scholarly journals Rapid Method for Isolation of Desiccation-Tolerant Strains and Xeroprotectants

2010 ◽  
Vol 76 (15) ◽  
pp. 5254-5262 ◽  
Author(s):  
J. J. Narv�ez-Reinaldo ◽  
I. Barba ◽  
J. Gonz�lez-L�pez ◽  
A. Tunnacliffe ◽  
M. Manzanera
Keyword(s):  
Compatible Solutes ◽  
Soil Samples ◽  
Environmental Implications ◽  
Air Drying ◽  
New Approach ◽  
Long Term Stability ◽  
Therapeutic Value ◽  
Natural Isolates ◽  
Incubation Method

ABSTRACT A novel biotechnological process has been developed for the isolation of desiccation-tolerant microorganisms and their xeroprotectants, i.e., compatible solutes involved in long-term stability of biomolecules in the dry state. Following exposure of soil samples to chloroform, we isolated a collection of desiccation-tolerant microorganisms. This collection was screened for the production of xeroprotectants by a variation of the bacterial milking (osmotic downshock) procedure and by a novel air-drying/rehydration (“dry milking”) incubation method. The resultant solutes were shown to protect both proteins and living cells against desiccation damage, thereby validating them as xeroprotectants. Nuclear magnetic resonance (NMR) analytical studies were performed to identify the xeroprotectants; synthetic mixtures of these compounds were shown to perform similarly to natural isolates in drying experiments with proteins and cells. This new approach has biotechnological and environmental implications for the identification of new xeroprotectants of commercial and therapeutic value.

scholarly journals Influence of Silane Pretreatment and Warm Air-Drying on Long-Term Composite Adaptation to Lithium Disilicate Ceramic

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 86
Author(s):  
Pa Pa Kay Khine ◽  
Antonin Tichy ◽  
Ahmed Abdou ◽  
Keiichi Hosaka ◽  
Yasunori Sumi ◽  
...  
Keyword(s):  
Resin Composite ◽  
Lithium Disilicate ◽  
Drying Methods ◽  
Gap Formation ◽  
Universal Adhesive ◽  
Air Drying ◽  
Swept Source ◽  
Long Term Stability ◽  
Interfacial Gap

Background: Repair bonding to lithium disilicate ceramic (LDS) remains an issue. This study examined whether the adaptation of a resin composite to LDS can be improved by a silane pretreatment and warm air-drying. Methods: LDS blocks (IPS e.max CAD) with prefabricated tapered cavities were bonded using a silane-containing universal adhesive (Clearfil Universal Bond Quick ER; UBQ) or the bonding agent of a two-step self-etch adhesive (Clearfil SE Bond 2), with and without a silane pretreatment (Clearfil Ceramic Primer; CP). CP and the adhesives were air-dried with normal air (23 ± 1 °C) or warm air (60 ± 5 °C), light-cured, and the cavities were filled with a flowable composite. Interfacial gap formation was evaluated using swept-source optical coherence tomography immediately after filling, after 24 h, 5000 and 10,000 thermal cycles, and an additional 1 year of water storage. Results: Without the silane pretreatment, all specimens soon detached from the cavities. Warm air-drying significantly decreased gap formation compared to normal air-dried groups (p < 0.001) and improved long-term stability (p < 0.001). The lowest gap formation was observed with UBQ when the silane pretreatment was combined with warm air-drying. Conclusions: Composite adaptation to LDS was insufficient without silanization, but it was stable in the long term if the silane pretreatment and warm air-drying were combined.

scholarly journals An approach for long-term, multi-probe Neuropixels recordings in unrestrained rats

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Thomas Zhihao Luo ◽  
Adrian Gopnik Bondy ◽  
Diksha Gupta ◽  
Verity Alexander Elliott ◽  
Charles D Kopec ◽  
...  
Keyword(s):  
Cost Effective ◽  
New Approach ◽  
Neural Recordings ◽  
Chronic Recording ◽  
Long Term Stability ◽  
Multiple Cycles ◽  
Single Unit Recordings ◽  
Freely Moving ◽  
High Yields

