Identification Of A Unique Mechanism Of Tolerance Against Nickel In Bacillus Cereus Isolated From Heavy Metal Contaminated Sites.

10.5580/1caf ◽  
2010 ◽  
Vol 9 (1) ◽  
Keyword(s):  
Heavy Metal ◽  
Bacillus Cereus ◽  
Contaminated Sites ◽  
Unique Mechanism

Ecotoxicological and microbiological characterization of soils from heavy-metal- and hydrocarbon-contaminated sites

2009 ◽  
Vol 163 (1-4) ◽  
pp. 477-488 ◽  
Author(s):  
Grażyna A. Płaza ◽  
Grzegorz Nałęcz-Jawecki ◽  
Onruthai Pinyakong ◽  
Paul Illmer ◽  
Rosa Margesin
Keyword(s):  
Heavy Metal ◽  
Contaminated Sites

scholarly journals Investigations on Engineering Properties of Solidified/Stabilized Pb-Contaminated Soil Based on Alkaline Residue

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Fusheng Zha ◽  
Dongdong Pan ◽  
Long Xu ◽  
Bo Kang ◽  
Chengbin Yang ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Contaminated Soil ◽  
Microstructural Analysis ◽  
Engineering Properties ◽  
Contaminated Sites ◽  
Adsorptive Capacity ◽  
Curing Time ◽  
Test Results ◽  
Hydration Products ◽  
Microstructure Characteristics

Solidification/stabilization (S/S) has been considered as one of the most effective techniques for remediation of the heavy metal-contaminated sites. Among various binders adopted in S/S, alkaline residue (AR) could be considered as a new binder to treat heavy metal-contaminated soil due to its strong adsorptive capacity for heavy metal ions. So in this paper, the strength, leaching, and microstructure characteristics of the solidified/stabilized Pb-contaminated soil by using alkaline residue are systematically investigated. Test results present that the unconfined compressive strength (UCS) of the treated soil will increase, while the leached Pb2+ concentration will decrease, with the increase of the alkaline residue content in the specimen. The UCS increases significantly with the curing time increasing during the initial 28 days, after which the UCS of the specimen becomes stable. The leached Pb2+ concentration decreases significantly at the initial 28 days followed by a stable trend with curing time increasing. The UCS decreases and the leached Pb2+ concentration increases with the increase of the initial Pb2+ concentration in the specimen. The microstructural analysis performed by scanning electron microscope (SEM) showed that the increase of the alkaline residue content and curing time will result in more hydration products and densified microstructure, which could effectively improve the engineering properties of the specimen.

scholarly journals Evaluation of Phytoremediation Potential of Castor Cultivars for Heavy Metals from Soil

2019 ◽  
Vol 37 ◽  
Author(s):  
M.J. KHAN ◽  
N. AHMED ◽  
W. HASSAN ◽  
T. SABA ◽  
S. KHAN ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Contaminated Soils ◽  
Metal Uptake ◽  
Plant Biomass ◽  
Ricinus Communis ◽  
Contaminated Sites ◽  
Heavy Metal Uptake ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT: Phytoremediation is a useful tool to restore heavy metals contaminated soils. This study was carried out to test two castor (Ricinus communis) cultivars [Local and DS-30] for phytoextraction of heavy metals from the soil spiked by known concentrations of seven metals (Cu, Cr, Fe, Mn, Ni, Pb and Zn). A pot experiment was laid out by using a completely randomized design. Soil and plant samples were analyzed at 100 days after planting. The data on heavy metal uptake by plant tissues (roots, leaves and shoots) of the two castor cultivars suggested that a considerable amount of metals (Fe = 27.18 mg L-1; Cu = 5.06 mg L-1; Cr = 2.95 mg L-1; Mn = 0.22 mg L-1; Ni = 4.66 mg L-1; Pb = 3.33 mg L-1; Zn = 15.04 mg L-1) was accumulated in the plant biomass. The soil heavy metal content at the end of experiment significantly decreased with both cultivars, resulting in improved soil quality. Therefore, it is concluded that both castor cultivars, Local and DS-30, can be used for phytoremediation of heavy metal-contaminated sites.

