scholarly journals Enhancing Drug Accumulation in Saccharomyces cerevisiae by Repression of Pleiotropic Drug Resistance Genes with Chimeric Transcription Repressors

2008 ◽  
Vol 74 (2) ◽  
pp. 423-431 ◽  
Author(s):  
Alexander Stepanov ◽  
Karin C. Nitiss ◽  
Geoffrey Neale ◽  
John L. Nitiss
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Resistance Genes ◽  
Drug Accumulation ◽  
Pleiotropic Drug Resistance ◽  
Transcription Repressors

Upregulated pleiotropic drug resistance genes in Saccharomyces cerevisiae yrr1–52

The Nucleus ◽  
2015 ◽  
Vol 58 (3) ◽  
pp. 231-234
Author(s):  
Naohiko Kodo ◽  
Shoei Sakata ◽  
Toshiro Matsuda
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Resistance Genes ◽  
Pleiotropic Drug Resistance

Pleiotropic drug-resistance attenuated genomic library improves elucidation of drug mechanisms

2015 ◽  
Vol 11 (11) ◽  
pp. 3129-3136 ◽  
Author(s):  
Namal V. C. Coorey ◽  
James H. Matthews ◽  
David S. Bellows ◽  
Paul H. Atkinson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Mechanism Of Action ◽  
Gene Deletion ◽  
Genomic Library ◽  
Deletion Mutants ◽  
Pleiotropic Drug Resistance ◽  
Genome Wide

Identifying Saccharomyces cerevisiae genome-wide gene deletion mutants that confer hypersensitivity to a xenobiotic aids the elucidation of its mechanism of action (MoA).

Heterologous carotenoid production in Saccharomyces cerevisiae induces the pleiotropic drug resistance stress response

Yeast ◽  
2010 ◽  
Vol 27 (12) ◽  
pp. 983-998 ◽  
Author(s):  
René Verwaal ◽  
Yang Jiang ◽  
Jing Wang ◽  
Jean-Marc Daran ◽  
Gerhard Sandmann ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Stress Response ◽  
Pleiotropic Drug Resistance ◽  
Carotenoid Production

scholarly journals Induction of multiple pleiotropic drug resistance genes in yeast engineered to produce an increased level of anti-malarial drug precursor, artemisinic acid

2008 ◽  
Vol 8 (1) ◽  
pp. 83 ◽  
Author(s):  
Dae-Kyun Ro ◽  
Mario Ouellet ◽  
Eric M Paradise ◽  
Helcio Burd ◽  
Diana Eng ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Resistance Genes ◽  
Pleiotropic Drug Resistance ◽  
Artemisinic Acid

scholarly journals RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ0 cells of Saccharomyces cerevisiae

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yoichi Yamada
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drug Resistance ◽  
Positive Response ◽  
Mrna Levels ◽  
Transporter Gene ◽  
Transcriptional Expression ◽  
Pleiotropic Drug Resistance ◽  
Wild Type ◽  
Binding Partners ◽  
Retrograde Signalling

Abstract Background In Saccharomyces cerevisiae, the retrograde signalling pathway is activated in ρ0/− cells, which lack mitochondrial DNA. Within this pathway, the activation of the transcription factor Pdr3 induces transcription of the ATP-binding cassette (ABC) transporter gene, PDR5, and causes pleiotropic drug resistance (PDR). Although a histone deacetylase, Rpd3, is also required for cycloheximide resistance in ρ0/− cells, it is currently unknown whether Rpd3 and its DNA binding partners, Ume6 and Ash1, are involved in the activation of PDR5 transcription and PDR in ρ0/− cells. This study investigated the roles of RPD3, UME6, and ASH1 in the activation of PDR5 transcription and PDR by retrograde signalling in ρ0 cells. Results ρ0 cells in the rpd3∆ and ume6∆ strains, with the exception of the ash1∆ strain, were sensitive to fluconazole and cycloheximide. The PDR5 mRNA levels in ρ0 cells of the rpd3∆ and ume6∆ strains were significantly reduced compared to the wild-type and ash1∆ strain. Transcriptional expression of PDR5 was reduced in cycloheximide-exposed and unexposed ρ0 cells of the ume6∆ strain; the transcriptional positive response of PDR5 to cycloheximide exposure was also impaired in this strain. Conclusions RPD3 and UME6 are responsible for enhanced PDR5 mRNA levels and PDR by retrograde signalling in ρ0 cells of S. cerevisiae.

Interaction of the yeast pleiotropic drug resistance genes PDR1 and PDR5

1992 ◽  
Vol 21 (6) ◽  
pp. 431-436 ◽  
Author(s):  
Shirley Meyers ◽  
Wren Schauer ◽  
Elisabetta Balzi ◽  
Marisa Wagner ◽  
Andr� Goffeau ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Resistance Genes ◽  
Pleiotropic Drug Resistance
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