The intragenic suppressor mutation Leu59Phe compensates for the effect of detrimental mutations in the jasmonate receptor COI1

2021 ◽  
Author(s):  
Susheng Song ◽  
Bei Liu ◽  
Jiaqi Zhai ◽  
Yue Zhang ◽  
Kai Wang ◽  
...  
Keyword(s):  
Suppressor Mutation ◽  
Intragenic Suppressor

Analysis of Porphyromonas gingivalis PG27 by deletion and intragenic suppressor mutation analyses

2011 ◽  
Vol 26 (5) ◽  
pp. 321-335 ◽  
Author(s):  
I. Ishiguro ◽  
K. Saiki ◽  
K. Konishi
Keyword(s):  
Porphyromonas Gingivalis ◽  
Suppressor Mutation ◽  
Mutation Analyses ◽  
Intragenic Suppressor

Ein pseudoallel-spezifischer Suppressor bei Salmonella typhimurium

1956 ◽  
Vol 11 (6) ◽  
pp. 317-329 ◽  
Author(s):  
P. Starlinger ◽  
F. Kaudewitz
Keyword(s):  
Salmonella Typhimurium ◽  
Uv Irradiation ◽  
Genetic Material ◽  
Suppressor Gene ◽  
Growth Characteristics ◽  
Suppressor Mutation ◽  
Deficient Mutant ◽  
Colony Type ◽  
Gene Concept ◽  
Small Colony

In Salmonella typhimurium, UV-irradiation of the histidine-deficient mutant hi-31 induced a slowly growing reversion to histidine-independence. This formed colonies much smaller than wildtype. By means of transduction the newly created character “small colony” was proved to be heritable. As demonstrated by the following experiments this was due to an independant suppressor-mutation (S-31). After UV-irradiation of hi-31/S-31 a small fraction of cells forming large colonies, revealed growth-characteristics of wildtype. After transduction of small colony type cells by phages raised on wildtype the same large colonies were obtained. Expected to be of genotype hi-31+/S-31, they were used as donor for transduction of hi-31. Both, large colonies (hi-31+/S-31) and small colonies (hi-31/S-31) were isolated, which proved to be stable in further transfers.26 histidin-requiring mutants, belonging to 4 groups, each of them characterised by the same block in histidine synthesis, were transduced with phage raised on hi-31/S-31. None of them exhibited changes of growth characteristics, resulting from the incorporation of the suppressor-gene. Thus the suppressor S-31 turned out to be pseudoallel-specific.This result is considered as evidence that pseudoalleles of the histidine series in Salmonella typhimurium not only are units of recombination but also of function of genetic material. The differences between levels of functions exhibited by units which have been called pseudoalleles by various investigators are discussed in connection with the gene concept.

scholarly journals Interdependent YpsA- and YfhS-Mediated Cell Division and Cell Size Phenotypes in Bacillus subtilis

mSphere ◽  
2020 ◽  
Vol 5 (4) ◽  
Author(s):  
Robert S. Brzozowski ◽  
Brooke R. Tomlinson ◽  
Michael D. Sacco ◽  
Judy J. Chen ◽  
Anika N. Ali ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Cell Division ◽  
Cell Size ◽  
Unknown Function ◽  
Suppressor Mutation ◽  
Gram Positive ◽  
Content Type ◽  
Suppressor Mutations ◽  
Extragenic Suppressor ◽  
Cell Division Inhibition

ABSTRACT Although many bacterial cell division factors have been uncovered over the years, evidence from recent studies points to the existence of yet-to-be-discovered factors involved in cell division regulation. Thus, it is important to identify factors and conditions that regulate cell division to obtain a better understanding of this fundamental biological process. We recently reported that in the Gram-positive organisms Bacillus subtilis and Staphylococcus aureus, increased production of YpsA resulted in cell division inhibition. In this study, we isolated spontaneous suppressor mutations to uncover critical residues of YpsA and the pathways through which YpsA may exert its function. Using this technique, we were able to isolate four unique intragenic suppressor mutations in ypsA (E55D, P79L, R111P, and G132E) that rendered the mutated YpsA nontoxic upon overproduction. We also isolated an extragenic suppressor mutation in yfhS, a gene that encodes a protein of unknown function. Subsequent analysis confirmed that cells lacking yfhS were unable to undergo filamentation in response to YpsA overproduction. We also serendipitously discovered that YfhS may play a role in cell size regulation. Finally, we provide evidence showing a mechanistic link between YpsA and YfhS. IMPORTANCE Bacillus subtilis is a rod-shaped Gram-positive model organism. The factors fundamental to the maintenance of cell shape and cell division are of major interest. We show that increased expression of ypsA results in cell division inhibition and impairment of colony formation on solid medium. Colonies that do arise possess compensatory suppressor mutations. We have isolated multiple intragenic (within ypsA) mutants and an extragenic suppressor mutant. Further analysis of the extragenic suppressor mutation led to a protein of unknown function, YfhS, which appears to play a role in regulating cell size. In addition to confirming that the cell division phenotype associated with YpsA is disrupted in a yfhS-null strain, we also discovered that the cell size phenotype of the yfhS knockout mutant is abolished in a strain that also lacks ypsA. This highlights a potential mechanistic link between these two proteins; however, the underlying molecular mechanism remains to be elucidated.

