scholarly journals An assessment of differences in the ruminal degradability and intestinal digestibility of crude protein in brewer’s grains and maize draff

Author(s):  
Vladimír Majer ◽  
Pavel Veselý

The submitted thesis aims to assess the differences between the ruminal degradability and intestinal digestibility of crude protein contained in brewer’s grains (BG) and maize draff (AMG). The effectiveness of ruminal degradability was tested using the “in sacco” method on 3 dry Holstain cows fitted with rumen cannulas. The dynamics of ruminal degradability of crude protein (CP) was detected after 0, 4, 8, 16, and 24 hours of samples incubation in the rumen. The intestinal digestibility of crude protein undegradable in the rumen was determined using the “mobile bag” method on 3 dry Holstain cows fitted with duodenal cannulas. The crude protein degradability of BG was detected in the above-mentioned hours (%): 4.06; 18.16; 32.40; 38.56, and 50.70; crude protein degradability of AMG: 42.04; 63.56; 84.47; 85.16, and 87.19. The effectiveness of rumen degradability of BG crude protein at the rate of passage of rumen content 6 % per hour was calculated at 35.33 % and that of AMG, at 76.29 %. Intestinal digestibility of BG crude protein and dry matter at the rate of passage of intestinal content 6 % per hour was calculated at 79.41 % and 22.84 %, respectively, and that of AMG, at 57.01 % and 11.33 %, respectively. The differences between the indicators of both feedstuffs were significant (P < 0.05). The results show that BG are mostly a source of crude protein with higher intestinal digestibility than AMG.

2019 ◽  
Vol 40 (6Supl3) ◽  
pp. 3605
Author(s):  
Ernestina dos Ribeiro Santos Neta ◽  
Luis Rennan Sampaio Oliveira ◽  
Rafael Mezzomo ◽  
Daiany Íris Gomes ◽  
Janaina Barros Luz ◽  
...  

This study evaluated the chemical composition and ruminal degradability of dry matter (DM), neutral detergent fiber corrected for ash and protein (NDFap) and crude protein (CP) in byproducts of African oil palm (palm cake, kernel or fiber), macaúba (pulp cake and kernel cake), acai (acai fruit), babassu (kernel cake) and pineapple (peel, crown and bagasse silage). Nineteen rumen-fistulated sheep were kept in individual stalls, receiving a daily diet composed of elephant grass silage and corn and soybean concentrate. After preparation in nylon bags, the byproduct samples were incubated for 0, 3, 6, 12, 16, 18, 24, 48, 72, 96, 120 and 144 hours, with three replicates of each ingredient per incubation time. The divergence between the protein nutritional value and energy nutritional value, based on discriminatory variables between groups, was estimated by cluster analysis. The effective degradability of DM, NDFap and CP for the different byproducts was, respectively, 35.9, 26.9 and 59.0% for palm cake; 48.3, 34.3 and 76.4% for palm kernel; 21.1, 6.6 and 50.3% for palm fiber; 34.3, 15.0 and 52.8% for macaúba pulp cake; 58.1; 63.0 and 51.6% for macaúba kernel cake; 49.7, 49.6 and 41.8% for babassu cake; 53.4, 40.5 and 79.8% for pineapple bagasse silage; and 21.3, 17.0 and 38.9% for acai fruit. Based on their NDFap and CP characteristics, the feeds were clustered in up to four different groups.


2007 ◽  
Vol 2007 ◽  
pp. 168-168
Author(s):  
P. Shawrang ◽  
A.A. Sadeghi

Safflower meal proteins are extensively degraded in the rumen. Attempts to decrease the degradability of feedstuffs proteins have involved treatment with heat, formaldehyde, tannic acid, acetic acid, xylose, and microwave (Sadeghi et al., 2006). To our knowledge, no information is available concerning effects of gamma irradiation on ruminal protein degradation and type of safflower meal proteins that leave the rumen undegraded. The objectives of this study were to investigate effects of gamma irradiation on ruminal degradability and intestinal digestibility of safflower meal crude protein, and to monitor fate of true proteins of safflower meal in the rumen by using SDS-PAGE methodology.


