Correlation of Serum Vitamin A Levels with Disease Activity Indices and Colonic IL-23R and FOXP3 mRNA Expression in Ulcerative Colitis Patients

Abstract Introduction Assessment of disease activity in Crohn’s disease (CD) and ulcerative colitis (UC) is usually based on the physician’s evaluation of clinical symptoms, endoscopic findings, and biomarker analysis. The German Inflammatory Bowel Disease Activity Index for CD (GIBDICD) and UC (GIBDIUC) uses data from patient-reported questionnaires. It is unclear to what extent the GIBDI agrees with the physicians’ documented activity indices. Methods Data from 2 studies were reanalyzed. In both, gastroenterologists had documented disease activity in UC with the partial Mayo Score (pMS) and in CD with the Harvey Bradshaw Index (HBI). Patient-completed GIBDI questionnaires had also been assessed. The analysis sample consisted of 151 UC and 150 CD patients. Kappa coefficients were determined as agreement measurements. Results Rank correlations were 0.56 (pMS, GIBDIUC) and 0.57 (HBI, GIBDICD), with p < 0.001. The absolute agreement for 2 categories of disease activity (remission yes/no) was 74.2 % (UC) and 76.6 % (CD), and for 4 categories (none/mild/moderate/severe) 60.3 % (UC) and 61.9 % (CD). The kappa values ranged between 0.47 for UC (2 categories) and 0.58 for CD (4 categories). Discussion There is satisfactory agreement of GIBDI with the physician-documented disease activity indices. GIBDI can be used in health care research without access to assessments of medical practitioners. In clinical practice, the index offers a supplementary source of information.

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Vitamin A supplementation decreases disease activity index in patients with ulcerative colitis: A randomized controlled clinical trial

Complementary Therapies in Medicine ◽

10.1016/j.ctim.2018.09.026 ◽

2018 ◽

Vol 41 ◽

pp. 215-219 ◽

Cited By ~ 7

Author(s):

Kourosh Masnadi Shirazi ◽

Zeinab Nikniaz ◽

Arman Masnadi Shirazi ◽

Mohammadreza Rohani

Keyword(s):

Ulcerative Colitis ◽

Clinical Trial ◽

Vitamin A ◽

Disease Activity ◽

Activity Index ◽

Disease Activity Index ◽

Controlled Clinical Trial ◽

Randomized Controlled Clinical Trial ◽

Vitamin A Supplementation ◽

Randomized Controlled

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Obestatin/Ghrelin Ratio as a New Activity Index in Ulcerative Colitis

International Journal of Innovative Research in Medical Science ◽

10.23958/ijirms/vol04-i04/623 ◽

2019 ◽

Vol 4 (04) ◽

Author(s):

Mohammed Amin Mohammed ◽

Nesreen Moustafa Omar

Keyword(s):

Ulcerative Colitis ◽

Disease Activity ◽

Mrna Expression ◽

Activity Index ◽

Strong Relationship ◽

Serum Levels ◽

Ghrelin Level ◽

Mucosal Inflammation ◽

Predictive Values ◽

Serum Ghrelin

Background and Aim: Ulcerative colitis (UC) is an immune-mediated systemic inflammatory process that destroys the intestinal mucosa. Ghrelin, an appetite-regulatory hormone, has anti-inflammatory effects including a decrease in circulating cytokines. Some reports demonstrated a strong relationship between the serum ghrelin level and the severity of mucosal inflammation in the gastrointestinal tract. The aim is to investigate serum levels and colonic mucosal mRNA expression of ghrelin, obestatin, and obestatin/ghrelin ratio in patients with UC and to determine their potential as markers for UC disease activity. Patients and Methods: seventy-five outpatients with UC and 45 age- and sex-matched healthy volunteers were enrolled in this study after written conscious consent and approval by the Institutional Review Board of Mansoura University. UC was diagnosed by conventional clinical, radiological, endoscopic, and histopathological criteria. Serum ghrelin, obestatin levels, and their mucosal mRNA expression were measured by ELISA kits and a real-time quantitative reverse transcriptase polymerase chain reaction according to the manufacturer’s protocols. Results: Serum levels and mucosal mRNA expression of ghrelin were significantly higher in patients with active UC than patients in remission p˂0.0001). Obestatin/ghrelin ratio was significantly lower in patients with active UC (0.26±0.08) than those in remission (0.523±0.16; p˂0.0001). Obestatin/ghrelin ratio was negatively and significantly correlated with inflammation and endoscopic scores, colitis activity index, serum ghrelin level, and its mucosal mRNA expression (p˂0.05). Conclusion: obestatin/ghrelin ratio might be a reliable surrogate non-invasive marker of disease activity in UC with significantly high sensitivity, specificity, predictive values, and diagnostic accuracy.

