Deep‐learning system for real‐time differentiation between Crohn's disease, intestinal Behçet's disease, and intestinal tuberculosis

2021 ◽  
Author(s):  
Jung Min Kim ◽  
Jun Gu Kang ◽  
Sungwon Kim ◽  
Jae Hee Cheon
Keyword(s):  
Crohn’S Disease ◽  
Deep Learning ◽  
Crohn's Disease ◽  
Real Time ◽  
Behcet’S Disease ◽  
Intestinal Tuberculosis ◽  
Learning System ◽  
Intestinal Behçet’S Disease ◽  
Intestinal Behcet’S Disease ◽  
Intestinal Behçet's Disease

scholarly journals Proteomic analysis-based discovery of a novel biomarker that differentiates intestinal Behçet’s disease from Crohn’s disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jihye Park ◽  
Daeun Jeong ◽  
Youn Wook Chung ◽  
Seunghan Han ◽  
Da Hye Kim ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Proteomic Analysis ◽  
Behcet’S Disease ◽  
Differentially Expressed ◽  
Tandem Mass ◽  
Intestinal Behçet’S Disease ◽  
Tissue Samples ◽  
Intestinal Behcet’S Disease ◽  
Intestinal Behçet's Disease

AbstractIntestinal Behçet’s disease (BD) and Crohn’s disease (CD) present similar manifestations, but there are no specific diagnostic tests to differentiate them. We used a proteomic approach to discover novel diagnostic biomarkers specific to intestinal BD. Colon mucosa tissue samples were obtained from patients with intestinal BD or CD using colonoscopy-guided biopsy of the affected bowel. Peptides from seven intestinal BD and seven CD patients were extracted and labeled using tandem mass tag (TMT) reagents. The labeled peptides were identified and quantified using liquid chromatography-tandem mass spectrometry (LC–MS/MS). The proteins were further validated using immunohistochemical (IHC) analysis with tissue samples and an ELISA test with serum samples from 20 intestinal BD and 20 CD patients. Using TMT/LC–MS/MS-based proteomic quantification, we identified 39 proteins differentially expressed between intestinal BD and CD. Beta-2 glycoprotein 1 (APOH) and maltase-glucoamylase (MGAM) showed higher intensity in the IHC staining of intestinal BD tissues than in CD tissues. The serum MGAM level was higher in intestinal BD patients. Proteomic analysis revealed that some proteins were differentially expressed in patients with intestinal BD compared with those with CD. Differential MGAM expression in intestinal BD suggests its role as a potential novel diagnostic biomarker.

scholarly journals Management and outcomes of surgical patients with intestinal Behçet’s disease and Crohn’s disease in southwest China

2021 ◽  
Vol 9 (16) ◽  
pp. 3858-3868
Author(s):  
Li Zeng ◽  
Wen-Jian Meng ◽  
Zhong-Hui Wen ◽  
Yi-Long Chen ◽  
Yu-Fang Wang ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Behcet’S Disease ◽  
Southwest China ◽  
Behçet's Disease ◽  
Surgical Patients ◽  
Intestinal Behçet’S Disease ◽  
Behcet's Disease ◽  
Intestinal Behcet’S Disease ◽  
Intestinal Behçet's Disease

Worse prognosis of intestinal Behcet's disease in comparison with Crohn's disease and risk factor evaluation

2000 ◽  
Vol 118 (4) ◽  
pp. A582
Author(s):  
Hyun Chae Jung ◽  
Il Ju Choi ◽  
You Sun Kim ◽  
Min Jung Park ◽  
Joo Sung Kim ◽  
...  
Keyword(s):  
Risk Factor ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Behcet’S Disease ◽  
Behçet's Disease ◽  
Intestinal Behçet’S Disease ◽  
Factor Evaluation ◽  
Intestinal Behcet’S Disease ◽  
Worse Prognosis ◽  
Intestinal Behçet's Disease

scholarly journals P058 Proteomic analysis-based discovery of a novel biomarker that differentiates intestinal Behçet’s disease from Crohn’s disease

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S164-S165
Author(s):  
J Park ◽  
J Daeun ◽  
C Youn Wook ◽  
C Jae Hee ◽  
R Ji-Hwan
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Proteomic Analysis ◽  
Differentially Expressed ◽  
Tandem Mass ◽  
Diagnostic Biomarker ◽  
Intestinal Behçet’S Disease ◽  
Tissue Samples ◽  
Intestinal Behcet’S Disease ◽  
Intestinal Behçet's Disease

Abstract Background Intestinal Behçet’s disease (BD) and Crohn’s disease (CD) present similar manifestations, but there are no specific pathognomonic clinical, laboratory, or histological diagnostic tests to differentiate intestinal BD from CD. We used a proteomic approach to discover a novel diagnostic biomarker specific to intestinal BD. Methods The colon mucosa tissue samples were obtained using colonoscopy-guided biopsy of the affected bowel from patients with intestinal BD or CD at the Inflammatory Bowel Disease Clinic of Severance Hospital, Seoul, Korea. Peptides from seven intestinal BD and seven CD patients were extracted and labeled using tandem mass tag (TMT) reagents. The labeled peptides were identified and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The differentially expressed proteins were further validated using immunohistochemical (IHC) analysis with tissue samples and an ELISA test with serum samples from 20 intestinal BD and 20 CD patients. Results A total of 3,266 proteins were identified using TMT/LC-MS/MS-based proteomic quantification, including 39 candidate proteins differentially expressed between intestinal BD and CD. Beta-2 glycoprotein 1 (APOH) and maltase-glucoamylase (MGAM) showed significantly higher intensity in the IHC staining of the intestinal BD tissues than that of CD tissues. Furthermore, the serum MGAM level was significantly higher in patients with intestinal BD than in patients with CD. Conclusion Our proteomic analysis revealed that some proteins were differentially expressed in intestinal BD patients compared to CD patients. Differential MGAM expression in intestinal BD suggests its role as a potential novel diagnostic biomarker in the differentiation of intestinal BD from CD.

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