Inter‐ and intraobserver agreement for CT measurement of mandibular and medial retropharyngeal lymph nodes is excellent in dogs with histologically confirmed oral melanoma

A malignant dendritic melanoma of the oral cavity with metastases widely spread in a cow is described. The clinicopathological manifestations, developed during a two-year period, was characterized by the difficulty in mastication and swallowing, progressive weight loss, weakness and profuse sialorrhea. At the necropsy, an ulcerated black mass was found at the left jaw projecting into the oral cavity. The tumor reached the chin, inferior lip and tongue. Metastases were detected within the tongue, parotid gland, lymph nodes, trachea, thyroid, pleura, lungs, heart, peritoneum, omentum, and serosal membranes of the forestomach and abomasum, liver, and intima of some hepatic vessels, mammary gland, and muscles and fasciae of the left hind leg. No reference to metastasizing oral dendritic melanoma in the oral cavity of the cattle has been mentioned in the literature.

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Scanning electron microscopy of freeze-fractured prescapular lymph node of caprine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111331 ◽

1984 ◽

Vol 42 ◽

pp. 244-245

Author(s):

O. Faroon ◽

F. Al-Bagdadi ◽

T. G. Snider ◽

C. Titkemeyer

Keyword(s):

Lymph Node ◽

Lymph Nodes ◽

Lymphatic System ◽

Three Dimensional ◽

Meat Products ◽

The Body ◽

Morphological Data ◽

The World ◽

Cellular Constituent ◽

Scanning Electron

The lymphatic system is very important in the immunological activities of the body. Clinicians confirm the diagnosis of infectious diseases by palpating the involved cutaneous lymph node for changes in size, heat, and consistency. Clinical pathologists diagnose systemic diseases through biopsies of superficial lymph nodes. In many parts of the world the goat is considered as an important source of milk and meat products.The lymphatic system has been studied extensively. These studies lack precise information on the natural morphology of the lymph nodes and their vascular and cellular constituent. This is due to using improper technique for such studies. A few studies used the SEM, conducted by cutting the lymph node with a blade. The morphological data collected by this method are artificial and do not reflect the normal three dimensional surface of the examined area of the lymph node. SEM has been used to study the lymph vessels and lymph nodes of different animals. No information on the cutaneous lymph nodes of the goat has ever been collected using the scanning electron microscope.

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Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141743 ◽

1995 ◽

Vol 53 ◽

pp. 1074-1075

Author(s):

F.E. Hossler ◽

M.I. McKamey ◽

F.C. Monson

Keyword(s):

Lymph Nodes ◽

Light Microscopy ◽

Corrosion Casting ◽

Fixed Tissue ◽

Infusion Pressure ◽

India Ink ◽

Cacodylate Buffer ◽

Rabbit Bladder ◽

Lateral Walls ◽

Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

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