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Association of serum retinol and circulating inflammatory cells with disease activity in ulcerative colitis patients

Nutrition & Food Science ◽

10.1108/nfs-05-2018-0131 ◽

2019 ◽

Vol 49 (2) ◽

pp. 203-212

Author(s):

Nasim Abedimanesh ◽

Alireza Ostadrahimi ◽

Saeed Abedimanesh ◽

Behrooz Motlagh ◽

Mohammad Hossein Somi

Keyword(s):

Ulcerative Colitis ◽

T Cells ◽

Vitamin A ◽

Disease Activity ◽

Cd8 T Cells ◽

Severe Disease ◽

Inflammatory Cells ◽

Serum Retinol ◽

Content Type ◽

Lymphocyte Ratio

Purpose The purpose of this study is to explore the association of serum retinol and number of circulating inflammatory cells and disease activity in patients with ulcerative colitis. Design/methodology/approach A total of 60 patients with ulcerative colitis were enrolled in a cross-sectional pilot study. Patients were recruited from specialized clinic of Tabriz University of Medical Sciences, Iran between April and August 2015. Mayo clinic index was used to assess clinical disease activity score. Blood samples were collected. Serum retinol was assessed using HPLC to determine vitamin A status. Complete blood count and lymphocyte phenotyping were performed by automated hematology analyzer and flow-cytometric analysis, respectively. Findings According to Mayo scoring, 68.33 per cent of patients had mild and 31.66 per cent had moderate or severe disease activity. About 43.33 per cent of patients were vitamin A deficient, with 23.33 per cent having moderate to severe deficiency (serum retinol < 20 µg/dl). Lower levels of serum retinol and higher count and percentages of CD3+, CD8+ T cells and neutrophil to lymphocyte ratio were statistically associated with disease activity according to univariate analysis (p = 0.002, 0.037, <0.001, 0.031, 0.002 and 0.039); however, in binary logistic regression, only lower levels of serum retinol were independently associated with disease activity with a OR of 0.564 (p = 0.021; 95 per cent CI 0.35-0.92). Originality/value Vitamin A deficiency was detected in this study population. Patients with moderate to severe disease activity demonstrated lower serum retinol, higher CD8+ T cells and neutrophil to lymphocyte ratio compared to patients with mild disease activity.

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Comparing disease activity indices in ulcerative colitis

Journal of Crohn s and Colitis ◽

10.1016/j.crohns.2013.09.010 ◽

2014 ◽

Vol 8 (4) ◽

pp. 318-325 ◽

Cited By ~ 57

Author(s):

A.J. Walsh ◽

A. Ghosh ◽

A.O. Brain ◽

O. Buchel ◽

D. Burger ◽

...

Keyword(s):

Ulcerative Colitis ◽

Disease Activity ◽

Activity Indices

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AB0016 ASSOCIATION OF PTPN22 GENETIC VARIANTS WITH DISEASE SUSCEPTIBILITY AND CLINICAL VARIABLES IN PRIMARY SJÖGREN SYNDROME

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2020-eular.2173 ◽

2020 ◽

Vol 79 (Suppl 1) ◽

pp. 1311.1-1312

Author(s):

P. A. Menchaca Tapia ◽

E. Oregón Romero ◽

D. C. Salazar Camarena ◽

M. Marin Rosales ◽

J. F. Muñoz Valle ◽

...

Keyword(s):

