scholarly journals The Utilization of Pineapples Waste Enzyme for the Improvement of Hydrolysis Solubility in Aquaculture Sludge

2019 ◽  
Vol 1 (2-2) ◽  
Author(s):  
M. Fatma Syahirah ◽  
R. Nazaitulshila

High volumes of sludge discharge from the aquaculture industry have relatively high pollutant content that may lead to severe local environmental problems. Anaerobic digestion is one of the technologies for sludge treatment that might be an efficient method to reduce salty aquaculture sludge production load. However, hydrolysis solubility is becoming a limitation step during the anaerobic digestion process when the occurrence of intermediate accumulation resulted from the conversion of non-soluble biopolymers to soluble organic compounds. Thus, pretreatment is required to increase the solubilization of pollutant parameters from aquaculture sludge before it is further treated in the anaerobic treatment. Therefore, this study focuses on the production of biocatalytic enzyme from the fermentation of pre-consumer supermarket waste such as pineapple dregs to increase the solubility of aquaculture sludge. The fermentation of the pineapples waste was produced via a three-month fermentation of the mixture of molasses, pineapple dregs and water, with the ratio of 1:3:10 in a tight plastic container. Apart from that, analyses showed that the enzyme possessed lipase, amylase and protease activity. The sludge solubilization pretreatment was performed at different pH values, with treatment time for 120 hours and the solubilization was evaluated by determination of Chemical Oxygen Demand (COD) and Total Ammonia Nitrogen (TAN) solubilization; the solubilization of COD and TAN increased by 80% and 50%, respectively. This finding showed that the pineapple enzyme has the capability to solubilize organic compound, which has the potential to improve hydrolysis in further anaerobic digestion process.

1986 ◽  
Vol 18 (12) ◽  
pp. 1-14 ◽  
Author(s):  
R F. V. Novaes

This paper presents a general overview of studies conducted with microorganisms involved in the anaerobic digestion process, and also some ways of applying these studies as fundamental tools in the understanding, improvement and control of the anaerobic treatment of residuals.


2012 ◽  
Vol 66 (7) ◽  
pp. 1565-1573 ◽  
Author(s):  
A. Hierholtzer ◽  
J. C. Akunna

Sodium is a known process inhibitor in anaerobic systems and impacts on methanogens through an increase of osmotic pressure or complete dehydration of microorganisms. In this study, a combination of experimental and modelling approaches has been employed to determine and simulate sodium inhibition on the anaerobic digestion process. The ADM1, which has been successfully used in modelling anaerobic processes, has been modified to include an extra inhibition function that considers the effect of sodium on acetoclastic methanogens and the impact on biogas production and composition. A non-competitive inhibition function was added to the rate of acetate uptake for the model to take into account sodium toxicity. Experimental studies consisted of both batch and reactor tests to obtain parameters for model calibration and validation. The calibrated model was used to predict the effect of ammonia nitrogen on sodium toxicity. It was found that relatively low sodium levels can bring about significant levels of process inhibition in the presence of high levels of ammonia. On the other hand, where the concentration of ammonia is relatively low, the tolerance threshold for sodium ions increases. Hence, care must be taken in the use of sodium hydroxide for pH adjustment during anaerobic digestion of protein-rich substrates.


2013 ◽  
Vol 2 (3) ◽  
pp. 165-168 ◽  
Author(s):  
M. Orikawa ◽  
H. Kamahara ◽  
Y. Atsuta ◽  
H. Daimon

Tomato and seaweed were produced by utilizing CO2 and heat discharged from power generation using biogas in Toyogawa biomass park, Japan. The biogas was obtained by anaerobic digestion with hydrothermal treatment. The hydrothermal treatment was applied to the high concentrated sewage sludge (22 % total solids (TS) dewatered sludge). The purpose of this study is to clarify the effect of hydrothermal treatment on the qualities of high concentrated sewage sludge, by analyzing particulate organic carbon (POC) and dissolved organic carbon (DOC). The hydrothermal treatment was investigated under 10-60 min of treatment time, 180-200 °C of temperature, 10-22 %-TS of sewage sludge concentration. The results showed that the DOC in each conditions increased through hydrothermal treatment. The highest DOC obtained was 67 % of total carbon concentration, when the temperature was 180 °C, treatment time was 60 min and sewage sludge concentration was 10 %-TS. Furthermore, the viscosity of treated sewage sludge was decreased by hydrothermal treatment. In batch anaerobic digestion test, methane gas production was confirmed. In addition, this study evaluated the energy balance of this system. Thus, the results of this study indicated that the possibility of application of hydrothermal treatment to high concentrated sewage sludge for anaerobic digestion process. Keywords: anaerobic reaction, hydrothermal treatment, sewage sludge, solubilization


1986 ◽  
Vol 18 (7-8) ◽  
pp. 239-248 ◽  
Author(s):  
Sung Ryong Ha ◽  
Dwang Ho Lee ◽  
Sang Eun Lee

Laboratory scale experiments were conducted to develop a mathematical model for the anaerobic digestion of a mixture of night soil and septic tank sludge. The optimum mixing ratio by volume between night soil and septic tank sludge was found to be 7:3. Due to the high solids content in the influent waste, mixed-liquor volatile suspended solids (MLVSS) was not considered to be a proper parameter for biomass concentration, therefore, the active biomass concentration was estimated based on deoxyribonucleic acid (DNA) concentration in the reactor. The weight ratio between acidogenic bacteria and methanogenic bacteria in the mixed culture of a well-operated anaerobic digester was approximately 3:2. The proposed model indicates that the amount of volatile acid produced and the gas production rate can be expressed as a function of hydraulic residence time (HRT). The kinetic constants of the two phases of the anaerobic digestion process were determined, and a computer was used to simulate results using the proposed model for the various operating parameters, such as BOD5 and volatile acid concentrations in effluent, biomass concentrations and gas production rates. These were consistent with the experimental data.


2020 ◽  
Vol 53 (2) ◽  
pp. 16840-16845
Author(s):  
Camilo Garcia-Tenorio ◽  
Mihaela Sbarciog ◽  
Eduardo Mojica-Nava ◽  
Alain Vande Wouwer

Chemosphere ◽  
2021 ◽  
pp. 130449
Author(s):  
Yiwei Liu ◽  
Xiang Li ◽  
Shaohua Wu ◽  
Zhao Tan ◽  
Chunping Yang

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