Interrogating CD8+ T cell reactivity on a genome-wide scale

2019 ◽  
Vol 11 (506) ◽  
pp. eaaz0302
Author(s):  
Kamila Naxerova
Keyword(s):  
T Cell ◽  
Large Scale ◽  
Cd8 T Cell ◽  
Cell Reactivity ◽  
Human T Cell ◽  
Genome Wide ◽  
A Genome ◽  
T Cell Antigens ◽  
Wide Scale ◽  
T Cell Reactivity

A new method enables large-scale identification of human T cell antigens.

scholarly journals Negligible impact of SARS-CoV-2 variants on CD4+ and CD8+ T cell reactivity in COVID-19 exposed donors and vaccinees.

2021 ◽  
Author(s):  
Alison Tarke ◽  
John Sidney ◽  
Nils Methot ◽  
Yun Zhang ◽  
Jennifer M Dan ◽  
...  
Keyword(s):  
T Cell ◽  
Immune Responses ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Comprehensive Analysis ◽  
T Cell Epitopes ◽  
Cell Reactivity ◽  
Cell Responses ◽  
Adaptive Immune ◽  
T Cell Reactivity

The emergence of SARS-CoV-2 variants highlighted the need to better understand adaptive immune responses to this virus. It is important to address whether also CD4+ and CD8+ T cell responses are affected, because of the role they play in disease resolution and modulation of COVID-19 disease severity. Here we performed a comprehensive analysis of SARS-CoV-2-specific CD4+ and CD8+ T cell responses from COVID-19 convalescent subjects recognizing the ancestral strain, compared to variant lineages B.1.1.7, B.1.351, P.1, and CAL.20C as well as recipients of the Moderna (mRNA-1273) or Pfizer/BioNTech (BNT162b2) COVID-19 vaccines. Similarly, we demonstrate that the sequences of the vast majority of SARS-CoV-2 T cell epitopes are not affected by the mutations found in the variants analyzed. Overall, the results demonstrate that CD4+ and CD8+ T cell responses in convalescent COVID-19 subjects or COVID-19 mRNA vaccinees are not substantially affected by mutations found in the SARS-CoV-2 variants.

scholarly journals Rescue of memory CD8+ T cell reactivity in peptide/TLR9 ligand immunization by codelivery of cytokines or CD40 ligation

Virology ◽  
2005 ◽  
Vol 331 (1) ◽  
pp. 151-158 ◽  
Author(s):  
Felix N. Toka ◽  
Małgorzata Gieryńska ◽  
Susmit Suvas ◽  
Stephen P. Schoenberger ◽  
Barry T. Rouse
Keyword(s):  
T Cell ◽  
Cd8 T Cell ◽  
Cd40 Ligation ◽  
Cell Reactivity ◽  
Tlr9 Ligand ◽  
Memory Cd8 T Cell ◽  
T Cell Reactivity

scholarly journals P55. In situ molecular assessment of CD8+ T cell reactivity in NSCLC

2006 ◽  
Vol 4 (6) ◽  
pp. 47
Author(s):  
A. Trojan ◽  
M. Montemurro ◽  
R.A. Stahel ◽  
D. Jäger
Keyword(s):  
T Cell ◽  
Cd8 T Cell ◽  
Cell Reactivity ◽  
Molecular Assessment ◽  
T Cell Reactivity

scholarly journals Profound CD8 T cell responses towards the SARS-CoV-2 ORF1ab in COVID-19 patients

2020 ◽  
Author(s):  
Anastasia Gangaev ◽  
Steven L. C. Ketelaars ◽  
Sanne Patiwael ◽  
Anna Dopler ◽  
Olga I. Isaeva ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
T Cell Response ◽  
Clinical Activity ◽  
Cell Reactivity ◽  
T Cell Recognition ◽  
Cell Responses ◽  
T Cell Reactivity ◽  
Very High

Abstract A large global effort is currently ongoing to develop vaccines against SARS-CoV-2, the causative agent of COVID-19. While there is accumulating evidence on the antibody response against SARS-CoV-2, little is known about the SARS-CoV-2 antigens that are targeted by CD8 T cells. To address this issue, we have analyzed samples from 20 COVID-19 patients for T cell recognition of 500 predicted MHC class I epitopes. CD8 T cell reactivity against SARS-CoV- 2 was common. Remarkably, a substantial fraction of the observed CD8 T cell responses were directed towards the ORF1ab polyprotein 1ab, and these CD8 T cell responses were frequently of a very high magnitude. The fact that a major part of the SARS-CoV-2 specific CD8 T cell response is directed against a part of the viral genome that is not included in the majority of vaccine candidates currently in development may potentially influence their clinical activity and toxicity profile.

Cis-regulatory elements and human evolution

2014 ◽  
Author(s):  
Adam Siepel ◽  
Leonardo Arbiza
Keyword(s):  
Transcriptional Regulation ◽  
Human Evolution ◽  
Large Scale ◽  
Regulatory Elements ◽  
Data Sets ◽  
Regulatory Evolution ◽  
Genome Wide ◽  
A Genome ◽  
Wide Scale ◽  
Human Polymorphism

Modification of gene regulation has long been considered an important force in human evolution, particularly through changes to cis-regulatory elements (CREs) that function in transcriptional regulation. For decades, however, the study of cis-regulatory evolution was severely limited by the available data. New data sets describing the locations of CREs and genetic variation within and between species have now made it possible to study CRE evolution much more directly on a genome-wide scale. Here, we review recent research on the evolution of CREs in humans based on large-scale genomic data sets. We consider inferences based on primate divergence,human polymorphism, and combinations of divergence and polymorphism. We then consider "new frontiers" in this field stemming from recent research on transcriptional regulation.

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