The use of Neuropixels probes for chronic neural recordings is in its infancy and initial studies leave questions about long-term stability and probe reusability unaddressed. Here, we demonstrate a new approach for chronic Neuropixels recordings over a period of months in freely moving rats. Our approach allows multiple probes per rat and multiple cycles of probe reuse. We found that hundreds of units could be recorded for multiple months, but that yields depended systematically on anatomical position. Explanted probes displayed a small increase in noise compared to unimplanted probes, but this was insufficient to impair future single-unit recordings. We conclude that cost-effective, multi-region, and multi-probe Neuropixels recordings can be carried out with high yields over multiple months in rats or other similarly sized animals. Our methods and observations may facilitate the standardization of chronic recording from Neuropixels probes in freely moving animals.

Intramolecular force-compensated hydrogel-based sensors with reduced response times

2019 ◽  
Vol 86 (4) ◽  
pp. 227-236 ◽  
Author(s):  
Simon Binder ◽  
Gerald Gerlach
Keyword(s):  
Response Time ◽  
Response Times ◽  
Ion Concentration ◽  
New Approach ◽  
Long Term Stability ◽  
Continuous Sensor ◽  
Successful Reduction ◽  
Force Compensation ◽  
Hysteresis Behaviour

AbstractThe method of intramolecular force compensation forms a new approach for reducing the response time of hydrogel-based chemical sensors and improving their long-term stability. The principle is based on a single bisensitive hydrogel, that fulfills both the function of a sensor and an actuator. This paper presents the measuring principle, the required bisensitive hydrogel and a sensor setup. The results show the desired bisensitivity of the sensor to the temperature and ion concentration as well as the successful reduction of the response time by more than 50 % compared to the conventional deflection method. The continuous sensor operation with the application of varying measuring solvents indicates a negligibly low hysteresis behaviour.

One-Step Surgical Correction of Transverse and Anteroposterior Maxillary Deficiencies Using Wire Fixation. A New Approach and Long-Term Stability

2017 ◽  
Vol 75 (7) ◽  
pp. 1498-1513
Author(s):  
Max Domingues Pereira ◽  
Vivian Farfel ◽  
Gabriela Pereira Ribeiro Prado ◽  
Lydia Masako Ferreira
Keyword(s):  
Surgical Correction ◽  
New Approach ◽  
Term Stability ◽  
Long Term Stability ◽  
Wire Fixation ◽  
One Step

scholarly journals An approach for long-term, multi-probe Neuropixels recordings in unrestrained rats

2020 ◽  
Author(s):  
Thomas Zhihao Luo ◽  
Adrian G. Bondy ◽  
Diksha Gupta ◽  
Verity A. Elliott ◽  
Charles D. Kopec ◽  
...  
Keyword(s):  
Cost Effective ◽  
New Approach ◽  
Neural Recordings ◽  
Chronic Recording ◽  
Long Term Stability ◽  
Multiple Cycles ◽  
Single Unit Recordings ◽  
Freely Moving ◽  
High Yields

AbstractThe use of Neuropixels probes for chronic neural recordings is in its infancy and initial studies leave questions about long-term stability and probe reusability unaddressed. Here we demonstrate a new approach for chronic Neuropixels recordings over a period of months in freely moving rats. Our approach allows multiple probes per rat and multiple cycles of probe reuse. We found that hundreds of units could be recorded for multiple months, but that yields depended systematically on anatomical position. Explanted probes displayed a small increase in noise compared to unimplanted probes, but this was insufficient to impair future single-unit recordings. We conclude that cost-effective, multi-region, and multi-probe Neuropixels recordings can be carried out with high yields over multiple months in rats or other similarly sized animals. Our methods and observations may facilitate the standardization of chronic recording from Neuropixels probes in freely moving animals.

