Transcriptome analysis reveals key genes regulating signaling and metabolic pathways during the growth of moso bamboo ( Phyllostachys edulis ) shoots

2020 ◽  
Author(s):  
Yangang Lan ◽  
Lin Wu ◽  
Min Wu ◽  
Huanlong Liu ◽  
Yameng Gao ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Metabolic Pathways ◽  
Moso Bamboo ◽  
Phyllostachys Edulis ◽  
Key Genes

scholarly journals Transcriptome Reveals the Specificity of Phyllostachys edulis ‘Pachyloen’ Shoots at Different Developmental Stages

Forests ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 861 ◽  
Author(s):  
Yaping Hu ◽  
Ying Zhang ◽  
Jie Zhou ◽  
Guibing Wang ◽  
Qirong Guo
Keyword(s):  
Metabolic Pathways ◽  
Developmental Stages ◽  
Moso Bamboo ◽  
Biological Processes ◽  
Phyllostachys Edulis ◽  
Monthly Basis ◽  
Bamboo Shoots ◽  
Tyrosine Metabolism ◽  
Culm Wall ◽  
Cellular Components

Phyllostachys edulis ‘Pachyloen’ can have a stalk wall thickness of up to 2.5 cm at a height of 1.3 m, which is 1.8 times that of normal Moso bamboo (Phyllostachys edulis); this serves as an excellent cultivar, comprising both wood and bamboo shoots. We collected bamboo shoot samples of Phyllostachys edulis ‘Pachyloen’ and Moso bamboo on a monthly basis from September to April and used transcriptome sequencing to explore the differences in their development. The results showed that there were 666–1839 Phyllostachys edulis ‘Pachyloen’-specific genes at different developmental stages enriched in 20 biological processes, 15 cellular components, 12 molecular functions, and 137 metabolic pathways, 52 of which were significant. Among these, 27 metabolic pathways such as tyrosine metabolism and their uniquely expressed genes were found to play important roles in the thickening of Phyllostachys edulis ‘Pachyloen’. This study provides insights into the mechanisms underlying the thickening of the culm wall of Phyllostachys edulis ‘Pachyloen’.

scholarly journals Transcriptome analysis provides insights into xylogenesis formation in Moso bamboo (Phyllostachys edulis) shoot

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hui Zhang ◽  
Ye-qing Ying ◽  
Jie Wang ◽  
Xian-hai Zhao ◽  
Wei Zeng ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Moso Bamboo ◽  
Phyllostachys Edulis

scholarly journals Main regulatory pathways, key genes and microRNAs involved in flower formation and development of moso bamboo (Phyllostachys edulis)

2016 ◽  
Vol 15 (1) ◽  
pp. 82-96 ◽  
Author(s):  
Wei Ge ◽  
Ying Zhang ◽  
Zhanchao Cheng ◽  
Dan Hou ◽  
Xueping Li ◽  
...  
Keyword(s):  
Moso Bamboo ◽  
Flower Formation ◽  
Phyllostachys Edulis ◽  
Regulatory Pathways ◽  
Key Genes

Transcriptome analysis identifies key genes involved in the regulation of epidermal lupeol biosynthesis in Ricinus communis

2020 ◽  
pp. 113100
Author(s):  
Xiulin Liu ◽  
Rongjun Li ◽  
Wenying Lu ◽  
Zilin Zhou ◽  
Xiaohan Jiang ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Ricinus Communis ◽  
Key Genes

scholarly journals Transcriptome analysis of lateral buds from Phyllostachys edulis rhizome during germination and early shoot stages

2020 ◽  
Author(s):  
Yuting Shou ◽  
Yihua Zhu ◽  
Yulong Ding
Keyword(s):  
Signaling Pathways ◽  
High Throughput Sequencing ◽  
Stem Elongation ◽  
Rapid Development ◽  
Development Stage ◽  
Fast Growth ◽  
Moso Bamboo ◽  
Phyllostachys Edulis ◽  
Lateral Buds ◽  
Meristem Development

