Aspergillus fumigatus C-5 Sterol Desaturases Erg3A and Erg3B: Role in Sterol Biosynthesis and Antifungal Drug Susceptibility
2006 ◽
Vol 50
(2)
◽
pp. 453-460
◽
Keyword(s):
ABSTRACT Two erg3 genes encoding C-5 sterol desaturase enzymes (Erg3A and Erg3B) in Aspergillus fumigatus were characterized with respect to their nucleotide sequences and null mutant phenotypes. Targeted disruption of the erg3A and erg3B genes and a double gene knockout, erg3A − erg3B −, showed that they are not essential for A. fumigatus viability. Mutant phenotypes clearly showed that Erg3B is a C-5 sterol desaturase, but no apparent role for Erg3A in A. fumigatus ergosterol biosynthesis was found. Susceptibility to amphotericin B, itraconazole, fluconazole, voriconazole, and ketoconazole was not altered in isolates in which erg3A and erg3B were knocked out alone and in combination.
Keyword(s):
2019 ◽
Vol 20
(22)
◽
pp. 5628
◽
Keyword(s):
2005 ◽
Vol 49
(6)
◽
pp. 2536-2538
◽
Keyword(s):
2021 ◽
Vol 105
(3)
◽
pp. 1253-1268
2011 ◽
Vol 55
(6)
◽
pp. 2606-2611
◽
2015 ◽
Vol 21
(5)
◽
pp. 385-391
◽
2010 ◽
Vol 39
(2)
◽
pp. 182-187
◽