scholarly journals Horizontal genome transfer by cell-to-cell travel of whole organelles

2021 ◽  
Vol 7 (1) ◽  
pp. eabd8215
Author(s):  
Alexander P. Hertle ◽  
Benedikt Haberl ◽  
Ralph Bock
Keyword(s):  
Cell Wall ◽  
Recent Work ◽  
Horizontal Transfer ◽  
Plastid Genome ◽  
Novel Species ◽  
New Combinations ◽  
Organelle Movement ◽  
Organellar Genomes ◽  
Nuclear Genomes ◽  
Intercellular Connections

Recent work has revealed that both plants and animals transfer genomes between cells. In plants, horizontal transfer of entire plastid, mitochondrial, or nuclear genomes between species generates new combinations of nuclear and organellar genomes, or produces novel species that are allopolyploid. The mechanisms of genome transfer between cells are unknown. Here, we used grafting to identify the mechanisms involved in plastid genome transfer from plant to plant. We show that during proliferation of wound-induced callus, plastids dedifferentiate into small, highly motile, amoeboid organelles. Simultaneously, new intercellular connections emerge by localized cell wall disintegration, forming connective pores through which amoeboid plastids move into neighboring cells. Our work uncovers a pathway of organelle movement from cell to cell and provides a mechanistic framework for horizontal genome transfer.

scholarly journals Lysinibacillus antri sp. nov., isolated from cave soil

2020 ◽  
Vol 70 (5) ◽  
pp. 3295-3299 ◽  
Author(s):  
Manik Prabhu Narsing Rao ◽  
Zhou-Yan Dong ◽  
Xue-Ke Niu ◽  
Kun Zhang ◽  
Ying-Qian Kang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Novel Species ◽  
Guizhou Province ◽  
Species Delineation ◽  
Gram Stain ◽  
West China ◽  
Content Type ◽  
South West ◽  
Optimum Ph ◽  
Genome Comparisons

A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China. SYSU K30002T grew at 28–40 °C (optimum, 37 °C), at pH 5.0–8.0 (optimum, pH 7.0) and in the presence of 0–4 % (w/v) NaCl (optimum in the absence of NaCl). The cell-wall peptidoglycan type was A4α (Lys–Asp). The cell-wall sugars of SYSU K30002T were ribose, galactose and mannose, and MK-7 was the menaquinone. The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. The G+C content of the genomic DNA was 36.1 mol%. The average nucleotide identity values between SYSU K30002T and its closest relatives were below the cut-off level (95–96 %) for species delineation. Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus , for which the name Lysinibacillusantri sp. nov. is proposed. The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).

scholarly journals Evolution of the chloroplast genome

2003 ◽  
Vol 358 (1429) ◽  
pp. 99-107 ◽  
Author(s):  
Christopher J. Howe ◽  
Adrian C. Barbrook ◽  
V. Lila Koumandou ◽  
R. Ellen R. Nisbet ◽  
Hamish A. Symington ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Chloroplast Genome ◽  
Genome Organization ◽  
Plastid Genome ◽  
Single Gene ◽  
Selective Advantage ◽  
Nucleotide Composition ◽  
Large Circle ◽  
Nuclear Genomes ◽  
Adenine Thymine

We discuss the suggestion that differences in the nucleotide composition between plastid and nuclear genomes may provide a selective advantage in the transposition of genes from plastid to nucleus. We show that in the adenine, thymine (AT)–rich genome of Borrelia burgdorferi several genes have an AT–content lower than the average for the genome as a whole. However, genes whose plant homologues have moved from plastid to nucleus are no less AT–rich than genes whose plant homologues have remained in the plastid, indicating that both classes of gene are able to support a high AT–content. We describe the anomalous organization of dinoflagellate plastid genes. These are located on small circles of 2–3 kbp, in contrast to the usual plastid genome organization of a single large circle of 100–200 kbp. Most circles contain a single gene. Some circles contain two genes and some contain none. Dinoflagellate plastids have retained far fewer genes than other plastids. We discuss a similarity between the dinoflagellate minicircles and the bacterial integron system.

scholarly journals Coraliomargarita akajimensis gen. nov., sp. nov., a novel member of the phylum ‘Verrucomicrobia’ isolated from seawater in Japan

