In vitro and clinical evaluation of Umbilical Cord derived Mesenchymal Stromal Cell Conditioned Media for Hair Regeneration

2021 ◽  
Author(s):  
Caroline Mathen ◽  
Wilfrid Dsouza
Keyword(s):  
Umbilical Cord ◽  
Clinical Evaluation ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Conditioned Media ◽  
Hair Regeneration

Improvement of oocyte in vitro maturation from mice with polycystic ovary syndrome by human mesenchymal stromal cell–conditioned media

2018 ◽  
Vol 119 (12) ◽  
pp. 10365-10375 ◽  
Author(s):  
Hamideh Jafarzadeh ◽  
Hamid Nazarian ◽  
Marefat Ghaffari Novin ◽  
Zahra Shams Mofarahe ◽  
Fatemeh Eini ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Mesenchymal Stromal Cell ◽  
In Vitro Maturation ◽  
Stromal Cell ◽  
Polycystic Ovary ◽  
Conditioned Media ◽  
Ovary Syndrome

scholarly journals Extracellular Vesicles from Interferon-γ–primed Human Umbilical Cord Mesenchymal Stromal Cells Reduce Escherichia coli–induced Acute Lung Injury in Rats

2019 ◽  
Vol 130 (5) ◽  
pp. 778-790 ◽  
Author(s):  
Amir K. Varkouhi ◽  
Mirjana Jerkic ◽  
Lindsay Ormesher ◽  
Stéphane Gagnon ◽  
Sakshi Goyal ◽  
...  
Keyword(s):  
Umbilical Cord ◽  
Extracellular Vesicles ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Interferon Γ ◽  
Human Umbilical Cord ◽  
E Coli

Abstract Editor’s Perspective What We Already Know about This Topic What This Article Tells Us That Is New Background Human umbilical cord mesenchymal stromal cells possess considerable therapeutic promise for acute respiratory distress syndrome. Umbilical cord mesenchymal stromal cells may exert therapeutic effects via extracellular vesicles, while priming umbilical cord mesenchymal stromal cells may further enhance their effect. The authors investigated whether interferon-γ–primed umbilical cord mesenchymal stromal cells would generate mesenchymal stromal cell–derived extracellular vesicles with enhanced effects in Escherichia coli (E. coli) pneumonia. Methods In a university laboratory, anesthetized adult male Sprague–Dawley rats (n = 8 to 18 per group) underwent intrapulmonary E. coli instillation (5 × 109 colony forming units per kilogram), and were randomized to receive (a) primed mesenchymal stromal cell–derived extracellular vesicles, (b) naïve mesenchymal stromal cell–derived extracellular vesicles (both 100 million mesenchymal stromal cell–derived extracellular vesicles per kilogram), or (c) vehicle. Injury severity and bacterial load were assessed at 48 h. In vitro studies assessed the potential for primed and naïve mesenchymal stromal cell–derived extracellular vesicles to enhance macrophage bacterial phagocytosis and killing. Results Survival increased with primed (10 of 11 [91%]) and naïve (8 of 8 [100%]) mesenchymal stromal cell–derived extracellular vesicles compared with vehicle (12 of 18 [66.7%], P = 0.038). Primed—but not naïve—mesenchymal stromal cell–derived extracellular vesicles reduced alveolar–arterial oxygen gradient (422 ± 104, 536 ± 58, 523 ± 68 mm Hg, respectively; P = 0.008), reduced alveolar protein leak (0.7 ± 0.3, 1.4 ± 0.4, 1.5 ± 0.7 mg/ml, respectively; P = 0.003), increased lung mononuclear phagocytes (23.2 ± 6.3, 21.7 ± 5, 16.7 ± 5 respectively; P = 0.025), and reduced alveolar tumor necrosis factor alpha concentrations (29 ± 14.5, 35 ± 12.3, 47.2 ± 6.3 pg/ml, respectively; P = 0.026) compared with vehicle. Primed—but not naïve—mesenchymal stromal cell–derived extracellular vesicles enhanced endothelial nitric oxide synthase production in the injured lung (endothelial nitric oxide synthase/β-actin = 0.77 ± 0.34, 0.25 ± 0.29, 0.21 ± 0.33, respectively; P = 0.005). Both primed and naïve mesenchymal stromal cell–derived extracellular vesicles enhanced E. coli phagocytosis and bacterial killing in human acute monocytic leukemia cell line (THP-1) in vitro (36.9 ± 4, 13.3 ± 8, 0.1 ± 0.01%, respectively; P = 0.0004) compared with vehicle. Conclusions Extracellular vesicles from interferon-γ–primed human umbilical cord mesenchymal stromal cells more effectively attenuated E. coli–induced lung injury compared with extracellular vesicles from naïve mesenchymal stromal cells, potentially via enhanced macrophage phagocytosis and killing of E. coli.