2017 ◽  
Vol 9 (7) ◽  
pp. 164 ◽  
Author(s):  
Renato Tonhá Alves Júnior ◽  
Evaristo Jorge Oliveira de Souza ◽  
Airon Aparecido Silva de Melo ◽  
Dulciene Karla De Andrade Silva ◽  
Thaysa Rodrigues Torres ◽  
...  

Four concentrations (0, 200, 400, 600 and 800 mg extract per ml of water) of mesquite extract were used as phytogenic additive to verify the potential to increase the nutritional value of the feed, ruminal parameters (primarily propionate production) and nitrogen use efficiency, microbial protein synthesis and quantify the reduction of ciliated protozoa and characterize the ingestive behavior of sheep. Ten adult male sheep were subjected to a 5 × 5 double Latin square design. Prior to feeding, the animals received the mesquite extract. Nutrient intake was estimated from the difference of the amount of feed provided and the total surplus. Rumen content samples were collected to evaluate the profile of short-chain fatty acids, ammonia nitrogen, pH, ciliated protozoa, turnover rate and disappearance rate. To estimate the microbial protein synthesis, the technique of purine derivatives was used. The mesquite extract quadratically increased (P < 0.05) the digestibility of dry matter, organic matter, crude protein and total digestible nutrients, as well as increased propionate production, acetate:propionate ratio and microbial protein synthesis. The numbers of ciliate protozoa in the rumen decreased as a result of mesquite extract inclusion in the diet. The use of mesquite pod extract at a concentration of 488 mg/mL is recommended to improve digestibility of dry matter, organic matter, crude protein and total digestible nutrients, and to optimize microbial protein synthesis and increase propionic acid production.


2002 ◽  
Vol 74 (2) ◽  
pp. 337-345 ◽  
Author(s):  
J. Faría-Mármol ◽  
J. González ◽  
C.A. Rodríguez ◽  
M. R. Alvir

AbstractThe ruminal degradation of dry matter (DM) and crude protein (CP) and the intestinal availability of CP of four lucerne samples were measured on two diets with lucerne hay to concentrate ratios of 2: 1 (diet F) and 1: 2 (diet C). Two samples of fresh lucerne (third cut) harvested after 2 (FL1) or 8 (FL2) weeks from the previous cut were used together with a sample of lucerne hay (LH) and another of dehydrated lucerne (DL). Rumen degradability, measured by the nylon bag technique, and rumen outflow rates were determined on three rumen cannulated wethers. Intestinal digestibility was determined by the mobile bag technique on three duodenal fistulated wethers. For CP, significantly lower values were observed with diet C than with diet F for the potentially degradable insoluble fraction (0·334 v. 0·397) and its degradation rate (0·093 v. 0·134 per h). As a consequence, the effective degradability was also lower with diet C (0·746 v. 0·821; P = 0·059). Effective degradability of DM was also apparently lower with diet C (0·596 v. 0·634). With both diets, the intestinal digestibility decreased in all the samples with increase of ruminal incubation time according to a simple exponential equation. The undegraded CP digested in the gut (Di) and therefore the effective intestinal digestibility (EID) were derived from this exponential function according to the rumen outflow of undegraded CP. Mean values of Di (expressed as proportion of food CP content) were respectively 0·091 and 0·142 for F and C diets and 0·084, 0·115, 0·116, and 0·152 for FL1, FL2, LH and DL samples. Lower rumen degradability was partially compensated for by higher Di values resulting in a close correlation between both parameters (r = –0·965; P 0·001). The change of the digestion site associated with the reduction of the effective degradability of CP produced also an increase in the undigested CP as a proportion of food CP. So, these values are respectively 0·087 and 0·112 for F and C diets and 0·053, 0·109, 0·096, and 0·141 for FL1, FL2, LH, and DL samples. No difference in EID between F and C diets was observed (0·529 v. 0·563). For samples, the only effect (P 0·05) was recorded between FL1 (0·618) and the other samples (0·509, 0·544 and 0·512 for FL2, LH, and DL, respectively).