2007 ◽  
Vol 57 (5) ◽  
pp. 959-963 ◽  
Author(s):  
Jaewoo Yoon ◽  
Mina Yasumoto-Hirose ◽  
Atsuko Katsuta ◽  
Hiroshi Sekiguchi ◽  
Satoru Matsuda ◽  
...  
Keyword(s):  
Cell Wall ◽  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Hard Coral ◽  
The 16S Rrna Gene

An obligately aerobic, Gram-negative, non-spore-forming, non-motile, spherical bacterium, designated strain 04OKA010-24T, was isolated from seawater surrounding the hard coral Galaxea fascicularis L., collected at Majanohama, Akajima, Japan, and was subjected to a polyphasic taxonomic study. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that the new strain represented a member of the phylum ‘Verrucomicrobia’ and shared 84–95 % sequence similarity with cultivated strains of ‘Verrucomicrobia’ subdivision 4. Amino acid analysis of the cell-wall hydrolysate indicated the absence of muramic acid and diaminopimelic acid, which suggested that the strain did not contain peptidoglycan in the cell wall. The G+C content of the DNA was 53.9 mol%. MK-7 was the major menaquinone and C14 : 0, C18 : 1 ω9c and C18 : 0 were the major fatty acids. On the basis of these data, it was concluded that strain 04OKA010-24T represents a novel species in a new genus in subdivision 4 of the phylum ‘Verrucomicrobia’, for which the name Coraliomargarita akajimensis gen. nov., sp. nov. is proposed. The type strain of Coraliomargarita akajimensis is 04OKA010-24T (=MBIC06463T=IAM 15411T=KCTC 12865T).

scholarly journals Pelagicoccus croceus sp. nov., a novel marine member of the family Puniceicoccaceae within the phylum ‘Verrucomicrobia’ isolated from seagrass

2007 ◽  
Vol 57 (12) ◽  
pp. 2874-2880 ◽  
Author(s):  
Jaewoo Yoon ◽  
Naoya Oku ◽  
Satoru Matsuda ◽  
Hiroaki Kasai ◽  
Akira Yokota
Keyword(s):  
Cell Wall ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Antibiotic Susceptibility Test ◽  
The Family ◽  
Lactam Antibiotic

An obligately aerobic, spherical, non-motile, pale-yellow pigmented bacterium was isolated from a piece of leaf of seagrass, Enhalus acoroides (L.f.) Royle, grown in Okinawa, Japan and was subjected to a polyphasic taxonomic study. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolate N5FB36-5T shared approximately 96–98 % sequence similarity with the species of the genus Pelagicoccus of the family Puniceicoccaceae within the phylum ‘Verrucomicrobia’. The DNA–DNA relatedness values of strain N5FB36-5T with Pelagicoccus mobilis 02PA-Ca-133T and Pelagicoccus albus YM14-201T were below 70 %, which is accepted as the phylogenetic definition of a novel species. β-Lactam antibiotic susceptibility test and amino acid analysis of the cell wall hydrolysates indicated the absence of muramic acid and diaminopimelic acid in the cell walls, which suggested that this strain lacks an ordinary Gram-negative type of peptidoglycan in the cell wall. The DNA G+C content of strain N5FB36-5T was 51.6 mol%; MK-7 was the major menaquinone; and the presence of C16 : 0, C16 : 1 ω7c and anteiso-C15 : 0 as the major cellular fatty acids supported the identification of the novel isolate as a member of the genus Pelagicoccus. On the basis of polyphasic taxonomic data, it was concluded that this strain should be classified as a novel species of the genus Pelagicoccus, for which the name Pelagicoccus croceus sp. nov. is proposed. The type strain is N5FB36-5T (=MBIC08282T=KCTC 12903T).

scholarly journals Planotetraspora kaengkrachanensis sp. nov. and Planotetraspora phitsanulokensis sp. nov., isolated from soil

2010 ◽  
Vol 60 (9) ◽  
pp. 2076-2081 ◽  
Author(s):  
Chanwit Suriyachadkun ◽  
Suwanee Chunhametha ◽  
Chitti Thawai ◽  
Tomohiko Tamura ◽  
Wanchern Potacharoen ◽  
...  
Keyword(s):  
Cell Wall ◽  
Type Strain ◽  
Novel Species ◽  
Aerial Mycelium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Whole Cell ◽  
Mycolic Acids ◽  
Gene Sequence Analysis

