scholarly journals Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes

2020 ◽  
Vol 6 (40) ◽  
pp. eabc6521 ◽  
Author(s):  
Zhongya Qin ◽  
Congping Chen ◽  
Sicong He ◽  
Ye Wang ◽  
Kam Fai Tam ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Adaptive Optics ◽  
Critical Role ◽  
Random Access ◽  
Brain Structures ◽  
Invasive Approach ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

Optical deep-brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep-brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.

scholarly journals Adaptive optics two-photon endomicroscopy enables deep brain imaging at synaptic resolution over large volumes

2020 ◽  
Author(s):  
Zhongya Qin ◽  
Congping Chen ◽  
Sicong He ◽  
Ye Wang ◽  
Kam Fai Tam ◽  
...  
Keyword(s):  
Brain Imaging ◽  
Adaptive Optics ◽  
Critical Role ◽  
Random Access ◽  
Brain Structures ◽  
Invasive Approach ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

AbstractOptical deep brain imaging in vivo at high resolution has remained a great challenge over the decades. Two-photon endomicroscopy provides a minimally invasive approach to image buried brain structures, once it is integrated with a gradient refractive index (GRIN) lens embedded in the brain. However, its imaging resolution and field of view are compromised by the intrinsic aberrations of the GRIN lens. Here, we develop a two-photon endomicroscopy by adding adaptive optics based on the direct wavefront sensing, which enables recovery of diffraction-limited resolution in deep brain imaging. A new precompensation strategy plays a critical role to correct aberrations over large volumes and achieve rapid random-access multiplane imaging. We investigate the neuronal plasticity in the hippocampus, a critical deep brain structure, and reveal the relationship between the somatic and dendritic activity of pyramidal neurons.

Adaptive optics two-photon microendoscopy for high-resolution and deep-brain imaging in vivo

2020 ◽  
Author(s):  
Congping Chen ◽  
Zhongya Qin ◽  
Sicong He ◽  
Wanjie Wu ◽  
Ye Wang ◽  
...  
Keyword(s):  
High Resolution ◽  
Brain Imaging ◽  
Adaptive Optics ◽  
Two Photon ◽  
Deep Brain

scholarly journals Dual GRIN lens two-photon endoscopy for high-speed volumetric and deep brain imaging

2020 ◽  
Vol 12 (1) ◽  
pp. 162
Author(s):  
Yu-Feng Chien ◽  
Jyun-Yi Lin ◽  
Po-Ting Yeh ◽  
Kuo-Jen Hsu ◽  
Yu-Hsuan Tsai ◽  
...  
Keyword(s):  
Brain Imaging ◽  
High Speed ◽  
Two Photon ◽  
Grin Lens ◽  
Deep Brain

scholarly journals Minimally invasive deep-brain imaging through a 50 μm-core multimode fibre

2018 ◽  
Author(s):  
Sebastian A. Vasquez-Lopez ◽  
Vadim Koren ◽  
Martin Plöschner ◽  
Zahid Padamsey ◽  
Tomáš Čižmár ◽  
...  
Keyword(s):  
High Resolution ◽  
Brain Imaging ◽  
Light Propagation ◽  
Neural Circuit ◽  
Complex Refractive Index ◽  
Brain Structures ◽  
Brain Regions ◽  
Long Distance ◽  
Deep Brain

AbstractAchieving optical access to deep-brain structures represents an important step towards the goal of understanding the mammalian central nervous system. The complex refractive index distribution within brain tissue introduces severe aberrations to long-distance light propagation thereby prohibiting image reconstruction using currently available non-invasive techniques. In an attempt to overcome this challenge endoscopic approaches have been adopted, principally in the form of fibre bundles or GRIN-lens based endoscopes. Unfortunately, these approaches create substantial mechanical lesions of the tissue precipitating neuropathological responses that include inflammation and gliosis. Together, lesions and the associated neuropathology may compromise neural circuit performance. By replacing Fourier-based image relay with a holographic approach, we have been able to reduce the volume of tissue lesion by more than 100-fold, while preserving diffraction-limited imaging performance. Here we demonstrate high-resolution fluorescence imaging of neuronal structures, dendrites and synaptic specialisations, in deep-brain regions of living mice. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.

