Responses of some innate immune‐genes involved in the toll‐pathway in black tiger shrimp ( Penaeus monodon ) to Vibrio harveyi infection and on exposure to ligands in vitro

2020 ◽  
Vol 51 (6) ◽  
pp. 1419-1429 ◽  
Author(s):  
Anand Deepika ◽  
Krishnan Sreedharan ◽  
Kooloth Valappil Rajendran
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Innate Immune ◽  
Toll Pathway ◽  
Immune Genes ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp ◽  
Innate Immune Genes

scholarly journals Response of hemocytes profile in the black tiger shrimp (Penaeus monodon) against Vibrio harveyi induced by Xylocarpus granatum leaves extract

2020 ◽  
Vol 13 (4) ◽  
pp. 751-757 ◽  
Author(s):  
Gina Saptiani ◽  
A. Syafei Sidik ◽  
Fikri Ardhani ◽  
Esti Handayani Hardi
Keyword(s):  
Survival Rate ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Water Extract ◽  
Negative Control ◽  
Distilled Water ◽  
Black Tiger Shrimp ◽  
Xylocarpus Granatum ◽  
Tiger Shrimp ◽  
Immersing Method

Aim: The present study investigated hemocytes profile of black tiger shrimp (Penaeus monodon) induced with Xylocarpus granatum leaves extract to protect against Vibrio harveyi infection. Materials and Methods: X. granatum leaves were chopped into small size, air-dried, and extracted with one of the following solvents: Ethanol, distilled water, and seawater, whereas each solvent was given in three different concentrations (750 ppm, 1.000 ppm, and 1.250 ppm, respectively). Extracts were induced to 60 post-larvae shrimp in each treatment (three replicates, 20 shrimp for each) by immersing method and subsequently challenged with V. harveyi. Results: This study demonstrated different effectiveness among solvents used to extract X. granatum leaves, in which distilled water showed the most effective solvent as can be seen from the lowest percentage on anorexia, lethargic, and weakened reflex of shrimp compared with another solvent, positive and negative controls. Pathological symptoms for shrimp induced by X. granatum leaves extract were minimum with the highest survival rate compared with those of positive and negative control. Total hemocyte cells and its cell constituents such as semi-granular, granular, and hyaline cells on treatment group at 1.250 ppm were higher than controls. Conclusion: Leaves extract of X. granatum extract effectively inhibited V. harveyi infection, increased survival rate, and hemocytes cell of the experimental shrimp. Distilled water extract of X. granatum at 1.250 ppm demonstrated the highest protective effect toward V. harveyi infection on P. monodon.

scholarly journals THE EFFECT OF MARINE SPONGE Aaptos aaptos EXTRACT IN VIBRIOSIS TREATMENT OF BLACK TIGER SHRIMP Penaeus monodon LARVAE

2014 ◽  
Vol 9 (2) ◽  
pp. 103
Author(s):  
Rosmiati Rosmiati ◽  
Najiah Musa ◽  
Habsah Mohammad ◽  
Tengku Sifzizul Tengku Muhammad ◽  
Andi Parenrengi ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Marine Sponge ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Toxicity Study ◽  
Histological Observation ◽  
Black Tiger Shrimp ◽  
Butanol Extract ◽  
Tiger Shrimp ◽  
Bioactive Potential

Black tiger shrimp Penaeus monodon post larvae were challenged with Vibrio harveyi and butanol extract of selected marine sponge Aaptos aaptos to determine its antibacterial bioactive potential in vibriosis treatment. Based on the preliminary toxicity study, the A. aaptos butanol extract with concentrations of 31.25, 62.5, and 125 mg/L were selected in the study. Black tiger shrimp post larvae were challenged with V. harveyi at 107 cfu/mL and immersed A. aaptos butanol extract with the concentration of 125 mg/L showed significantly in (P<0.05) decrease mortality of the post larvae treated. Besides at this concentration, V. harveyi population in the rearing water and the post larvae treated decreased compared to control (untreated post larvae). Histological observation indicated that there was no changing on hepatopancreas of the black tiger shrimp post larvae. Based on this result, it is suggested that the butanol extract of A. aaptos is a potential bioactive compounds source in the treatment of vibriosis which may replaced the current antibiotics application.

scholarly journals Screening of probiotics bacteria from coral reef using co-culture method for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae

2015 ◽  
Vol 10 (2) ◽  
pp. 174
Author(s):  
Ade Dwi Sasanti ◽  
Widanarni . ◽  
Sukenda .
Keyword(s):  
Coral Reef ◽  
Survival Rate ◽  
16S Rrna ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Culture Method ◽  
In Vivo Test ◽  
Tiger Shrimp

<p>ABSTRACT</p><p><br />This study was carried out to obtain bacteria isolates from coral reef using co-culture method which potentially inhibit Vibrio harveyi growth. A total of 110 isolates were isolated from Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., and Haliophora sp., and were screened for their antagonistic activity against V. harveyi in in vitro and in vivo test. Five candidate probiotics (5H1 candidate probiotics isolated from Acropora sp., 11I and 11G isolates isolated from Hystrix sp. and 13B and 13G1 isolates isolated from Poecillophora sp., was able to inhibit growth of V. harveyi MR5339 RFR up to 101‒102 cfu/mL. Two isolates (13B and 13G1) were not pathogenic at concentration 106 cfu/mL bacteria and could increase of survival rate of tiger shrimp (Penaeus monodon) larvae in in vivo test. Survival rate of tiger shrimp larvae that treatment with 13B and 13G1 were 86,67% and 88,33%, and have a significant different with positive control (61,67%). Partial sequencing of 16S-rRNA showed that 13G1 isolate was similar to V. alginolyticus.<br />Keywords: vibriosis, Vibrio harveyi, tiger shrimp, probiotic, coral reef</p><p><br />ABSTRAK</p><p><br />Penelitian ini bertujuan untuk mendapatkan bakteri probiotik asal terumbu karang dengan metode kultur bersama untuk pengendalian penyakit vibriosis pada larva udang windu (Penaeus monodon). Sebanyak 110 isolat berhasil diisolasi dari Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., dan Heliophora sp. dan dilakukan penapisan untuk melihat aktivitas kemampuannya melawan Vibrio harveyi MR 5339 RfR dalam uji in vitro dan in vivo. Sebanyak 56 isolat menghasilkan daya hambat terhadap V. harveyi MR5339 RfR pada metode kultur bersama. Lima isolat kandidat probiotik (isolate 5H1 diisolasi dari Acropora sp., isolat 11I dan 11G diisolasi dari Hystrix sp., serta isolat 13B dan 13G1 yang diisolasi dari Poecillophora sp.), mampu menekan pertumbuhan V. harveyi MR5339 RfR hingga 101–102 cfu/mL. Kedua isolat (13B dan 13G1) terbukti tidak bersifat patogen pada konsentrasi 106 cfu/mL dan mampu meningkatkan sintasan larva udang windu pada uji in vivo. Nilai sintasan larva pada perlakuan yang diberi kandidat probiotik 13B dan 13G1 berturut-turut adalah 86,67% dan 88,33%, namun berbeda nyata dengan kontrol positif (61,67%). Hasil analisis sekuen sebagian gen 16S-rRNA menunjukkan bahwa isolat 13G1 termasuk spesies V. algynoliticus.<br /><br />Kata kunci: vibriosis, Vibrio harveyi, udang windu, probiotik, terumbu karang</p>

scholarly journals Expression of Toll-like receptors (TLR), in lymphoid organ of black tiger shrimp (Penaeus monodon) in response to Vibrio harveyi infection

2015 ◽  
Vol 1 ◽  
pp. 1-4 ◽  
Author(s):  
Mundanda Muthappa Dechamma ◽  
Moger Rajeish ◽  
Biswajit Maiti ◽  
Madhu K. Mani ◽  
Indrani Karunasagar
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Lymphoid Organ ◽  
Toll Like Receptors ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp

scholarly journals Immune-related genes in hemocytes of the black tiger shrimp, Penaeus monodon, infected with Vibrio harveyi

2002 ◽  
Vol 68 (sup2) ◽  
pp. 1083-1086 ◽  
Author(s):  
PREMRUETHAI SUPUNGUL ◽  
SIRAWUT KLINBUNGA ◽  
RATH PICHYANGKURA ◽  
IKUO HIRONO ◽  
TAKASHI AOKI ◽  
...  
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp ◽  
Immune Related Genes

scholarly journals Inhibitory Mechanism of Robiotic Bacteria on The Growth of Vibrio harveyi in Tiger Shrimp (Penaeus monodon) Larvae

