scholarly journals New Integron-Associated Gene Cassette Encoding a Trimethoprim-Resistant DfrB-Type Dihydrofolate Reductase

2006 ◽  
Vol 50 (8) ◽  
pp. 2863-2865 ◽  
Author(s):  
Renee S. Levings ◽  
Diane Lightfoot ◽  
Liam D. H. Elbourne ◽  
Steven P. Djordjevic ◽  
Ruth M. Hall
Keyword(s):  
Dihydrofolate Reductase ◽  
Salmonella Enterica ◽  
Gene Cassette ◽  
Antibiotic Resistant ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Type Gene

ABSTRACT A sixth gene cassette containing a dfrB-type gene, dfrB6, was found in a dfrB6-aadA1 cassette array in class 1 integrons. This array was isolated from several multiply antibiotic-resistant Salmonella enterica serovar Infantis strains that appear to be clonally related. The DfrB6 dihydrofolate reductase conferred resistance to trimethoprim.

Antibiotic resistance profile of Salmonella enterica isolated from exotic and indigenous leafy green vegetables in Accra, Ghana

2021 ◽  
Author(s):  
Jinru Chen ◽  
Joycelyn Quansah
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Gene Cassette ◽  
Nucleotide Sequencing ◽  
Dilution Method ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Resistance Profile ◽  
Antibiotic Resistance Profile ◽  
Class 1

Fresh produce-borne enteric bacterial pathogens with resistance to antibiotics have posed serious challenges to food safety and public health worldwide.  This study examined the antibiotic resistance profile of Salmonella enterica (n=33), previously isolated from exotic and indigenous leafy green vegetable samples (n=328) collected from 50 vegetable farms in 12 farming areas and 37 vegetable sellers in 4 market centers in Accra, Ghana during the period of March 2016 to March 2017, and determined the distribution of integrons among antibiotic-resistant isolates.  The susceptibility of the Salmonella isolates to 12 antibiotics was assayed using the standard disc diffusion assay.  The minimum inhibitory concentrations (MICs) of the five most resisted antibiotics were determined using the twofold macro dilution method.  PCR assay was used to detect the presence of integrons in Salmonella cells, and PCR product with amplified integron gene cassette was purified and sequenced using the Sanger sequencing technology.  The Salmonella isolates used in the study resisted at least one tested antibiotic, and multi-drug resistant (MDR) isolates were 30.3% (10/33).  Most isolates (81.8%) were resistant to sulfisoxazole.  The MICs of tetracycline, cefoxitin, streptomycin, ampicillin, and sulfisoxazole were 16, 32, 64, 64, and > 1,024 µg/ml, respectively.  A total of five different patterns of MDR were observed among the Salmonella isolates, and the common MDR patterns were AAuFox (30.3%) and AAuFoxSSu (18.1%).  One out of the 33 (3.0%) Salmonella isolates tested positive for class 1 integron with a gene cassette of about 800 bp.  Nucleotide sequencing revealed the class 1 integron carried a single gene dfrA7 .  Future studies are needed to confirm whether the consumption of contaminated leafy green vegetables is a route of acquiring antibiotic-resistant Salmonella by consumers in Accra, Ghana.

Antibiotic selective pressure for the maintenance of antibiotic resistant genes in coliform bacteria isolated from the aquatic environment

2003 ◽  
Vol 47 (3) ◽  
pp. 249-253 ◽  
Author(s):  
J.C. Park ◽  
J.C. Lee ◽  
J.Y. Oh ◽  
Y.W. Jeong ◽  
J.W. Cho ◽  
...  
Keyword(s):  
Aquatic Environment ◽  
Selective Pressure ◽  
Functional Class ◽  
Coliform Bacteria ◽  
Antibiotic Resistant ◽  
Gene Cassettes ◽  
Class 1 Integrons ◽  
Selective Pressures ◽  
Class 1 ◽  
Inti1 Gene

