Expression profile of human porcine endogenous retrovirus A receptors ( HuPAR‐1, HuPAR‐2 ) and transcription factor activator protein‐2γ ( TFAP‐2C ) genes in infected human fibroblasts—Model in vitro

2019 ◽  
Vol 26 (6) ◽  
Author(s):  
Emilia Wojdas ◽  
Krzysztof Łopata ◽  
Roman Nowak ◽  
Magdalena Kimsa‐Dudek ◽  
Paweł Łopata ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Expression Profile ◽  
Human Fibroblasts ◽  
Endogenous Retrovirus ◽  
Activator Protein ◽  
Porcine Endogenous Retrovirus

Expression profile of Tripartite motif family (TRIM) in human fibroblasts (NHDF) infected with porcine endogenous retrovirus (PERV)

2020 ◽  
Author(s):  
Morawiec Emilia ◽  
Nowak Roman ◽  
Strzałka‐Mrozik Barbara ◽  
Mazurek Urszula ◽  
Łopata Krzysztof ◽  
...  
Keyword(s):  
Expression Profile ◽  
Human Fibroblasts ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus ◽  
Tripartite Motif

scholarly journals Identification of Exogenous Forms of Human-Tropic Porcine Endogenous Retrovirus in Miniature Swine

2004 ◽  
Vol 78 (5) ◽  
pp. 2494-2501 ◽  
Author(s):  
James C. Wood ◽  
Gary Quinn ◽  
Kristen M. Suling ◽  
Beth A. Oldmixon ◽  
Brian A. Van Tine ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Germ Line ◽  
Endogenous Retrovirus ◽  
Human Cells ◽  
Miniature Swine ◽  
Peripheral Blood Mononuclear ◽  
Infectious Risk ◽  
Porcine Endogenous Retrovirus ◽  
Principal Source

ABSTRACT The replication of porcine endogenous retrovirus subgroup A (PERV-A) and PERV-B in certain human cell lines indicates that PERV may pose an infectious risk in clinical xenotransplantation. We have previously reported that human-tropic PERVs isolated from infected human cells following cocultivation with miniature swine peripheral blood mononuclear cells (PBMC) are recombinants of PERV-A with PERV-C. Here, we report that these recombinants are exogenous viruses in miniature swine; i.e., they are not present in the germ line DNA. These viruses were invariably present in miniature swine that transmitted PERV to human cells and were also identified in some miniature swine that lacked this ability. These data, together with the demonstration of the absence of both replication-competent PERV-A and recombinant PERV-A/C loci in the genome of miniature swine (L. Scobie, S. Taylor, J. C. Wood, K. M. Suling, G. Quinn, C. Patience, H.-J. Schuurman, and D. E. Onions, J. Virol. 78:2502-2509, 2004), indicate that exogenous PERV is the principal source of human-tropic virus in these animals. Interestingly, strong expression of PERV-C in PBMC correlated with an ability of the PBMC to transmit PERV-A/C recombinants in vitro, indicating that PERV-C may be an important factor affecting the production of human-tropic PERV. In light of these observations, the safety of clinical xenotransplantation from miniature swine will be most enhanced by the utilization of source animals that do not transmit PERV to either human or porcine cells. Such animals were identified within the miniature swine herd and may further enhance the safety of clinical xenotransplantation.

scholarly journals Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-17 ◽  
Author(s):  
Sabine Conrad ◽  
Hossein Azizi ◽  
Maryam Hatami ◽  
Mikael Kubista ◽  
Michael Bonin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Adult Stem Cells ◽  
Single Cells ◽  
Human Fibroblasts ◽  
Embryonic Stem ◽  
Human Adult

