scholarly journals Dissemination of a Carbapenem-Resistant Acinetobacter baumannii Strain Belonging to International Clone II/Sequence Type 2 and Harboring a Novel AbaR4-Like Resistance Island in Latvia

2012 ◽  
Vol 57 (2) ◽  
pp. 1069-1072 ◽  
Author(s):  
M. Saule ◽  
O. Samuelsen ◽  
U. Dumpis ◽  
A. Sundsfjord ◽  
A. Karlsone ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Sequence Type ◽  
Carbapenem Resistant ◽  
Resistance Island

scholarly journals First Description of Two Sequence Type 2 Acinetobacter baumannii Isolates Carrying OXA-23 Carbapenemase in Pagellus acarne Fished from the Mediterranean Sea near Bejaia, Algeria

2016 ◽  
Vol 60 (4) ◽  
pp. 2513-2515 ◽  
Author(s):  
Soumia Brahmi ◽  
Abdelaziz Touati ◽  
Axelle Cadière ◽  
Nassima Djahmi ◽  
Alix Pantel ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Mediterranean Sea ◽  
Sequence Type ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Selective Agar ◽  
Agar Media ◽  
The Mediterranean ◽  
First Time

ABSTRACTTo determine the occurrence of carbapenem-resistantAcinetobacter baumanniiin fish fished from the Mediterranean Sea near the Bejaia coast (Algeria), we studied 300 gills and gut samples that had been randomly and prospectively collected during 1 year. After screening on selective agar media, using PCR arrays and whole-genome sequencing, we identified for the first time two OXA-23-producingA. baumanniistrains belonging to the widespread sequence type 2 (ST2)/international clone II and harboring aminoglycoside-modifying enzymes [aac(6′)-Ib andaac(3′)-I genes].

scholarly journals First Report of an OXA-23 Carbapenemase-Producing Acinetobacter baumannii Clinical Isolate Related to Tn2006in Spain

2012 ◽  
Vol 57 (1) ◽  
pp. 589-591 ◽  
Author(s):  
P. Espinal ◽  
M. D. Macià ◽  
I. Roca ◽  
E. Gato ◽  
E. Ruíz ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Acinetobacter Baumannii ◽  
Clinical Isolate ◽  
Sequence Type ◽  
Content Type ◽  
First Report ◽  
Carbapenem Resistant

ABSTRACTA carbapenem-resistantAcinetobacter baumanniiclinical isolate belonging to European clone II and sequence type 2 was recovered from a patient in the Son Espases hospital in Mallorca, Spain. Genetic analysis showed the presence of theblaOXA-23gene in association with the widely disseminated transposon Tn2006. This is the first reported identification ofA. baumanniicarryingblaOXA-23in Spain.

Extensively drug-resistant community-acquired Acinetobacter baumannii sequence type 2 in a dog with urinary tract infection in Thailand

2018 ◽  
Vol 13 ◽  
pp. 33-34 ◽  
Author(s):  
Pattrarat Chanchaithong ◽  
Nuvee Prapasarakul ◽  
Nicole Sirisopit Mehl ◽  
Nipattra Suanpairintr ◽  
Komkrich Teankum ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Acinetobacter Baumannii ◽  
Sequence Type ◽  
Drug Resistant ◽  
Extensively Drug Resistant

scholarly journals Molecular Epidemiology of Emerging bla OXA-23-Like - and bla OXA-24-Like -Carrying Acinetobacter baumannii in Taiwan

2018 ◽  
Vol 62 (3) ◽  
Author(s):  
Shu-Chen Kuo ◽  
Wei-Cheng Huang ◽  
Tzu-Wen Huang ◽  
Hui-Ying Wang ◽  
Jui-Fen Lai ◽  
...  
Keyword(s):  
Molecular Epidemiology ◽  
Acinetobacter Baumannii ◽  
Resistance Genes ◽  
Aminoglycoside Resistance ◽  
S1 Nuclease ◽  
Content Type ◽  
Class A ◽  
Carbapenem Resistant ◽  
Aminoglycoside Resistance Genes ◽  
Resistance Island

