scholarly journals Automatic segmentation of fluorescence lifetime microscopy images of cells using multiresolution community detection-a first study

2013 ◽  
Vol 253 (1) ◽  
pp. 54-64 ◽  
Author(s):  
D. HU ◽  
P. SARDER ◽  
P. RONHOVDE ◽  
S. ORTHAUS ◽  
S. ACHILEFU ◽  
...  
2019 ◽  
Vol 25 (3) ◽  
pp. 711-719 ◽  
Author(s):  
Hanqing Zhang ◽  
Niklas Söderholm ◽  
Linda Sandblad ◽  
Krister Wiklund ◽  
Magnus Andersson

AbstractAnalysis of numerous filamentous structures in an image is often limited by the ability of algorithms to accurately segment complex structures or structures within a dense population. It is even more problematic if these structures continuously grow when recording a time-series of images. To overcome these issues we present DSeg; an image analysis program designed to process time-series image data, as well as single images, to segment filamentous structures. The program includes a robust binary level-set algorithm modified to use size constraints, edge intensity, and past information. We verify our algorithms using synthetic data, differential interference contrast images of filamentous prokaryotes, and transmission electron microscopy images of bacterial adhesion fimbriae. DSeg includes automatic segmentation, tools for analysis, and drift correction, and outputs statistical data such as persistence length, growth rate, and growth direction. The program is available at Sourceforge.


2014 ◽  
Vol 556-562 ◽  
pp. 4941-4944
Author(s):  
Li Xiang Shi ◽  
Li Peng ◽  
Lu Lu Yue ◽  
Zhi Xing Huang

We use deep max-pooling convolutional neural networks to address a problem of neuroanatomy, namely, the automatic segmentation of cerebral cortex structures of laboratory rat depicted in stacks of Two-photon microscopy images and detect the change areas when stimulation occurs. We classify each pixel in the image by training a CNN network, using a square window to predict the probability of the central pixel for each class. After classification, we perform the post-processing on the output produced by CNN. At last, we depict the areas that we interested through a threshold value.


2013 ◽  
Vol 60 (3) ◽  
pp. 803-812 ◽  
Author(s):  
C. T. N. Suzuki ◽  
J. F. Gomes ◽  
A. X. Falcao ◽  
J. P. Papa ◽  
S. Hoshino-Shimizu

2017 ◽  
Vol 20 ◽  
pp. 61-69 ◽  
Author(s):  
Mazin Abed Mohammed ◽  
Mohd Khanapi Abd Ghani ◽  
Raed Ibraheem Hamed ◽  
Mohamad Khir Abdullah ◽  
Dheyaa Ahmed Ibrahim

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