Coliform bacteria isolated from the aquatic environment were investigated for antibiotic susceptibility and detailed structures of class 1 integrons. A high proportion of isolates were found to be resistant to sulfamethoxazole, aminoglycosides, and β-lactams. The 750 (53.6%) isolates were resistant to one or more of the antibiotics tested out of 1,400 coliform bacteria. Based on the MIC of antibiotics and antibiogram, 150 isolates were selected and further studied for class 1 integrons. The intI1 gene was found in 36 (24.0%) of the 150 isolates. Twelve isolates carried the gene cassettes responsible for antibiotic resistance, while no gene cassettes were found in 24 isolates. Seven different genes, dfrA5, dfrA7, dfrA12, dfrA17, aaA2, aaA5, and aad(3’), were detected in gene cassettes. The dfrA and aad genes located on class 1 integrons were responsible for resistance to trimethoprim and aminoglycosides. The remaining 24 coliform bacteria had the incomplete or non-functional class 1 integrons. These results indicated that antibiotic selective pressures may play an important role to maintain gene cassettes of class 1 integrons and in the absence of sustained antibiotic pressures, such as the aquatic environment, coliform bacteria may carry empty or non-functional class 1 integrons.

PREVALENCE OF CLASS 1 INTEGRONS IN CLINICAL ISOLATES OF NON-TYPHOIDAL $SALMONELLA~ENTERICA$ CIRCULATED IN ARMENIA

2021 ◽  
Vol 55 (1 (254)) ◽  
pp. 58-66
Author(s):  
Magdalina K. Zakharyan
Keyword(s):  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Clinical Significance ◽  
Antimicrobial Therapy ◽  
Salmonella Enterica ◽  
Clinical Isolates ◽  
Class 1 Integron ◽  
Class 1 Integrons ◽  
High Prevalence ◽  
Class 1

A total of 182 non-typhoid Salmonella enterica (NTS) isolates recovered from patients between 1996 and 2014 were included in the current study focused on class 1 integron detection and its association with multidrug resistance (MDR) phenotype. A high prevalence of isolates displaying MDR and penta-resistance (resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide, tetracycline) phenotypes of clinical significance was revealed. Serotype-specific prevalence of antimicrobial resistance as well as class 1 integrons and inserted variable segments was detected in isolates. The results indicated the limitations of current antimicrobial therapy to control infections caused by MDR isolates of NTS, especially belonging to serotype Typhimurium.

scholarly journals A Predominant Multidrug-Resistant Salmonella enterica Serovar Saintpaul Clonal Line in German Turkey and Related Food Products

2010 ◽  
Vol 76 (11) ◽  
pp. 3657-3667 ◽  
Author(s):  
Janine Beutlich ◽  
Irene Rodr�guez ◽  
Andreas Schroeter ◽  
Annemarie K�sbohrer ◽  
Reiner Helmuth ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Food Products ◽  
Gene Cassette ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Clonal Line ◽  
Class 1 Integron ◽  
Pathogenic Potential ◽  
Class 1 ◽  
Plasmid Profiling

ABSTRACT Recently, Salmonella enterica subsp. enterica serovar Saintpaul has increasingly been observed in several countries, including Germany. However, the pathogenic potential and epidemiology of this serovar are not very well known. This study describes biological attributes of S. Saintpaul isolates obtained from turkeys in Germany based on characterization of their pheno- and genotypic properties. Fifty-five S. Saintpaul isolates from German turkeys and turkey-derived food products isolated from 2000 to 2007 were analyzed by using antimicrobial agent, organic solvent, and disinfectant susceptibility tests, isoelectric focusing, detection of resistance determinants, plasmid profiling, pulsed-field gel electrophoresis (PFGE), and hybridization experiments. These isolates were compared to an outgroup consisting of 24 S. Saintpaul isolates obtained from humans and chickens in Germany and from poultry and poultry products (including turkeys) in Netherlands. A common core resistance pattern was detected for 27 German turkey and turkey product isolates. This pattern included resistance (full or intermediate) to ampicillin, amoxicillin-clavulanic acid, gentamicin, kanamycin, nalidixic acid, streptomycin, spectinomycin, and sulfamethoxazole and intermediate resistance or decreased susceptibility to ciprofloxacin (MIC, 2 or 1 μg/ml, respectively) and several third-generation cephalosporins (including ceftiofur and cefoxitin [MIC, 4 to 2 and 16 to 2 μg/ml, respectively]). These isolates had the same core resistance genotype, with bla TEM-1, aadB, aadA2, sul1, a Ser83→Glu83 mutation in the gyrA gene, and a chromosomal class 1 integron carrying the aadB-aadA2 gene cassette. Their XbaI, BlnI, and combined XbaI-BlnI PFGE patterns revealed levels of genetic similarity of 93, 75, and 90%, respectively. This study revealed that a multiresistant S. Saintpaul clonal line is widespread in turkeys and turkey products in Germany and was also detected among German human fecal and Dutch poultry isolates.

