Blue LED light photobiomodulation in cultured human fibroblasts and keratinocytes cell line

In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts and keratinocytes. Different light doses were used to treat the cells, evidencing inhibitory and stimulatory effects. Electrophysiology was used to investigate the effects on membrane currents, while Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation. In conclusion, we observed that the blue LED light can be used to modulate the activity of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

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Isoleucine transport by cultured human fibroblasts. II. Selection of a cell line with reduced isoleucine uptake

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/0005-2736(74)90138-2 ◽

1974 ◽

Vol 367 (1) ◽

pp. 81-87 ◽

Cited By ~ 5

Author(s):

Richard E Hillman ◽

Elaine F Otto

Keyword(s):

Cell Line ◽

Human Fibroblasts ◽

Cultured Human Fibroblasts ◽

A Cell ◽

Selection Of

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Experimental Study on Blue Light Interaction with Human Keloid-Derived Fibroblasts

Biomedicines ◽

10.3390/biomedicines8120573 ◽

2020 ◽

Vol 8 (12) ◽

pp. 573 ◽

Cited By ~ 1

Author(s):

Giada Magni ◽

Martina Banchelli ◽

Federica Cherchi ◽

Elisabetta Coppi ◽

Marco Fraccalvieri ◽

...

Keyword(s):

Cell Metabolism ◽

Human Fibroblasts ◽

Specific Staining ◽

Invasive Approach ◽

Blue Led ◽

Led Light ◽

Electrophysiological Recordings ◽

Outward Currents ◽

Voltage Dependent ◽

Physical Treatments

Keloids are an exuberant response to wound healing, characterized by an exaggerated synthesis of collagen, probably due to the increase of fibroblasts activity and to the reduction of their apoptosis rate: currently no standard treatments or pharmacological therapies are able to prevent keloid recurrence. To reach this goal, in recent years some physical treatments have been proposed, and among them the PhotoBioModulation therapy (PBM). This work analyses the effects of a blue LED light irradiation (410–430 nm, 0.69 W/cm2 power density) on human fibroblasts, isolated from both keloids and perilesional tissues. Different light doses (3.43–6.87–13.7–20.6–30.9 and 41.2 J/cm2) were tested. Biochemical assays and specific staining were used to assess cell metabolism, proliferation and viability. Micro-Raman spectroscopy was used to explore direct effects of the blue LED light on the Cytochrome C (Cyt C) oxidase. We also investigated the effects of the irradiation on ionic membrane currents by patch-clamp recordings. Our results showed that the blue LED light can modulate cell metabolism and proliferation, with a dose-dependent behavior and that these effects persist at least till 48 h after treatment. Furthermore, we demonstrated that the highest fluence value can reduce cell viability 24 h after irradiation in keloid-derived fibroblasts, while the same effect is observed 48 h after treatment in perilesional fibroblasts. Electrophysiological recordings showed that the medium dose (20.6 J/cm2) of blue LED light induces an enhancement of voltage-dependent outward currents elicited by a depolarizing ramp protocol. Overall, these data demonstrate the potentials that PBM shows as an innovative and minimally-invasive approach in the management of hypertrophic scars and keloids, in association with current treatments.

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Photobiomodulation of Human Fibroblasts and Keratinocytes with Blue Light: Implications in Wound Healing

Biomedicines ◽

10.3390/biomedicines9010041 ◽

2021 ◽

Vol 9 (1) ◽

pp. 41

Author(s):

Francesca Rossi ◽

Giada Magni ◽

Francesca Tatini ◽

Martina Banchelli ◽

Federica Cherchi ◽

...

