Comparison of the paracrine activity of mesenchymal stem cells derived from human umbilical cord, amniotic membrane and adipose tissue

2017 ◽  
Vol 43 (11) ◽  
pp. 1758-1768 ◽  
Author(s):  
Filip A. Dabrowski ◽  
Anna Burdzinska ◽  
Agnieszka Kulesza ◽  
Anna Sladowska ◽  
Aleksandra Zolocinska ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Amniotic Membrane ◽  
Human Umbilical Cord

scholarly journals Osteogenic differentiation of human mesenchymal stem cells from adipose tissue and Wharton’s jelly of the umbilical cord

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
Alicja Zajdel ◽  
Magdalena Kałucka ◽  
Edyta Kokoszka-Mikołaj ◽  
Adam Wilczok
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Umbilical Cord ◽  
Wharton’S Jelly ◽  
Calcium Deposition ◽  
Human Umbilical Cord ◽  
Wharton's Jelly ◽  
Alp Activity

Induced osteogenesis of mesenchymal stem cells (MSCs) may provide an important tool for bone injures treatment. Human umbilical cord and adipose tissue are routinely discarded as clinical waste and may be used as uncontroversial MSCs sources. It still remains to be verified which source of MSCs is the most suitable for bone regeneration.The aim of this research was to investigate the osteogenic potential of human MSCs derived from adipose tissue (ASCs) and Wharton’s jelly of the human umbilical cord (WJ-MSCs) differentiated under the same conditions.Osteogenic differentiation of MSCs was detected and quantified by ARS staining for calcium deposition and alkaline phosphatase (ALP) activity, osteoprotegerin (OPG), and osteocalcin (OC) secretion measurements. Under osteogenic conditions the measured ALP activity and calcium deposition were significantly higher in ASCs than in WJ-MSCs, while the OPG and OC secretion were higher in WJ-MSCs vs. ASCs. Low concentrations of OPG and high levels of OC in ASCs and WJ-MSCs, prove that these cells reached an advanced stage of the osteogenic differentiation. The levels of OC secreted by ASCs were lower than by WJ-MSCs what indicates that the differentiation process of the ASCs reached the stage when the extracellular matrix is overproduced and the down-regulation of OC begins.Both cell types, ASCs and WJ-MSCs possess potential to differentiate towards the osteogenic lineage. However, the observed differences in the levels of osteogenic markers suggest that ASCs may be better candidates for cell-based osteogenesis than WJ-MSCs.

scholarly journals Unveiling the Differences of Secretome of Human Bone Marrow Mesenchymal Stem Cells, Adipose Tissue-Derived Stem Cells, and Human Umbilical Cord Perivascular Cells: A Proteomic Analysis

2016 ◽  
Vol 25 (14) ◽  
pp. 1073-1083 ◽  
Author(s):  
Ana O. Pires ◽  
Barbara Mendes-Pinheiro ◽  
Fábio G. Teixeira ◽  
Sandra I. Anjo ◽  
Silvina Ribeiro-Samy ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Proteomic Analysis ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Umbilical Cord ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Side-by-Side Comparison of the Biological Characteristics of Human Umbilical Cord and Adipose Tissue-Derived Mesenchymal Stem Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Li Hu ◽  
Jingqiong Hu ◽  
Jiajia Zhao ◽  
Jiarong Liu ◽  
Weixiang Ouyang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Human Adipose Tissue ◽  
Cell Types ◽  
Human Umbilical Cord ◽  
Cell Based Therapy ◽  
Secretion Profile ◽  
Immunological Phenotype