2008 ◽  
Vol 7 (2) ◽  
pp. 179
Author(s):  
. Widanarni ◽  
E. Ayuzar ◽  
. Sukenda
Keyword(s):  
Survival Rate ◽  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Vibrio Alginolyticus ◽  
Probiotic Bacteria ◽  
Rrna Gene ◽  
Inhibitory Mechanism ◽  
Water Culture ◽  
Tiger Shrimp

<p>Three probiotics named SKT-b, 1Ub, and Ua had inhibitory activity against the growth of <em>Vibrio harveyi</em>. These strains were mutated by rifampicin resistant. The inhibitory effect of SKT-b,1Ub, and Ua on the growth of <em>V. harveyi</em> was investigated by concomitant incubation of the two bacteria in a culture shrimp larvae. Colony forming unit of <em>V. harveyi</em>, probiotic and total of bacteria in dead, live larvae and water culture was monitored, and survival rate of larvae was investigated. Shrimp inoculated probiotic previously had survival rate higher than control (without probiotic). Number of <em>V. harveyi </em>in treatment without probiotic inoculation also higher compared to treatment with probiotic inoculation in dead, live larvae and water culture.  It demonstrated possible inhibition of probiotic bacteria on <em>V. harveyi</em> through competition for adherence sites or nutrition source. Partial sequencing of 16S-rRNA gene showed that 1Ub was similar to <em>Pseudoalteromonas piscicida</em>, whereas SKT-b and Ua were similar to <em>Vibrio alginolyticus</em>.</p> <p>Keywords: probiotic bacteria, inhibitory mechanism, <em>V. harveyi</em>, tiger shrimp</p> <p> </p> <p>ABSTRAK</p> <p>Tiga isolat bakteri probiotik yaitu 1Ub, SKT-b dan Ua telah diuji memiliki aktivitas penghambatan terhadap pertumbuhan<em> V. harveyi </em>secara <em>in vitro</em>. Ketiga isolat ini kemudian diberi penanda resisten rifampisin (Rf<sup>R</sup>) melalui mutasi spontan untuk mempelajari mekanisme penghambatannya pada larva udang windu.  Efek penghambatan dari 1Ub, SKT-b dan Ua terhadap pertumbuhan <em>V. harveyi</em> diamati melalui pemberian secara bersamaan antara bakteri probiotik dan <em>V. harveyi</em> tersebut dalam air pemeliharaan larva udang.  Jumlah sel bakteri probiotik,<em> V. harveyi</em> dan total bakteri baik pada larva mati, larva hidup dan air pemeliharaan diamati dan kelangsungan hidup larva dihitung.  Nilai kelangsungan hidup udang pada perlakuan yang diinokulasi bakteri probiotik lebih tinggi daripada kontrol (tanpa penambahan bakteri probiotik). Jumlah sel <em>V. harveyi</em> pada perlakuan tanpa penambahan probiotik juga lebih tinggi, dibanding pada perlakuan dengan penambahan probiotik baik pada larva mati, larva hidup maupun air media pemeliharaan. Hal ini menunjukkan adanya penghambatan bakteri probiotik terhadap <em>V. harveyi</em> yang kemungkinan melalui kompetisi tempat pelekatan atau sumber nutrisi. Hasil analisis sekuen sebagian gen 16-rRNA menunjukkan bahwa isolat 1Ub termasuk spesies <em>Pseudoalteromonas piscicida, </em>sedangkan SKT-b dan Ua termasuk spesies <em>Vibrio alginolyticus</em>. </p> <p>Kata kunci: bakteri probiotik, mekanisme penghambatan, <em>V. harveyi</em>, udang windu</p>

Solvent extracts of the red seaweed Gracilaria fisheri prevent Vibrio harveyi infections in the black tiger shrimp Penaeus monodon

2011 ◽  
Vol 30 (1) ◽  
pp. 389-396 ◽  
Author(s):  
Kulwadee Kanjana ◽  
Tawut Radtanatip ◽  
Somluk Asuvapongpatana ◽  
Boonsirm Withyachumnarnkul ◽  
Kanokpan Wongprasert
Keyword(s):  
Vibrio Harveyi ◽  
Penaeus Monodon ◽  
Red Seaweed ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp
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