Real-Time PCR Assay forClostridium perfringensin Broiler Chickens in a Challenge Model of Necrotic Enteritis
2010 ◽
Vol 77
(3)
◽
pp. 1135-1139
◽
Keyword(s):
ABSTRACTWe compared ilealClostridium perfringensquantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis indicated that the bacterial counts of these two methods were highly correlated (R2= 0.845). We suggest that real-time PCR could be a replacement of the culture method for quantifyingC. perfringensin the intestinal tracts of broiler chickens.
2004 ◽
Vol 48
(2)
◽
pp. 556-560
◽
Keyword(s):
2006 ◽
Vol 69
(10)
◽
pp. 2504-2508
◽
Keyword(s):
2004 ◽
Vol 53
(7)
◽
pp. 617-622
◽
Keyword(s):
2014 ◽
Vol 80
(6)
◽
pp. 1941-1948
◽
Keyword(s):
2009 ◽
Vol 75
(22)
◽
pp. 6981-6985
◽
Keyword(s):