scholarly journals Moderately Thermophilic Magnetotactic Bacteria from Hot Springs in Nevada

2010 ◽  
Vol 76 (11) ◽  
pp. 3740-3743 ◽  
Author(s):  
Christopher T. Lef�vre ◽  
Fernanda Abreu ◽  
Marian L. Schmidt ◽  
Ulysses Lins ◽  
Richard B. Frankel ◽  
...  
Keyword(s):  
Hot Springs ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Magnetotactic Bacteria ◽  
Rrna Gene ◽  
C Cells ◽  
Gram Negative ◽  
Moderately Thermophilic ◽  
Rrna Gene Sequencing ◽  
A Chain

ABSTRACT Populations of a moderately thermophilic magnetotactic bacterium were discovered in Great Boiling Springs, Nevada, ranging from 32 to 63�C. Cells were small, Gram-negative, vibrioid to helicoid in morphology, and biomineralized a chain of bullet-shaped magnetite magnetosomes. Phylogenetically, based on 16S rRNA gene sequencing, the organism belongs to the phylum Nitrospirae.

scholarly journals Detection of Cardiobacterium valvarum in a patient with aortic valve infective endocarditis by broad-range PCR

2010 ◽  
Vol 59 (2) ◽  
pp. 231-234 ◽  
Author(s):  
Martina Vaněrková ◽  
Barbora Žaloudíková ◽  
Eva Němcová ◽  
Jana Juránková ◽  
Jiří Pol ◽  
...  
Keyword(s):  
Infective Endocarditis ◽  
Aortic Valve ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Negative Bacterium ◽  
Review Of The Literature ◽  
Gram Negative ◽  
Prosthetic Aortic Valve ◽  
Rrna Gene Sequencing

Cardiobacterium valvarum, a fastidious Gram-negative bacterium, was detected in the aortic valve of a previously healthy 63-year-old man by broad-range PCR and 16S rRNA gene sequencing. In contrast to the patients in five previously published cases, our patient had neither a congenital bicuspid nor a prosthetic aortic valve. Here, we present a case of C. valvarum native tricuspid aortic valve infective endocarditis and a review of the literature.

scholarly journals IN-VITRO ANTIMICROBIAL ACTIVITY OF BIOLOGICAL SYNTHESIZED SILVER NANOPARTICLES USING STENOTROPHOMONAS MALTOPHILIA STRAIN NS-24 FROM NON-RHIZOSPHERE SOIL

2020 ◽  
pp. 73-79
Author(s):  
SREENIVASA NAYAKA ◽  
BIDHAYAK CHAKRABORTY ◽  
DATTATREYA AIRODGI ◽  
MEGHASHYAMA P. BHAT ◽  
SHASHIRAJ K. NAGARAJA ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Stenotrophomonas Maltophilia ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Gram Negative ◽  
Uv Visible ◽  
Rrna Gene Sequencing ◽  
And Staphylococcus Aureus

Objective: The present goals of our study were biological synthesis, characterizations of silver nanoparticles, and evaluation of its antimicrobial activity against microbial pathogens like Escherichia coli, Enterococcus faecalis, Streptococcus pneumoniae and Staphylococcus aureus. Methods: The bacterial Strain NS-24 was isolated on nutrient agar medium and was selected for the synthesis of silver nanoparticles based on its gram-negative characteristics. The characterizations of silver nanoparticles were done by UV-Visible spectroscopy, Atomic Force Microscopy (AFM), High Resolution-Transmission Electron Microscopy (HR-TEM), Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDX), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). Later, the molecular characterization of the Strain NS-24 was done by DNA extraction and 16S rRNA gene sequencing. Results: The UV-visible spectrophotometric observation of the Strain NS-24 supernatant and AgNO3 solution showed maximum absorbance at 423 nm. The AFM data confirmed that the particles were polydispersed and spherical in shape. Additionally, the FTIR analysis revealed the IR spectral band patterning and TEM analyzes showed the size of biological AgNPs was in the range of 12.56 nm to 27.32 nm, with an average of 18.06 nm in size. Further, the 16S rRNA gene sequencing revealed the identity of Strain NS-24 as Stenotrophomonas maltophilia. The antimicrobial activity of AgNPs was studied on different gram-negative and gram-positive bacterial strains like Escherichia coli (MTCC 40), Enterococcus faecalis (MTCC 6845), Streptococcus pneumoniae (MTCC 8874) and Staphylococcus aureus (MTCC 2825), which showed good inhibition of their growth at varying concentrations of AgNPs against all the pathogens. Conclusion: Our findings showed that the synthesized AgNPs from the isolated bacterium was small in size and had profound antibacterial activity against pathogenic micro-organisms.