scholarly journals Use of Inverse PCR for Analysis of Class 1 Integrons Carrying an Unusual 3′ Conserved Segment Structure

2010 ◽  
Vol 55 (2) ◽  
pp. 943-945 ◽  
Author(s):  
Mei-Feng Lee ◽  
Chien-Fang Peng ◽  
Hui-Jine Hsu ◽  
Han-Siong Toh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Dna Sequencing ◽  
Salmonella Enterica ◽  
Southern Hybridization ◽  
Enterobacter Cloacae ◽  
Inverse Pcr ◽  
Chromosomal Dna ◽  
Pcr Assay ◽  
Class 1 Integrons ◽  
Class 1

ABSTRACTBy using inverse PCR and DNA sequencing, 13sul3-associated mutational integrons, 2 defective class 1 integrons, and 1qnrB2-associated complexsul1-type class 1 integrons were identified inSalmonella entericaserovar Choleraesuis,Pseudomonas aeruginosa, andEnterobacter cloacae, respectively. In addition, conjugation and Southern hybridization demonstrated that unusual class 1 integrons were located on plasmids or integrated into chromosomal DNA. Thus, an inverse PCR assay can be a valuable tool for the analysis of unusual structures of the 3′ conserved region of class 1 integrons.

Diversity of Gene Cassette Promoter Variants of Class 1 Integrons in Uropathogenic Escherichia coli

2013 ◽  
Vol 67 (5) ◽  
pp. 543-549 ◽  
Author(s):  
Quhao Wei ◽  
Xiaofei Jiang ◽  
Min Li ◽  
Gang Li ◽  
Qingfeng Hu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Cassette ◽  
Uropathogenic Escherichia Coli ◽  
Class 1 Integrons ◽  
Class 1

Multiple-Antibiotic Resistance in Salmonella enterica Serovars Isolated in Iran Harboring Class 1 Integrons

2015 ◽  
Vol 5 (2) ◽  
pp. 186-193 ◽  
Author(s):  
Bahareh Rajaei ◽  
Nahid Rad ◽  
Farzad Badmasti ◽  
Mohamad Razavi ◽  
Mohammad Aghasadeghi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Enterica ◽  
Multiple Antibiotic Resistance ◽  
Class 1 Integrons ◽  
Class 1

scholarly journals Highly Drug-Resistant Salmonella enterica Serovar Indiana Clinical Isolates Recovered from Broilers and Poultry Workers with Diarrhea in China

2016 ◽  
Vol 60 (3) ◽  
pp. 1943-1947 ◽  
Author(s):  
Jiansen Gong ◽  
Chengming Wang ◽  
Shourong Shi ◽  
Hongduo Bao ◽  
Chunhong Zhu ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Content Type ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Poultry Workers

Highly drug-resistantSalmonella entericaserovar Indiana became the most common serovar in broilers with diarrhea in China over the course of this study (15% in 2010 to 70% in 2014). While mostS. Indiana isolates (87%, 384/440) were resistant to 13 to 16 of the 16 antibiotics tested, 89% of non-S. Indiana isolates (528/595) were resistant to 0 to 6 antibiotics. Class 1 integrons and IncHI2-type plasmids were detected in allS. Indiana isolates, but only in 39% and 1% of non-S. Indiana isolates.

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