scholarly journals Effect of Temperature and Cell Viability on Uranium Biomineralization by the Uranium Mine Isolate Penicillium simplicissimum

2021 ◽  
Vol 12 ◽  
Author(s):  
Sebastian Schaefer ◽  
Robin Steudtner ◽  
René Hübner ◽  
Evelyn Krawczyk-Bärsch ◽  
Mohamed L. Merroun
Keyword(s):  
Heavy Metal ◽  
Cell Viability ◽  
Laser Fluorescence ◽  
Contaminated Sites ◽  
Effect Of Temperature ◽  
Uranium Mine ◽  
Penicillium Simplicissimum ◽  
Removal Capacity ◽  
Viable Cells ◽  
Dry Biomass

The remediation of heavy-metal-contaminated sites represents a serious environmental problem worldwide. Currently, cost- and time-intensive chemical treatments are usually performed. Bioremediation by heavy-metal-tolerant microorganisms is considered a more eco-friendly and comparatively cheap alternative. The fungus Penicillium simplicissimum KS1, isolated from the flooding water of a former uranium (U) mine in Germany, shows promising U bioremediation potential mainly through biomineralization. The adaption of P. simplicissimum KS1 to heavy-metal-contaminated sites is indicated by an increased U removal capacity of up to 550 mg U per g dry biomass, compared to the non-heavy-metal-exposed P. simplicissimum reference strain DSM 62867 (200 mg U per g dry biomass). In addition, the effect of temperature and cell viability of P. simplicissimum KS1 on U biomineralization was investigated. While viable cells at 30°C removed U mainly extracellularly via metabolism-dependent biomineralization, a decrease in temperature to 4°C or use of dead-autoclaved cells at 30°C revealed increased occurrence of passive biosorption and bioaccumulation, as confirmed by scanning transmission electron microscopy. The precipitated U species were assigned to uranyl phosphates with a structure similar to that of autunite, via cryo-time-resolved laser fluorescence spectroscopy. The major involvement of phosphates in U precipitation by P. simplicissimum KS1 was additionally supported by the observation of increased phosphatase activity for viable cells at 30°C. Furthermore, viable cells actively secreted small molecules, most likely phosphorylated amino acids, which interacted with U in the supernatant and were not detected in experiments with dead-autoclaved cells. Our study provides new insights into the influence of temperature and cell viability on U phosphate biomineralization by fungi, and furthermore highlight the potential use of P. simplicissimum KS1 particularly for U bioremediation purposes.Graphical Abstract

scholarly journals A Review on the Resistance and Accumulation of Heavy Metals by Different Microbial Strains

2022 ◽  
Author(s):  
Madhuri Girdhar ◽  
Zeba Tabassum ◽  
Kopal Singh ◽  
Anand Mohan
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Volcanic Eruptions ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Contaminated Sites ◽  
Bacillus Species ◽  
Polluted Sites ◽  
Pollution Accumulation ◽  
Microbial Strains

Heavy metals accumulated the earth crust and causes extreme pollution. Accumulation of rich concentrations of heavy metals in environments can cause various human diseases which risks health and high ecological issues. Mercury, arsenic, lead, silver, cadmium, chromium, etc. are some heavy metals harmful to organisms at even very low concentration. Heavy metal pollution is increasing day by day due to industrialization, urbanization, mining, volcanic eruptions, weathering of rocks, etc. Different microbial strains have developed very efficient and unique mechanisms for tolerating heavy metals in polluted sites with eco-friendly techniques. Heavy metals are group of metals with density more than 5 g/cm3. Microorganisms are generally present in contaminated sites of heavy metals and they develop new strategies which are metabolism dependent or independent to tackle with the adverse effects of heavy metals. Bacteria, Algae, Fungi, Cyanobacteria uses in bioremediation technique and acts a biosorbent. Removal of heavy metal from contaminated sites using microbial strains is cheaper alternative. Mostly species involved in bioremediation include Enterobacter and Pseudomonas species and some of bacillus species too in bacteria. Aspergillus and Penicillin species used in heavy metal resistance in fungi. Various species of the brown algae and Cyanobacteria shows resistance in algae.