Both human adipose tissue-derived mesenchymal stem cells (ASCs) and umbilical cord-derived mesenchymal stem cells (UC-MSCs) have been explored as attractive mesenchymal stem cells (MSCs) sources, but very few parallel comparative studies of these two cell types have been made. We designed a side-by-side comparative study by isolating MSCs from the adipose tissue and umbilical cords from mothers delivering full-term babies and thus compared the various biological aspects of ASCs and UC-MSCs derived from the same individual, in one study. Both types of cells expressed cell surface markers characteristic of MSCs. ASCs and UC-MSCs both could be efficiently induced into adipocytes, osteoblasts, and neuronal phenotypes. While there were no significant differences in their osteogenic differentiation, the adipogenesis of ASCs was more prominent and efficient than UC-MSCs. In the meanwhile, ASCs responded better to neuronal induction methods, exhibiting the higher differentiation rate in a relatively shorter time. In addition, UC-MSCs exhibited a more prominent secretion profile of cytokines than ASCs. These results indicate that although ASCs and UC-MSCs share considerable similarities in their immunological phenotype and pluripotentiality, certain biological differences do exist, which might have different implications for future cell-based therapy.

scholarly journals The subpopulation of CD105 negative mesenchymal stem cells show strong immunomodulation capacity compared to CD105 positive mesenchymal stem cells

2019 ◽  
Vol 6 (4) ◽  
pp. 3131-3140 ◽  
Author(s):  
Liem Hieu Pham ◽  
Ngoc Bich Vu ◽  
Phuc Van Pham
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Immune Modulation ◽  
Human Mesenchymal Stem Cells ◽  
Human Adipose Tissue ◽  
Human Umbilical Cord ◽  
Differentiation Potential

Introduction: Human mesenchymal stem cells (MSCs) are the most popular stem cells applied in disease treatment. MSCs can be isolated and in vitro expanded from various sources such as bone marrow, peripheral blood, umbilical cord blood, umbilical cord tissue, and adipose tissue. According to Dominici et al. (2006), MSCs should express CD105, an essential marker used to confirm MSCs. However, some recent studies have show that MSCs contained a subpopulation that is negative for CD105. This study aimed to compare the immune modulation capacity of 2 populations of CD105 positive (CD105+) and negative (CD105-) MSCs derived from 2 sources: human adipose tissue (AT) and human umbilical cord (UC). Methods: MSCs were isolated from human adipose tissues (adipose tissue-derived mesenchymal stem cells – AT-MSCs) and human umbilical cord (umbilical cord-derived mesenchymal stem cells – UC-MSCs) according to previously published protocols. The two populations of CD105- and CD105+ MSCs were sorted based on the expression of CD105 from AT-MSCs and UC-MSCs. Four populations of CD105 (AT-MSCs, CD105+ AT-MSCs, CD105- UC-MSCs, and CD105+ UC-MSCs) were used to compare the phenotype as well as in vitro differentiation potential; then they were used to evaluate the immune modulation capacity by allogeneic T cell suppression and cytokine release. Results: The results showed that CD105- MSCs from AT and UC exhibited an immune modulation capacity that was much stronger than CD105+ MSCs from the same source of AT and UC. The strong immunomodulation of CD105- MSCs may relate to autocrine production of TGF-beta 1 by MSCs. Conclusion: The results suggested that CD105- MSCs are promising MSCs for application in regenerative medicine, especially for the treatment of diseases related to inflammation.  

scholarly journals Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach

Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 879
Author(s):  
Paulo Ricardo Baggio Simeoni ◽  
Rossana Baggio Simeoni ◽  
Paulo André Bispo Machado Júnior ◽  
Meila Bastos de Almeida ◽  
Dilcele Silva Moreira Dziedzic ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Tissue Regeneration ◽  
Amniotic Membrane ◽  
Cultured Cells ◽  
Human Umbilical Cord ◽  
Clinical Approach ◽  
Cartilaginous Tissue ◽  
Tracheal Injury

