Pou5f1 Transcription Factor Controls Zygotic Gene Activation In Vertebrates

Science ◽  
2013 ◽  
Vol 341 (6149) ◽  
pp. 1005-1009 ◽  
Author(s):  
Manuel Leichsenring ◽  
Julia Maes ◽  
Rebecca Mössner ◽  
Wolfgang Driever ◽  
Daria Onichtchouk
Keyword(s):  
Transcription Factor ◽  
Binding Sites ◽  
Gene Activation ◽  
Gene Products ◽  
Developmental Control ◽  
Zygotic Transcription ◽  
Maternal To Zygotic Transition ◽  
Zygotic Gene ◽  
Zygotic Gene Activation ◽  
Maternal Gene

The development of multicellular animals is initially controlled by maternal gene products deposited in the oocyte. During the maternal-to-zygotic transition, transcription of zygotic genes commences, and developmental control starts to be regulated by zygotic gene products. In Drosophila, the transcription factor Zelda specifically binds to promoters of the earliest zygotic genes and primes them for activation. It is unknown whether a similar regulation exists in other animals. We found that zebrafish Pou5f1, a homolog of the mammalian pluripotency transcription factor Oct4, occupies SOX-POU binding sites before the onset of zygotic transcription and activates the earliest zygotic genes. Our data position Pou5f1 and SOX-POU sites at the center of the zygotic gene activation network of vertebrates and provide a link between zygotic gene activation and pluripotency control.

scholarly journals Minor zygotic gene activation is essential for mouse preimplantation development

2018 ◽  
Vol 115 (29) ◽  
pp. E6780-E6788 ◽  
Author(s):  
Ken-ichiro Abe ◽  
Satoshi Funaya ◽  
Dai Tsukioka ◽  
Machika Kawamura ◽  
Yutaka Suzuki ◽  
...  
Keyword(s):  
Dna Replication ◽  
Gene Activation ◽  
Marked Change ◽  
Cell Stage ◽  
Maternal Mrna ◽  
Maternal To Zygotic Transition ◽  
Before And After ◽  
H3k4me3 Mark ◽  
Zygotic Gene ◽  
Zygotic Gene Activation

In mice, transcription initiates at the mid-one-cell stage and transcriptional activity dramatically increases during the two-cell stage, a process called zygotic gene activation (ZGA). Associated with ZGA is a marked change in the pattern of gene expression that occurs after the second round of DNA replication. To distinguish ZGA before and after the second-round DNA replication, the former and latter are called minor and major ZGA, respectively. Although major ZGA are required for development beyond the two-cell stage, the function of minor ZGA is not well understood. Transiently inhibiting minor ZGA with 5, 6-dichloro-1-β-d-ribofuranosyl-benzimidazole (DRB) resulted in the majority of embryos arresting at the two-cell stage and retention of the H3K4me3 mark that normally decreases. After release from DRB, at which time major ZGA normally occurred, transcription initiated with characteristics of minor ZGA but not major ZGA, although degradation of maternal mRNA normally occurred. Thus, ZGA occurs sequentially starting with minor ZGA that is critical for the maternal-to-zygotic transition.

scholarly journals Low-affinity transcription factor binding sites shape morphogen responses and enhancer evolution

2013 ◽  
Vol 368 (1632) ◽  
pp. 20130018 ◽  
Author(s):  
Andrea I. Ramos ◽  
Scott Barolo
Keyword(s):  
Transcription Factor ◽  
Binding Affinity ◽  
Binding Sites ◽  
Target Genes ◽  
Gene Activation ◽  
Transcriptional Response ◽  
Morphogen Gradients ◽  
Weak Binding ◽  
Initial Hypothesis

In the era of functional genomics, the role of transcription factor (TF)–DNA binding affinity is of increasing interest: for example, it has recently been proposed that low-affinity genomic binding events, though frequent, are functionally irrelevant. Here, we investigate the role of binding site affinity in the transcriptional interpretation of Hedgehog (Hh) morphogen gradients . We noted that enhancers of several Hh-responsive Drosophila genes have low predicted affinity for Ci, the Gli family TF that transduces Hh signalling in the fly. Contrary to our initial hypothesis, improving the affinity of Ci/Gli sites in enhancers of dpp , wingless and stripe , by transplanting optimal sites from the patched gene, did not result in ectopic responses to Hh signalling. Instead, we found that these enhancers require low-affinity binding sites for normal activation in regions of relatively low signalling. When Ci/Gli sites in these enhancers were altered to improve their binding affinity, we observed patterning defects in the transcriptional response that are consistent with a switch from Ci-mediated activation to Ci-mediated repression. Synthetic transgenic reporters containing isolated Ci/Gli sites confirmed this finding in imaginal discs. We propose that the requirement for gene activation by Ci in the regions of low-to-moderate Hh signalling results in evolutionary pressure favouring weak binding sites in enhancers of certain Hh target genes.

