Accuracy of Three Dry-Chemistry Methods for Lipid Profiling and Risk Factor Classification

2003 ◽  
Vol 13 (3) ◽  
pp. 358-368 ◽  
Author(s):  
Daniela A. Rubin ◽  
Robert G. McMurray ◽  
Joanne S. Harrell ◽  
Barbara W. Carlson ◽  
Shrikant Bangdiwala
Keyword(s):  
Risk Factor ◽  
High Density Lipoprotein ◽  
Total Cholesterol ◽  
Venous Blood ◽  
Density Lipoprotein ◽  
Reference Method ◽  
Risk Classification ◽  
Lipid Profiling ◽  
Chi Square ◽  
Portable Analyzer

The purpose of this project was to determine the accuracy in lipids measurement and risk factor classification using Reflotron, Cholestech, and Ektachem DT-60 dry-chemistry analyzers. Plasma and capillary venous blood from fasting subjects (n = 47) were analyzed for total cholesterol (TC), high density lipoprotein (HDL-C), and triglycerides (TG) using these analyzers and a CDC certified laboratory. Accuracy was evaluated by comparing the results of each portable analyzer against the CDC reference method. One-way ANOVAs were performed for TC, HDL-C, and TG between all portable analyzers and the reference method. Chi-square was used for risk classification (2001 NIH Guidelines). Compared to the reference method, the Ektachem and Reflotron provided significantly lower values for TC (p < .05). In addition, the Cholestech and Ektachem values for HDL-C were higher than the CDC (p < .05). The Reflotron and Cholestech provided higher values of TG than the CDC (p < .05). Chi-squares analyses for risk classification were not significant (p > .45) between analyzers. According to these results, the Ektachem and Cholestech analyzers met the current NCEP III guidelines for accuracy in measurement of TC, while only Ektachem met guidelines for TG. All 3 analyzers provided a good overall risk classification; however, values of HDL-C should be only used for screening purposes.

scholarly journals Hubungan antara Rasio Kadar Kolesterol Total terhadap High Density Lipoprotein (HDL) dengan Kejadian Sindrom Koroner Akut di RSUP Prof. Dr. R. D. Kandou Manado

e-CliniC ◽  
2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Mercilia F. Wenas ◽  
Edmond L. Jim ◽  
Agnes L. Panda
Keyword(s):  
Acute Coronary Syndrome ◽  
Heart Disease ◽  
High Density Lipoprotein ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Density Lipoprotein ◽  
High Density ◽  
Chi Square ◽  
Coronary Syndrome ◽  
Chi Square Test

Abstract: From year to year, heart disease cases show an increase as the leading cause of death in the world. Acute coronary syndrome (ACS) is a set of manifestations or symptoms caused by a disturbance in the coronary artery. Blood cholesterol level is one of the major risk factors underlying ACS. High total cholesterol and low high density lipoprotein (HDL) levels will increase the ratio of both and are directly related to increased coronary heart disease. The ratio can be obtained by dividing total cholesterol level with HDL level. This study was aimed to obtain the correlation between ratio of total cholesterol to (HDL) with the incidence of ACS at Prof. Dr. R. D. Kandou Hospital Manado. This was a descriptive analytical study with a retrospective cross sectional design using medical record data. Data were analyzed by using the chi-square test. There were 117 patients obtained by using total sampling technique. The chi-square test showed a P value of 0.026 (P <0,05). Conclusion: There was a significant correlation between the ratio of total cholesterol to HDL and the incidence of ACS.Keywords: acute coronary syndrome, ratio of total cholesterol to HDL. Abstrak: Kasus penyakit jantung dari tahun ke tahun menunjukkan adanya peningkatan sebagai penyebab kematian tertinggi di dunia. Sindrom koroner akut (SKA) merupakan sekumpulan manifestasi atau gejala akibat gangguan pada arteri koronaria. Kadar kolesterol darah merupakan faktor risiko utama proses aterosklerosis yang mendasari terjadinya sindrom koroner akut. Kadar kolesterol total yang tinggi dan HDL yang rendah akan meningkatkan rasio dari keduanya dan berhubungan langsung dengan peningkatan risiko penyakit jantung koroner. Rasio dapat diperoleh dengan cara membagi kadar kolesterol total dengan HDL. Penelitian ini bertujuan untuk mengetahui hubungan antara rasio kadar kolestrol total terhadap high density lipoprotein (HDL) dengan kejadian SKA di RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian ialah deskriptif analitik dengan desain potong lintang dan retrospektif menggunakan data rekam medis pasien. Data dianalisis dengan uji chi-square. Jumlah sampel penelitian sebanyak 117 pasien diperoleh dengan teknik total sampling. Hasil uji chi-square menunjukkan nilai P=0,026 (P <0,05). Simpulan: Terdapat hubungan bermakna antara rasio kadar kolesterol total terhadap HDL dengan kejadian SKA.Kata kunci: rasio kadar kolesterol total terhadap HDL, sindrom koroner akut

