Mass spectrometry‐based quantification and spatial location of small organic acids exudate in plant roots under phosphorous deficiency and aluminium toxicity

2021 ◽  
Author(s):  
David Gomez‐Zepeda ◽  
Moises Frausto ◽  
Héctor‐Rogelio Nájera‐González ◽  
Luis Herrera‐Estrella ◽  
José‐Juan Ordaz‐Ortiz
Keyword(s):  
Mass Spectrometry ◽  
Organic Acids ◽  
Spatial Location ◽  
Aluminium Toxicity ◽  
Plant Roots

Application of Derivatization Gas Chromatography/Mass Spectrometry for the Identification and Quantitation of Pinitol in Plant Roots

2009 ◽  
Vol 42 (13) ◽  
pp. 2096-2105 ◽  
Author(s):  
Shaun Garland ◽  
Steven Goheen ◽  
Pat Donald ◽  
Luther McDonald ◽  
James Campbell
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Plant Roots ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

scholarly journals National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines: Follow-Up Testing for Metabolic Disease Identified by Expanded Newborn Screening Using Tandem Mass Spectrometry; Executive Summary

2009 ◽  
Vol 55 (9) ◽  
pp. 1615-1626 ◽  
Author(s):  
Dennis J Dietzen ◽  
Piero Rinaldo ◽  
Ronald J Whitley ◽  
William J Rhead ◽  
W Harry Hannon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Tandem Mass Spectrometry ◽  
Organic Acids ◽  
Newborn Screening ◽  
Tandem Mass ◽  
Confirmatory Testing ◽  
The Us ◽  
The Impact

Abstract Background: Almost all newborns in the US are screened at birth for multiple inborn errors of metabolism using tandem mass spectrometry. Screening tests are designed to be sufficiently sensitive so that cases are not missed. The NACB recognized a need for standard guidelines for laboratory confirmation of a positive newborn screen such that all babies would benefit from equal and optimal follow-up by confirmatory testing. Methods: A committee was formed to review available data pertaining to confirmatory testing. The committee evaluated previously published guidelines, published methodological and clinical studies, clinical case reports, and expert opinion to support optimal confirmatory testing. Grading was based on guidelines adopted from criteria derived from the US Preventive Services Task Force and on the strength of recommendations and the quality of the evidence. Three primary methods of analyte measurement were evaluated for confirmatory testing including measurement of amino acids, organic acids, and carnitine esters. The committee graded the evidence for diagnostic utility of each test for the screened conditions. Results: Ample data and experience were available to make strong recommendations for the practice of analyzing amino acids, organic acids, and acylcarnitines. Likewise, strong recommendations were made for the follow-up test menu for many disorders, particularly those with highest prevalence. Fewer data exist to determine the impact of newborn screening on patient outcomes in all but a few disorders. The guidelines also provide an assessment of developing technology that will fuel a refinement of current practice and ultimate expansion of the diseases detectable by tandem mass spectrometry. Conclusions: Guidelines are provided for optimal follow-up testing for positive newborn screens using tandem mass spectrometry. The committee regards these tests as reliable and currently optimal for follow-up testing. .

Determination of Organic Acids in Honey by Liquid Chromatography with Tandem Mass Spectrometry

2020 ◽  
Vol 13 (12) ◽  
pp. 2249-2257
Author(s):  
Momoka Suto ◽  
Hiroto Kawashima ◽  
Yasunori Nakamura
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Organic Acids ◽  
Tandem Mass

scholarly journals An enhanced procedure for measuring organic acids and methyl esters in PM<sub>2.5</sub>

2015 ◽  
Vol 8 (3) ◽  
pp. 2379-2407
Author(s):  
F. Liu ◽  
F. Duan ◽  
K. He ◽  
Y. Ma ◽  
K. A. Rahn ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Organic Acids ◽  
Carboxylic Acids ◽  
Solid Phase ◽  
Methyl Esters ◽  
Aromatic Acids ◽  
Naphthalic Anhydride ◽  
Aliphatic Acids ◽  
Specificity And Sensitivity

Abstract. We have developed an enhanced analytical procedure to measure organic acids and methyl esters in fine aerosol with much greater specificity and sensitivity than previously available. This capability is important because of these species and their low concentrations, even in highly polluted atmospheres like Beijing, China. The procedure first separates the acids and esters from the other organic compounds with anion-exchange solid- phase extraction (SPE), then, quantifies them by gas chromatography coupled with mass spectrometry. This allows us to accurately quantify the C4-C11 dicarboxylic and the C8-C30 monocarboxylic acids. Then the acids are separated from the esters on an aminopropyl SPE cartridge, whose weak retention isolates and enriches the acids from esters prevents the fatty acids and dimethyl phthalate from being overestimated. The resulting correlations between the aliphatic acids and fatty acid methyl esters (FAMEs) suggest that FAMEs had sources similar to those of the carboxylic acids, or were formed by esterifying carboxylic acids, or that aliphatic acids were formed by hydrolyzing FAMEs. In all, 17 aromatic acids were identified and quantified using this procedure coupled with gas chromatography-tandem mass spectrometry, including the five polycyclic aromatic hydrocarbon (PAH) acids 2-naphthoic, biphenyl-4-carboxylic, 9-oxo-9H-fluorene-1-carboxylic, biphenyl-4,4´-dicarboxylic, and phenanthrene-1-carboxylic acid, plus 1,8-naphthalic anhydride. Correlations between the PAH-acids and the dicarboxylic and aromatic acids indicated that the first three acids and 1,8-naphthalic anhydride were mainly secondary, the last two mainly primary.

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