Ulcerative Colitis ◽

Disease Activity ◽

Mrna Expression ◽

Genetic Variants ◽

Sjögren Syndrome ◽

Colorectal Disease ◽

Sjogren Syndrome ◽

Western Mexico ◽

Link Type ◽

Development Risk

Background:Primary Sjögren’s syndrome (pSS) is a systemic autoimmune disease characterized by dysfunction of exocrine glands secondary to lymphocytic infiltration. Lymphoid tyrosine phosphatase (LYP) regulates T and B lymphocyte activation.PTPN22gene encodes LYP; multiple polymorphic variants have been described as genetic risk factor of autoimmune diseases.Objectives:The aim was to analyze thePTPN22rs2488457G>C, rs33996649G>A, and rs2476601C>T genetic variants relationship with the development risk of pSS in the western Mexico population.Methods:One hundred and eighty healthy subjects (HS) and 150 pSS patients, classified according to EULAR 2016 criteria, were included. The genetic variants and mRNA expression were determined through PCR-RFLP and qPCR assays.Results:The frequency of heterozygote rs33996649GA genotype was higher in pSS patients than HS [OR=3.143 (1–10.234), p=0.046], and also, rs33996649GA genotype was associated with high SSDAI score (p=0.01). The pSS patients showed 44-fold more mRNA expression in comparison with HS (p=0.002), and mRNA expression correlates with SSDAI (r2=0.512, p=0.006).Conclusion:The rs33996649G>A genetic variant of thePTPN22gene is associated with increased development risk of pSS in the western Mexican population. The expression mRNA correlates with disease activity in pSS.References:[1]Brito-Zerón, P., Baldini, C., Bootsma, H., Bowman, S. J., Jonsson, R., Mariette, X., Ramos-Casals, M. (2016). Sjögren syndrome.Nature Reviews Disease Primers, 2(July), 1–20.https://doi.org/10.1038/nrdp.2016.47[2]Stanford, S. M., & Bottini, N. (2014). PTPN22: The archetypal non-HLA autoimmunity gene.Nature Reviews Rheumatology,10(10), 602–611.https://doi.org/10.1038/nrrheum.2014.109[3]Chen, Z., Zhang, H., Xia, B., Wang, P., Jiang, T., Song, M., & Wu, J. (2013). Association of PTPN22 gene (rs2488457) polymorphism with ulcerative colitis and high levels of PTPN22 mRNA in ulcerative colitis.International Journal of Colorectal Disease,28(10), 1351–1358.https://doi.org/10.1007/s00384-013-1671-3[4]Machado-Contreras, J. R., Muñoz-Valle, J. F., Cruz, A., Salazar-Camarena, D. C., Marín- Rosales, M., & Palafox-Sánchez, C. A. (2016b). Distribution of PTPN22 polymorphismsin SLE from western Mexico: correlation with mRNA expression and disease activity.Clinical and Experimental Medicine,16(3), 399–406.https://doi.org/10.1007/s10238-015-0359-0Disclosure of Interests:None declared

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Sa1999 Patient Related Outcomes and Disease Activity Indices in New Onset Pediatric Ulcerative Colitis: The PROTECT Study

Gastroenterology ◽

10.1016/s0016-5085(15)31273-7 ◽

2015 ◽

Vol 148 (4) ◽

pp. S-380

Author(s):

Thomas D. Walters ◽

David R. Mack ◽

Brendan Boyle ◽

Anne M. Griffiths ◽

Cary Sauer ◽

...

Keyword(s):

Ulcerative Colitis ◽

Disease Activity ◽

Protect Study ◽

New Onset ◽

Activity Indices

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Optimization of prediction of UC relapse and adjustment of adequate therapy in patient with ulcerative colitiss

South Russian Journal of Therapeutic Practice ◽

10.21886/2712-8156-2020-1-2-65-71 ◽

2020 ◽

Vol 1 (2) ◽

pp. 65-71

Author(s):

A. V. Tkachev ◽

K. E. Mazovka ◽

L. S. Mkrtchyan ◽

A. S. Makarenko ◽

L. T. Takidze

Keyword(s):

Mathematical Model ◽

Ulcerative Colitis ◽

Disease Activity ◽

Colon Mucosa ◽

Adequate Therapy ◽

Clinical Indices ◽

Personalized Approach ◽

Metalloproteinase 9 ◽

Selection Of ◽

Activity Indices

Objective: to improve the assessment of activity of ulcerative colitis and the ability to predict the development of relapse of the disease, as well as the selection of adequate therapy.Materials and methods: the study included 90 people: 70 patients with ulcerative colitis and 20 healthy volunteers. The disease activity was evaluated using 7 disease activity indices. The expression of matrix metalloproteinase -9 (MMP-9) in the colon mucosa was evaluated by immunohistochemistry.Results: data were obtained on the activity of MMP-9 in colonobioptates in patients with ulcerative colitis with varying degrees of disease severity, which complements our knowledge of the pathogenetic mechanisms of UC and, based on the developed mathematical model, allows predicting the development of recurrence of UC. Based on the analysis of clinical indices of UC activity, an algorithm for evaluating the effectiveness of basic therapy has been developed.Conclusion: tools are provided to improve the prognosis of UC relapse, and a personalized approach to evaluating the effectiveness of the alternatives of drug therapy is developed.

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