scholarly journals Deciphering the Diversities of Astroviruses and Noroviruses in Wastewater Treatment Plant Effluents by a High-Throughput Sequencing Method

2015 ◽  
Vol 81 (20) ◽  
pp. 7215-7222 ◽  
Author(s):  
B. Prevost ◽  
F. S. Lucas ◽  
K. Ambert-Balay ◽  
P. Pothier ◽  
L. Moulin ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Clinical Data ◽  
High Throughput ◽  
Human Population ◽  
High Throughput Sequencing ◽  
Treatment Plant ◽  
Enteric Viruses ◽  
Viral Diversity ◽  
Wwtp Effluent ◽  
Human Astroviruses

ABSTRACTAlthough clinical epidemiology lists human enteric viruses to be among the primary causes of acute gastroenteritis in the human population, their circulation in the environment remains poorly investigated. These viruses are excreted by the human population into sewers and may be released into rivers through the effluents of wastewater treatment plants (WWTPs). In order to evaluate the viral diversity and loads in WWTP effluents of the Paris, France, urban area, which includes about 9 million inhabitants (approximately 15% of the French population), the seasonal occurrence of astroviruses and noroviruses in 100 WWTP effluent samples was investigated over 1 year. The coupling of these measurements with a high-throughput sequencing approach allowed the specific estimation of the diversity of human astroviruses (human astrovirus genotype 1 [HAstV-1], HAstV-2, HAstV-5, and HAstV-6), 7 genotypes of noroviruses (NoVs) of genogroup I (NoV GI.1 to NoV GI.6 and NoV GI.8), and 16 genotypes of NoVs of genogroup II (NoV GII.1 to NoV GII.7, NoV GII.9, NoV GII.12 to NoV GII.17, NoV GII.20, and NoV GII.21) in effluent samples. Comparison of the viral diversity in WWTP effluents to the viral diversity found by analysis of clinical data obtained throughout France underlined the consistency between the identified genotypes. However, some genotypes were locally present in effluents and were not found in the analysis of the clinical data. These findings could highlight an underestimation of the diversity of enteric viruses circulating in the human population. Consequently, analysis of WWTP effluents could allow the exploration of viral diversity not only in environmental waters but also in a human population linked to a sewerage network in order to better comprehend viral epidemiology and to forecast seasonal outbreaks.

Unbiased analysis by high throughput sequencing of the viral diversity in fetal bovine serum and trypsin used in cell culture

Biologicals ◽  
2014 ◽  
Vol 42 (3) ◽  
pp. 145-152 ◽  
Author(s):  
Léa Gagnieur ◽  
Justine Cheval ◽  
Marlène Gratigny ◽  
Charles Hébert ◽  
Erika Muth ◽  
...  
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Bovine Serum ◽  
High Throughput Sequencing ◽  
Fetal Bovine Serum ◽  
Viral Diversity ◽  
Fetal Bovine

scholarly journals Membrane Bioreactor-Based Wastewater Treatment Plant in Saudi Arabia: Reduction of Viral Diversity, Load, and Infectious Capacity

Water ◽  
2017 ◽  
Vol 9 (7) ◽  
pp. 534 ◽  
Author(s):  
Muhammad Jumat ◽  
Nur Hasan ◽  
Poorani Subramanian ◽  
Colin Heberling ◽  
Rita Colwell ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Membrane Bioreactor ◽  
Treatment Plant ◽  
Viral Diversity

scholarly journals Advanced nitrogen removal from municipal wastewater treatment plant secondary effluent using a deep bed denitrification filter

2018 ◽  
Vol 77 (11) ◽  
pp. 2723-2732 ◽  
Author(s):  
Xiaowei Zheng ◽  
Shenyao Zhang ◽  
Jibiao Zhang ◽  
Deying Huang ◽  
Zheng Zheng
Keyword(s):  
Wastewater Treatment ◽  
Quartz Sand ◽  
High Throughput Sequencing ◽  
Municipal Wastewater ◽  
Nitrate Nitrogen ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Secondary Effluent ◽  
Municipal Wastewater Treatment ◽  
Microbial Distribution

