Legionella: A Promising Supplementary Indicator of Microbial Drinking Water Quality in Municipal Engineered Water Systems

Opportunistic pathogens (OPs) are natural inhabitants and the predominant disease causative biotic agents in municipal engineered water systems (EWSs). In EWSs, OPs occur at high frequencies and concentrations, cause drinking-water-related disease outbreaks, and are a major factor threatening public health. Therefore, the prevalence of OPs in EWSs represents microbial drinking water quality. Closely or routinely monitoring the dynamics of OPs in municipal EWSs is thus critical to ensuring drinking water quality and protecting public health. Monitoring the dynamics of conventional (fecal) indicators (e.g., total coliforms, fecal coliforms, and Escherichia coli) is the customary or even exclusive means of assessing microbial drinking water quality. However, those indicators infer only fecal contamination due to treatment (e.g., disinfection within water utilities) failure and EWS infrastructure issues (e.g., water main breaks and infiltration), whereas OPs are not contaminants in drinking water. In addition, those indicators appear in EWSs at low concentrations (often absent in well-maintained EWSs) and are uncorrelated with OPs. For instance, conventional indicators decay, while OPs regrow with increasing hydraulic residence time. As a result, conventional indicators are poor indicators of OPs (the major aspect of microbial drinking water quality) in EWSs. An additional or supplementary indicator that can well infer the prevalence of OPs in EWSs is highly needed. This systematic review argues that Legionella as a dominant OP-containing genus and natural inhabitant in EWSs is a promising candidate for such a supplementary indicator. Through comprehensively comparing the behavior (i.e., occurrence, growth and regrowth, spatiotemporal variations in concentrations, resistance to disinfectant residuals, and responses to physicochemical water quality parameters) of major OPs (e.g., Legionella especially L. pneumophila, Mycobacterium, and Pseudomonas especially P. aeruginosa), this review proves that Legionella is a promising supplementary indicator for the prevalence of OPs in EWSs while other OPs lack this indication feature. Legionella as a dominant natural inhabitant in EWSs occurs frequently, has a high concentration, and correlates with more microbial and physicochemical water quality parameters than other common OPs. Legionella and OPs in EWSs share multiple key features such as high disinfectant resistance, biofilm formation, proliferation within amoebae, and significant spatiotemporal variations in concentrations. Therefore, the presence and concentration of Legionella well indicate the presence and concentrations of OPs (especially L. pneumophila) and microbial drinking water quality in EWSs. In addition, Legionella concentration indicates the efficacies of disinfectant residuals in EWSs. Furthermore, with the development of modern Legionella quantification methods (especially quantitative polymerase chain reactions), monitoring Legionella in ESWs is becoming easier, more affordable, and less labor-intensive. Those features make Legionella a proper supplementary indicator for microbial drinking water quality (especially the prevalence of OPs) in EWSs. Water authorities may use Legionella and conventional indicators in combination to more comprehensively assess microbial drinking water quality in municipal EWSs. Future work should further explore the indication role of Legionella in EWSs and propose drinking water Legionella concentration limits that indicate serious public health effects and require enhanced treatment (e.g., booster disinfection).

Spatiotemporal Variability of the Nitrous Oxide Concentrations and Fluxes From a Cascaded Dammed River

Rivers have been largely considered as the source of nitrous oxide (N2O) to the atmosphere. N2O emissions from rivers could be seriously influenced by damming and exhibit unique spatiotemporal patterns in river-reservoir systems. Multiple research studies report N2O emissions from rivers with single reservoirs, but the spatiotemporal patterns and controls of N2O emissions from cascaded river-reservoir system remain unclear. In this study, we investigated the spatiotemporal variations of N2O concentrations and fluxes along a cascade damming river (Wubu River) in Southwest China. Our results showed that N2O concentrations in the Wubu River ranged from 2.5 to 283.2 nmol L−1 with a mean of 50.7 ± 52.3 nmol L−1 and were generally supersaturated with gas fluxes ranging from 11.8 to 805.6 μmol m−2 d−1. N2O concentrations and fluxes showed a significant longitudinal variation with increasing fluxes from upstream to downstream. Meanwhile, for each river-reservoir-released water continuum, local variation of N2O concentrations was also prominent. Reservoir sections and released water sections had 2.7 (1.2–7.9) and 3.4 (1.3–12.2) times higher N2O concentrations than the corresponding upstream river reaches and acted as hotpots for N2O emission. The N2O concentrations had significant correlations with organic carbon, phosphorus, and Chl-a in surface water. Furthermore, the N2O concentrations and fluxes in reservoirs had a significant correlation with hydraulic residence time and hydraulic load, suggesting that fragmentation of hydrologic conditions was an important driver for the spatial variations of N2O concentrations in the Wubu River cascade reservoirs. Our results suggested that hydraulic residence time could predict the variation pattern of N2O fluxes in this small river basin. Seasonal variations of N2O concentrations and fluxes were the highest in autumn and lowest in winter and were mainly attributed to temperature and rainfall. N2O fluxes were much higher in the Wubu River than the average levels of China’s reservoirs and global reservoirs, acting as enhanced N2O emitter. Our study highlighted that the cascade reservoirs not only act as exciters for N2O production and emissions but also form cumulative effects and local hotpots along the longitudinal dimension, which could significantly increase the complexity of the spatiotemporal variability in riverine N2O emissions. Given the increasing construction of new river dams due to growing energy demand, more research should be done to quantify the contribution of cascaded damming to riverine N2O budgets.