Acellular amniotic membrane (AM) has been studied, with promising results on the reconstruction of lesioned tissues, and has become an attractive approach for tracheal repair. This study aimed to evaluate the repair of the trachea with human umbilical cord mesenchymal stem cells (hucMSCs) differentiated in chondrocytes, grown on an experimental model. Tracheal defects were induced by surgical tracheostomy in 30 New Zealand rabbits, and the acellular amniotic membrane, with or without cells, was covering the defect. The hucMSCs were isolated and cultivated with chondrogenic differentiation over the culture of 14 days, and then grown on the AM. In this study, the AM was biocompatible and hucMSCs differentiated into chondrocytes. Our results demonstrated an important role for AM with cultured cells in the promotion of immature collagen, known to produce tissue regeneration. In addition, cartilaginous tissue was found at the tracheal defects, demonstrated by immunohistology results. This study suggests that this biomaterial implantation can be an effective future therapeutic alternative for patients with tracheal injury.

Mesenchymal stem cells derived from human umbilical cord and adipose tissue retain their properties after 24 months of cryopreservation

Cytotherapy ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. S37-S38
Author(s):  
Chee-Yin Wong ◽  
Ghee-Chien Ooi ◽  
Wai-Ping Vivian Kong ◽  
Ming-Foong Chai ◽  
Kalaivani Thiagarajah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Human Umbilical Cord

scholarly journals Human Umbilical Cord Mesenchymal Stem Cell Promotes Angiogenesis via Integrin β1/ERK1/2/HIF-1α/VEGF-A Signaling Pathway for off-the-shelf Breast Tissue Engineering

2021 ◽  
Author(s):  
Mian Wu ◽  
Lifeng Chen ◽  
Yuhan Qi ◽  
Hai Ci ◽  
Shan Mou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Breast Tissue ◽  
Control Group ◽  
Human Umbilical Cord ◽  
Fat Tissue ◽  
Integrin Β1

Abstract Background: Mesenchymal stem cells (MSC) based tissue engineered breast represent the visible future for breast reconstruction after mastectomy. However, autologous MSCs might not be appropriate for the large graft construction due to cell senescence during excessive cell expansion, thus hindering its further off-the-shelf application. As human umbilical cord stem cells (hUCMSCs) have been proved to be safe with low immune response and easily stored, they are ideal for off-the-shelf tissue engineering application. Here, we aim to explore the possibility of umbilical cord mesenchymal stem cells as tissue-engineered breast seed cells. Methods: The allogenic hUCMSCs were injected into transplanted fat tissue with or without breast scaffolds as an alternative for breast tissue engineering in vivo, and its potential mechanism of angiogenesis in vitro was explored.Results: The hUCMSCs promoted proliferation, migration, and angiogenesis of human umbilical vein endothelial cells (HUVECs) through paracrine process by activating the integrin β1/ERK1/2/HIF-1α/VEGF-A signaling pathway. Histological examination of grafted fat revealed that the group which received hUCMSCs transplantation had more fat tissue ((93.60±2.40) %) and less MAC2+CD206- M1 macrophages ((0.50±0.47) cells/field) compared to control group (fat tissue (45.42±5.96) and macrophage cells/field (5.00±2.23)). Moreover, the cell tracing dye labeled hUCMSCs were confirmed to differentiate into adipocytes and vascular endothelial cells in adipose tissue. Additionally, when applied to the tissue-engineered breast with scaffold, the group treat with hUCMSCs had more adipose tissues and CD31+ cells than the control group.Conclusions: Our findings demonstrate the role of allogeneic hUCMSCs in regenerating adipose tissue and may provide a new strategy to construct tissue engineered breast.

scholarly journals Human umbilical cord mesenchymal stem cells express cholinergic neuron markers during co-culture with amniotic membrane cells and retinoic acid induction

2021 ◽  
Author(s):  
Mohammad-Reza Kouchakian ◽  
Morteza Koruji ◽  
Mohammad Najafi ◽  
Seyedeh Farzaneh Moniri ◽  
Alireza Asgari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Retinoic Acid ◽  
Umbilical Cord ◽  
Amniotic Membrane ◽  
Cholinergic Neuron ◽  
Human Umbilical Cord
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