Method for Screening Compounds That Influence Virulence Gene Expression in Staphylococcus aureus
2009 ◽
Vol 54
(1)
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pp. 509-512
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Keyword(s):
ABSTRACT We present a simple assay to examine effects of compounds on virulence gene expression in the human pathogen Staphylococcus aureus. The assay employs transcriptional reporter strains carrying lacZ fused to central virulence genes. Compounds affecting virulence gene expression and activity of the agr locus are scored based on color change in the presence of a chromogenic β-galactosidase substrate. The assay can be used to screen for novel antivirulence compounds from many different sources, such as fungi, as demonstrated here.
2009 ◽
Vol 76
(1)
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pp. 303-309
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Keyword(s):
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2006 ◽
Vol 50
(4)
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pp. 1183-1194
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Keyword(s):
2019 ◽
Vol 1862
(7)
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pp. 734-741
2001 ◽
Vol 183
(12)
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pp. 3704-3711
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