Chromium Binding Bacillus cereus VITSH1‐ A Promising Candidate for Heavy Metal Clean up

Bacillus cereus NWUAB01 was isolated from a gold-mining site in Vryburg, South Africa, for its multiple heavy metal resistance properties. Here, we report the draft genome sequence of B. cereus NWUAB01 obtained with Illumina sequencing.

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Genomic analysis of Bacillus cereus NWUAB01 and its heavy metal removal from polluted soil

Scientific Reports ◽

10.1038/s41598-020-75170-x ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Ayansina Segun Ayangbenro ◽

Olubukola Oluranti Babalola

Keyword(s):

Heavy Metal ◽

Surface Tension ◽

Bacillus Cereus ◽

Metal Removal ◽

Heavy Metal Removal ◽

Gene Clusters ◽

Industrial Applications ◽

Luria Bertani ◽

Biosurfactant Production ◽

Screening Methods

AbstractMicroorganisms that display unique biotechnological characteristics are usually selected for industrial applications. Bacillus cereus NWUAB01 was isolated from a mining soil and its heavy metal resistance was determined on Luria–Bertani agar. The biosurfactant production was determined by screening methods such as drop collapse, emulsification and surface tension measurement. The biosurfactant produced was evaluated for metal removal (100 mg/L of each metal) from contaminated soil. The genome of the organism was sequenced using Illumina Miseq platform. Strain NWUAB01 tolerated 200 mg/L of Cd and Cr, and was also tolerant to 1000 mg/L of Pb. The biosurfactant was characterised as a lipopeptide with a metal-complexing property. The biosurfactant had a surface tension of 39.5 mN/m with metal removal efficiency of 69%, 54% and 43% for Pb, Cd and Cr respectively. The genome revealed genes responsible for metal transport/resistance and biosynthetic gene clusters involved in the synthesis of various secondary metabolites. Putative genes for transport/resistance to cadmium, chromium, copper, arsenic, lead and zinc were present in the genome. Genes responsible for biopolymer synthesis were also present in the genome. This study highlights biosurfactant production and heavy metal removal of strain NWUAB01 that can be harnessed for biotechnological applications.

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An antibiotic, heavy metal resistant and halotolerant Bacillus cereus SIU1 and its thermoalkaline protease

Microbial Cell Factories ◽

10.1186/1475-2859-9-59 ◽

2010 ◽

Vol 9 (1) ◽

pp. 59 ◽

Cited By ~ 44

Author(s):

Sanjay K Singh ◽

Vinayak R Tripathi ◽

Rakesh K Jain ◽

Surendra Vikram ◽

Satyendra K Garg

Keyword(s):

Heavy Metal ◽

Bacillus Cereus ◽

Heavy Metal Resistant

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Alteration of Heavy Metal Fractions in Soil of Guiyu, South China by Native Bacillus cereus under the Effect of BDE209

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.726-731.3901 ◽

2013 ◽

Vol 726-731 ◽

pp. 3901-3907

Author(s):

Hui Peng ◽

Hua Yin ◽

Jin Shao Ye

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Bacillus Cereus ◽

Sequential Extraction ◽

South China ◽

Metal Speciation ◽

Interaction Time ◽

Icp Oes ◽

Decabromodiphenyl Ether ◽

Concentration Variation

Microbiologic experiments together with Tessier sequential extraction and ICP-OES determination showed that distributions of Pb, Cu, Cd and Cr in soil of Guiyu, an e-waste dismantling region in South China, have changed after activities of native combined Bacillus cereus. Exchangeable fractions of these heavy metals were 1.52, 12.32, 3.98 and 34.50-fold those of original ones after 8 h interaction, here the organic phases were supposed to be the main alteration origin. The changing interaction time brought different impacts on concentration variation of heavy metals. Decabromodiphenyl ether (BDE209) influenced combined Bacillus cereus to redistribute the shares of heavy metals. BDE209 of 1 mg/L favored the alteration to make heavy metals become more bioavailable, while 5 mg/L and 10 mg/L of BDE209 baffled this alteration. However, influences of these strains on heavy metal speciation could be generally enhanced at the presence of BDE209, particularly for Cu and Pb Therefore, non-residual fractions were easier to be transferred into bioavailable ones after interaction with biomass, thus favoring the subsequent bioremediation of soil.

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Role of Bacillus cereus in Improving the Growth and Phytoextractability of Brassica nigra (L.) K. Koch in Chromium Contaminated Soil

Molecules ◽

10.3390/molecules26061569 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1569

Author(s):

Nosheen Akhtar ◽

Noshin Ilyas ◽

Humaira Yasmin ◽

R. Z. Sayyed ◽

Zuhair Hasnain ◽

...

Keyword(s):