scholarly journals Spatiotemporal Cascade of Transcription Factor Binding Required for Promoter Activation

2014 ◽  
Vol 35 (4) ◽  
pp. 688-698 ◽  
Author(s):  
Robert M. Yarrington ◽  
Jared S. Rudd ◽  
David J. Stillman
Keyword(s):  
Transcription Factor ◽  
Transcriptional Activation ◽  
Binding Sites ◽  
Gene Activation ◽  
Regulatory Sequence ◽  
Factor Binding ◽  
Multiple Binding Sites ◽  
Dependent Cell ◽  
The Right

Promoters often contain multiple binding sites for a single factor. The yeastHOgene contains nine highly conserved binding sites for the SCB (Swi4/6-dependent cell cycle box) binding factor (SBF) complex (composed of Swi4 and Swi6) in the 700-bp upstream regulatory sequence 2 (URS2) promoter region. Here, we show that the distal and proximal SBF sites in URS2 function differently. Chromatin immunoprecipitation (ChIP) experiments show that SBF binds preferentially to the left side of URS2 (URS2-L), despite equivalent binding to the left-half and right-half SBF sitesin vitro. SBF binding at URS2-L sites depends on prior chromatin remodeling events at the upstream URS1 region. These signals from URS1 influence chromatin changes at URS2 but only at sites within a defined distance. SBF bound at URS2-L, however, is unable to activate transcription but instead facilitates SBF binding to sites in the right half (URS2-R), which are required for transcriptional activation. Factor binding atHO, therefore, follows a temporal cascade, with SBF bound at URS2-L serving to relay a signal from URS1 to the SBF sites in URS2-R that ultimately activate gene expression. Taken together, we describe a novel property of a transcription factor that can have two distinct roles in gene activation, depending on its location within a promoter.

scholarly journals Minor Splicing Factors Zrsr1 and Zrsr2 Are Essential for Early Embryo Development and 2-Cell-Like Conversion

2020 ◽  
Vol 21 (11) ◽  
pp. 4115 ◽  
Author(s):  
Isabel Gómez-Redondo ◽  
Priscila Ramos-Ibeas ◽  
Eva Pericuesta ◽  
Raúl Fernández-González ◽  
Ricardo Laguna-Barraza ◽  
...  
Keyword(s):  
Stem Cells ◽  
Alternative Splicing ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Gene Activation ◽  
Early Embryo ◽  
Early Embryo Development ◽  
Zygotic Gene ◽  
Zygotic Gene Activation ◽  
Induced Pluripotent

Minor splicing plays an important role in vertebrate development. Zrsr1 and Zrsr2 paralog genes have essential roles in alternative splicing, mainly participating in the recognition of minor (U12) introns. To further explore their roles during early embryo development, we produced Zrsr1mu and Zrsr2mu mutant mice, containing truncating mutations within the second zinc finger domain. Both homozygous mutant mice were viable with a normal lifespan. When we crossed a homozygous Zrsr2mu/mu female with Zrsr1mu/mu male, the double heterozygotes were non-viable, giving rise to embryos that stopped developing mainly between the 2- and 4-cell stages, just after zygotic gene activation. RNA-seq analysis of Zrsr1/2mu 2-cell embryos showed altered gene and isoform expression of thousands of genes enriched in gene ontology terms and biological pathways related to ribosome, RNA transport, spliceosome, and essential zygotic gene activation steps. Alternative splicing was analyzed, showing a significant increase in intron retention in both U2 and U12 intron-containing genes related to cell cycle and mitotic nuclear division. Remarkably, both Zrsr1 and Zrsr2 were required for the conversion of mouse-induced pluripotent stem cells into 2C-like cells. According to our results, Zrsr1 or Zrsr2 are necessary for ZGA and both are indispensable for the conversion of induced pluripotent stem cells into 2C-like cells.

scholarly journals Zygotic Gene Activation and Maternal Factors in Mammals

2007 ◽  
Vol 53 (4) ◽  
pp. 707-715 ◽  
Author(s):  
Naojiro MINAMI ◽  
Toru SUZUKI ◽  
Satoshi TSUKAMOTO
Keyword(s):  
Gene Activation ◽  
Maternal Factors ◽  
Zygotic Gene ◽  
Zygotic Gene Activation

scholarly journals Paternal H3K4 methylation is required for minor zygotic gene activation and early mouse embryonic development

EMBO Reports ◽  
2015 ◽  
Vol 16 (7) ◽  
pp. 803-812 ◽  
Author(s):  
Keisuke Aoshima ◽  
Erina Inoue ◽  
Hirofumi Sawa ◽  
Yuki Okada
Keyword(s):  
Embryonic Development ◽  
Gene Activation ◽  
H3k4 Methylation ◽  
Zygotic Gene ◽  
Zygotic Gene Activation ◽  
Early Mouse
Sign in / Sign up

Export Citation Format

Share Document