Geographical Distribution and Diversity of Moderately Thermophilic Members of the Thermoplasmatales

2007 ◽  
Vol 20-21 ◽  
pp. 405-408
Author(s):  
Rebecca Hawkes ◽  
Graham O'Hara ◽  
Peter D. Franzmann ◽  
Jason J. Plumb
Keyword(s):  
Phylogenetic Diversity ◽  
Sequence Similarity ◽  
Ferrous Sulphate ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Moderately Thermophilic ◽  
Thermophilic Species ◽  
Acidic Sites ◽  
Rrna Gene Sequencing

Oxidation of ferrous iron by moderately thermophilic species of the genus Ferroplasma is of considerable potential value in commercial bioleaching operations. A collection of strains was enriched and isolated from a number of natural or industrial acidic sites at 45 and 55°C. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that all strains were members of the Order Thermoplasmatales. Stains BH8 and BH12 showed 98% sequence similarity with Ferroplasma acidiphilum strain YT and DR1 respectively. Strains BH7 and BH10 were closely related to the moderately thermophilic species Ferroplasma cupricumulans. Strain BH9, a moderately thermophilic organotroph, was unrelated to previously described species and probably represents a novel genus. Strains BH8 and BH12 showed some unique physiological differences to the type species F. acidiphilum. Unlike F. acidiphilum, both strains were moderately thermophilic with a temperature range for strains BH8 and BH12 of 24 to 61°C and 27 to 49°C respectively. Strain BH12 grew organotrophically on Darland’s Medium containing glucose and yeast extract and chemomixotrophically on Darland’s Medium supplemented with ferrous sulphate. Moderately thermophilic species of Ferroplasma and Ferroplasma-like genera appear widely distributed geographically and possess considerable physiological and phylogenetic diversity that may benefit industrial bioleaching processes.

scholarly journals In silico analysis of 16S rRNA gene sequencing based methods for identification of medically important aerobic Gram-negative bacteria

2011 ◽  
Vol 60 (9) ◽  
pp. 1281-1286 ◽  
Author(s):  
Jade L. L. Teng ◽  
Ming-Yiu Yeung ◽  
Geoffrey Yue ◽  
Rex K. H. Au-Yeung ◽  
Eugene Y. H. Yeung ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequencing ◽  
In Silico Analysis ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Rrna Gene Sequencing ◽  
Silico Analysis

scholarly journals Hymenobacter xinjiangensis sp. nov., a radiation-resistant bacterium isolated from the desert of Xinjiang, China

2007 ◽  
Vol 57 (8) ◽  
pp. 1752-1756 ◽  
Author(s):  
Qiuju Zhang ◽  
Cong Liu ◽  
Yali Tang ◽  
Guoling Zhou ◽  
Ping Shen ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Optimum Growth ◽  
Gram Negative ◽  
Radiation Resistant ◽  
Rrna Gene Sequencing ◽  
Sequence Similarities

A Gram-negative, non-motile, rod-shaped and pink-pigmented bacterium, designated strain X2-1gT, was isolated from a mixture of sand samples collected from the desert of Xinjiang, China, after exposure of the sand to 8 kGy gamma radiation. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that this isolate represents a novel member of the genus Hymenobacter, with low sequence similarities (<97 %) to recognized Hymenobacter species. Biolog GN2 assays supported this conclusion. Optimum growth was observed at pH 7 and 28 °C. The strain contained MK-7 as the predominant menaquinone and the major fatty acids were iso-C15 : 0 (19.5 %), C16 : 1 ω7c/iso-C15 : 0 2-OH (20.2 %), C16 : 1 ω5c (10.6 %), C16 : 0 (6.2 %), anteiso-C17 : 1 B/iso-C17 : 1 I (8.5 %) and C18 : 0 (6.5 %). The DNA G+C content was 54 mol% (T m). On the basis of the polyphasic evidence presented, it is proposed that strain X2-1gT represents a novel species of the genus Hymenobacter, for which the name Hymenobacter xinjiangensis sp. nov. is proposed. The type strain is X2-1gT (=CCTCC AB 206080T =IAM 15452T).

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