Isolation and identification of Legionella spp. from hot spring water in Algeria by culture and molecular methods

Author(s):  
N. Boilattabi ◽  
L. Barrassi ◽  
A. Bouanane‐Darenfed ◽  
B. La Scola
Author(s):  
Eka Djatnika Nugraha ◽  
Masahiro Hosoda ◽  
June Mellawati ◽  
Untara Untara ◽  
Ilsa Rosianna ◽  
...  

The world community has long used natural hot springs for tourist and medicinal purposes. In Indonesia, the province of West Java, which is naturally surrounded by volcanoes, is the main destination for hot spring tourism. This paper is the first report on radon measurements in tourism natural hot spring water in Indonesia as part of radiation protection for public health. The purpose of this paper is to study the contribution of radon doses from natural hot spring water and thereby facilitate radiation protection for public health. A total of 18 water samples were measured with an electrostatic collection type radon monitor (RAD7, Durridge Co., USA). The concentration of radon in natural hot spring water samples in the West Java region, Indonesia ranges from 0.26 to 31 Bq L−1. An estimate of the annual effective dose in the natural hot spring water area ranges from 0.51 to 0.71 mSv with a mean of 0.60 mSv for workers. Meanwhile, the annual effective dose for the public ranges from 0.10 to 0.14 mSv with an average of 0.12 mSv. This value is within the range of the average committed effective dose from inhalation and terrestrial radiation for the general public, 1.7 mSv annually.


Author(s):  
Teuku Zahrial Helmi ◽  
Charles Rangga Tabbu ◽  
Wayan Tunas Artama ◽  
Aris Haryanto ◽  
Muhammad Isa

The purpose of this research was to identify avian influenza (AI) virus using serological and molecular methods on poultry which suspectedas AI infected in Aceh province. This study used 37 samples of tracheal and cloacal swabs and organs from various species of poultry that werecollected from several districts/cities in Aceh. Samples were collected and put into transport media and stored at 4° C before sending to thelaboratory. Samples were inoculated in specific pathogen-free of embryonated chicken egg with the age of 9-11 days for further serological andmolecular examination. From 37 samples which infected to embryonated chicken egg then followed by hemagglutinin agglutinationtest/hemagglutinin inhibition revealed that 7 samples were positively infected with AI virus. The amplification result of specific matrix geneprimer was followed by electrophoresis on 2% agarose gel which were obtained in the form of a deoxyribonucleic acid (DNA) band at 276 bp formatrix gene and 1.725 bp for H5 gene for all isolates test. In conclusion, the virus which caused the death of various types of poultry in Acehprovince is avian influenza A virus subtype H5.Key words: avian influenza virus, H5N1, serologic, matrix, heamaglutinin 


Author(s):  
Sutthipong Taweelarp ◽  
Supanut Suntikoon ◽  
Thaned Rojsiraphisal ◽  
Nattapol Ploymaklam ◽  
Schradh Saenton

Scaling in a geothermal piping system can cause serious problems by reducing flow rates and energy efficiency. In this work, scaling potential of San Kamphaeng (SK) geothermal energy, Northern Thailand was assessed based on geochemical model simulation using physical and chemical properties of hot spring water. Water samples from surface seepage and groundwater wells, analyzed by ICP-OES and ion chromatograph methods for chemical constituents, were dominated by Ca-HCO3 facies having partial pressure of carbon dioxide of 10–2.67 to 10–1.75 atm which is higher than ambient atmospheric CO2 content. Surface seepage samples have lower temperature (60.9°C) than deep groundwater (83.1°C) and reservoir (127.1°C, based on silica geothermometry). Geochemical characteristics of the hot spring water indicated significant difference in chemical properties between surface seepage and deep, hot groundwater as a result of mineral precipitation along the flow paths and inside well casing. Scales were mainly composed of carbonates, silica, Fe-Mn oxides. Geochemical simulations based on multiple chemical reaction equilibria in PHREEQC were performed to confirm scale formation from cooling and CO2-degassing processes. Simulation results showed total cumulative scaling potential (maximum possible precipitation) from 267-m deep well was estimated as 582.2 mg/L, but only 50.4% of scaling potential actually took place at SK hot springs. In addition, maximum possible carbon dioxide outflux to atmosphere from degassing process in SK geothermal field, estimated from the degassing process, was 6,960 ton/year indicating a continuous source of greenhouse gas that may contribute to climate change. Keywords: Degassing, Geochemical modeling, PHREEQC, San Kamphaeng Hot Springs, Scaling


2014 ◽  
Vol 2 (12) ◽  
pp. 7293-7308
Author(s):  
Z. Chen ◽  
X. Zhou ◽  
J. Du ◽  
C. Xie ◽  
L. Liu ◽  
...  

Abstract. Hydrogeochemistry of 10 hot springs in the Kangding district was investigated by analyzing cation and anion concentrations of the spring waters. The water samples were collected within 5 days after the Lushan earthquake. The spring waters are classified into 7 chemical types based on the hydrochemical compositions. Comparison with the hydrochemical data before the Lushan earthquake, concentrations of Ca2+, HCO3− and TDS of the waters from the Guanding, Erdaoqiao, Gonghe, Erhaoying, Tianwanhe and Caoke springs evidently increased, which resulted from enhancing interaction between deep-earth fluids and carbonate rocks by the increment of dissolved CO2 in the groundwater. Concentrations of Na+, Cl− and SO42− of the waters from the Guanding, zheduotang, Xinxing and Gonghe springs were decreased, indicating dilution of precipitation water. Concentrations of Na+ and SO42− of the Erhaoying spring water increased, which may be attributed to the more supplement of fluids enriched in sulfur. The results indicate that hydrochemical components of spring water can be used as an effective indicator for earthquakes.


Life ◽  
2018 ◽  
Vol 8 (2) ◽  
pp. 11 ◽  
Author(s):  
Daniel Milshteyn ◽  
Bruce Damer ◽  
Jeff Havig ◽  
David Deamer

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Takuya Okazaki ◽  
Senshin Umeki ◽  
Tatsuya Orii ◽  
Ryusuke Ikeya ◽  
Aya Sakaguchi ◽  
...  

Author(s):  
Wassila Boukhenfouf ◽  
Ahmed Boucenna

Abstract A radiological analysis of hot spring water of Hammam Debagh (also called Hammam Meskhoutine) and its associated deposits was realized. Hammam Debagh is considered to be the second hottest springs in the world with a temperature of 98°C and this lead to the creation of travertine. This later was formed because of limestone deposited by mineral springs in a rapid sedimentation of calcium, magnesium bicarbonates, and sulphides. The analysis showed that two-thirds of radioactivity was left by spring water in their deposits. The presence of uranium in the spring deposits was due to the property of the uranyl ion to form stable complexes with carbonates and precipitates. The radiation hazards indexes were estimated and compared with those given in literature.


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