New Trim Configurations for Laser Trimmed Thick-Film Resistors – Experimental Verification

2005 ◽  
Vol 2 (1) ◽  
pp. 19-24 ◽  
Author(s):  
Slawomir Kaminski ◽  
Edward Mis ◽  
Maciej Szymendera ◽  
Andrzej Dziedzic
Keyword(s):  
Thick Film ◽  
Experimental Verification ◽  
Electrical Potential ◽  
Low Frequency ◽  
Frequency Noise ◽  
New Approach ◽  
Long Term Stability ◽  
Pathway Length ◽  
Contact Shape

Laser trimming is the most effective and popular trimming method of thick-film and LTCC resistors at present. It is also still a subject of continuing theoretical and experimental analysis and optimization. Very recently a new approach to this process was suggested. It consists in replacing two-contact bar resistors by three-contact distributed structures trimmed by narrow cuts just around additional contact of different shape [1,2]. This paper presents experimental verification of such an approach. The relative trim characteristic and sensitivity are analyzed as a function of additional contact shape and cut length. Next long-term stability, pulse durability and low frequency noise are compared for two- and three-contact resistors versus trim pathway length. These investigations are completed by simulation of electrical potential distribution in two- and three-contact resistors with various length of trimming kerf.

scholarly journals Air-drying pretreatment effect on soil enzymatic activity  

2017 ◽  
Vol 63 (No. 1) ◽  
pp. 29-33 ◽  
Author(s):  
Moreira Rodrigo Santos ◽  
Chiba Marcio Koiti ◽  
Nunes Stefany Batista ◽  
Maria Isabella Clerici de
Keyword(s):  
Acid Phosphatase ◽  
Soil Quality ◽  
Mineral Fertilizer ◽  
Soil Samples ◽  
Air Drying ◽  
Soil Enzymatic Activity ◽  
Term Experiment ◽  
Arylsulfatase Activity ◽  
Long Term Experiment

Air-drying of soil samples is a common practice for all-purpose soil testing. However, it may cause the cessation of microbial activity changing the biochemical attributes. For this reason, field-moist samples are commonly used in the assessment of the enzyme activity in soils. This practice may, therefore, discourage the use of enzymes in soil quality evaluations. This study evaluated the effects of air-drying on cellulase, arylsulfatase and acid phosphatase activities in soil; the hypothesis was that the activities of these enzymes determined in air-dried soil samples are similar to those obtained at field-moist samples. Soil samples were collected (0–10 cm) in a long-term experiment that received two rates (10 and 20 t/ha) of sewage sludge and mineral fertilizer and was cropped with maize. Collected soil samples were split into two groups. In the first one, the enzymes were determined at field-moist samples, while in the second one, the samples were air-dried before enzymatic analyses. Acid phosphatase was significantly affected by air-drying while the arylsulfatase activity hardly changed. The results showed that the enzymes determined in air-dried soil samples hold the capacity to identify different organic management and can, potentially, be used as soil quality indicators.  

scholarly journals Monitoring the State of Charge of the Positive Electrolyte in a Vanadium Redox-Flow Battery with a Novel Amperometric Sensor

Batteries ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 5 ◽  
Author(s):  
Isabelle Kroner ◽  
Maik Becker ◽  
Thomas Turek
Keyword(s):  
Gas Diffusion ◽  
The State ◽  
State Of Charge ◽  
Vanadium Redox Flow Battery ◽  
Rotating Disc ◽  
New Approach ◽  
Long Term Stability ◽  
The Individual ◽  
Vanadium Redox

Vanadium redox-flow batteries are a promising energy storage technology due to their safety, long-term stability, and independent adjustability of power and capacity. However, the vanadium crossover through the membrane causes a self-discharge, which results in a capacity shift towards one half cell. This leads to a gradual decrease in its efficiency over time. Capacity balancing methods for compensation of this effect require a reliable online state of charge (SoC) monitoring. Most common methods cannot provide exact values of the individual concentration of each species in both electrolytes. In particular, the state of the positive electrolyte cannot yet be precisely determined. In this work, an amperometric SoC monitoring is proposed as a new approach. First, the suitability of the principle is investigated with a rotating disc electrode (RDE). Then, a sensor based on a gas diffusion layer (GDL) is developed and tested in the positive electrolyte. The dependencies between oxidative current and V(IV)-concentration are examined as well as those between reduction current and V(V)-concentration. Using both relationships, a reliable measurement of all relevant concentrations is possible.

Sign in / Sign up

Export Citation Format

Share Document