scholarly journals Field evaluation of specific mycobacterial protein‐based skin test for the differentiation of Mycobacterium bovis‐ infected and bacillus calmette guerin‐vaccinated crossbred cattle in ethiopia

2021 ◽  
Author(s):  
Berecha Bayissa ◽  
Asegedech Sirak ◽  
Aboma Zewude ◽  
Adane Worku ◽  
Balako Gumi ◽  
...  
Keyword(s):  
Skin Test ◽  
Mycobacterium Bovis ◽  
Field Evaluation ◽  
Bacillus Calmette Guerin ◽  
Crossbred Cattle ◽  
Mycobacterial Protein

scholarly journals Field evaluation of Specific Mycobacterial Proteins-Based Skin Test for the Differentiation of Mycobacterium bovis-Infected and Bacillus Calmette Guerin-Vaccinated Crossbred Cattle in Ethiopia

2021 ◽  
Author(s):  
Berecha Bayissa ◽  
Aboma Zewude ◽  
Adane Worku ◽  
Balako Gumi ◽  
Stefan Berg ◽  
...  
Keyword(s):  
Skin Test ◽  
Mycobacterium Bovis ◽  
Control Strategy ◽  
Bovine Tuberculosis ◽  
Field Evaluation ◽  
Dairy Production ◽  
Bacillus Calmette Guerin ◽  
Crossbred Cattle ◽  
Test Reactions ◽  
Higher Sensitivity

Bovine tuberculosis (bTB) challenges intensive dairy production in Ethiopia and implementation of the test and slaughter control strategy is not economically acceptable in the country. Vaccination of cattle with Bacillus Calmette-Guerin (BCG) could be an important adjunct to control, which would require a diagnostic test to differentiate Mycobacterium bovis (M. bovis)-infected and BCG-vaccinated animals (DIVA role). This study describes evaluation of a DIVA skin test (DST) that is based on a cocktail (DSTc) or fusion (DSTf) of specific (ESAT-6, CFP-10 and Rv3615c) M. bovis proteins in Zebu-Holstein crossbred cattle in Ethiopia. The study animals used were 74 calves (35 BCG-vaccinated and 39 unvaccinated) aged less than three weeks at the start and 68 known bTB positive cows. Six weeks after vaccination, the 74 calves were tested with DSTc and the single intradermal cervical comparative tuberculin (SICCT) test. The cows were tested with DSTc and SICCT test. Reactions to DSTc were not observed in BCG-vaccinated and unvaccinated calves while SICCT test reactions were detected in vaccinated calves. DSTc reactions were detected in 95.6% of the cows and single intradermal tuberculin (SIT) positive reactions were found in 98.2% (95% confidence interval, CI, 92.1–100%). The sensitivity of DSTc was 95.6% (95% CI, 87.6–99.1%), and significantly (P<0.001) higher than the sensitivity (75%, 95% CI, 63.0-84.7%) of the SICCT test at 4mm cutoff. DSTf and DSTc reactions were correlated (r = 0.75; 95% CI =0.53–0.88). In conclusion, DSTc could differentiate M. bovis-infected from BCG-vaccinated cattle in Ethiopia. DST had higher sensitivity than the SICCT test. Hence, DSTc could be used as a diagnostic tool for bTB if BCG vaccination is implemented for the control of bTB in Ethiopia and other countries.

scholarly journals Improved Skin Test for Differential Diagnosis of Bovine Tuberculosis by the Addition of Rv3020c-Derived Peptides

2012 ◽  
Vol 19 (4) ◽  
pp. 620-622 ◽  
Author(s):  
Gareth J. Jones ◽  
Adam Whelan ◽  
Derek Clifford ◽  
Mick Coad ◽  
H. Martin Vordermeier
Keyword(s):  
Differential Diagnosis ◽  
Skin Test ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Johne's Disease ◽  
Diagnostic Sensitivity ◽  
Bacillus Calmette Guerin ◽  
Content Type ◽  
The Face ◽  
Mycobacterial Antigens

