseudomonas aeruginosaPAO1 and Pseudomonas fluorescens OE 28.3 and show that disruption of this gene significantly reduces OprF expression. InP. aeruginosa, Northern analysis demonstrated that this reduction was a result of an effect on transcription of monocistronicoprF combined with a polar effect due to termination of a transcript containing sigX and oprF. Comparison of sigX-disrupted and wild-type cell transcripts by primer extension indicated that monocistronic transcription ofoprF occurs from two overlapping promoters, one that is SigX-dependent and resembles ECF sigma factor promoters in its minus-35 region and another promoter that is independent of SigX and is analogous to the sigma 70-type promoter previously reported. Complementation of the P. aeruginosa sigX-disrupted mutant with plasmid-encoded OprF did not resolve the phenotypes associated with this mutant, which included a markedly reduced logarithmic-phase growth rate in rich medium (compared to that in minimal medium), further reduction of the growth rate in a low-osmolarity environment, secretion of an unidentified pigment, and increased sensitivity to the antibiotic imipenem. This indicates that SigX is involved in the regulation of other genes in P. aeruginosa. Disruption of the sigX gene in P. fluorescens also had an effect on the logarithmic-phase growth rate in rich medium. A conservedsigX gene was also identified in a Pseudomonas syringae isolate and six P. aeruginosa clinical isolates. Collectively, these data indicate that an ECF sigma factor plays a role in the regulation and expression of OprF and also affects other genes.

Dietary Effect of Padina boergesenii on Growth, Immune Response and Disease Resistance against Pseudomonas aeruginosa in Cirrhinus mrigala.

2021 ◽  
Author(s):  
Ragunath C ◽  
Ramasubramanian C
Keyword(s):  
Mass Spectrometry ◽  
Growth Rate ◽  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Renewable Resources ◽  
Specific Growth ◽  
Growth Parameters ◽  
Basal Diet ◽  
Antibiotic Activity ◽  
Gas Chromatography Mass Spectrometry

Abstract Seaweeds are potential renewable resources in the aquatic environment. The present study was made to evaluate the influence of seaweed Padina boergesenii incorporated with basal diet in different concentrations. The Phyto-constituents of the seaweeds were characterized by Gas Chromatography Mass Spectrometry. Diets were formulated to include seaweed meal at elevated levels from 0.5%, 2.5%, 4.5% and 6.5%. The C. mrigala fed with P.boergesenii incorporated with basal diet for a period of 45 days. The Growth parameters (weight gain, Specific growth rate) and biochemical constituents were observed significantly. The fraction of extracts showed effective inhibitory activity against Pseudomonas aeruginosa. Hence, this study suggests that extracts of seaweed P. boergesenii, contain potential bioactive compounds with considerable antibiotic activity.

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