scholarly journals Synergistic antimicrobial activities of epigallocatechin gallate, myricetin, daidzein, gallic acid, epicatechin, 3‐hydroxy‐6‐methoxyflavone and genistein combined with antibiotics against ESKAPE pathogens

2021 ◽  
Author(s):  
David Buchmann ◽  
Nadin Schultze ◽  
Julia Borchardt ◽  
Inken Böttcher ◽  
Katharina Schaufler ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Epigallocatechin Gallate ◽  
Antimicrobial Activities ◽  
Eskape Pathogens

scholarly journals Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Helena Abramovič ◽  
Blaž Grobin ◽  
Nataša Poklar Ulrih ◽  
Blaž Cigić
Keyword(s):  
Ascorbic Acid ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Sulfonic Acid ◽  
Epigallocatechin Gallate ◽  
The Other ◽  
Oh Groups ◽  
Standard Compound

Trolox, gallic acid, chlorogenic acid, caffeic acid, catechin, epigallocatechin gallate, and ascorbic acid are antioxidants used as standards for reaction with chromogenic radicals, 2,2-diphenyl-1-picrylhydrazyl (DPPH⋅) and 2,2′-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS⋅+), and Folin–Ciocalteu (FC) reagent. The number of exchanged electrons has been analyzed as function of method and solvent. A majority of compounds exchange more electrons in FC assay than in ABTS and DPPH assays. In reaction with chromogenic radicals, the largest number of electrons was exchanged in buffer (pH 7.4) and the lowest reactivity was in methanol (DPPH) and water (ABTS). At physiological pH, the number of exchanged electrons of polyphenols exceeded the number of OH groups, pointing to the important contribution of partially oxidized antioxidants, formed in the course of reaction, to the antioxidant potential. For Trolox, small impact on the number of exchanged electrons was observed, confirming that it is more suitable as a standard compound than the other antioxidants.

scholarly journals Aldose Reductase Differential Inhibitors in Green Tea

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1003 ◽  
Author(s):  
Francesco Balestri ◽  
Giulio Poli ◽  
Carlotta Pineschi ◽  
Roberta Moschini ◽  
Mario Cappiello ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Green Tea ◽  
Aldose Reductase ◽  
Active Site ◽  
Inhibitory Action ◽  
Computational Study ◽  
Epigallocatechin Gallate ◽  
Polyol Pathway ◽  
Differential Inhibition ◽  
Tea Extract

Aldose reductase (AKR1B1), the first enzyme in the polyol pathway, is likely involved in the onset of diabetic complications. Differential inhibition of AKR1B1 has been proposed to counteract the damaging effects linked to the activity of the enzyme while preserving its detoxifying ability. Here, we show that epigallocatechin gallate (EGCG), one of the most representative catechins present in green tea, acts as a differential inhibitor of human recombinant AKR1B1. A kinetic analysis of EGCG, and of its components, gallic acid (GA) and epigallocatechin (EGC) as inhibitors of the reduction of L-idose, 4-hydroxy2,3-nonenal (HNE), and 3-glutathionyl l-4-dihydroxynonanal (GSHNE) revealed for the compounds a different model of inhibition toward the different substrates. While EGCG preferentially inhibited L-idose and GSHNE reduction with respect to HNE, gallic acid, which was still active in inhibiting the reduction of the sugar, was less active in inhibiting HNE and GSHNE reduction. EGC was found to be less efficient as an inhibitor of AKR1B1 and devoid of any differential inhibitory action. A computational study defined different interactive modes for the three substrates on the AKR1B1 active site and suggested a rationale for the observed differential inhibition. A chromatographic fractionation of an alcoholic green tea extract revealed that, besides EGCG and GA, other components may exhibit the differential inhibition of AKR1B1.

High-Added-Value Antioxidants Obtained from the Degradation of Wine Phenolics by Lactobacillus plantarum

2007 ◽  
Vol 70 (11) ◽  
pp. 2670-2675 ◽  
Author(s):  
JOSÉ MARÍA LANDETE ◽  
HÉCTOR RODRÍGUEZ ◽  
BLANCA DE LAS RIVAS ◽  
ROSARIO MUÑOZ
Keyword(s):  
Phenolic Compounds ◽  
Gallic Acid ◽  
Lactobacillus Plantarum ◽  
Antimicrobial Activities ◽  
Added Value ◽  
Methyl Gallate ◽  
Biotechnological Production ◽  
Wine Production ◽  
High Concentrations ◽  
Ferulic Acids

Disposal of the waste from wine production has long been a problem for wineries, mainly because of the presence of phenolic compounds. In this study, we analyzed the antimicrobial activities of 10 wine phenolic compounds against Lactobacillus plantarum strains. Inhibition increased in this order: catechin = gallic acid < epicatechin = salicylic acid < methyl gallate = caffeic acid < ferulic acid = tryptophol < p-coumaric acid. The obtained results indicated that L. plantarum is able to grow in the presence of high concentrations of some wine phenolic compounds. Of the 10 compounds analyzed, only the hydroxycinnamic acids, gallic acid, and methyl gallate were metabolized by the four L. plantarum strains studied. Results also revealed that 4-vinylphenol and 4-vinylguaiacol are originated from p-coumaric and ferulic acids. These phenolic compounds are valuable intermediates in the biotechnological production of new fragrances. In addition, gallic acid and its ester, methyl gallate, are metabolized to produce the powerful antioxidant pyrogallol. Therefore, it might be possible to use L. plantarum strains to obtain high-added-value antioxidants from the degradation of phenolic compounds found in wine wastes.