The aim of this study was to elucidate the molecular status of single human adult germ stem cells (haGSCs) and haGSC colonies, which spontaneously developed from the CD49f MACS and matrix- (collagen−/laminin+ binding-) selected fraction of enriched spermatogonia. Single-cell transcriptional profiling by Fluidigm BioMark system of a long-term cultured haGSCs cluster in comparison to human embryonic stem cells (hESCs) and human fibroblasts (hFibs) revealed that haGSCs showed a characteristic germ- and pluripotency-associated gene expression profile with some similarities to hESCs and with a significant distinction from somatic hFibs. Genome-wide comparisons with microarray analysis confirmed that different haGSC colonies exhibited gene expression heterogeneity with more or less pluripotency. The results of this study confirm that haGSCs are adult stem cells with a specific molecular gene expression profilein vitro, related but not identical to true pluripotent stem cells. Under ES-cell conditions haGSC colonies could be selected and maintained in a partial pluripotent state at the molecular level, which may be related to their cell plasticity and potential to differentiate into cells of all germ layers.

scholarly journals Extended Analysis of the In Vitro Tropism of Porcine Endogenous Retrovirus

2000 ◽  
Vol 74 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Carolyn A. Wilson ◽  
Susan Wong ◽  
Matthew VanBrocklin ◽  
Mark J. Federspiel
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Line ◽  
Endogenous Retrovirus ◽  
Human Cells ◽  
Productive Infection ◽  
Porcine Endogenous Retrovirus ◽  
Productive Replication

ABSTRACT We previously reported that mitogenic activation of porcine peripheral blood mononuclear cells resulted in production of porcine endogenous retrovirus(es) (PERV[s]) capable of productively infecting human cells (C. Wilson et al., J. Virol. 72:3082–3087, 1998). We now extend that analysis to show that additional passage of isolated virus, named here PERV-NIH, through a human cell line yielded a viral population with a higher titer of infectious virus on human cells than the initial isolate. We show that in a single additional passage on a human cell line, the increase in infectivity for human cells is accounted for by selection against variants carrying pig-tropic envelope sequences (PERV-C) as well as by enrichment for replication-competent genomes. Sequence analysis of the envelope cDNA present in virions demonstrated that the envelope sequence of PERV-NIH is related to but distinct from previously reported PERV envelopes. The in vitro host range of PERV was studied in human primary cells and cell lines, as well as in cell lines from nonhuman primate and other species. This analysis reveals three patterns of susceptibility to infection among these host cells: (i) cells are resistant to infection in our assay; (ii) cells are infected by virus, as viral RNA is detected in the supernatant by reverse transcription-PCR, but the cells are not permissive to productive replication and spread; and (iii) cells are permissive to low-level productive replication. Certain cell lines were permissive for efficient productive infection and spread. These results may prove useful in designing appropriate animal models to assess the in vivo infectivity properties of PERV.

scholarly journals Celecoxib as a Valuable Adjuvant in Cutaneous Melanoma Treated with Trametinib

2021 ◽  
Vol 22 (9) ◽  
pp. 4387
Author(s):  
Diana Valentina Tudor ◽  
Ioana Bâldea ◽  
Diana Elena Olteanu ◽  
Eva Fischer-Fodor ◽  
Virag Piroska ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Protein Kinase ◽  
Mapk Pathway ◽  
Human Fibroblasts ◽  
In Vitro Study ◽  
Melanoma Cells ◽  
Mitogen Activated Protein Kinase ◽  
Culture Cells ◽  
Nm Range

Background: Melanoma patients stop responding to targeted therapies mainly due to mitogen activated protein kinase (MAPK) pathway re-activation, phosphoinositide 3 kinase/the mechanistic target of rapamycin (PI3K/mTOR) pathway activation or stromal cell influence. The future of melanoma treatment lies in combinational approaches. To address this, our in vitro study evaluated if lower concentrations of Celecoxib (IC50 in nM range) could still preserve the chemopreventive effect on melanoma cells treated with trametinib. Materials and Methods: All experiments were conducted on SK-MEL-28 human melanoma cells and BJ human fibroblasts, used as co-culture. Co-culture cells were subjected to a celecoxib and trametinib drug combination for 72 h. We focused on the evaluation of cell death mechanisms, melanogenesis, angiogenesis, inflammation and resistance pathways. Results: Low-dose celecoxib significantly enhanced the melanoma response to trametinib. The therapeutic combination reduced nuclear transcription factor (NF)–kB (p < 0.0001) and caspase-8/caspase-3 activation (p < 0.0001), inhibited microphthalmia transcription factor (MITF) and tyrosinase (p < 0.05) expression and strongly down-regulated the phosphatidylinositol-3-kinase/protein kinase B (PI3K/AKT) signaling pathway more significantly than the control or trametinib group (p < 0.0001). Conclusion: Low concentrations of celecoxib (IC50 in nM range) sufficed to exert antineoplastic capabilities and enhanced the therapeutic response of metastatic melanoma treated with trametinib.