Bacterial Remediation of Chromium From Industrial Sludge

2021 ◽  
pp. 97-125
Author(s):  
Dipankar Roy ◽  
Arup Kumar Mitra
Keyword(s):  
Heavy Metals ◽  
Bacillus Cereus ◽  
Toxic Metals ◽  
Growth Pattern ◽  
Contaminated Sites ◽  
Total Chromium ◽  
Industrial Sludge ◽  
Gram Negative ◽  
Living Organisms ◽  
Chromium Uptake

Chromium-like heavy toxic metals seriously influence the metabolism of living organisms and cause permanent threatening of health. Microorganisms can help to detoxify those hazardous heavy metals in the environment by the process of bioremediation. Two bacterial genera were isolated from industrial sludge designated P1 and P2. From the 16srRNA study, it is revealed that P1 is Bacillus cereus and P2 is Enterobacter sp. They are deposited in NCMR and NCBI and received the accession no. MCC 3868 for P1 and MCC 3788 for P2. P1 is gram positive, motile, and P2 is gram negative, motile. Eighteen antibiotics have been taken for antibiotic assay; P1 is resistant to 12; P2 is resistant to 8 antibiotics. For growth pattern analysis in chromium, three parameters have been selected, and they are temperature, pH, and biomass. In LD50 and above parameters, total chromium uptake by those bacteria in stressed conditions have been recorded. The two bacteria are not antagonistic to each other so they are used to bioremediate chromium from their contaminated sites and also treated as consortium.

Aerobic Chromate Reducing Bacillus cereus Isolated from the Heavy Metal Contaminated Ennore Creek Sediment, North of Chennai, Tamil Nadu, South East India

2007 ◽  
Vol 2 (2) ◽  
pp. 133-140 ◽  
Author(s):  
S. Kamala Kannan . ◽  
K.J. Lee . ◽  
R. Krishnamoorthy . ◽  
A. Purusothaman . ◽  
K. Shanthi . ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Bacillus Cereus ◽  
Tamil Nadu

scholarly journals Effects of Ectomycorrhizal Fungi and Heavy Metals (Pb, Zn, and Cd) on Growth and Mineral Nutrition of Pinus halepensis Seedlings in North Africa

2020 ◽  
Vol 8 (12) ◽  
pp. 2033
Author(s):  
Chadlia Hachani ◽  
Mohammed S. Lamhamedi ◽  
Claudio Cameselle ◽  
Susana Gouveia ◽  
Abdenbi Zine El Abidine ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
North Africa ◽  
Mineral Nutrition ◽  
Contaminated Soils ◽  
Mycorrhizal Fungi ◽  
Pinus Halepensis ◽  
Translocation Factor ◽  
Contaminated Sites ◽  
High Concentrations

The pollution of soils by heavy metals resulting from mining activities is one of the major environmental problems in North Africa. Mycorrhizoremediation using mycorrhizal fungi and adapted plant species is emerging as one of the most innovative methods to remediate heavy metal pollution. This study aims to assess the growth and the nutritional status of ectomycorrhizal Pinus halepensis seedlings subjected to high concentrations of Pb, Zn, and Cd for possible integration in the restoration of heavy metals contaminated sites. Ectomycorrhizal and non-ectomycorrhizal P. halepensis seedlings were grown in uncontaminated (control) and contaminated soils for 12 months. Growth, mineral nutrition, and heavy metal content were assessed. Results showed that ectomycorrhizae significantly improved shoot and roots dry masses of P. halepensis seedlings, as well as nitrogen shoot content. The absorption of Pb, Zn, and Cd was much higher in the roots than in the shoots, and significantly more pronounced in ectomycorrhizal seedlings—especially for Zn and Cd. The presence of ectomycorrhizae significantly reduced the translocation factor of Zn and Cd and bioaccumulation factor of Pb and Cd, which enhanced the phytostabilizing potential of P. halepensis seedlings. These results support the use of ectomycorrhizal P. halepensis in the remediation of heavy metal contaminated sites.

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