Multi-wave UV-photocatalysis system (UVA+UVC+VUV/Cu-N-TiO2) for efficient inactivation of microorganisms in ballast water

Ballast Water Treatment System (BWTS) is a system designed to remove biological organisms from ballast water. However, the existing BWTSs often have problems in practical applications. In this study, a multiwave ultraviolet (UV)-modified TiO2 photocatalyst biological inactivation system (longwave UV, UVA+shortwave UV, UVC+vacuum UV, VUV/Cu-N-TiO2) for microorganism inactivation in ballast water was established. The results showed that the UVA+UVC+VUV/Cu-N-TiO2 system improved the UV light quantum yield and catalyst activity in the photocatalytic reaction and fully utilized the synergistic inactivation effect of Cu-N-TiO2 photocatalyst+ multiwave UV light (UVA, UVC, and VUV) on microorganisms. Compared with 8 other photocatalytic systems, the logarithmic algae removal rate and logarithmic sterilization rate of the UVA+UVC+VUV/Cu-N-TiO2 system increased to 1.78 log and 5.86 log, respectively. The turbidity of the seawater affected the microorganism inactivation to a certain extent. The humic acid concentration should be controlled below 2 mg L−1 for the UVA+UVC+VUV/Cu-N-TiO2 system to inactivate microalgae more effectively. The multiwave UV photocatalytic system could significantly increase the lipid peroxidation products in microbial cells, rapidly reduce superoxide dismutase (SOD) activity, and degrade a large amount of chlorophyll within a short hydraulic residence time (HRT). Severe damage to the microbial cell membrane can destroy the normal functions of cells, resulting in the death of microorganisms. In conclusion, the UVA+UVC+VUV/Cu-N-TiO2 system is a potential new ballast water treatment system.

Fluometuron Removal from Agricultural Wastewater in Porous Media Filters

In the present study, six gravity filters were constructed and evaluated for the treatment of agricultural wastewater contaminated with herbicide fluometuron. Two filter types in terms of feeding strategy (i.e., batch and continuous feeding strategies), three porous media (i.e., coarse gravel, coarse zeolite and fine zeolite) and three hydraulic residence times (i.e., 1 day, 2 days and 4 days) were evaluated to find the best design and operation parameter in fluometuron removal by adsorption on porous media. Batch experiments were also conducted and the experimental data were fitted to adsorption kinetic and isotherm models. Results showed that the experimental data fitted better to the pseudo-first order model and to the Freundlich model, and the highest fluometuron adsorption was recorded for fine zeolite. The results of filter operation indicated that the most important parameter affecting fluometuron removal is the hydraulic residence time.

Simulation and optimization of hydraulic performance of small baffled subsurface flow constructed wetland

The water body inside the constructed wetland is affected by various factors, and the flow state is relatively complicated. There will always be a certain degree of low velocity area and rapid outflow phenomenon, which makes part of the space in the wetland unable to be effectively used. Based on Computational Fluid Dynamics (CFD) technology, this paper uses Fluent's porous media model and discrete phase model to establish a hydrodynamic model of up and down baffled subsurface flow constructed wetland system. The internal flow field of the wetland is simulated, and the hydraulic performance of different baffle settings and substrate laying methods in the wetland is systematically evaluated. The results show that: up and down baffled subsurface flow constructed, with the same number of baffles, the hydraulic efficiency of the first baffle at the lower part of the substrate will be greater. Compared with the position of the baffle, the increase in the number of baffles does not significantly improve the hydraulic efficiency of the constructed wetland. The substrate layer thickness ratio has a significant effect on the two parameters of the variance of the hydraulic residence time distribution (σ2) and the flow divergence (σ02). By increasing the thickness of the middle substrate, the low flow rate phenomenon caused by the small porosity substrate area of the upper layer and the rapid outflow phenomenon of the lower substrate can be improved to a certain extent, the utilization efficiency of the middle substrate layer is improved, and the hydraulic performance is increased. The research results are of great significance for improving the utilization of wetland space and ensuring its efficient decontamination and purification function.