1995 ◽  
Vol 75 (4) ◽  
pp. 559-565 ◽  
Author(s):  
K. A. Beauchemin ◽  
D. R. C. Bailey ◽  
T. A. McAllister ◽  
K.-J. Cheng

A study was conducted to determine the effects of lignosulfonate and heat treatment of canola meal on protein degradability, and to evaluate whether feeding treated canola meal to nursing beef calves enhances preweaning growth. Four supplements were formulated to contain 31% crude protein (CP) using either: (1) untreated canola meal (UTC), (2) heat-treated canola meal (HTC; 100 °C for 1 h), (3) lignosulfonate-treated canola meal (LTC; 5% calcium lignosulfonate and 100 °C for 1 h), or (4) corn distillers' dried grains (DDG). Degradability of canola meal CP was reduced significantly from 55.1% (measured in sacco) to 43.2% by heat, and to 26.7% by lignosulfonate treatment. One hundred and three nursing crossbred calves were given access to either no supplement (control) or one of the four supplements. Weight gain (kg d−1) increased (P < 0.05) from 0.90 for the control to 1.05 for calves fed DDG, and further increased (P < 0.05) to 1.16, 1.16 and 1.18 for calves fed UTC, HTC, and LTC, respectively. Consequently, calves fed canola meal gained 30.8 kg more (P < 0.05) than those fed control, and 12.8 kg more (P < 0.05) than those fed DDG. The performance of suckling calves during the grazing season and body condition of their dams were enhanced by offering canola-based creep feeds. Although degradability of canola meal CP was substantially lowered by lignosulfonate treatment, calf performance was not further enhanced by feeding treated canola meal. Key words: Beef cattle, creep feeding, canola meal, lignosulfonate, heat-treatment, distillers' dried grains, protein degradability


2000 ◽  
Vol 49 (5) ◽  
pp. 391-398 ◽  
Author(s):  
Madiha Haj-Ayed ◽  
Javier González ◽  
Rafael Caballero ◽  
María Remedios Alvir

2020 ◽  
pp. 2391-2400
Author(s):  
Valter Harry Bumbieris Junior ◽  
Robson Marcelo Rossi ◽  
Egon Henrique Horst ◽  
Murilo Dolfini Paranzini ◽  
Vinicius André de Pietro Guimarães ◽  
...  

The objective of this study was to evaluate the ruminal degradability of dry matter and crude protein of high moisture triticale silage ensiled with different chemical and biological additives. Urea, sodium benzoate and an enzyme-bacterial inoculant were used as treatments. Four samples from each treatment were incubated in rumen on four sheep. Effective degradability was estimated for ruminal passage rate of 2%, 5% and 8% hour-1. Bayesian procedures were used to estimate potential degradation parameters in situ. The high moisture triticale silage with urea showed highest value for the soluble fraction (70.46%) and the best effective dry matter degradability, with a passing rate of 2% h-1 (90.63%), of control silage at other rates of passage. In relation to control silage, the addition of sodium benzoate and enzyme-bacterial inoculant decreased the effective degradability of dry matter, regardless of rate passage evaluated. Due to high solubility of urea, the silage added with this additive had the highest soluble fraction of crude protein (76.42%). The addition of enzyme-bacterial inoculant accelerated the ruminal passage rate of dry matter and protein to 0.26 and 0.20% h-1, respectively, providing less potential degradability of both in relation to other silages. As enzyme-bacterial inoculation reduces rumen degradability of crude protein, it tends to increase the availability of amino acids for intestinal absorption. The addition of urea to high moisture triticale silage may be recommended for sheep feeding at a low level of consumption, as it improves the effective dry matter degradability.


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