scholarly journals Species-Specific Cell Wall Binding Affinity of the S-Layer Proteins of Mosquitocidal Bacterium Bacillus sphaericus C3-41

2009 ◽  
Vol 75 (12) ◽  
pp. 3891-3895 ◽  
Author(s):  
Jia Li ◽  
Xiaomin Hu ◽  
Jianpin Yan ◽  
Zhiming Yuan
Keyword(s):  
Amino Acids ◽  
Cell Wall ◽  
Bacterial Cell ◽  
Cell Walls ◽  
Specific Binding ◽  
Distinct Type ◽  
Bacillus Sphaericus ◽  
Specific Cell ◽  
Bacterial Cell Walls ◽  
Species Specific

ABSTRACT The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring the S-layer subunits to the rigid cell wall layer via a distinct type of secondary cell wall polymer and that a motif of the recombinant SLH polypeptide comprising amino acids 152 to 210 (rSLH152-210) was essential for the stable binding of the S-layer with the bacterial cell walls. The quantitative assays revealed that the KD (equilibrium dissociation constant) values of rSLH152-210 and rSLH31-210 with purified cell wall sacculi were 1.11 × 10−6 M and 1.40 × 10−6 M, respectively. The qualitative assays demonstrated that the SLH domain of strain C3-41 could bind only to the cell walls or the cells treated with 5 M guanidinium hydrochloride of both toxic and nontoxic B. sphaericus strains but not to those from other bacteria, indicating the species-specific binding of the SLH polypeptide of B. sphaericus with bacterial cell walls.

The outer layer of the cell envelope of Halobacterium halobium

1969 ◽  
Vol 47 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Carolyn L. Marshall ◽  
A. J. Wicken ◽  
A. D. Brown
Keyword(s):  
Cell Wall ◽  
Chemical Analysis ◽  
Outer Layer ◽  
Bacterial Cell ◽  
Cell Walls ◽  
Cell Envelope ◽  
Halobacterium Halobium ◽  
A Cell ◽  
Bacterial Cell Walls

The outer layer of the cell envelope of Halobacterium halobium was isolated after suspending the envelope in either 1 M NaCl or 0.02 M MgCl2. Chemical analysis of the isolated, solubilized outer layer showed it to consist of protein or glycoprotein with about 3% RNA. No free or bound lipid was detected. No cytochromes were present in the outer layer. Components commonly associated with bacterial cell walls were absent.Chemical composition together with the marked instability of the outer layer in a slight ion deficit are not consistent with a function of this layer as a "cell wall" of the organism.

A SEROLOGICAL STUDY OF CELL WALLS OF CERTAIN LACTIC ACID BACTERIA

1962 ◽  
Vol 8 (5) ◽  
pp. 629-637
Author(s):  
K. L. Chung ◽  
Roma Z. Hawirko
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Intact Cell ◽  
Synthetic Medium ◽  
Specific Cell ◽  
Intact Cells ◽  
Common Antigens ◽  
Species Specific ◽  
Relationship Of ◽  
The Relationship

From three species of Lactobacillus and three species of Streptococcus, cultured in a synthetic medium, cell walls were isolated following sonic disintegration and purified by washing. Sera against each species were prepared by injecting three rabbits with cell walls, and three with intact cells. Reciprocal agglutination tests were carried out with unabsorbed and absorbed antisera. More kinds of antibodies were detected with cell-wall antisera than with intact-cell antisera. Many species in the two genera shared common antigens. S. faecalis was the exception. Certain antigens believed to be complex haptens in nature reacted with heterologous antisera. Haemagglutination of tanned erythrocytes sensitized with a particulate cell-wall suspension showed fewer cross reactions than agglutination of intact-cell suspensions.The evidence presented shows the possibility of using antisera against species-specific cell-wall antigens for the identification of these species. The relationship of these species is discussed.

scholarly journals Sham, Sam, And Bacterial Communication

1997 ◽  
Vol 5 (8) ◽  
pp. 8-9
Author(s):  
Lee van Hook
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Cell Walls ◽  
Piezoelectric Crystal ◽  
Acoustic Microscope ◽  
Bacterial Communication ◽  
Bacterial Cell Walls ◽  
Scanning Acoustic Microscope

A scanning acoustic microscope (SAM) - a nanomicrophone using a piezoelectric crystal, may be used to examine bacterial colonies, not just materials specimens to detect phonons and listen to propagating microfractures.Since bacterial cell walls are rigid structures, they undergo mechanical distortions when channels open and dose. This causes them to squeak and pop, each channel having its own sound. Channels and receptor molecules are all of different sizes and shapes, and therefore deform the cell wall in unique ways. This means that each channel makes a unique (if faint) sound when it passes a molecule through itself and this activity can be delected, Transport rates of uptake and excretion for the various compounds car then be calculated trom the intensity of the sounds.

scholarly journals Full color palette of fluorescentd-amino acids for in situ labeling of bacterial cell walls

2017 ◽  
Vol 8 (9) ◽  
pp. 6313-6321 ◽  
Author(s):  
Yen-Pang Hsu ◽  
Jonathan Rittichier ◽  
Erkin Kuru ◽  
Jacob Yablonowski ◽  
Erick Pasciak ◽  
...  
Keyword(s):  
Amino Acids ◽  
Bacterial Cell ◽  
Cell Walls ◽  
Bacterial Species ◽  
Color Palette ◽  
Full Color ◽  
Bacterial Cell Walls

Fluorescentd-amino acids (FDAAs) enable efficientin situlabeling of peptidoglycan in diverse bacterial species.

scholarly journals Bacterial Cell Wall-Induced Arthritis: Chemical Composition and Tissue Distribution of Four Lactobacillus Strains

2000 ◽  
Vol 68 (6) ◽  
pp. 3535-3540 ◽  
Author(s):  
Egle Šimelyte ◽  
Marja Rimpiläinen ◽  
Leena Lehtonen ◽  
Xiang Zhang ◽  
Paavo Toivanen
Keyword(s):  
Cell Wall ◽  
Bacterial Cell ◽  
Cell Walls ◽  
Lactobacillus Casei ◽  
Chemical Structure ◽  
Chronic Arthritis ◽  
Bacterial Cell Wall ◽  
Peptidoglycan Structure ◽  
Bacterial Cell Walls ◽  
Spleen And Lymph Nodes

ABSTRACT To study what determines the arthritogenicity of bacterial cell walls, cell wall-induced arthritis in the rat was applied, using four strains of Lactobacillus. Three of the strains used proved to induce chronic arthritis in the rat; all were Lactobacillus casei. The cell wall of Lactobacillus fermentum did not induce chronic arthritis. All arthritogenic bacterial cell walls had the same peptidoglycan structure, whereas that of L. fermentum was different. Likewise, all arthritogenic cell walls were resistant to lysozyme degradation, whereas the L. fermentum cell wall was lysozyme sensitive. Muramic acid was observed in the liver, spleen, and lymph nodes in considerably larger amounts after injection of an arthritogenicL. casei cell wall than following injection of a nonarthritogenic L. fermentum cell wall. The L. casei cell wall also persisted in the tissues longer than theL. fermentum cell wall. The present results, taken together with those published previously, underline the possibility that the chemical structure of peptidoglycan is important in determining the arthritogenicity of the bacterial cell wall.

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