A Suppressor Mutation Which Suppresses Adenylyl Cyclase Mutations in Neurospora crassa

1995 ◽  
Vol 19 (4) ◽  
pp. 320-323 ◽  
Author(s):  
Tadako Murayama ◽  
Yasuyuki Fujisawa ◽  
Yoko Okano
Keyword(s):  
Neurospora Crassa ◽  
Adenylyl Cyclase ◽  
Suppressor Mutation

Accumulation of U14 small nuclear RNA in Saccharomyces cerevisiae requires box C, box D, and a 5', 3' terminal stem

1992 ◽  
Vol 12 (10) ◽  
pp. 4456-4463
Author(s):  
G M Huang ◽  
A Jarmolowski ◽  
J C Struck ◽  
M J Fournier
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Binding ◽  
Essential Elements ◽  
Structural Elements ◽  
Small Nuclear Rna ◽  
Small Nuclear Rnas ◽  
Nuclear Rna ◽  
Compensatory Base Changes ◽  
Normal Processing ◽  
Intragenic Suppressor

U14 is one of several nucleolar small nuclear RNAs required for normal processing of rRNA. Functional mapping of U14 from Saccharomyces cerevisiae has yielded a number of mutants defective in U14 accumulation or function. In this study, we have further defined three structural elements required for U14 accumulation. The essential elements include the U14-conserved box C and box D sequences and a 5', 3' terminal stem. The box elements are coconserved among several nucleolar small nuclear RNAs and have been implicated in binding of the protein fibrillarin. New mutational results show that the first GA bases of the box C sequence UGAUGA are essential, and two vital bases in box D have also been identified. An intragenic suppressor of a lethal box C mutant has been isolated and shown to contain a new box C-like PyGAUG sequence two bases upstream of normal box C. The importance of the terminal stem was confirmed from new compensatory base changes and the finding that accumulation defects in the box elements can be complemented by extending the terminal stem. The results suggest that the observed defects in accumulation reflect U14 instability and that protein binding to one or more of these elements is required for metabolic stability.

scholarly journals Capped mRNA Degradation Intermediates Accumulate in the Yeast spb8-2 Mutant

1998 ◽  
Vol 18 (9) ◽  
pp. 5062-5072 ◽  
Author(s):  
Ronald Boeck ◽  
Bruno Lapeyre ◽  
Christine E. Brown ◽  
Alan B. Sachs
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Family Member ◽  
Gene Deletion ◽  
Binding Protein ◽  
Mrna Degradation ◽  
Protein Family ◽  
Suppressor Mutation ◽  
Mrna Decapping ◽  
Mrna Species ◽  
Degradation Intermediates

ABSTRACT mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8(YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that thespb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3′ end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3′-5′ mRNA degradation intermediates in yeast.

Effects of the Escherichia coli recF suppressor mutation, recA801, on recF-dependent DNA-repair associated phenomena

1987 ◽  
Vol 184 (3) ◽  
pp. 181-186 ◽  
Author(s):  
Michael R. Volkert ◽  
Margaret A. Hartke
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Suppressor Mutation

Enhancement of the Enzymatic Activity of Ribonuclease HI fromThermus thermophilusHB8 with a Suppressor Mutation Method†

Biochemistry ◽  
2000 ◽  
Vol 39 (43) ◽  
pp. 13285-13294 ◽  
Author(s):  
Nobutaka Hirano ◽  
Mitsuru Haruki ◽  
Masaaki Morikawa ◽  
Shigenori Kanaya
Keyword(s):  
Enzymatic Activity ◽  
Suppressor Mutation
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