scholarly journals High Quality of Bacterial DNA Extraction from Corbicula fluminea Tissue in Kelantan

2020 ◽  
Vol 16 (2) ◽  
pp. 161-165
Author(s):  
Koh Han Dee ◽  
Suganthi Appalasamy ◽  
Siti Nor Aini Md Nasir ◽  
Faizuan Abdullah ◽  
Maryana Mohamad Nor ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Cetyltrimethylammonium Bromide ◽  
Corbicula Fluminea ◽  
High Quality ◽  
Bacterial Dna ◽  
Tissue Surface ◽  
High Integrity ◽  
Effectiveness And Efficiency ◽  
Bacterial Dna Extraction

Corbicula fluminea served as a popular traditional food in Kelantan, Malaysia. This clam is highly consumed by the local people from the last decade. However, there is lack of the study that obtain high quality of bacterial metagenome deoxyribonucleic acid (DNA) extraction from C. fluminea. Improper DNA extraction protocol always fails to extract a high integrity of bacterial DNA bands. This study compares the effectiveness and efficiency of conventional cetyltrimethylammonium bromide (CTAB) protocol, a commercial kit and modified CTAB protocol for bacterial DNA extraction from the soft tissue surface of raw C. fluminea. The instruments used to examine the quality of the extracted bacterial DNA were DeNovix DS-11 spectrophotometer, gel electrophoresis machine and UV transilluminator. The results showed that the bacterial DNA extracted from modified CTAB protocol the highest purity and integrity with the A₂₆₀/A₂₈₀ ratio of 1.92 ± 0.01 as well as the DNA band with minimum smear. This conclude that modified CTAB protocol is the best among the three extraction protocols for the bacterial extraction from the C. fluminea.

Quality Improvement of the DNA extracted by boiling method in Gram negative bacteria

2017 ◽  
Vol 6 (04) ◽  
pp. 5347 ◽  
Author(s):  
Omar B. Ahmed* ◽  
Anas S. Dablool
Keyword(s):  
Dna Extraction ◽  
Control Method ◽  
Extraction Procedure ◽  
Cost Effective ◽  
High Yield ◽  
Gram Negative Bacteria ◽  
Bacterial Dna ◽  
Gram Negative ◽  
E Coli

Several methods of Deoxyribonucleic acid (DNA) extraction have been applied to extract bacterial DNA. The amount and the quality of the DNA obtained for each one of those methods are variable. The study aimed to evaluate bacterial DNA extraction using conventional boiling method followed by alcohol precipitation. DNA extraction from Gram negative bacilli was extracted and precipitated using boiling method with further precipitation by ethanol. The extraction procedure performed using the boiling method resulted in high DNA yields for both E. coli and K. pneumoniae bacteria in (199.7 and 285.7μg/ml, respectively) which was close to control method (229.3 and 440.3μg/ml). It was concluded that after alcohol precipitation boiling procedure was easy, cost-effective, and applicable for high-yield quality of DNA in Gram-negative bacteria.

scholarly journals Choice of bacterial DNA extraction method from fecal material influences community structure as evaluated by metagenomic analysis

Microbiome ◽  
2014 ◽  
Vol 2 (1) ◽  
pp. 19 ◽  
Author(s):  
Agata Wesolowska-Andersen ◽  
Martin Bahl ◽  
Vera Carvalho ◽  
Karsten Kristiansen ◽  
Thomas Sicheritz-Pontén ◽  
...  
Keyword(s):  
Community Structure ◽  
Dna Extraction ◽  
Extraction Method ◽  
Metagenomic Analysis ◽  
Fecal Material ◽  
Bacterial Dna ◽  
Dna Extraction Method ◽  
Bacterial Dna Extraction

scholarly journals Necessity of purification during bacterial DNA extraction with environmental soils

2017 ◽  
Vol 32 ◽  
pp. e2017013 ◽  
Author(s):  
Hyun Jeong Lim ◽  
Jung-Hyun Choi ◽  
Ahjeong Son
Keyword(s):  
Dna Extraction ◽  
Bacterial Dna ◽  
Bacterial Dna Extraction

scholarly journals A New, Rapid, Efficient and Non-Toxic Method for Bacterial DNA Extraction

2019 ◽  
Vol 116 (3) ◽  
pp. 360a
Author(s):  
Semire Uzun Gocmen ◽  
Ahmet Aslan ◽  
Muhyittin Temiz
Keyword(s):  
Dna Extraction ◽  
Bacterial Dna ◽  
Bacterial Dna Extraction

Development of a bacterial DNA extraction modular chip using a magnetic particle and portable vibration motor

2020 ◽  
Vol 12 (9) ◽  
pp. 1197-1202
Author(s):  
Sun Young Lim ◽  
Tae Jae Lee ◽  
Seol Yi Shin ◽  
Nam Ho Bae ◽  
Seok Jae Lee ◽  
...  
Keyword(s):  
Power System ◽  
Electric Power ◽  
Dna Extraction ◽  
Magnetic Particles ◽  
Magnetic Particle ◽  
Electric Power System ◽  
Bacterial Dna ◽  
System P ◽  
3D Printed ◽  
Bacterial Dna Extraction

The bacterial DNA was simply purified by magnetic particles with a portable vibration motor. To effectively extract DNA in the field, the 3D-printed device was employed with low electric power system.

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