scholarly journals Molecular Characterization of IMP-1-Producing Enterobacter cloacae Complex Isolates in Tokyo

2018 ◽  
Vol 62 (3) ◽  
Author(s):  
Kotaro Aoki ◽  
Sohei Harada ◽  
Koji Yahara ◽  
Yoshikazu Ishii ◽  
Daisuke Motooka ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Asia Pacific ◽  
Single Nucleotide ◽  
Content Type ◽  
Plasmid Analysis ◽  
Enterobacter Hormaechei ◽  
Clade 1 ◽  
Genetic Structures ◽  
Sequence Types

ABSTRACT Although KPC enzymes are most common among carbapenemases produced by Enterobacter cloacae complex globally, the epidemiology varies from one country to another. While previous studies have suggested that IMP enzymes are most common in Japan, detailed analysis has been scarce thus far. Here, we carried out a molecular epidemiological study and plasmid analysis of IMP-1-producing E. cloacae complex isolates collected from three hospitals in central Tokyo using whole-genome sequencing. Seventy-one isolates were classified into several sequence types (STs), and 49 isolates were identified as Enterobacter hormaechei ST78. Isolates of ST78 were divided into three clades by core-genome single nucleotide polymorphism (SNP)-based phylogenetic analysis. Whereas isolates of clade 3 were isolated from only one hospital, isolates of clade 1 and 2 were identified from multiple hospitals. Ten of 12 clade 1 isolates and 1 of 4 clade 2 isolates carried bla IMP-1 on IncHI2 plasmids, with high similarity of genetic structures. In addition, these plasmids shared backbone structures with IncHI2 plasmids carrying bla IMP reported from other countries of the Asia-Pacific region. All isolates of clade 3 except one carried bla IMP-1 in In1426 on IncW plasmids. An isolate of clade 3, which lacked IncW plasmids, carried bla IMP-1 in In1426 on an IncFIB plasmid. These observations suggest that IMP-producing E. cloacae complex isolates with a diversity of host genomic backgrounds have spread in central Tokyo, and they indicate the possible contribution of IncHI2 plasmids toward this phenomenon.

scholarly journals Characterization of Tn3000, a Transposon Responsible forblaNDM-1Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India

2015 ◽  
Vol 59 (12) ◽  
pp. 7387-7395 ◽  
Author(s):  
Juliana Coutinho Campos ◽  
Maria José Félix da Silva ◽  
Paulo Roberto Nascimento dos Santos ◽  
Elaine Menezes Barros ◽  
Mayne de Oliveira Pereira ◽  
...  
Keyword(s):  
Rectal Swab ◽  
Swab Sample ◽  
Illumina Platform ◽  
Content Type ◽  
History Of ◽  
Enterobacter Hormaechei ◽  
Travel Abroad ◽  
Genetic Structures ◽  
Different Parts

ABSTRACTInEnterobacteriaceae, theblaNDMgenes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producingEscherichia coliandEnterobacter hormaecheistrains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring theblaNDM-1gene, one from each strain, showed that they belonged to incompatibility groups IncFIIKand IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that theblaNDM-1gene may be transmitted by Tn3000in different parts of the world.

scholarly journals Characterization of Multiple NDM-1-Producing Enterobacteriaceae Isolates from the Same Patient

2015 ◽  
Vol 59 (6) ◽  
pp. 3648-3651 ◽  
Author(s):  
Nathalie Tijet ◽  
David Richardson ◽  
Gregory MacMullin ◽  
Samir N. Patel ◽  
Roberto G. Melano
Keyword(s):  
Escherichia Coli ◽  
Male Patient ◽  
Community Hospital ◽  
Enterobacter Cloacae ◽  
Blood Cultures ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Plasmid Analysis ◽  
Providencia Stuartii

ABSTRACTA male patient was admitted to a community hospital in Ontario, Canada, with an infected sacral ulcer after returning from India, where he was hospitalized. Carbapenem-resistantEscherichia coli(isolated from blood cultures),Enterobacter cloacae, andProvidencia stuartii(from urine samples), all positive forblaNDM-1, were recovered. Comparative NDM-1 plasmid analysis suggests both lateral plasmid transfer and independent acquisition of theblaNDM-1gene in these clinical isolates.

scholarly journals Clonal Dissemination of OXA-370-Producing Klebsiella pneumoniae in Rio de Janeiro, Brazil