Two novel bacterial strains were isolated from tropical rain forest soil from Thailand. Strains A-T 0875T and A-T 1383T stained Gram-positive and were filamentous bacteria that developed cylindrical sporangia containing four oval- to rod-shaped spores at the ends of short sporangiophores on branched aerial mycelium. The cell-wall peptidoglycan contained meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; whole-cell hydrolysates contained rhamnose, madurose, glucose, galactose and 3-O-methylmannose as whole-cell sugars. The predominant menaquinone was MK-9(H4). Mycolic acids were not detected. The diagnostic phospholipid was phosphatidylethanolamine. The predominant cellular fatty acids were iso-C16 : 0 and 10-methyl-C17 : 0. For both strains, the G+C content of the genomic DNA was 71 mol%. Phenotypic and chemotaxonomic analyses showed that the characteristics of the two isolates were typical of members of the genus Planotetraspora. Furthermore, 16S rRNA gene sequence analysis also indicated that the strains belonged to the genus Planotetraspora but as representatives of two novel species. Following an evaluation of our phenotypic, chemotaxonomic and genotypic studies, two novel species are proposed, Planotetraspora kaengkrachanensis sp. nov. (type strain A-T 0875T=BCC 24832T=NBRC 104272T) and Planotetraspora phitsanulokensis sp. nov. (type strain A-T 1383T=BCC 26045T=NBRC 104273T).

scholarly journals Agromyces soli sp. nov., isolated from farm soil

2011 ◽  
Vol 61 (6) ◽  
pp. 1286-1292 ◽  
Author(s):  
Myungjin Lee ◽  
Leonid N. Ten ◽  
Sung-Geun Woo ◽  
Joonhong Park
Keyword(s):  
Cell Wall ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Unidentified Phospholipid ◽  
Cellular Fatty Acids ◽  
Gene Sequence Analysis

A Gram-positive, aerobic to microaerophilic, non-motile bacterial strain, designated MJ21T, was isolated from farm soil and was characterized to determine its taxonomic position by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain MJ21T was placed within the genus Agromyces, and exhibited relatively high levels of similarity to Agromyces ulmi XIL01T (97.8 %), Agromyces aurantiacus YIM 21741T (97.1 %), Agromyces mediolanus JCM 3346T (96.7 %), A. mediolanus JCM 1376 (99.1 %), A. mediolanus JCM 9632 (99.1 %), A. mediolanus JCM 9633 (98.9 %) and A. mediolanus JCM 9631 (96.5 %). Chemotaxonomic data also supported the classification of strain MJ21T within the genus Agromyces. The new isolate contained MK-12 as the predominant menaquinone and rhamnose, galactose and xylose as cell-wall sugars. The major cellular fatty acids (>10 % of the total) were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. Cell-wall amino acids were 2,4-diaminobutyric acid, glutamic acid, glycine and alanine. Diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids and one unidentified phospholipid were detected as polar lipids. The DNA G+C content of strain MJ21T was 73.4 mol%. However, levels of DNA–DNA relatedness between strain MJ21T and the seven phylogenetically closest Agromyces strains ranged from 14 to 56 %, showing clearly that the new isolate represents a novel genomic species. Strain MJ21T could be differentiated clearly from its phylogenetic neighbours on the basis of phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ21T is considered to represent a novel species of the genus Agromyces, for which the name Agromyces soli sp. nov. is proposed. The type strain is MJ21T ( = KCTC 19549T  = JCM 16247T).

scholarly journals Exiguobacterium Sp. HA2, isolated from the Ilam Mountains of Iran

2021 ◽  
Author(s):  
Reza Heidari ◽  
Mostafa Akbariqomi ◽  
Gholamreza Tavoosidana ◽  
Garshasb Rigi
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Novel Species ◽  
Isoprenoid Quinone ◽  
Gram Stain ◽  
Phenotypic Characteristics ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Peptidoglycan Layer ◽  
Major Isoprenoid Quinone