scholarly journals High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Guanghan Meng ◽  
Yajie Liang ◽  
Sarah Sarsfield ◽  
Wan-chen Jiang ◽  
Rongwen Lu ◽  
...  
Keyword(s):  
High Throughput ◽  
Brain Structures ◽  
Optical Probe ◽  
Volumetric Imaging ◽  
Two Photon ◽  
Anatomical Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Deep Brain

Optical imaging has become a powerful tool for studying brains in vivo. The opacity of adult brains makes microendoscopy, with an optical probe such as a gradient index (GRIN) lens embedded into brain tissue to provide optical relay, the method of choice for imaging neurons and neural activity in deeply buried brain structures. Incorporating a Bessel focus scanning module into two-photon fluorescence microendoscopy, we extended the excitation focus axially and improved its lateral resolution. Scanning the Bessel focus in 2D, we imaged volumes of neurons at high-throughput while resolving fine structures such as synaptic terminals. We applied this approach to the volumetric anatomical imaging of dendritic spines and axonal boutons in the mouse hippocampus, and functional imaging of GABAergic neurons in the mouse lateral hypothalamus in vivo.

In Vivo Atlas of Deep Brain Structures

2002 ◽  
Author(s):  
Sebastiano Lucerna ◽  
Francesco M. Salpietro ◽  
Concetta Alafaci ◽  
Francesco Tomasello
Keyword(s):  
Brain Structures ◽  
Deep Brain

scholarly journals Shared and Unique Roles of Cap23 and Gap43 in Actin Regulation, Neurite Outgrowth, and Anatomical Plasticity

2000 ◽  
Vol 149 (7) ◽  
pp. 1443-1454 ◽  
Author(s):  
Dunja Frey ◽  
Thorsten Laux ◽  
Lan Xu ◽  
Corinna Schneider ◽  
Pico Caroni
Keyword(s):  
Actin Cytoskeleton ◽  
Neurite Outgrowth ◽  
Critical Role ◽  
Brain Structures ◽  
Calmodulin Binding ◽  
Essentially Normal ◽  
Anatomical Plasticity ◽  
Nerve Sprouting

CAP23 is a major cortical cytoskeleton–associated and calmodulin binding protein that is widely and abundantly expressed during development, maintained in selected brain structures in the adult, and reinduced during nerve regeneration. Overexpression of CAP23 in adult neurons of transgenic mice promotes nerve sprouting, but the role of this protein in process outgrowth was not clear. Here, we show that CAP23 is functionally related to GAP43, and plays a critical role to regulate nerve sprouting and the actin cytoskeleton. Knockout mice lacking CAP23 exhibited a pronounced and complex phenotype, including a defect to produce stimulus-induced nerve sprouting at the adult neuromuscular junction. This sprouting deficit was rescued by transgenic overexpression of either CAP23 or GAP43 in adult motoneurons. Knockin mice expressing GAP43 instead of CAP23 were essentially normal, indicating that, although these proteins do not share homologous sequences, GAP43 can functionally substitute for CAP23 in vivo. Cultured sensory neurons lacking CAP23 exhibited striking alterations in neurite outgrowth that were phenocopied by low doses of cytochalasin D. A detailed analysis of such cultures revealed common and unique functions of CAP23 and GAP43 on the actin cytoskeleton and neurite outgrowth. The results provide compelling experimental evidence for the notion that CAP23 and GAP43 are functionally related intrinsic determinants of anatomical plasticity, and suggest that these proteins function by locally promoting subplasmalemmal actin cytoskeleton accumulation.

NIR‐II Excitable Conjugated Polymer Dots with Bright NIR‐I Emission for Deep In Vivo Two‐Photon Brain Imaging Through Intact Skull

2019 ◽  
Vol 29 (15) ◽  
pp. 1808365 ◽  
Author(s):  
Shaowei Wang ◽  
Jie Liu ◽  
Guangxue Feng ◽  
Lai Guan Ng ◽  
Bin Liu
Keyword(s):  
Brain Imaging ◽  
Conjugated Polymer ◽  
Two Photon ◽  
Polymer Dots
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