2015 ◽  
Vol 59 (8) ◽  
pp. 4453-4456 ◽  
Author(s):  
Polyana Silva Pereira ◽  
Mirla Borghi ◽  
Carlos Felipe Machado de Araújo ◽  
Caio Augusto Martins Aires ◽  
Jane Cleide Ribeiro Oliveira ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Rio De Janeiro ◽  
Enterobacter Aerogenes ◽  
Enterobacter Cloacae ◽  
Polymyxin B ◽  
Rio Grande Do Sul ◽  
Content Type ◽  
Beta Lactamases ◽  
Plasmid Analysis ◽  
Enterobacter Hormaechei

ABSTRACTEnzymes of the OXA-48 family have become some of the most important beta-lactamases in the world. A new OXA-48 variant (OXA-370) was first described for anEnterobacter hormaecheistrain isolated in Rio Grande do Sul (southern region of Brazil) in 2013. Here we report detection of theblaOXA-370gene in 24 isolates belonging to threeEnterobacteriaceaespecies (22Klebsiella pneumoniaeisolates, 1Enterobacter cloacaeisolate, and 1Enterobacter aerogenesisolate) collected from five hospitals in Rio de Janeiro, Brazil, in 2013 and 2014. The isolates showed a multidrug resistance profile, and 12.5% were resistant to polymyxin B. BesidesblaOXA-370, no other carbapenemase genes were observed by PCR, whereasblaOXA-1was found in all isolates and 22 isolates (91.6%) possessedblaCTX-M-15. Molecular typing of theK. pneumoniaeisolates by pulsed-field gel electrophoresis (PFGE) showed the presence of two clonal groups, i.e., KpA (21 isolates) and KpB (1 isolate). KpA was characterized as sequence type 16 (ST16) and KpB as ST1041 by multilocus sequence typing (MLST). ST16 has been observed for KPC-producingK. pneumoniaein Rio de Janeiro. Plasmid analysis performed with six representative OXA-370-producing isolates showed plasmids harboring theblaOXA-370gene in all strains, ranging from 25 kb to 150 kb. This study suggests that there is an urgent need to investigate the presence of OXA-370 and dissemination of theK. pneumoniaeST16 clone carrying this gene in Brazil.

scholarly journals Prevalence and Molecular Characterization of mcr-1-Positive Salmonella Strains Recovered from Clinical Specimens in China

2017 ◽  
Vol 61 (5) ◽  
Author(s):  
Mingquan Cui ◽  
Jinfei Zhang ◽  
Zhen Gu ◽  
Ruichao Li ◽  
Edward Wai-chi Chan ◽  
...  
Keyword(s):  
Pulsed Field Gel Electrophoresis ◽  
Clinical Settings ◽  
Colistin Resistance ◽  
Content Type ◽  
Transmission Potential ◽  
Conjugative Plasmids ◽  
Plasmid Analysis ◽  
Antimicrobial Resistance Genes ◽  
Pfge Profiles

ABSTRACT The recently discovered colistin resistance element, mcr-1, adds to the list of antimicrobial resistance genes that rapidly erode the antimicrobial efficacy of not only the commonly used antibiotics but also the last-line agents of carbapenems and colistin. This study investigated the prevalence of the mobile colistin resistance determinant mcr-1 in Salmonella strains recovered from clinical settings in China and the transmission potential of mcr-1-bearing mobile elements harbored by such isolates. The mcr-1 gene was recoverable in 1.4% of clinical isolates tested, with the majority of them belonging to Salmonella enterica serotype Typhimurium. These isolates exhibited diverse pulsed-field gel electrophoresis (PFGE) profiles and high resistance to antibiotics other than colistin and particularly to cephalosporins. Plasmid analysis showed that mcr-1 was carried on a variety of plasmids with sizes ranging from ∼30 to ∼250 kb, among which there were conjugative plasmids of ∼30 kb, ∼60 kb, and ∼250 kb and nonconjugative plasmids of ∼140 kb, ∼180 kb, and ∼240 kb. Sequencing of representative mcr-1-carrying plasmids revealed that all conjugative plasmids belonged to the IncX4, IncI2, and IncHI2 types and were highly similar to the corresponding types of plasmids reported previously. Nonconjugative plasmids all belonged to the IncHI2 type, and the nontransferability of these plasmids was attributed to the loss of a region carrying partial or complete tra genes. Our data revealed that, similar to the situation in Escherichia coli, mcr-1 transmission in Salmonella was accelerated by various plasmids, suggesting that transmission of mcr-1-carrying plasmids between different species of Enterobacteriaceae may be a common event.