ABSTRACTA motile, Gram-stain-positive, rod-shaped, non-sporing, tolerate up to 5% NaCl, grew at 0–25 °C, designated Exiguobacterium sp. HA2 was isolated from the soil of the Ilam Mountains of Iran during October 2016. The major isoprenoid quinone is MK-7 and in the smaller amount are MK-6 and MK-8. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine. Major fatty acids (>10 %) are isoC13:0, isoC15:0 and C16:0. The bacterial cell wall peptidoglycan layer was lysine-glycine. The 16S rRNA sequence was analyzed at the phylogenetic levels. Also, A supplemental comparison was made between five other genes including csp, gyrB, hsp70, rpoB, and citC. According to the results of genotypic and phenotypic characteristics, the strain was categorized in the genus Exiguobacterium. This bacterium had the closest relation with Exiguobacterium undae, and thus was dubbed Exiguobacterium sp. HA2. The different in the Phenotypic, functional characteristics and phylogenetic indicated Exiguobacterium sp. HA2 can be regarded as representing considered a novel species within the genus Exiguobacterium.

scholarly journals Horizontal Transfer of DNA from the Mitochondrial to the Plastid Genome and Its Subsequent Evolution in Milkweeds (Apocynaceae)

2013 ◽  
Vol 5 (10) ◽  
pp. 1872-1885 ◽  
Author(s):  
Shannon C.K. Straub ◽  
Richard C. Cronn ◽  
Christopher Edwards ◽  
Mark Fishbein ◽  
Aaron Liston
Keyword(s):  
Horizontal Transfer ◽  
Plastid Genome ◽  
Subsequent Evolution

Algae in association with heterotrophic or holozoic organisms

1952 ◽  
Vol 139 (895) ◽  
pp. 185-192 ◽  
Keyword(s):  
Cell Wall ◽  
Recent Work ◽  
Marine Invertebrates ◽  
Algal Cell ◽  
Organic Food ◽  
The Past ◽  
Invertebrate Animal ◽  
Widespread Phenomenon ◽  
The Subject

The association of an alga capable of photosynthesis with another organism dependent on a supply of organic food, be it another alga, a fungus, or an invertebrate animal, is a very widespread phenomenon. The algae concerned are usually unicellular or simple filamentous forms, and, apart from the Zooxanthellae inhabiting marine invertebrates, are for the most part members of the Chlorophyceae or Myxophyceae. My theme is a very wide one, and it will therefore be necessary to confine my remarks to certain selected examples. The combination of alga and fungus to constitute a lichen has been the subject of repeated investigation and has been variously interpreted. Whilst in the past penetration of the algal cells, or gonidia as they are commonly called, by fungal haustoria had only been rarely reported and was regarded by many as something quite unusual, if not abnormal, the more recent work of Fry (1928), and especially that of Geitler (1933, 1934, 1937, 1938) and of his pupil Tschermak (1941, 1943), has shown that it is comparatively common. In some lichens the haustoria penetrate deeply into the algal cells, seemingly occupying an invagination of the protoplast, but not necessarily leading to its destruction. In heteromerous lichens it is more usual to find what have been called intramembranous haustoria which only penetrate through the cell wall up to the protoplast of the algal cell. Haustoria of this type are formed during the summer months and may be discoverable only for a comparatively brief period.

An interaction between lysozyme and penicillin

1967 ◽  
Vol 167 (1009) ◽  
pp. 439-440 ◽  
Keyword(s):  
Cell Wall ◽  
Recent Work ◽  
Bacterial Cell ◽  
Structural Similarity ◽  
Antibiotic Activity ◽  
Bacterial Cell Wall ◽  
Cross Linking ◽  
Cell Wall Synthesis ◽  
Knowing That ◽  
Reactive Groups

Collins & Richmond (1962) have drawn attention to the close structural similarity between the reactive groups of penicillin and the reactive groups of N -acetylmuramic acid and, knowing that penicillin interferes with the synthesis of the bacterial cell wall, they have suggested that the antibiotic activity might possibly be explained in terms of a confusion between these two molecules by the cell wall synthesizing enzymes. Although recent work (Anderson, Matsukashi, Haskin & Strominger 1965; Wise & Park 1965; Tipper & Strominger 1965) has shown that penicillin appears to act by blocking the peptide cross-linking stage of bacterial cell wall synthesis rather than the polysaccharide polymerization stage, we wondered if lysozyme might bind penicillin purely on the basis of its structural similarity to N -acetylmuramic acid, a molecule for which lysozyme must have a specificity since it is part of the substrate of lysozyme.

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