Heavy Metal ◽

Seed Germination ◽

Plant Growth ◽

Bacillus Cereus ◽

Contaminated Soil ◽

Metal Tolerance ◽

Heavy Metal Tolerance ◽

Bacterial Strains ◽

Bacterial Inoculation ◽

Phytoextraction Potential

Plant growth-promoting rhizobacteria (PGPR) mediate heavy metal tolerance and improve phytoextraction potential in plants. The present research was conducted to find the potential of bacterial strains in improving the growth and phytoextraction abilities of Brassica nigra (L.) K. Koch. in chromium contaminated soil. In this study, a total of 15 bacterial strains were isolated from heavy metal polluted soil and were screened for their heavy metal tolerance and plant growth promotion potential. The most efficient strain was identified by 16S rRNA gene sequencing and was identified as Bacillus cereus. The isolate also showed the potential to solubilize phosphate and synthesize siderophore, phytohormones (indole acetic acid, cytokinin, and abscisic acid), and osmolyte (proline and sugar) in chromium (Cr+3) supplemented medium. The results of the present study showed that chromium stress has negative effects on seed germination and plant growth in B. nigra while inoculation of B. cereus improved plant growth and reduced chromium toxicity. The increase in seed germination percentage, shoot length, and root length was 28.07%, 35.86%, 19.11% while the fresh and dry biomass of the plant increased by 48.00% and 62.16%, respectively, as compared to the uninoculated/control plants. The photosynthetic pigments were also improved by bacterial inoculation as compared to untreated stress-exposed plants, i.e., increase in chlorophyll a, chlorophyll b, chlorophyll a + b, and carotenoid was d 25.94%, 10.65%, 20.35%, and 44.30%, respectively. Bacterial inoculation also resulted in osmotic adjustment (proline 8.76% and sugar 28.71%) and maintained the membrane stability (51.39%) which was also indicated by reduced malondialdehyde content (59.53% decrease). The antioxidant enzyme activities were also improved to 35.90% (superoxide dismutase), 59.61% (peroxide), and 33.33% (catalase) in inoculated stress-exposed plants as compared to the control plants. B. cereus inoculation also improved the uptake, bioaccumulation, and translocation of Cr in the plant. Data showed that B. cereus also increased Cr content in the root (2.71-fold) and shoot (4.01-fold), its bioaccumulation (2.71-fold in root and 4.03-fold in the shoot) and translocation (40%) was also high in B. nigra. The data revealed that B. cereus is a multifarious PGPR that efficiently tolerates heavy metal ions (Cr+3) and it can be used to enhance the growth and phytoextraction potential of B. nigra in heavy metal contaminated soil.

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Identification Of A Unique Mechanism Of Tolerance Against Nickel In Bacillus Cereus Isolated From Heavy Metal Contaminated Sites.

The Internet Journal of Microbiology ◽

10.5580/1caf ◽

2010 ◽

Vol 9 (1) ◽

Cited By ~ 1

Keyword(s):

Heavy Metal ◽

Bacillus Cereus ◽

Contaminated Sites ◽

Unique Mechanism

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Bacterial Tolerance to Heavy Metal Contents Present in Contaminated and Uncontaminated Soils

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v29i2.28437 ◽

2016 ◽

Vol 29 (2) ◽

pp. 56-61

Author(s):

FZ Tanu ◽

S Hoque

Keyword(s):

Heavy Metal ◽

Bacillus Subtilis ◽

Bacillus Cereus ◽

Metal Tolerance ◽

Micrococcus Luteus ◽

Heavy Metal Tolerance ◽

Subtilis Strain ◽

Rrna Gene ◽

Bacterial Strains ◽

Metal Tolerant Bacteria

Present study dealt with identification of some heavy metal tolerant bacteria from contaminated industrial soils of Dhaka Export Processing Zone (DEPZ) at Savar, tannery area at Hazaribagh and uncontaminated agricultural soils of Dhamrai and Kushtia in Bangladesh and determination of their tolerance to chromium (Cr6+) and cadmium (Cd2+). A total of 15 isolates from four soil samples were provisionally identified as different species of Bacillus, Micrococcus and Pseudomonas based on their morphological, physiological, and biochemical characteristics. Among them eight colonies were separated based on high level of heavy metal tolerance and identified at molecular level by PCR technique and 16S rRNA gene sequencing as Micrococcus luteus strain P43 (E4), Bacillus pocheonensis strain TR2-6 (T6), Bacillus megaterium strain H2 (T8), Bacillus amyloliquefaciens strain SCSAAB0007 (D10), Bacillus cereus isolate PGBw4 (D11), Bacillus cereus strain ES-4a1 (K12), Bacillus subtilis strain 1320, (K13), and Bacillus subtilis strain DP14 (K14). The Maximum Tolerable Concentration (MTC) of bacterial strains to Cr6+ and Cd2+ ranged between 250-1250 ?g/ml and 30-150 ?g/ml, respectively in nutrient broth medium. From the metal tolerance investigation Bacillus was found as the most heavy metal tolerant to both Cr6+ and Cd2+ among the three genera. The identified heavy metal tolerant bacteria could be useful for the bioremediation of heavy metal contaminated environment.Bangladesh J Microbiol, Volume 29, Number 2, Dec 2012, pp 56-61

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Synthesis of Nano-Strontium Titanate Immobilised on the Silica Gel G and its Application in Removal of Heavy Metal Ions from Water

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.194-196.751 ◽

2011 ◽

Vol 194-196 ◽

pp. 751-754

Author(s):

Dong Zhang ◽

Yan Cheng

Keyword(s):

Heavy Metal ◽

Metal Ions ◽

Adsorption Capacity ◽

Silica Gel ◽

Strontium Titanate ◽

Heavy Metal Ions ◽

X Ray Diffraction ◽

Promising Candidate ◽

Experimental Conditions ◽

Mixture System

A new adsorption agent, nanosized strontium titanate immobilized on the silica gel G (GSTO) was prepared, and it was characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The ability of the new adsorption to remove heavy metal Pb, Cd, Zn, Cu, Ni and Mn from water samples was assessed. The research results displayed that adsorbent has the highest adsorption capacity for Pb, Cd, Zn, Cu, Ni and Mn in ions mixture system. Optimal experimental conditions including pH and contact time have been established. Desorption studies were carried out successfully with diluted HNO3 solutions. Thus, nano-strontium titanate immobilized on the silica gel G is favorable and useful for the removal of these heavy metal ions, and the high adsorption capacity makes it a good promising candidate material for Pb, Cd, Zn, Cu, Ni and Mn removal.

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