scholarly journals Characterization of the Enterobacter Phage vB_EclM_CIP9

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Klara Wang ◽  
Marielou G. Tamayo ◽  
Tiffany V. Penner ◽  
Bradley W. M. Cook ◽  
Deborah A. Court ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Opportunistic Pathogen ◽  
Multidrug Resistant ◽  
Open Reading Frames ◽  
Resistant Isolate ◽  
Content Type ◽  
Hospital Acquired Infections ◽  
Hospital Acquired ◽  
Reading Frames

Enterobacter cloacae is an opportunistic pathogen that causes hospital-acquired infections in immunocompromised patients. Here, we describe vB_EclM_CIP9, a novel Enterobacter phage that infects a multidrug-resistant isolate of E. cloacae. Phage vB_EclM_CIP9 is a myovirus that has a 174,924-bp genome, with 296 predicted open reading frames.

scholarly journals Detection and Characterization of VIM-31, a New Variant of VIM-2 with Tyr224His and His252Arg Mutations, in a Clinical Isolate of Enterobacter cloacae

2012 ◽  
Vol 56 (6) ◽  
pp. 3283-3287 ◽  
Author(s):  
Pierre Bogaerts ◽  
Carine Bebrone ◽  
Te-Din Huang ◽  
Warda Bouchahrouf ◽  
Yves DeGheldre ◽  
...  
Keyword(s):  
Catalytic Efficiency ◽  
Enterobacter Cloacae ◽  
Colonic Adenocarcinoma ◽  
Beta Lactam ◽  
Class 1 Integron ◽  
Content Type ◽  
New Variant ◽  
Class 1 ◽  
Blood Specimens

ABSTRACTWe report the first description of the metallo-β-lactamase VIM-31, a new variant of VIM-2 with Tyr224His and His252Arg mutations, inEnterobacter cloacae11236, which was isolated from blood specimens of a patient with colonic adenocarcinoma in Belgium.blaVIM-31was found on a class 1 integron located on a self-transferable but not typeable 42-kb plasmid. Compared to values published elsewhere for VIM-2, the purified VIM-31 enzyme showed weaker catalytic efficiency against all the tested beta-lactam agents (except for ertapenem), resulting from lowerkcat(except for ertapenem) and higherKmvalues for VIM-31.

scholarly journals Genetic and Biochemical Characterization of FRI-1, a Carbapenem-Hydrolyzing Class A β-Lactamase from Enterobacter cloacae

2015 ◽  
Vol 59 (12) ◽  
pp. 7420-7425 ◽  
Author(s):  
Laurent Dortet ◽  
Laurent Poirel ◽  
Samia Abbas ◽  
Saoussen Oueslati ◽  
Patrice Nordmann
Keyword(s):  
Biochemical Characterization ◽  
Enterobacter Cloacae ◽  
Conjugative Plasmid ◽  
Lower Sensitivity ◽  
Gene Encoding ◽  
Content Type ◽  
Class A ◽  
Encoding Gene ◽  
Lactamase Gene

ABSTRACTAnEnterobacter cloacaeisolate was recovered from a rectal swab from a patient hospitalized in France with previous travel to Switzerland. It was resistant to penicillins, narrow- and broad-spectrum cephalosporins, aztreonam, and carbapenems but remained susceptible to expanded-spectrum cephalosporins. Whereas PCR-based identification of the most common carbapenemase genes failed, the biochemical Carba NP test II identified an Ambler class A carbapenemase. Cloning experiments followed by sequencing identified a gene encoding a totally novel class A carbapenemase, FRI-1, sharing 51 to 55% amino acid sequence identity with the closest carbapenemase sequences. However, it shared conserved residues as a source of carbapenemase activity. Purified β-lactamase FRI-1 hydrolyzed penicillins, aztreonam, and carbapenems but spared expanded-spectrum cephalosporins. The 50% inhibitory concentrations (IC50s) of clavulanic acid and tazobactam were 10-fold higher than those found forKlebsiella pneumoniaecarbapenemase (KPC), IMI, and SME, leading to lower sensitivity of FRI-1 activity to β-lactamase inhibitors. TheblaFRI-1gene was located on a ca. 110-kb untypeable, transferable, and non-self-conjugative plasmid. A putative LysR family regulator-encoding gene at the 5′ end of the β-lactamase gene was identified, leading to inducible expression of theblaFRI-1gene.

scholarly journals Do Staphylococcus epidermidis Genetic Clusters Predict Isolation Sources?

