Comparative analysis of thylakoid protein complexes in the mesophyll and bundle sheath cells from C3, C4and C3–C4Paniceae grasses

2019 ◽  
Vol 166 (1) ◽  
pp. 134-147 ◽  
Author(s):  
Miguel A. Hernández‐Prieto ◽  
Christie Foster ◽  
Alexander Watson‐Lazowski ◽  
Oula Ghannoum ◽  
Min Chen
Keyword(s):  
Comparative Analysis ◽  
Protein Complexes ◽  
Bundle Sheath ◽  
Bundle Sheath Cells ◽  
Thylakoid Protein ◽  
Sheath Cells

scholarly journals The bundle sheath of rice is conditioned to play an active role in water transport as well as sulfur assimilation and jasmonic acid synthesis

2021 ◽  
Author(s):  
Lei Hua ◽  
Sean R. Stevenson ◽  
Ivan Reyna-Llorens ◽  
Haiyan Xiong ◽  
Stanislav Kopriva ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Comparative Analysis ◽  
Jasmonic Acid ◽  
Water Transport ◽  
Acid Synthesis ◽  
Bundle Sheath ◽  
Last Common Ancestor ◽  
Bundle Sheath Cells ◽  
Sheath Cells

Abstract Leaves comprise multiple cell types but our knowledge of the patterns of gene expression that underpin their functional specialization is fragmentary. Our understanding and ability to undertake rational redesign of these cells is therefore limited. We aimed to identify genes associated with the incompletely understood bundle sheath of C3 plants, which represents a key target associated with engineering traits such as C4 photosynthesis into rice. To better understand veins, bundle sheath and mesophyll cells of rice we used laser capture microdissection followed by deep sequencing. Gene expression of the mesophyll is conditioned to allow coenzyme metabolism and redox homeostasis as well as photosynthesis. In contrast, the bundle sheath is specialized in water transport, sulphur assimilation and jasmonic acid biosynthesis. Despite the small chloroplast compartment of bundle sheath cells, substantial photosynthesis gene expression was detected. These patterns of gene expression were not associated with presence/absence of particular transcription factors in each cell type, but rather gradients in expression across the leaf. Comparative analysis with C3Arabidopsis identified a small gene-set preferentially expressed in bundle sheath cells of both species. This included genes encoding transcription factors from fourteen orthogroups, and proteins allowing water transport, sulphate assimilation and jasmonic acid synthesis. The most parsimonious explanation for our findings is that bundle sheath cells from the last common ancestor of rice and Arabidopsis was specialized in this manner, and since the species diverged these patterns of gene expression have been maintained. Significance statement The role of bundle sheath cells in C4 species have been studied intensively but this is not the case in leaves that use the ancestral C3 pathway. Here, we show that gene expression in the bundle sheath of rice is specialized to allow sulphate and nitrate reduction, water transport and jasmonate synthesis, and comparative analysis with Arabidopsis indicates ancient roles for bundle sheath cells in water transport, sulphur and jasmonate synthesis.

Estimation of diffusive resistance of bundle sheath cells to CO2 from modeling of C4 photosynthesis

1996 ◽  
Vol 49 (3) ◽  
pp. 195-208 ◽  
Author(s):  
Dongxiang He ◽  
Gerald E. Edwards
Keyword(s):  
C4 Photosynthesis ◽  
Bundle Sheath ◽  
Diffusive Resistance ◽  
Bundle Sheath Cells ◽  
Sheath Cells

Nitrate metabolism in relation to the aspartate-type C-4 pathway of photosynthesis in Eleusine coracana

1974 ◽  
Vol 52 (12) ◽  
pp. 2599-2605 ◽  
Author(s):  
C. K. M. Rathnam ◽  
V. S. R. Das
Keyword(s):  
Glutamate Dehydrogenase ◽  
Eleusine Coracana ◽  
Bundle Sheath ◽  
Chloroplast Envelope ◽  
Mesophyll Cells ◽  
Bundle Sheath Cells ◽  
Nitrate Metabolism ◽  
Type C ◽  
Envelope Membranes ◽  
Sheath Cells

The intercellular and intracellular distributions of nitrate assimilating enzymes were studied. Nitrate reductase was found to be localized on the chloroplast envelope membranes. The chloroplastic NADPH – glutamate dehydrogenase was concentrated in the mesophyll cells. The extrachloroplastic NADH – glutamate dehydrogenase was localized in the bundle sheath cells. Glutamate synthesized in the mesophyll chloroplasts was interpreted to be utilized exclusively in the synthesis of aspartate, while in the bundle sheath cells it was thought to be consumed in other cellular metabolic processes. Based on the results, a scheme is proposed to account for the nitrate metabolism in the leaves of Eleusine coracana Gaertn. in relation to its aspartate-type C-4 pathway of photosynthesis.

scholarly journals Transient expression from cab-m1 and rbcS-m3 promoter sequences is different in mesophyll and bundle sheath cells in maize leaves.

1992 ◽  
Vol 89 (8) ◽  
pp. 3654-3658 ◽  
Author(s):  
K. C. Bansal ◽  
J. F. Viret ◽  
J. Haley ◽  
B. M. Khan ◽  
R. Schantz ◽  
...  
Keyword(s):  
Transient Expression ◽  
Bundle Sheath ◽  
Promoter Sequences ◽  
Bundle Sheath Cells ◽  
Maize Leaves ◽  
Sheath Cells
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