No evidence of in vitro and in vivo porcine endogenous retrovirus infection after plasmapheresis through the AMC-bioartificial liver

2005 ◽  
Vol 12 (4) ◽  
pp. 286-292 ◽  
Author(s):  
Giuseppe Di Nicuolo ◽  
Maarten-Paul Kerkhove ◽  
Ruurdtje Hoekstra ◽  
Marcel G. H. M. Beld ◽  
Pietro Amoroso ◽  
...  
Keyword(s):  
Endogenous Retrovirus ◽  
Bioartificial Liver ◽  
Porcine Endogenous Retrovirus ◽  
Retrovirus Infection

Porcine endogenous retrovirus is transmitted neither in vivo nor in vitro from porcine endothelial cells to baboons

1999 ◽  
Vol 31 (1-2) ◽  
pp. 913-914 ◽  
Author(s):  
U Martin ◽  
G Steinhoff ◽  
V Kiessig ◽  
M Chikobava ◽  
M Anssar ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus ◽  
Porcine Endothelial Cells

scholarly journals Detection of Pig Cells Harboring Porcine Endogenous Retroviruses in Non-Human Primate Bladder After Renal Xenotransplantation

Viruses ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 801 ◽  
Author(s):  
Yoonki Heo ◽  
Yeondong Cho ◽  
Keon Bong Oh ◽  
Ki Hoon Park ◽  
Hansam Cho ◽  
...  
Keyword(s):  
Endogenous Retrovirus ◽  
Endogenous Retroviruses ◽  
Inverse Pcr ◽  
Transgenic Pigs ◽  
Specific Primers ◽  
Potential Donor ◽  
Tissue Samples ◽  
Porcine Endogenous Retrovirus ◽  
Human Primate

Pigs are used as potential donor animals for xenotransplantation. However, porcine endogenous retrovirus (PERV), shown to infect both human and non-human primate (NHP) cells in vitro, presents a risk of transmission to humans in xenotransplantation. In this study, we analyzed PERV transmission in various organs after pig-to-NHP xenotransplantation. We utilized pig-to-NHP xenotransplant tissue samples obtained using two types of transgenic pigs from the National Institute of Animal Science (NIAS, Republic of Korea), and examined them for the existence of PERV genes in different organs via PCR and RT-PCR with specific primers. To determine PERV insertion into chromosomes, inverse PCR using PERV long terminal repeat (LTR) region-specific primers was conducted. The PERV gene was not detected in NHP organs in cardiac xenotransplantation but detected in NHP bladders in renal xenotransplantation. The insertion experiment confirmed that PERVs originate from porcine donor cells rather than integrated provirus in the NHP chromosome. We also demonstrate the presence of pig cells in the NHP bladder after renal xenotransplantation using specific-porcine mitochondrial DNA gene PCR. The PERV sequence was detected in the bladder of NHPs after renal xenotransplantation by porcine cell-microchimerism but did not integrate into the NHP chromosome.

Analysis of potential porcine endogenous retrovirus transmission to baboon in vitro and in vivo

2000 ◽  
Vol 32 (5) ◽  
pp. 1163-1164 ◽  
Author(s):  
C Templin ◽  
C Schröder ◽  
A.R Simon ◽  
G Laaff ◽  
J Köhl ◽  
...  
Keyword(s):  
Endogenous Retrovirus ◽  
Porcine Endogenous Retrovirus
Sign in / Sign up

Export Citation Format

Share Document