2016 ◽  
Vol 54 (7) ◽  
pp. 1711-1719 ◽  
Author(s):  
Isaiah Tolo ◽  
Jonathan C. Thomas ◽  
Rebecca S. B. Fischer ◽  
Eric L. Brown ◽  
Barry M. Gray ◽  
...  
Keyword(s):  
Population Structure ◽  
Staphylococcus Epidermidis ◽  
Learning Algorithm ◽  
Mobile Element ◽  
Single Nucleotide ◽  
Content Type ◽  
Hospital Patients ◽  
Contaminant Sources ◽  
Genetic Clusters ◽  
Sequence Types

Staphylococcus epidermidisis a ubiquitous colonizer of human skin and a common cause of medical device-associated infections. The extent to which the population genetic structure ofS. epidermidisdistinguishes commensal from pathogenic isolates is unclear. Previously, Bayesian clustering of 437 multilocus sequence types (STs) in the international database revealed a population structure of six genetic clusters (GCs) that may reflect the species' ecology. Here, we first verified the presence of six GCs, including two (GC3 and GC5) with significant admixture, in an updated database of 578 STs. Next, a single nucleotide polymorphism (SNP) assay was developed that accurately assigned 545 (94%) of 578 STs to GCs. Finally, the hypothesis that GCs could distinguish isolation sources was tested by SNP typing and GC assignment of 154 isolates from hospital patients with bacteremia and those with blood culture contaminants and from nonhospital carriage. GC5 was isolated almost exclusively from hospital sources. GC1 and GC6 were isolated from all sources but were overrepresented in isolates from nonhospital and infection sources, respectively. GC2, GC3, and GC4 were relatively rare in this collection. No association was detected betweenfdh-positive isolates (GC2 and GC4) and nonhospital sources. Using a machine learning algorithm, GCs predicted hospital and nonhospital sources with 80% accuracy and predicted infection and contaminant sources with 45% accuracy, which was comparable to the results seen with a combination of five genetic markers (icaA, IS256,sesD[bhp],mecA, and arginine catabolic mobile element [ACME]). Thus, analysis of population structure with subgenomic data shows the distinction of hospital and nonhospital sources and the near-inseparability of sources within a hospital.

scholarly journals Genomic Study of blaIMI-Positive Enterobacter cloacae Complex in Singapore over a Five-Year Study Period

2020 ◽  
Vol 64 (8) ◽  
Author(s):  
Sophie Octavia ◽  
Tse Hsien Koh ◽  
Oon Tek Ng ◽  
Kalisvar Marimuthu ◽  
Indumathi Venkatachalam ◽  
...  
Keyword(s):  
Enterobacter Cloacae ◽  
Mobile Elements ◽  
Content Type ◽  
Genomic Study ◽  
Genomic Characterization ◽  
Enterobacter Cloacae Complex

ABSTRACT The blaIMI gene is rarely detected outside the Enterobacter genus. Genomic characterization of 87 blaIMI-positive Enterobacter cloacae complex members revealed that the largest phylogenomic clade was made up of E. cloacae subsp. cloacae (71.3%), followed by the newly described species E. bugandensis (13.8%), E. sichuanensis (10.3%), and E. roggenkampii (4.6%). IMI-1 was the predominant carbapenemase variant (86/87, 98.9%). All the blaIMI genes were associated with chromosomally integrated Xer-dependent integrative mobile elements (IMEXs), with two new variants detected.

scholarly journals Genomic Characterization of Enterobacter cloacae Isolates from China That Coproduce KPC-3 and NDM-1 Carbapenemases

2016 ◽  
Vol 60 (4) ◽  
pp. 2519-2523 ◽  
Author(s):  
Hong Du ◽  
Liang Chen ◽  
Kalyan D. Chavda ◽  
Ruchi Pandey ◽  
Haifang Zhang ◽  
...  
Keyword(s):  
Direct Repeat ◽  
Enterobacter Cloacae ◽  
Tertiary Hospital ◽  
Sequence Type ◽  
Class I ◽  
Content Type ◽  
Repeat Sequences ◽  
Genomic Characterization

ABSTRACTHere, we report twoEnterobacter cloacaesequence type 231 isolates coproducing KPC-3 and NDM-1 that have caused lethal infections in a tertiary hospital in China. TheblaNDM-1-harboring plasmids carry IncA/C2and IncR replicons, showing a mosaic plasmid structure, and theblaNDM-1is harbored on a novel class I integron-like element.blaKPC-3is located on a Tn3-ΔblaTEM-1-blaKPC-3-ΔTn1722element, flanked by two 9-bp direct-repeat sequences and harbored on an IncX6 plasmid.

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