Keyword(s):

Wound Healing ◽

Blue Light ◽

Chronic Wounds ◽

Human Fibroblasts ◽

Healing Process ◽

Research Area ◽

Wound Management ◽

Blue Led ◽

Led Light ◽

New Research

In recent years, photobiomodulation (PBM) has been recognized as a physical therapy in wound management. Despite several published research papers, the mechanism underlying photobiomodulation is still not completely understood. The investigation about application of blue light to improve wound healing is a relatively new research area. Tests in selected patients evidenced a stimulation of the healing process in superficial and chronic wounds treated with a blue LED light emitting at 420 nm; a study in animal model pointed out a faster healing process in superficial wound, with an important role of fibroblasts and myofibroblasts. Here, we present a study aiming at evidencing the effects of blue light on the proliferation and metabolism in fibroblasts from healthy skin and keratinocytes. Different light doses (3.43, 6.87, 13.7, 20.6, 30.9 and 41.2 J/cm2) were used to treat the cells, evidencing inhibitory and stimulatory effects following a biphasic dose behavior. Electrophysiology was used to investigate the effects on membrane currents: healthy fibroblasts and keratinocytes showed no significant differences between treated and not treated cells. Raman spectroscopy revealed the mitochondrial Cytochrome C (Cyt C) oxidase dependence on blue light irradiation: a significant decrease in peak intensity of healthy fibroblast was evidenced, while it is less pronounced in keratinocytes. In conclusion, we observed that the blue LED light can be used to modulate metabolism and proliferation of human fibroblasts, and the effects in wound healing are particularly evident when studying the fibroblasts and keratinocytes co-cultures.

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Experience in application of red and blue LED light with infra+red component in the ENT disease treatment in children

SOVREMENNAYA PEDIATRIYA ◽

10.15574/sp.2018.89.73 ◽

2018 ◽

pp. 73-79

Author(s):

A.L. Kosakovskyi ◽

◽

S.O. Gulyar ◽

I.A. Kosakivska ◽

N.P. Grushetska ◽

...

Keyword(s):

Disease Treatment ◽

Blue Led ◽

Led Light ◽

Infra Red

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In vitro Three Dimensional Scaffold-free Construct of Human Adipose-derived Stem Cells in Coculture with Endothelial Cells and Fibroblasts

Revista de Chimie ◽

10.37358/rc.17.6.5670 ◽

2017 ◽

Vol 68 (6) ◽

pp. 1341-1344

Author(s):

Grigore Berea ◽

Gheorghe Gh. Balan ◽

Vasile Sandru ◽

Paul Dan Sirbu

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Endothelial Cells ◽

Single Cell ◽

Cell Line ◽

Bone Repair ◽

Umbilical Vein ◽

Human Fibroblasts ◽

Three Dimensional ◽

Adipose Derived Stem Cells

Complex interactions between stem cells, vascular cells and fibroblasts represent the substrate of building microenvironment-embedded 3D structures that can be grafted or added to bone substitute scaffolds in tissue engineering or clinical bone repair. Human Adipose-derived Stem Cells (hASCs), human umbilical vein endothelial cells (HUVECs) and normal dermal human fibroblasts (NDHF) can be mixed together in three dimensional scaffold free constructs and their behaviour will emphasize their potential use as seeding points in bone tissue engineering. Various combinations of the aforementioned cell lines were compared to single cell line culture in terms of size, viability and cell proliferation. At 5 weeks, viability dropped for single cell line spheroids while addition of NDHF to hASC maintained the viability at the same level at 5 weeks Fibroblasts addition to the 3D construct of stem cells and endothelial cells improves viability and reduces proliferation as a marker of cell differentiation toward osteogenic line.

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Photocontrolled iodine-mediated reversible-deactivation radical polymerization with a semifluorinated alternating copolymer as the macroinitiator

Polymer Chemistry ◽

10.1039/d0py01357a ◽

2020 ◽

Vol 11 (47) ◽

pp. 7497-7505

Author(s):

Jiannan Cheng ◽

Kai Tu ◽

Enjie He ◽

Jinying Wang ◽

Lifen Zhang ◽

...

Keyword(s):

Block Copolymers ◽

Radical Polymerization ◽

Room Temperature ◽

Blue Led ◽

Led Light ◽

Alternating Copolymers ◽

Alternating Copolymer ◽

Reversible Deactivation ◽

Novel Strategy ◽

Reversible Deactivation Radical Polymerization

A novel strategy for preparing block copolymers with semifluorinated alternating copolymers as macroinitiators was established by photocontrolled iodine-mediated RDRP under irradiation with blue LED light at room temperature.

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