scholarly journals Emergence of Multidrug-Resistant Klebsiella pneumoniae Isolates Producing VIM-4 Metallo-β-Lactamase, CTX-M-15 Extended-Spectrum β-Lactamase, and CMY-4 AmpC β-Lactamase in a Tunisian University Hospital

2006 ◽  
Vol 50 (12) ◽  
pp. 4198-4201 ◽  
Author(s):  
Sonia Ktari ◽  
Guillaume Arlet ◽  
Basma Mnif ◽  
Valérie Gautier ◽  
Fouzia Mahjoubi ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Multidrug Resistant ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
University Hospital ◽  
Class 1 Integron ◽  
Pulsed Field ◽  
Extended Spectrum ◽  
Class 1

ABSTRACT Klebsiella pneumoniae clinical isolates resistant to carbapenems were recovered from 11 patients in the hospital of Sfax, Tunisia. The isolates were closely related as shown by pulsed-field gel electrophoresis, and they produced VIM-4 metallo-enzyme, CTX-M-15 extended-spectrum β-lactamase, and CMY-4 AmpC enzyme. The bla VIM-4 gene is part of a class 1 integron.

scholarly journals Clinical Isolates of Enterobacteriaceae Producing Extended-Spectrum β-Lactamases: Prevalence of CTX-M-3 at a Hospital in China

2003 ◽  
Vol 47 (2) ◽  
pp. 790-793 ◽  
Author(s):  
Hui Wang ◽  
Swathi Kelkar ◽  
Weiyuan Wu ◽  
Minjun Chen ◽  
John P. Quinn
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Citrobacter Freundii ◽  
Pulsed Field ◽  
Extended Spectrum

ABSTRACT The prevalence of extended-spectrum β-lactamase-producing strains was demonstrated in 5 of 44 (11.4%) Escherichia coli, 17 of 43 (39.5%) Klebsiella pneumoniae, 3 of 50 (6.0%) Enterobacter cloacae, and 2 of 25 (8.0%) Citrobacter freundii strains at a teaching hospital in China. Nineteen of these 27 strains expressed CTX-M-3 β-lactamase (pI 8.6). A subset of the clinical isolates expressing the CTX-M-3 enzyme, tested by pulsed-field gel electrophoresis, revealed multiple clones. Five isolates expressed a novel enzyme, SHV-43 (pI 8.0), which had two substitutions (Leu113Phe and Thr149Ser) compared with SHV-1.

scholarly journals Characterization of multidrug-resistant and extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains from Malaysian hospitals

2009 ◽  
Vol 58 (11) ◽  
pp. 1463-1469 ◽  
Author(s):  
King Ting Lim ◽  
Chew Chieng Yeo ◽  
Rohani Md Yasin ◽  
Ganeswrie Balan ◽  
Kwai Lin Thong
Keyword(s):  
Klebsiella Pneumoniae ◽  
Dna Fingerprinting ◽  
Antibiotic Resistance Gene ◽  
Multidrug Resistant ◽  
Pcr Detection ◽  
Management Problem ◽  
Class 1 Integron ◽  
Extended Spectrum ◽  
Class 1 ◽  
Encoding Genes

The emergence of multidrug-resistant (MDR) and extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae poses a serious antibiotic management problem as resistance genes are easily transferred from one organism to another. Fifty-one strains of K. pneumoniae isolated from sporadic cases in various hospitals throughout Malaysia were analysed by antimicrobial susceptibility testing, PCR detection of ESBL-encoding genes and DNA fingerprinting. Although 27 of the 51 K. pneumoniae strains were MDR (i.e. resistant to three or more classes of antibiotics), the majority of the strains (98 %) were sensitive to imipenem. PCR detection using ESBL gene-specific primers showed that 46 of the K. pneumoniae strains harboured bla SHV, 19 harboured bla CTX-M, 5 harboured bla OXA-1 and 4 harboured bla TEM-1. Class 1 integron-encoded intI1 integrase was detected in 21 of the 51 K. pneumoniae strains and amplification of the integron 5′CS region showed the presence of several known antibiotic resistance gene cassettes of various sizes. Results of conjugation and transformation experiments indicated that some of the ESBL-encoding genes (i.e. bla SHV, bla CTX-M and bla TEM-1) were transmissible and were likely plasmid-encoded. DNA fingerprinting using PFGE and PCR-based methods indicated that the 51 K. pneumoniae strains were genetically diverse and heterogeneous.