Abstract With the improvement of wastewater discharge standards, wastewater treatment plants (WWTPs) are continually undergoing technological improvements to meet the evolving standards. In this study, a quartz sand deep bed denitrification filter (DBDF) was used to purify WWTP secondary effluent, utilizing high nitrate nitrogen concentrations and a low C/N ratio. Results show that more than 90% of nitrate nitrogen (NO3-N) and 75% of chemical oxygen demand (COD) could be removed by the 20th day of filtration. When the filter layer depth was set to 1,600 mm and the additional carbon source CH3OH was maintained at 30 mg L−1 COD (20 mg L−1 methanol), the total nitrogen (TN) and COD concentrations of DBDF effluent were stabilized below 5 and 30 mg L−1, respectively. Analysis of fluorescence revealed that DBDF had a stronger effect on the removal of dissolved organic matter (DOM), especially of aromatic protein-like substances. High throughput sequencing and qPCR results indicate a distinctly stratified microbial distribution for the main functional species in DBDF, with quartz sand providing a good environment for microbes. The phyla Proteobacteria, Bacteroidetes, and Chloroflexi were found to be the dominant species in DBDF.

scholarly journals Nitrifying and Denitrifying Microbial Communities in Centralized and Decentralized Biological Nitrogen Removing Wastewater Treatment Systems

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1688 ◽  
Author(s):  
Sara K. Wigginton ◽  
Elizabeth Q. Brannon ◽  
Patrick J. Kearns ◽  
Brittany V. Lancellotti ◽  
Alissa Cox ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Microbial Communities ◽  
High Throughput Sequencing ◽  
Treatment Plant ◽  
Onsite Wastewater Treatment Systems ◽  
N Removal ◽  
Composition And Structure ◽  
Management Approaches ◽  
Biological Nitrogen ◽  
Wastewater Treatment Systems

Biological nitrogen removal (BNR) in centralized and decentralized wastewater treatment systems is assumed to be driven by the same microbial processes and to have communities with a similar composition and structure. There is, however, little information to support these assumptions, which may impact the effectiveness of decentralized systems. We used high-throughput sequencing to compare the structure and composition of the nitrifying and denitrifying bacterial communities of nine onsite wastewater treatment systems (OWTS) and one wastewater treatment plant (WTP) by targeting the genes coding for ammonia monooxygenase (amoA) and nitrous oxide reductase (nosZ). The amoA diversity was similar between the WTP and OWTS, but nosZ diversity was generally higher for the WTP. Beta diversity analyses showed the WTP and OWTS promoted distinct amoA and nosZ communities, although there is a core group of N-transforming bacteria common across scales of BNR treatment. Our results suggest that advanced N-removal OWTS have microbial communities that are sufficiently distinct from those of WTP with BNR, which may warrant different management approaches.

scholarly journals Chemometric Assessment of Bulgarian Wastewater Treatment Plants’ Effluents

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4408
Author(s):  
Galina Yotova ◽  
Tony Venelinov ◽  
Stefan Tsakovski
Keyword(s):  
Water Quality ◽  
Wastewater Treatment ◽  
Surface Water ◽  
Suspended Solids ◽  
Treatment Plant ◽  
Surface Water Quality ◽  
Anthropogenic Sources ◽  
Multivariate Curve Resolution ◽  
Anthropogenic Activity ◽  
Wwtp Effluent

Surface water quality strongly depends on anthropogenic activity. Among the main anthropogenic sources of this activity are the wastewater treatment plant (WWTP) effluents. The discharged loads of nutrients and suspended solids could provoke serious problems for receiving water bodies and significantly alter the surface water quality. This study presents inventory analysis and chemometric assessment of WWTP effluents based on the mandatory monitoring data. The comparison between the Bulgarian WWTPs and previously reported data from other countries reveals that discharged loads from investigated WWTPs are lower. This is particularly valid for total suspended solids (TSS). The low TSS loads are the reason for the deviations of the typical calculated WWTP effluent ratios of Bulgarian WWTPs compared to the WWTPs worldwide. The performed multivariate analysis reveals the hidden factors that determine the content of WWTP effluents. The source apportioning based on multivariate curve resolution analysis provides detailed information for source contribution profiles of the investigated WWTP effluent loads and elucidate the difference between WWTPs included in this study.

Sign in / Sign up

Export Citation Format

Share Document