ABSTRACT The rate of recovery of carbapenem-resistant Acinetobacter baumannii (CRAB) isolates has increased significantly in recent decades in Taiwan. This study investigated the molecular epidemiology of CRAB with a focus on the mechanisms of resistance and spread in isolates with bla OXA-23-like or bla OXA-24-like . All 555 CRAB isolates in our multicenter collection, which were recovered from 2002 to 2010, were tested for the presence of class A, B, and D carbapenemase genes. All isolates with bla OXA-23-like or bla OXA-24-like were subjected to pulsed-field gel electrophoresis, and 82 isolates (60 isolates with bla OXA-23-like and 22 isolates with bla OXA-24-like ) were selected for multilocus sequence typing to determine the sequence type (ST) and clonal group (CG) and for detection of additional β-lactamase and aminoglycoside resistance genes. The flanking regions of carbapenem and aminoglycoside resistance genes were identified by PCR mapping and sequencing. The localization of bla OXA was determined by S1 nuclease and I-CeuI assays. The numbers of CRAB isolates carrying bla OXA-23-like or bla OXA-24-like , especially those carrying bla OXA-23-like , increased significantly from 2008 onward. The bla OXA-23-like gene was carried by antibiotic resistance genomic island 1 (AbGRI1)-type structures located on plasmids and/or the chromosome in isolates of different STs (CG92 and novel CG786), whereas bla OXA-24-like was carried on plasmids in CRAB isolates of limited STs (CG92). No class A or B carbapenemase genes were identified. Multiple aminoglycoside resistance genes coexisted in CRAB. Tn 6180 -borne armA was found in 74 (90.2%) CRAB isolates, and 58 (70.7%) isolates had Tn 6179 upstream, constituting AbGRI3. bla TEM was present in 38 (46.3%) of the CRAB isolates tested, with 35 (92.1%) isolates containing bla TEM in AbGRI2-type structures, and 61% of ampC genes had IS Aba1 upstream. We conclude that the dissemination and spread of a few dominant lineages of CRAB containing various resistance island structures occurred in Taiwan.

scholarly journals Molecular Epidemiology of Carbapenem-Resistant Acinetobacter baumannii Isolated from War-Injured Patients from the Eastern Ukraine

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 579
Author(s):  
Paul G. Higgins ◽  
Ralf Matthias Hagen ◽  
Andreas Podbielski ◽  
Hagen Frickmann ◽  
Philipp Warnke
Keyword(s):  
Acinetobacter Baumannii ◽  
Core Genome ◽  
Acquired Resistance ◽  
Sequence Type ◽  
Carbapenem Resistant ◽  
Next Generation Sequencing Ngs ◽  
Injured Patients ◽  
Sequence Types ◽  
Generation Sequencing ◽  
Epidemiological Information

Recently, a total of 32 carbapenem- and fluoroquinolone-resistant Acinetobacter baumannii (Ab) isolates was isolated from war-injured patients who were treated at German Bundeswehr Hospitals, and preliminarily typed by “DiversiLab” repetitive elements sequence-based (rep-) PCR. Core genome-based sequence typing was also used to provide more detailed epidemiological information. From the clusters observed by rep-PCR, selected Ab strains were subjected to Next Generation Sequencing (NGS) in order to compare them with international outbreak-associated Ab strains and to identify MLST (multi-locus sequence type) lineages, as well as to identify known resistance genes. Accordingly, NGS indicated higher diversity than rep-PCR, but also confirmed likely transmission events. The identified acquired carbapenem-resistant genes comprised blaOXA-23, blaOXA-72 and blaGES-12, as well as various other intrinsic and acquired resistance-associated genetic elements. All isolates clustered with the previously identified international clonal lineages IC1, IC2, IC6 and IC7, with corresponding Pasteur sequence types ST1, ST2, ST78 and ST25, respectively. In conclusion, the assessment confirmed a broad spectrum of resistance-associated genes in Ab isolated from war-injured patients from the Eastern Ukraine, and provided the first insights into locally abundant clonal lineages.

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