Study of the release of gallic acid from (-)-epigallocatechin gallate in old oolong tea by mass spectrometry

2010 ◽  
Vol 24 (7) ◽  
pp. 851-858 ◽  
Author(s):  
Ren-Jye Lee ◽  
Viola S. Y. Lee ◽  
Jason T. C. Tzen ◽  
Maw-Rong Lee
Keyword(s):  
Mass Spectrometry ◽  
Gallic Acid ◽  
Epigallocatechin Gallate ◽  
Oolong Tea

scholarly journals Evaluation of anticancer and antimicrobial activities of the Polygonum maritimum ethanol extract

2018 ◽  
Vol 70 (4) ◽  
pp. 665-673 ◽  
Author(s):  
Marina Jovanovic ◽  
Tatjana Srdic-Rajic ◽  
Emilija Svircev ◽  
Nebojsa Jasnic ◽  
Biljana Nikolic ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Viability ◽  
Herbal Remedy ◽  
Antimicrobial Properties ◽  
Epigallocatechin Gallate ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Cell Cycle Distribution ◽  
M Cell ◽  
Ic50 Values

Polygonum maritimum is a traditional herbal remedy that produces abundant flavonoid secondary metabolites. The ethanol extract of P. maritimum aerial parts (POM) was chemically characterized and tested for antimicrobial properties and cytotoxicity. Results of LC-MS/MS analysis showed high contents of gallic acid, epigallocatechin gallate and catechin, and significant amounts of quercetin-3-O-galactoside and quercetin-3-O-glucoside. Evaluation of the antifungal properties revealed that POM induced notable growth inhibition of Alternaria alternata (34.3%), Penicillium spp. (30.6%), Fusarium semitectum (20.2%) and Aspergillus spp. (19.6%). Evaluation of cytotoxicity against human hepatoma HepG2 cells included monitoring the effects of both POM alone and its combination with cytostatic doxorubicin (Dox). Cell viability, apoptosis and cell cycle distribution and the expression of antioxidant enzymes (superoxide-dismutases SOD1 and SOD2 and catalase) were determined. A dose-dependent decrease in cell viability was detected, but a remarkably stronger effect was obtained when POM and Dox were applied in combination as compared to individual treatments. IC50 values were determined to be 393 ?g/mL (POM) and 2.24 ?g/mL (Dox) in combination, but 1153 ?g/mL (POM) and 12.56 ?g/mL (Dox) in a single treatment. The value of the Loewe index, determined for IC50, was notably lower than 1 (LI=0.51), clearly indicating synergism of POM and Dox. Additionally, POM and POM +Dox induced early/late apoptosis and G2/M cell cycle arrest. Furthermore, POM increased, while Dox decreased the expression levels of SODs and catalase. The obtained results encourage further examination of the potential use of POM in modern phytotherapy.

Bergenin – a biologically active scaffold: Nanotechnological perspectives

2021 ◽  
Vol 21 ◽  
Author(s):  
Reecha Madaan ◽  
Rajeev K. Singla ◽  
Suresh Kumar ◽  
Ankit Kumar Dubey ◽  
Dinesh Kumar ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Gallic Acid ◽  
Therapeutic Agent ◽  
Bioactive Compound ◽  
Antimicrobial Activities ◽  
Docking Studies ◽  
Biologically Active ◽  
Google Scholar ◽  
Activity Relationship ◽  
Structure Activity

: Bergenin, 4-O-methyl gallic acid glucoside, is a bioactive compound present in various plants belonging to different families. The present work compiles scattered information on pharmacology, structure activity relationship and nanotechnological aspects of bergenin, collected from various electronic databases such as Sci Finder, PubMed, Google scholar, etc. Bergenin has been reported to exhibit hepatoprotective, anti-inflammatory, anticancer, neuroprotective, antiviral and antimicrobial activities. Molecular docking studies have shown that isocoumarin pharmacophore of bergenin is essential for its bioactivities. Bergenin holds a great potential to be used as lead molecule and also as a therapeutic agent for development of more efficacious and safer semisynthetic derivatives. Nanotechnological concepts can be employed to overcome poor bioavailability of bergenin. Finally, it is concluded that bergenin can be emerged as clinically potential medicine in modern therapeutics.

scholarly journals Preparation and characterization of squid pen chitooligosaccharide–epigallocatechin gallate conjugates and their antioxidant and antimicrobial activities

RSC Advances ◽  
2020 ◽  
Vol 10 (55) ◽  
pp. 33196-33204
Author(s):  
Avtar Singh ◽  
Soottawat Benjakul ◽  
Nurul Huda ◽  
Changan Xu ◽  
Peng Wu
Keyword(s):  
Epigallocatechin Gallate ◽  
Antimicrobial Activities ◽  
Antioxidant And Antimicrobial Activities

Chitooligosaccharide from squid pen showed increases in both antioxidant and antimicrobial activities via conjugation with epigallocatechin-gallate (EGCG).

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