Abstract Background: The vegetative growth is an important stage for plants when they conduct photosynthesis, accumulate and collect all resources needed and prepare for reproduction stage. Bamboo is one of the fastest growing plant species. The rapid growth of Phyllostachys edulis results from the expansion of intercalary meristem at the basal part of nodes, which are differentiated from the apical meristem of rhizome lateral buds. However, little is known about the major signaling pathways and players involved during this rapid development stage of bamboo. To study this question, we adopted the high-throughput sequencing technology and compared the transcriptomes of Moso bamboo rhizome buds in germination stage and late development stage. Results: We found that the development of Moso bamboo rhizome lateral buds was coordinated by multiple pathways, including meristem development, sugar metabolism and phytohormone signaling. Phytohormones have fundamental impacts on the plant development. We found the evidence of several major hormones participating in the development of Moso bamboo rhizome lateral bud. Furthermore, we showed direct evidence that Gibberellic Acids (GA) signaling participated in the Moso bamboo stem elongation. Conclusion: Significant changes occur in various signaling pathways during the development of rhizome lateral buds. It is crucial to understand how these changes are translated to Phyllostachys edulis fast growth. These results expand our knowledge on the Moso bamboo internodes fast growth and provide research basis for further study.

Melaleuca alternifolia oil can delay nutrient damage of grapes caused by aspergillus ochraceus through regulation of key genes and metabolites in metabolic pathways

2020 ◽  
Vol 164 ◽  
pp. 111152 ◽  
Author(s):  
Qingjun Kong ◽  
Jianrui Qi ◽  
Peipei An ◽  
Rongrong Deng ◽  
Jiahui Meng ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Aspergillus Ochraceus ◽  
Melaleuca Alternifolia ◽  
Key Genes

scholarly journals Molecular Mechanism of Xylogenesis in Moso Bamboo (Phyllostachys edulis) Shoots during Cold Storage

Polymers ◽  
2018 ◽  
Vol 11 (1) ◽  
pp. 38 ◽  
Author(s):  
Changtao Li ◽  
Lingling Xuan ◽  
Yuming He ◽  
Jie Wang ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cold Storage ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Moso Bamboo ◽  
Transcriptomic Analysis ◽  
Pcr Analysis ◽  
Phyllostachys Edulis ◽  
Related Enzyme ◽  
Cold Chamber ◽  
Xylan Biosynthesis

A bamboo shoot is the immature stem of the woody grass and a nutritious and popular vegetable in East Asia. However, it undergoes a rapid xylogenesis process right after harvest, even being stored in a cold chamber. To investigate the molecular regulation mechanisms of xylogenesis in Moso bamboo (Phyllostachys edulis) shoots (MBSes) during cold storage, the measurement of cell wall polymers (cellulose, hemicellulose, and lignin) and related enzyme activities (phenylalanine ammonia lyase (PAL), cinnamyl alcohol dehydrogenase (CAD), peroxidase (POD), and xylan xylosyltransferase (XylT)) and transcriptomic analysis were performed during cold storage. It was noticed that cellulose and lignin contents increased, while hemicellulose content exhibited a downward trend. PAL, CAD, and POD activity presented an upward trend generally in MBS when stored at 4 °C for 16 days. XylT activity showed a descending trend during the stages of storage, but slightly increased during the 8th to 12th days after harvest at 4 °C. Transcriptomic analysis identified 72, 28, 44, and 31 functional unigenes encoding lignin, cellulose, xylan biosynthesis enzymes, and transcription factors (TFs), respectively. Many of these secondary cell wall (SCW)-related genes showed higher expression levels in the later period of cold storage. Quantitative RT-PCR analysis of the selected genes conformed to the expression pattern. Our study provides a comprehensive analysis of MBS secondary wall biosynthesis at the molecular level during the cold storage process. The results give insight into the xylogenesis process of this economically important vegetable and shed light on solving this problem of the post-harvest industry.

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