Mesoderm induction in amphibians: the role of TGF-beta 2-like factors

Science ◽  
1988 ◽  
Vol 239 (4841) ◽  
pp. 783-785 ◽  
Author(s):  
F Rosa ◽  
A. Roberts ◽  
D Danielpour ◽  
L. Dart ◽  
M. Sporn ◽  
...  
Keyword(s):  
Mesoderm Induction ◽  
Tgf Beta ◽  
Beta 2

Role of TGF beta s and BMPs as signals controlling the position of the digits and the areas of interdigital cell death in the developing chick limb autopod

Development ◽  
1996 ◽  
Vol 122 (8) ◽  
pp. 2349-2357 ◽  
Author(s):  
Y. Ganan ◽  
D. Macias ◽  
M. Duterque-Coquillaud ◽  
M.A. Ros ◽  
J.M. Hurle
Keyword(s):  
Gene Expression ◽  
Cell Death ◽  
Ectopic Expression ◽  
Local Administration ◽  
Tgf Beta ◽  
Signal Characteristic ◽  
Beta 2 ◽  
Interdigital Cell Death ◽  
Ectopic Area

The establishment of the digital rays and the interdigital spaces in the developing limb autopod is accompanied by the occurrence of corresponding domains of expression of TGF beta s and BMPs. This study analyzes whether these coincident events are functionally correlated. The experiments consisted of local administration of TGF beta-1, TGF beta-2 or BMP-4 by means of heparin or Affi-gel blue beads to the chick limb autopod in the stages preceding the onset of interdigital cell death. When beads bearing either TGF beta-1 or −2 were implanted in the interdigits, the mesodermal cells were diverted from the death program forming ectopic cartilages or extra digits in a dose- and stage-dependent fashion. This change in the interdigital phenotype was preceded by a precocious ectopic expression of ck-erg gene around the bead accompanied by down-regulation of bmp-4, msx-1 and msx-2 gene expression. When BMP-beads were implanted in the interdigital spaces, programmed cell death and the freeing of the digits were both accelerated. Implantation of beads bearing BMP-4 at the tip of the growing digits was followed by digit bifurcation, accompanied by the formation of an ectopic area of cell death resembling an extra interdigit, both morphologically and molecularly. The death-inducing effect of the BMP beads and the chondrogenic-inducing effect of the TGF beta beads were antagonized by the implantation of an additional bead preabsorbed with FGF-2, which constitutes a signal characteristic of the progress zone. It is concluded that the spatial distribution of digital rays and interdigital spaces might be controlled by a patterned distribution of TGF beta s and BMPs in the mesoderm subjacent to the progress zone.

Differential role of TGF[beta]2 during follicle activation and preantral follicle growth

2014 ◽  
Author(s):  
Elizabeth Oliver ◽  
Mhairi Laird ◽  
Stephen Franks ◽  
Kate Hardy
Keyword(s):  
Tgf Beta ◽  
Preantral Follicle ◽  
Follicle Growth ◽  
Beta 2 ◽  
Follicle Activation

Alterations in somite patterning of Myf-5-deficient mice: a possible role for FGF-4 and FGF-6

Development ◽  
1996 ◽  
Vol 122 (1) ◽  
pp. 141-150 ◽  
Author(s):  
S. Grass ◽  
H.H. Arnold ◽  
T. Braun
Keyword(s):  
Potential Role ◽  
Perinatal Death ◽  
Close Contact ◽  
Substantial Reduction ◽  
Tgf Beta ◽  
Targeted Mutation ◽  
Myogenic Factor ◽  
Beta 2 ◽  
Deficient Mice

Mice carrying a targeted mutation in the gene for the myogenic factor Myf-5 fail to form major parts of the ribs, which leads to an unstable thorax and perinatal death. Here, we report that somites of Myf-5-deficient mice lack the expression of FGF-4 and FGF-6 while TGF beta-2 is expressed normally. Early sclerotomal markers, such as Pax-1 reveal no substantial reduction of sclerotome size. At E11.5 the condensing mesenchyme of the rib anlagen is considerably reduced in size in Myf-5 mutant mice. This may be caused by the lack of Myf-5-positive, FGF-expressing cells which normally are in close contact with the lateral sclerotome generating the rib progenitors. The potential role of FGFs and TGF beta on sclerotome formation is demonstrated in micromass cultures of early somites. Combinations of FGF-4 or FGF-6 with TGF beta-2 potentiate chondrogenesis suggesting that these growth factors emanating from early myotomal and dermomyotomal cells may have instructive or permissive effects on differentiation or outgrowth of sclerotomal cells.

P11 The Role of Smooth Muscle Integrin Alpha V and TGF-Beta Pathways in Vascular Fibrosis

2020 ◽  
Vol 25 (Supplement 1) ◽  
pp. S54
Author(s):  
Alexandre Raoul ◽  
Ekaterina Belozertseva ◽  
Huguette Louis ◽  
Zhenlin Li ◽  
Veronique Regnault ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Tgf Beta

scholarly journals The potential regulatory role of hsa_circ_0004104 in the persistency of atrial fibrillation by promoting cardiac fibrosis via TGF-β pathway

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yuanfeng Gao ◽  
Ye Liu ◽  
Yuan Fu ◽  
Qianhui Wang ◽  
Zheng Liu ◽  
...  
Keyword(s):  
Cardiac Fibrosis ◽  
Expression Patterns ◽  
In Silico Analysis ◽  
Significant Negative Correlation ◽  
Circular Rnas ◽  
Tgf Beta ◽  
Rt Pcr ◽  
Derivation Cohort ◽  
Tgf Β1

Abstract Introduction The progression of paroxysmal AF (PAF) to persistent AF (PsAF) worsens the prognosis of AF, but its underlying mechanisms remain elusive. Recently, circular RNAs (circRNAs) were reported to be associated with cardiac fibrosis. In case of the vital role of cardiac fibrosis in AF persistency, we hypothesis that circRNAs may be potential regulators in the process of AF progression. Materials and methods 6 persistent and 6 paroxysmal AF patients were enrolled as derivation cohort. Plasma circRNAs expressions were determined by microarray and validated by RT-PCR. Fibrosis level, manifested by serum TGF-β, was determined by ELISA. Pathways and related non-coding RNAs involving in the progression of AF regulated were predicted by in silico analysis. Results PsAF patients showed a distinct circRNAs expression profile with 92 circRNAs significantly dysregulated (fold change ≥ 2, p < 0.05), compared with PAF patients. The validity of the expression patterns was subsequently validated by RT-PCR in another 60 AF patients (30 PsAF and PAF, respectively). In addition, all the 5 up and down regulated circRNAs were clustered in MAPK and TGF-beta signaling pathway by KEGG pathway analysis. Among the 5 circRNAs, hsa_circ_0004104 was consistently downregulated in PsAF group (0.6 ± 0.33 vs 1.46 ± 0.41, p < 0.001) and predicted to target several AF and/or cardiac fibrosis related miRNAs reported by previous studies. In addition, TGF-β1 level was significantly higher in the PsAF group (5560.23 ± 1833.64 vs 2236.66 ± 914.89, p < 0.001), and hsa_circ_0004104 showed a significant negative correlation with TGF-β1 level (r = − 0.797, p < 0.001). Conclusion CircRNAs dysregulation plays vital roles in AF persistency. hsa_circ_0004104 could be a potential regulator and biomarker in AF persistency by promoting cardiac fibrosis via targeting MAPK and TGF-beta pathways.

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