Targeting redox metabolism of the maize‐ Azospirillum brasilense interaction exposed to arsenic‐affected groundwater

ABSTRACT The objective of this study was to evaluate the phytomorphological parameters of soybean in a crop-livestock system inoculated with Azospirillum brasilense. The experiment was conducted in Santa Maria, Brazil, for two agricultural years (2 Paper extracted from the doctoral thesis of the first author. 012/2013 and 2013/2014) in a randomized complete block design with three replications. In the winter, the black oat and ryegrass pasture was managed with sheep under different grazing systems: (I) - a conventional grazing (CG) system, where the animals remained in the pasture throughout the experimental period without any pasture height control; (II, III, and IV) - systems with post-grazing pasture heights of 10, 20, and 30 cm, respectively; and (NG) - one control without grazing. All treatments received two nitrogen doses (50 and 100 kg ha-1) and inoculation or no inoculation (A. brasilense). The soybean cultivar BMX Potência RR was sown using no-tillage on 16 November 2012 and 1 December 2013. In each plot, seven rows of plants with spacing of 0.45 m were used, and in four rows, the seeds were inoculated with the bacterium A. brasilense. The phytomorphological variables and grain productivity were evaluated. In a croplivestock system, soybean has better productivity when established on black oat and ryegrass pasture managed with post-grazing pasture heights of between 20 and 30 cm. Areas under conventional grazing in the winter led to smaller soybean plants with lower first and last pod heights. Inoculation with A. brasilense should not be used alone in soybean cultivation.

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Proteasome dysfunction under compromised redox metabolism dictates liver injury in NASH through ASK1/PPARγ binodal complementary modules

Redox Biology ◽

10.1016/j.redox.2021.102043 ◽

2021 ◽

pp. 102043

Author(s):

Debajyoti Das ◽

Avishek Paul ◽

Abhishake Lahiri ◽

Moumita Adak ◽

Sujay K. Maity ◽

...

Keyword(s):

Liver Injury ◽

Redox Metabolism

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Azospirillum brasilense Sp245 triggers cytokinin signaling in root tips and improves biomass accumulation in Arabidopsis through canonical cytokinin receptors

Physiology and Molecular Biology of Plants ◽

10.1007/s12298-021-01036-9 ◽

2021 ◽

Author(s):

Manuel Méndez-Gómez ◽

Elda Castro-Mercado ◽

José López-Bucio ◽

Ernesto García-Pineda

Keyword(s):

Azospirillum Brasilense ◽

Biomass Accumulation ◽

Cytokinin Signaling ◽

Root Tips ◽

Cytokinin Receptors

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Redox metabolism in mussels (Brachidontes solisianus) under the influence of tides in a rocky beach in Southern Brazil

Estuarine Coastal and Shelf Science ◽

10.1016/j.ecss.2021.107424 ◽

2021 ◽

pp. 107424

Author(s):

Daniel C. Moreira ◽

Marcus A.C.T. Sabino ◽

Felipe T.B. Kuzniewski ◽

Orlando V. Furtado-Filho ◽

Juan M. Carvajalino-Fernández ◽

...

Keyword(s):

Southern Brazil ◽

Redox Metabolism

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Expression and function of the cdgD gene, encoding a CHASE–PAS-DGC-EAL domain protein, in Azospirillum brasilense

Scientific Reports ◽

10.1038/s41598-020-80125-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

José Francisco Cruz-Pérez ◽

Roxana Lara-Oueilhe ◽

Cynthia Marcos-Jiménez ◽

Ricardo Cuatlayotl-Olarte ◽

María Luisa Xiqui-Vázquez ◽

...

Keyword(s):

Azospirillum Brasilense ◽

Type Strain ◽

Wild Type Strain ◽

Soil Conditions ◽

Wild Type ◽

Gene Encoding ◽

Wheat Roots ◽

Genes Encoding ◽

In The Wild ◽

Plant Growth Promoting

AbstractThe plant growth-promoting bacterium Azospirillum brasilense contains several genes encoding proteins involved in the biosynthesis and degradation of the second messenger cyclic-di-GMP, which may control key bacterial functions, such as biofilm formation and motility. Here, we analysed the function and expression of the cdgD gene, encoding a multidomain protein that includes GGDEF-EAL domains and CHASE and PAS domains. An insertional cdgD gene mutant was constructed, and analysis of biofilm and extracellular polymeric substance production, as well as the motility phenotype indicated that cdgD encoded a functional diguanylate protein. These results were correlated with a reduced overall cellular concentration of cyclic-di-GMP in the mutant over 48 h compared with that observed in the wild-type strain, which was recovered in the complemented strain. In addition, cdgD gene expression was measured in cells growing under planktonic or biofilm conditions, and differential expression was observed when KNO3 or NH4Cl was added to the minimal medium as a nitrogen source. The transcriptional fusion of the cdgD promoter with the gene encoding the autofluorescent mCherry protein indicated that the cdgD gene was expressed both under abiotic conditions and in association with wheat roots. Reduced colonization of wheat roots was observed for the mutant compared with the wild-type strain grown in the same soil conditions. The Azospirillum-plant association begins with the motility of the bacterium towards the plant rhizosphere followed by the adsorption and adherence of these bacteria to plant roots. Therefore, it is important to study the genes that contribute to this initial interaction of the bacterium with its host plant.

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Expanding the link between circadian rhythms and redox metabolism of epigenetic control

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2021.01.009 ◽

2021 ◽

Author(s):

Tomoki Sato ◽

Carolina Magdalen Greco

Keyword(s):

Circadian Rhythms ◽

Redox Metabolism ◽

Epigenetic Control

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The Hallmarks of Flavonoids in Cancer

Molecules ◽

10.3390/molecules26072029 ◽

2021 ◽

Vol 26 (7) ◽

pp. 2029

Author(s):

Luis Gustavo Saboia Ponte ◽

Isadora Carolina Betim Pavan ◽

Mariana Camargo Silva Mancini ◽

Luiz Guilherme Salvino da Silva ◽

Ana Paula Morelli ◽

...

Keyword(s):

Cell Cycle ◽

Cell Proliferation ◽

Biological Activity ◽

Neurological Disorders ◽

Mechanisms Of Action ◽

Molecular Signaling ◽

Hallmarks Of Cancer ◽

Redox Metabolism ◽

Important Group ◽

Cellular Context

Flavonoids represent an important group of bioactive compounds derived from plant-based foods and beverages with known biological activity in cells. From the modulation of inflammation to the inhibition of cell proliferation, flavonoids have been described as important therapeutic adjuvants against several diseases, including diabetes, arteriosclerosis, neurological disorders, and cancer. Cancer is a complex and multifactor disease that has been studied for years however, its prevention is still one of the best known and efficient factors impacting the epidemiology of the disease. In the molecular and cellular context, some of the mechanisms underlying the oncogenesis and the progression of the disease are understood, known as the hallmarks of cancer. In this text, we review important molecular signaling pathways, including inflammation, immunity, redox metabolism, cell growth, autophagy, apoptosis, and cell cycle, and analyze the known mechanisms of action of flavonoids in cancer. The current literature provides enough evidence supporting that flavonoids may be important adjuvants in cancer therapy, highlighting the importance of healthy and balanced diets to prevent the onset and progression of the disease.

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