scholarly journals Detection of the novel IMP-38 among carbapenemase-producing Enterobacteriaceae in a university hospital, China

2014 ◽  
Vol 8 (08) ◽  
pp. 1044-1048 ◽  
Author(s):  
Zijuan Jian ◽  
Yanming Li ◽  
Wenen Liu ◽  
Hongling Li ◽  
Yunli Zhang ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Molecular Epidemiology ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
University Hospital ◽  
The Novel ◽  
Pulsed Field ◽  
Carbapenem Resistant ◽  
Neonatal Ward ◽  
Carbapenem Resistant Enterobacteriaceae

Introduction: This study set out to investigate the molecular epidemiology of carbapenemase-producing Enterobacteriaceae isolates collected from Xiang Ya Hospital, Hunan, China. Methodology: The carbapenemase genes from Enterobacteriaceae isolates were determined by PCR and sequencing. Relatedness of Klebsiella pneumoniae isolates was evaluated by pulsed-field gel electrophoresis. Results: Twenty-four out of 738 non-repetitive Enterobacteriaceae isolates harbored carbapenemase genes including IMP-38, a novel IMP-type metallo-enzyme. Nine IMP-38-producing isolates were shown to originate from the same clone and caused a small outbreak in the neonatal ward. Conclusions: IMP-38, a novel IMP-type metallo-enzyme, was one of the predominant types of carbapenemase in the clinical carbapenem-resistant Enterobacteriaceae isolates in our hospital.

scholarly journals First Report ofblaIMP-8in Raoultella planticola

2013 ◽  
Vol 58 (1) ◽  
pp. 593-595 ◽  
Author(s):  
Sung-Pin Tseng ◽  
Jann-Tay Wang ◽  
Chih-Yuan Liang ◽  
Pei-Shan Lee ◽  
Yee-Chun Chen ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Protein Loss ◽  
Class 1 Integron ◽  
Raoultella Planticola ◽  
Content Type ◽  
First Report ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTwo carbapenem-resistantRaoultella planticolaclinical isolates were isolated from patients with pneumonia and Port-A catheter-related bacteremia, respectively, in Taiwan. These isolates remained susceptible to fluoroquinolone, aminoglycoside, and colistin. Though the two isolates had the same antibiogram, plasmidic carbapenemaseblaIMP-8, class 1 integron cassette (dfrA12-orfF-aadA2), andqnrB2, they had different pulsed-field gel electrophoresis patterns, plasmid sizes, and outer membrane protein loss profiles. To our knowledge, this is the first report ofblaIMP-8found inR. planticola. Interestingly,blaIMP-8is the most common carbapenemase found inKlebsiella pneumoniaein Taiwan. In the literature, carbapenemase genes inR. planticolain each country were also found in carbapenem-resistantEnterobacteriaceaein the same country.

scholarly journals Antimicrobial Susceptibility and Characterization of Salmonella Isolates from Processed Bison Carcasses

2006 ◽  
Vol 72 (4) ◽  
pp. 3046-3049 ◽  
Author(s):  
Qiongzhen Li ◽  
Jerod A. Skyberg ◽  
Mohamed K. Fakhr ◽  
Julie S. Sherwood ◽  
Lisa K. Nolan ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Virulence Genes ◽  
Pulsed Field Gel Electrophoresis ◽  
Cross Contamination ◽  
Class 1 Integron ◽  
Pulsed Field ◽  
Class 1

ABSTRACT Seventeen Salmonella enterica serovar Hadar isolates recovered from bison were found to possess a range of virulence genes and resistance to tetracycline, gentamicin, sulfamethoxazole, and streptomycin simultaneously. A 1-kb class 1 integron containing the aadA1 gene was identified in all isolates. Pulsed-field gel electrophoresis found that all isolates were closely related, indicating the possibility of cross-contamination during processing.

Sign in / Sign up

Export Citation Format

Share Document