Utilization of Semi-Continuous Algae Culture for the Treatment of Recycled Dairy Lagoon Wash Water

Background: The utilization of animal wastes in algal culture has proven to be challenging. The utilization of “free” nutrients has drawn many researchers and industries to developing business models that call for the use of these free nutrients, which comes at a cost. Some of these costs include reduced productivity, increased contamination, lower-value target markets, and lower treatment capabilities (for wastewater treatment applications). This paper evaluates the impact of dairy lagoon effluent on productivity and wastewater treatment ability. Methods: Screened dairy lagoon wash water was fed to four three square meter outdoor open paddlewheel algal cultivation reactors. The units were operated semi-continuously for one and a half years. Seasonal productivity and nutrient uptake rates for nitrogen (N) and phosphorous (N) were measured against wastewater dilution requirements. Seasonal algal species dominance was also recorded. Wastewater was added at two levels, and the lower level was supplemented with synthetic fertilizer. Results: Seasonal N uptake rates ranged from 0.5 to 1.2 grams of N uptake per square meter per day, while P uptake ranged from 0.17 to 0.3 grams of P per square meter per day depending on season and hydraulic residence time (HRT). N removal efficiency ranged at 40 to 70% for semicontinuous operation, depending on HRT, season, and dilution of influent wastewater, which was made up from 1.5% to 13% of the daily water exchange. Conclusion: Algal reactors tended to be N limited due to the inability to add enough dairy wastewater to mitigate the high turbidity and dark color. Treatments with lower levels of added dairy wastewater tended to show higher nutrient removal. Algal culture from dairy wash water could benefit from a pretreatment step to reduce turbidity and color, promoting algal growth and productivity.

Anaerobic Degradation of Diesel Fuel Contaminated Wastewater in a Fluidized Bed Rector

This thesis studies the performance of the Anaerobic Fluidized Bed Reactor (AFBR) in treating diesel fuel contaminated wastewaters. The AFBR is a semi-cyclindrical fluidized bed, with a capacity of 300 L and height of 2.90 m with sampling ports along the column length. Granular activated carbon (12-20 mesh) was used as the medium to immobilize biomass. Diesel fuel was the sole source of carbon for microorganisms. The system's COD removal capability and diesel fuel removal efficiency were measured at 100 mg/L, 200 mg/L, and 300 mg/L influent diesel fuel in the reactor. Hydraulic Residence Time (HRT) varied for each set of experiments from 96 to 6 hours. The system achieved diesel removal efficiency of more than 84.1% for 300 mg/L influent diesel concentration for the maximum flowrate treated (1200 L/d) at a minimum HRT of 6 hours. This investigation confirms that anaerobic degradation of diesel contaminated water can be carried out very effectively in the AFBR.

Anaerobic Degradation of Diesel Fuel Contaminated Wastewater in a Fluidized Bed Rector

This thesis studies the performance of the Anaerobic Fluidized Bed Reactor (AFBR) in treating diesel fuel contaminated wastewaters. The AFBR is a semi-cyclindrical fluidized bed, with a capacity of 300 L and height of 2.90 m with sampling ports along the column length. Granular activated carbon (12-20 mesh) was used as the medium to immobilize biomass. Diesel fuel was the sole source of carbon for microorganisms. The system's COD removal capability and diesel fuel removal efficiency were measured at 100 mg/L, 200 mg/L, and 300 mg/L influent diesel fuel in the reactor. Hydraulic Residence Time (HRT) varied for each set of experiments from 96 to 6 hours. The system achieved diesel removal efficiency of more than 84.1% for 300 mg/L influent diesel concentration for the maximum flowrate treated (1200 L/d) at a minimum HRT of 6 hours. This investigation confirms that anaerobic degradation of diesel contaminated water can be carried out very effectively in the AFBR.