ABSTRACTA peptide cocktail derived from the mycobacterial antigens ESAT-6, CFP-10, and Rv3615c allowed differentiation betweenMycobacterium bovis-infected and M. bovis bacillus Calmette-Guérin (BCG)-vaccinated cattle when used as a skin test reagent for a “DIVA” test (i.e., a test capable ofdifferentiatinginfected and uninfectedvaccinatedanimals). Addition of the antigen Rv3020c improves the diagnostic sensitivity without compromising specificity in the face of BCG or Johne's disease vaccination.

scholarly journals Field Evaluation of a Novel Differential Diagnostic Reagent for Detection of Mycobacterium bovis in Cattle

2006 ◽  
Vol 13 (10) ◽  
pp. 1119-1124 ◽  
Author(s):  
P. J. Cockle ◽  
S. V. Gordon ◽  
R. G. Hewinson ◽  
H. M. Vordermeier
Keyword(s):  
Skin Test ◽  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Field Evaluation ◽  
Current Lead ◽  
Infected Cattle ◽  
Bcg Vaccination ◽  
Diagnostic Reagent ◽  
Better Than

ABSTRACT In the search for improved tools with which to control bovine tuberculosis, the development of enhanced immunodiagnostic reagents is a high priority. Such reagents are required to improve the performance of tuberculin-based reagents and allow the discrimination of vaccinated cattle from those infected with Mycobacterium bovis. In this study, we identified the immunodominant, frequently recognized peptides from Rv3873, Rv3879c, Rv0288, and Rv3019c, which, together with peptides comprising the current lead diagnostic antigens, ESAT-6 and CFP-10, were formulated into a peptide cocktail. In a test of naturally infected cattle, this cocktail was significantly better than tuberculin was for identifying skin test-negative animals with confirmed bovine tuberculosis. In addition, the specificity of this cocktail was not compromised by Mycobacterium bovis BCG vaccination. In summary, our results prioritize this peptide-based, fully synthetic reagent for assessment in larger trials.

Mycobacterium bovis Bacillus Calmette-Guérin Killed by Extended Freeze-Drying Reduces Colitis in Mice

2011 ◽  
Vol 141 (2) ◽  
pp. 642-652.e4 ◽  
Author(s):  
Micheline Lagranderie ◽  
Christoph Kluge ◽  
Helene Kiefer–Biasizzo ◽  
Mohammad Abolhassani ◽  
Marie–Anne Nahori ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Freeze Drying ◽  
Bacillus Calmette Guerin

scholarly journals Impact of in vitro evolution on antigenic diversity of Mycobacterium bovis bacillus Calmette-Guerin (BCG)

Vaccine ◽  
2014 ◽  
Vol 32 (45) ◽  
pp. 5998-6004 ◽  
Author(s):  
Richard Copin ◽  
Mireia Coscollá ◽  
Efstratios Efstathiadis ◽  
Sebastien Gagneux ◽  
Joel D. Ernst
Keyword(s):  
Mycobacterium Bovis ◽  
Bacillus Calmette Guerin ◽  
In Vitro Evolution ◽  
Antigenic Diversity

scholarly journals The double-sided effects of Mycobacterium Bovis bacillus Calmette–Guérin vaccine

npj Vaccines ◽  
2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Junli Li ◽  
Lingjun Zhan ◽  
Chuan Qin
Keyword(s):  
Literature Review ◽  
Experimental Evidence ◽  
Mycobacterium Bovis ◽  
Virus Disease ◽  
Bcg Vaccine ◽  
Bacillus Calmette Guerin ◽  
Mycobacterial Infections ◽  
Specific Effects ◽  
Mycobacterial Diseases ◽  
Protection Mechanisms

AbstractBacillus Calmette–Guérin (BCG), the only vaccine proven to be effective against tuberculosis (TB), is the most commonly used vaccine globally. In addition to its effects on mycobacterial diseases, an increasing amount of epidemiological and experimental evidence accumulated since its introduction in 1921 has shown that BCG also exerts non-specific effects against a number of diseases, such as non-mycobacterial infections, allergies and certain malignancies. Recent Corona Virus Disease 2019 (COVID-19) outbreak has put BCG, a classic vaccine with significant non-specific protection, into the spotlight again. This literature review briefly covers the diverse facets of BCG vaccine, providing new perspectives in terms of specific and non-specific protection mechanisms of this old, multifaceted, and controversial vaccine.

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