Umbilical cord–derived mesenchymal stromal cell–conditioned medium exerts in vitro antiaging effects in human fibroblasts

Cytotherapy ◽  
2017 ◽  
Vol 19 (3) ◽  
pp. 371-383 ◽  
Author(s):  
Meirong Li ◽  
Yali Zhao ◽  
Haojie Hao ◽  
Liang Dong ◽  
Jiejie Liu ◽  
...  
Keyword(s):  
Conditioned Medium ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Human Fibroblasts ◽  
Cell Conditioned Medium

In vitro osteogenic potential of collagen/chitosan-based hydrogels-silica particles hybrids in human bone marrow-derived mesenchymal stromal cell cultures

2018 ◽  
Vol 113 ◽  
pp. 692-700 ◽  
Author(s):  
Joanna Filipowska ◽  
Joanna Lewandowska-Łańcucka ◽  
Adriana Gilarska ◽  
Łukasz Niedźwiedzki ◽  
Maria Nowakowska
Keyword(s):  
Bone Marrow ◽  
Cell Cultures ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Silica Particles ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteogenic Potential

scholarly journals Intravenous Human Umbilical Cord-Derived Mesenchymal Stromal Cell Administration in Models of Moderate and Severe Intracerebral Hemorrhage

2020 ◽  
Vol 29 (9) ◽  
pp. 586-598 ◽  
Author(s):  
Tanira Giara Mello ◽  
Paulo Henrique Rosado-de-Castro ◽  
Raquel Maria Pereira Campos ◽  
Juliana Ferreira Vasques ◽  
William Simões Rangel-Junior ◽  
...  
Keyword(s):  
Intracerebral Hemorrhage ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Human Umbilical Cord

Antenatal Mesenchymal Stromal Cell Extracellular Vesicle Treatment Preserves Lung Development in a Model of Bronchopulmonary Dysplasia Due to Chorioamnionitis

2021 ◽  
Author(s):  
Alison Nicole Abele ◽  
Elizabeth S Taglauer ◽  
Maricar Almeda ◽  
Noah Wilson ◽  
Abigail Abikoye ◽  
...  
Keyword(s):  
Bronchopulmonary Dysplasia ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Lung Mechanics ◽  
Spiral Artery ◽  
Lung Growth ◽  
Pulmonary Vessel ◽  
Cesarean Section Delivery ◽  
Distal Lung

Background: Antenatal stressors such as chorioamnionitis (CA) increase the risk for bronchopulmonary dysplasia (BPD). Studies have shown that experimental BPD can be ameliorated by postnatal treatment with mesenchymal stromal cell-derived extracellular vesicles (MEx). However, the antenatal efficacy of MEx to prevent BPD is unknown. Objective: To determine whether antenatal MEx therapy attenuates intrauterine inflammation and preserves lung growth in a rat model of CA-induced BPD. Methods: At embryonic day (E)20, rat litters were treated with intra-amniotic injections of saline, endotoxin (ETX) to model chorioamnionitis, MEx, or ETX plus MEx followed by cesarean section delivery with placental harvest at E22. Placental and lung evaluations were conducted at day 0 and day 14, respectively. To assess the effects of ETX and MEx on lung growth in vitro, E15 lung explants were imaged for distal branching. Results: Placental tissues from ETX-exposed pregnancies showed increased expression of inflammatory markers NLRP-3 and IL-1ß and altered spiral artery morphology. Additionally, infant rats exposed to intrauterine ETX had reduced alveolarization and pulmonary vessel density (PVD), increased right ventricular hypertrophy (RVH), and decreased lung mechanics. Intrauterine MEx therapy of ETX-exposed pups reduced inflammatory cytokines, normalized spiral artery architecture, and preserved distal lung growth and mechanics. In vitro studies showed that MEx treatment enhanced distal lung branching and increased VEGF and SPC gene expression. Conclusions: Antenatal MEx treatment preserved distal lung growth and reduced intrauterine inflammation in a model of CA-induced BPD. We speculate that MEx may provide a novel therapeutic strategy to prevent BPD due to antenatal inflammation.

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