Triglyceride to high density lipoprotein cholesterol ratio, total cholesterol to high density lipoprotein cholesterol ratio and low ankle brachial index in an elderly population

VASA ◽  
2014 ◽  
Vol 43 (3) ◽  
pp. 189-197 ◽  
Author(s):  
Yiqiang Zhan ◽  
Jinming Yu ◽  
Rongjing Ding ◽  
Yihong Sun ◽  
Dayi Hu
Keyword(s):  
High Density Lipoprotein ◽  
Total Cholesterol ◽  
High Density Lipoprotein Cholesterol ◽  
Ankle Brachial Index ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Lipid Lowering ◽  
Cholesterol Ratio ◽  
Potential Biomarker

Background: The associations of triglyceride (TG) to high-density lipoprotein cholesterol ratio (HDL‑C) and total cholesterol (TC) to HDL‑C ratio and low ankle brachial index (ABI) were seldom investigated. Patients and methods: A population based cross-sectional survey was conducted and 2982 participants 60 years and over were recruited. TG, TC, HDL‑C, and low-density lipoprotein cholesterol (LDL-C) were assessed in all participants. Low ABI was defined as ABI ≤ 0.9 in either leg. Multiple logistic regression models were applied to study the association between TG/HDL‑C ratio, TC/HDL‑C ratio and low ABI. Results: The TG/HDL‑C ratios for those with ABI > 0.9 and ABI ≤ 0.9 were 1.28 ± 1.20 and 1.48 ± 1.13 (P < 0.0001), while the TC/HDL‑C ratios were 3.96 ± 1.09 and 4.32 ± 1.15 (P < 0.0001), respectively. After adjusting for age, gender, body mass index, obesity, current drinking, physical activity, hypertension, diabetes, lipid-lowering drugs, and cardiovascular disease history, the odds ratios (ORs) with 95 % confidence intervals (CIs) of low ABI for TG/HDL‑C ratio and TC/HDL‑C ratio were 1.10 (0.96, 1.26) and 1.34 (1.14, 1.59) in non-smokers. When TC was further adjusted, the ORs (95 % CIs) were 1.40 (0.79, 2.52) and 1.53 (1.21, 1.93) for TG/HDL‑C ratio and TC/HDL‑C ratio, respectively. Non-linear relationships were detected between TG/HDL‑C ratio and TC/HDL‑C ratio and low ABI in both smokers and non-smokers. Conclusions: TC/HDL‑C ratio was significantly associated with low ABI in non-smokers and the association was independent of TC, TG, HDL‑C, and LDL-C. TC/HDL‑C might be considered as a potential biomarker for early peripheral arterial disease screening.

scholarly journals Reference intervals for serum lipids and prevalence of dyslipidaemia in 6–12-year-old children: The Health Oriented Pedagogical Project (HOPP)

2018 ◽  
Vol 46 (21_suppl) ◽  
pp. 21-27 ◽  
Author(s):  
Martin Frank Strand ◽  
Per Morten Fredriksen ◽  
Ole Petter Hjelle ◽  
Morten Lindberg
Keyword(s):  
Total Cholesterol ◽  
Serum Lipids ◽  
Venous Blood ◽  
Elevated Serum ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Reference Intervals ◽  
Age And Gender ◽  
Norwegian Population ◽  
And Gender

Aims: Elevated serum lipid concentrations in childhood are thought to be risk factors for the development of cardiovascular disease later in life. The present study aims to provide age- and gender-related reference intervals for total cholesterol (TC), high-density lipoprotein (HDL) cholesterol, and non-HDL cholesterol in healthy school children. We also investigated the prevalence of dyslipidaemia using the published criteria for these biomarkers. Methods: Venous blood and anthropometric data were collected from 1340 children in the HOPP study, aged between 6 and 12 years. Age- and gender-related reference intervals (2.5th and 97.5th percentiles) were established according to the IFCC recommendations, using the software RefVal 4.10. Results: Gender differences were observed for total cholesterol and non-HDL cholesterol, but not for HDL cholesterol. Age differences were observed for total cholesterol. The reference intervals were in the range of 3.1–5.9 mmol/L for total cholesterol, 1.0–2.4 mmol/L for HDL cholesterol and 1.4–4.2 mmol/L for non-HDL cholesterol. Dyslipidaemia prevalence was as follows: increased TC 9.6%, decreased HDL 1.6%, and increased non-HDL 5.6%. Conclusions: Age- and gender-related reference intervals in a Norwegian population are similar to those reported in other countries. The prevalence of dyslipidaemia among Norwegian children is significant, emphasising the importance of appropriate reference intervals in clinical practice.

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